Click here to switch to the map view.

The map label for this gene is wcaJ [H]

Identifier: 119899483

GI number: 119899483

Start: 3509439

End: 3510821

Strand: Reverse

Name: wcaJ [H]

Synonym: azo3193

Alternate gene names: 119899483

Gene position: 3510821-3509439 (Counterclockwise)

Preceding gene: 119899485

Following gene: 119899482

Centisome position: 80.23

GC content: 60.95

Gene sequence:

>1383_bases
ATGTTGAAGGTCTTCAGCCATTACCTGCCGTCCCATACGGTACAGCAGATTCTGTTCGACGCGCTGCTCTTGTTCGTGGC
TGTGCTGTTGGCCGTTTTCATGCAGTTGCAGACCGAGATTATTGAATGGCGGGTGCTGGTTCCATCCGCGATGTTCTTTG
CTCTGTCCATGGTAGTGCTCAACACCGCGATGGGGCTTTATCGCTCCGCGGTGCGCGAGTCGCAGCGGGGCGCTGTGGCC
CGAGTCGTGCTCTCGGTCGTTGTCAGTGTCCCGGTGGCCTACGGGGTGTTCAGCGTGCTGCCTTGGGCCGAGCCGTCAAA
TGTTTCGGTGCAGATATCCGTCCTGGTTTTGCTCGGGTTCGTCTTGGTGATGCGTGGGCTGGTTAACCGCAGGCAAGCGT
CGGCGCTGTTTGCGCCCCGTGTATTGATCATCGGAACGGGTGGTGAGGCAGCTGCGGTCCAGCAGGCGCTTACCCACCCT
GTTCAGAGCGGCATGGAGATTGTGGGTTTCTATTCGCCGGAGAAGGAGGGCGAGGTCTGCGTTGATTCGGGGCGACTGCT
GCGGGGGAGCAACCTTCTGGACGTTGTCCGCCGCCACCGCGTTAGCGAGATCATCGTCGCGGTCAAGGAGCGTCGGGGGG
GCGTCCTGCCGCTGAGGGAACTGTTGGATTGCAAGGTGGCGGGCGTTAGGATTCTCGACCTTTCGTCCTTCTTCGAGCGG
GTGCAAGGGCAGGTTCGAATCGACTCGCTCCGGGCCAGTTGGCTTATCTACGGCGACGGTTTTCGGCAGGGTTTGGCCCG
TