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The map label for this gene is metG

Identifier: 119899492

GI number: 119899492

Start: 3525107

End: 3527278

Strand: Direct

Name: metG

Synonym: azo3203

Alternate gene names: 119899492

Gene position: 3525107-3527278 (Clockwise)

Preceding gene: 119899491

Following gene: 119899493

Centisome position: 80.55

GC content: 66.16

Gene sequence:

>2172_bases
ATGTCCCGCAAGATCCTCGTCACCAACGCCCTGCCCTACGCCAACGGCGACATCCACCTCGGCCACCTCGTCGGCTACAT
CCAGGGCGACATCTGGGTGCGATTCCAGCGCATGCGCGGCAACTCGGTGCACTACGTGTGCGCCGACGATACCCACGGCA
CGCCGATCATGCTGCGCGCGGAGAAGGAAGGCATCACCCCCGAACAGCTGATCGACCGGGTGCATGGCGAGCACCTGCGC
GACTTCACCGACTTCGGCGTCGGCTTCGATCACTACCACTCCACCCACAGCGCCGAAAACCGCGCTTACGCCGAAGAGAT
CTACGGCAAGCTGCTCGGTGCGGGCTTCATCGAAACGCGCTCGATCGAGCAGTTCTACGACCCGGTCAAGGAAATGTTCC
TGCCGGACCGCTTCATCAAGGGCGAATGCCCCAAGTGCGGGGCCGGCGACCAGTACGGCGACAACTGCGAGGTCTGTGGC
GCCGCCTACGCGCCCACCGAGCTGAAGAATCCCTACTCCGCGGTGTCCGGCGCAAAGCCAGTGCTGCGCACCAGTGAGCA
CTACTTCTTCCGCCTGTCCGACCCGCGCGCGGTCGAGTTCCTGCGCGACTGGACGCGCGGCACCAACGCCGGCGGCGAAC
GCCGCCTGCAGGCCGAAGCGGCCAACAAGATGAAGGAATGGCTCGGCGAGGCGGGCGAGAACAAGCTCTCGGACTGGGAC
ATCTCGCGCGACGCGCCCTACTTCGGCTTCGAGATTCCCGGCGCGCCCGGCAAGTATTTCTACGTCTGGCTGGACGCCCC
GATCGGCTACCTCGCCAGCTTCCGCCACTATGCCGAACGCCGCGGCGACATCGCCGTGGCGGACTACACCGACGCCGCGC
GCGCGGAAAGCCATGGCACCGAGCTGGTGCACTTCATCGGCAAGGACATCCTCTACTTCCACGCGCTGTTCTGGCCGGCG
ATGCTGAAATTCGCCGGCTACCGCACGCCCACCCAGCTCTGCGTGAACGGTTTCCTGACGGTGGACGGCGCCAAGATGTC
CAAGAGCCGCGGCACCTTCATCACCGCGCGCTCCTACATCGTGCAGGGCCTCAACCCGGAATGGCTGCGCTACTACTTCG
CCAGCAAGTCCAACGGCACCATGGAAGACGTCGATCTCAACCTCGACGACATGATCGCCAAGGTCAATTCCGACCTGGTC
GGCAAGTACGTGAACATCGCCAGCCGCTGCGCGGGCTTCATCGCCAAGCGCTTCGACGGCAAGCTCGGCGCGAGCGACCC
GAAGGCCACCGCCGACTTCGAATCCGCCTACGAATCCGCGCAGATCGCCCAGGCTTACGACGAACGCGACTACGGCCGCG
CGCTGCGCGAGATCATGCGGCTGGCCGACCTGGCCAACCAGTACGTCAACGACCACAAGCCGTGGGAACTCGCCAAGCAG
GACGGCCAGGACGCGCAGCTTCACGTGGTCTGCAGCACCGCGCTGACGCTGTTCCGCGACCTGACGCTGTACCTCAAGCC
GGTGCTGCCCGGGCTGGCCGCCAAGGTGGAAGCCTTTCTCGCGCTGCCGCCGCTGCAGTGGAACGACAGCTGGAAGCCGC
TGCCGGCCGGGCACGCGATCACCGCGTACCAGCACCTGATGACGCGCGTCGAACGCAAGCAGATCGACGCCCTGCTGGAA
GCCAACCGCGAATCCCTGGCACCGGCTGCGGCCACGGCGCCCGCGAAGGACGCCAAGCCGGCCAAGGAAGCGGGTTCGCA
GCAGCGCCACGCCGAAAAGCAGCAGCACGCGGCCGGCGTCAGCGAAACCGCTTCGCCGCACATCTCGATCGACGACTTCA
CCAAGGTCGATCTGCGCATCGCGAAGATCGTGGATGCGCAGCACGTCGAAGGCGCCGACAAACTCATCCGGCTGCAGCTC
GACATCGGTGAAACCGACGAAGCGGGCAATCCGAAGCCGCGCCAGGTCTTCGCCGGCATCAAGTCCGCCTACGACCCCGC
GACGCTGGTCGGCCGGCTGACGGTGATGGTGGCCAACCTCGCCCCGCGCAAGATGAAGTTCGGTATGAGCGAAGGCATGG
TGCTCGCGGCGTCCGATCCGGACGGCAAGACCGGCGGGCTCTACATCCTCGCGCCCGACGAGGGCGCGCAGGCGGGCATG
CGGGTCAAGTAA

Upstream 100 bases:

>100_bases
GCTGCGCGCGATCTGATCCATCACGGGAGGCGAGGATGGGCGCGGGAAGCATGGCGCGCTTTGTTAAACTCGCGGCTTTG
ATTCCAGCCCGCCTCCCGCC

Downstream 100 bases:

>100_bases
GCCGTCCGCCGTGCTTTAACCAAAGTTCTACGTGCCGCGGGTGGACAAAGTCCGCCCGCTGGCCGAAAAATAGCAGCACC
TTCCCACCCGTCCCCGCCAT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 723; Mature: 722

Protein sequence:

>723_residues
MSRKILVTNALPYANGDIHLGHLVGYIQGDIWVRFQRMRGNSVHYVCADDTHGTPIMLRAEKEGITPEQLIDRVHGEHLR
DFTDFGVGFDHYHSTHSAENRAYAEEIYGKLLGAGFIETRSIEQFYDPVKEMFLPDRFIKGECPKCGAGDQYGDNCEVCG
AAYAPTELKNPYSAVSGAKPVLRTSEHYFFRLSDPRAVEFLRDWTRGTNAGGERRLQAEAANKMKEWLGEAGENKLSDWD
ISRDAPYFGFEIPGAPGKYFYVWLDAPIGYLASFRHYAERRGDIAVADYTDAARAESHGTELVHFIGKDILYFHALFWPA
MLKFAGYRTPTQLCVNGFLTVDGAKMSKSRGTFITARSYIVQGLNPEWLRYYFASKSNGTMEDVDLNLDDMIAKVNSDLV
GKYVNIASRCAGFIAKRFDGKLGASDPKATADFESAYESAQIAQAYDERDYGRALREIMRLADLANQYVNDHKPWELAKQ
DGQDAQLHVVCSTALTLFRDLTLYLKPVLPGLAAKVEAFLALPPLQWNDSWKPLPAGHAITAYQHLMTRVERKQIDALLE
ANRESLAPAAATAPAKDAKPAKEAGSQQRHAEKQQHAAGVSETASPHISIDDFTKVDLRIAKIVDAQHVEGADKLIRLQL
DIGETDEAGNPKPRQVFAGIKSAYDPATLVGRLTVMVANLAPRKMKFGMSEGMVLAASDPDGKTGGLYILAPDEGAQAGM
RVK

Sequences:

>Translated_723_residues
MSRKILVTNALPYANGDIHLGHLVGYIQGDIWVRFQRMRGNSVHYVCADDTHGTPIMLRAEKEGITPEQLIDRVHGEHLR
DFTDFGVGFDHYHSTHSAENRAYAEEIYGKLLGAGFIETRSIEQFYDPVKEMFLPDRFIKGECPKCGAGDQYGDNCEVCG
AAYAPTELKNPYSAVSGAKPVLRTSEHYFFRLSDPRAVEFLRDWTRGTNAGGERRLQAEAANKMKEWLGEAGENKLSDWD
ISRDAPYFGFEIPGAPGKYFYVWLDAPIGYLASFRHYAERRGDIAVADYTDAARAESHGTELVHFIGKDILYFHALFWPA
MLKFAGYRTPTQLCVNGFLTVDGAKMSKSRGTFITARSYIVQGLNPEWLRYYFASKSNGTMEDVDLNLDDMIAKVNSDLV
GKYVNIASRCAGFIAKRFDGKLGASDPKATADFESAYESAQIAQAYDERDYGRALREIMRLADLANQYVNDHKPWELAKQ
DGQDAQLHVVCSTALTLFRDLTLYLKPVLPGLAAKVEAFLALPPLQWNDSWKPLPAGHAITAYQHLMTRVERKQIDALLE
ANRESLAPAAATAPAKDAKPAKEAGSQQRHAEKQQHAAGVSETASPHISIDDFTKVDLRIAKIVDAQHVEGADKLIRLQL
DIGETDEAGNPKPRQVFAGIKSAYDPATLVGRLTVMVANLAPRKMKFGMSEGMVLAASDPDGKTGGLYILAPDEGAQAGM
RVK
>Mature_722_residues
SRKILVTNALPYANGDIHLGHLVGYIQGDIWVRFQRMRGNSVHYVCADDTHGTPIMLRAEKEGITPEQLIDRVHGEHLRD
FTDFGVGFDHYHSTHSAENRAYAEEIYGKLLGAGFIETRSIEQFYDPVKEMFLPDRFIKGECPKCGAGDQYGDNCEVCGA
AYAPTELKNPYSAVSGAKPVLRTSEHYFFRLSDPRAVEFLRDWTRGTNAGGERRLQAEAANKMKEWLGEAGENKLSDWDI
SRDAPYFGFEIPGAPGKYFYVWLDAPIGYLASFRHYAERRGDIAVADYTDAARAESHGTELVHFIGKDILYFHALFWPAM
LKFAGYRTPTQLCVNGFLTVDGAKMSKSRGTFITARSYIVQGLNPEWLRYYFASKSNGTMEDVDLNLDDMIAKVNSDLVG
KYVNIASRCAGFIAKRFDGKLGASDPKATADFESAYESAQIAQAYDERDYGRALREIMRLADLANQYVNDHKPWELAKQD
GQDAQLHVVCSTALTLFRDLTLYLKPVLPGLAAKVEAFLALPPLQWNDSWKPLPAGHAITAYQHLMTRVERKQIDALLEA
NRESLAPAAATAPAKDAKPAKEAGSQQRHAEKQQHAAGVSETASPHISIDDFTKVDLRIAKIVDAQHVEGADKLIRLQLD
IGETDEAGNPKPRQVFAGIKSAYDPATLVGRLTVMVANLAPRKMKFGMSEGMVLAASDPDGKTGGLYILAPDEGAQAGMR
VK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=575, Percent_Identity=29.3913043478261, Blast_Score=241, Evalue=1e-63,
Organism=Homo sapiens, GI24308436, Length=544, Percent_Identity=28.125, Blast_Score=172, Evalue=1e-42,
Organism=Escherichia coli, GI1788432, Length=729, Percent_Identity=52.5377229080933, Blast_Score=753, Evalue=0.0,
Organism=Escherichia coli, GI1789455, Length=115, Percent_Identity=33.0434782608696, Blast_Score=63, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17540794, Length=670, Percent_Identity=27.910447761194, Blast_Score=248, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI212645096, Length=535, Percent_Identity=24.2990654205607, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71992781, Length=352, Percent_Identity=25.8522727272727, Blast_Score=102, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6321704, Length=566, Percent_Identity=28.2685512367491, Blast_Score=243, Evalue=9e-65,
Organism=Saccharomyces cerevisiae, GI6321610, Length=564, Percent_Identity=25.177304964539, Blast_Score=143, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24655311, Length=577, Percent_Identity=28.7694974003466, Blast_Score=228, Evalue=1e-59,
Organism=Drosophila melanogaster, GI21358483, Length=549, Percent_Identity=25.1366120218579, Blast_Score=126, Evalue=5e-29,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_AZOSB (A1KAG3)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_934705.1
- ProteinModelPortal:   A1KAG3
- STRING:   A1KAG3
- GeneID:   4607848
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo3203
- eggNOG:   COG0143
- HOGENOM:   HBG721667
- OMA:   CEAVPES
- PhylomeDB:   A1KAG3
- ProtClustDB:   PRK00133
- BioCyc:   ASP62928:AZO3203-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 80120; Mature: 79989

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 348-348

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKILVTNALPYANGDIHLGHLVGYIQGDIWVRFQRMRGNSVHYVCADDTHGTPIMLRA
CCCEEEEEECCCCCCCCEEHHHHHHHHCCCEEEEEEECCCCEEEEEECCCCCCCEEEEEE
EKEGITPEQLIDRVHGEHLRDFTDFGVGFDHYHSTHSAENRAYAEEIYGKLLGAGFIETR
CCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHH
SIEQFYDPVKEMFLPDRFIKGECPKCGAGDQYGDNCEVCGAAYAPTELKNPYSAVSGAKP
HHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHCCCCC
VLRTSEHYFFRLSDPRAVEFLRDWTRGTNAGGERRLQAEAANKMKEWLGEAGENKLSDWD
HHCCCCCEEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
ISRDAPYFGFEIPGAPGKYFYVWLDAPIGYLASFRHYAERRGDIAVADYTDAARAESHGT
CCCCCCCCCEECCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCH
ELVHFIGKDILYFHALFWPAMLKFAGYRTPTQLCVNGFLTVDGAKMSKSRGTFITARSYI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCEEEECCCCCCCCCCCEEEEHHHH
VQGLNPEWLRYYFASKSNGTMEDVDLNLDDMIAKVNSDLVGKYVNIASRCAGFIAKRFDG
HCCCCHHHHHHHHHCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KLGASDPKATADFESAYESAQIAQAYDERDYGRALREIMRLADLANQYVNDHKPWELAKQ
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHC
DGQDAQLHVVCSTALTLFRDLTLYLKPVLPGLAAKVEAFLALPPLQWNDSWKPLPAGHAI
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHH
TAYQHLMTRVERKQIDALLEANRESLAPAAATAPAKDAKPAKEAGSQQRHAEKQQHAAGV
HHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHCCC
SETASPHISIDDFTKVDLRIAKIVDAQHVEGADKLIRLQLDIGETDEAGNPKPRQVFAGI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHH
KSAYDPATLVGRLTVMVANLAPRKMKFGMSEGMVLAASDPDGKTGGLYILAPDEGAQAGM
HHCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEECCCCCCCCCEEEEECCCCCCCCC
RVK
CCC
>Mature Secondary Structure 
SRKILVTNALPYANGDIHLGHLVGYIQGDIWVRFQRMRGNSVHYVCADDTHGTPIMLRA
CCEEEEEECCCCCCCCEEHHHHHHHHCCCEEEEEEECCCCEEEEEECCCCCCCEEEEEE
EKEGITPEQLIDRVHGEHLRDFTDFGVGFDHYHSTHSAENRAYAEEIYGKLLGAGFIETR
CCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHH
SIEQFYDPVKEMFLPDRFIKGECPKCGAGDQYGDNCEVCGAAYAPTELKNPYSAVSGAKP
HHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHCCCCC
VLRTSEHYFFRLSDPRAVEFLRDWTRGTNAGGERRLQAEAANKMKEWLGEAGENKLSDWD
HHCCCCCEEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
ISRDAPYFGFEIPGAPGKYFYVWLDAPIGYLASFRHYAERRGDIAVADYTDAARAESHGT
CCCCCCCCCEECCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCH
ELVHFIGKDILYFHALFWPAMLKFAGYRTPTQLCVNGFLTVDGAKMSKSRGTFITARSYI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCEEEECCCCCCCCCCCEEEEHHHH
VQGLNPEWLRYYFASKSNGTMEDVDLNLDDMIAKVNSDLVGKYVNIASRCAGFIAKRFDG
HCCCCHHHHHHHHHCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KLGASDPKATADFESAYESAQIAQAYDERDYGRALREIMRLADLANQYVNDHKPWELAKQ
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHC
DGQDAQLHVVCSTALTLFRDLTLYLKPVLPGLAAKVEAFLALPPLQWNDSWKPLPAGHAI
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHH
TAYQHLMTRVERKQIDALLEANRESLAPAAATAPAKDAKPAKEAGSQQRHAEKQQHAAGV
HHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHCCC
SETASPHISIDDFTKVDLRIAKIVDAQHVEGADKLIRLQLDIGETDEAGNPKPRQVFAGI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHH
KSAYDPATLVGRLTVMVANLAPRKMKFGMSEGMVLAASDPDGKTGGLYILAPDEGAQAGM
HHCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEECCCCCCCCCEEEEECCCCCCCCC
RVK
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA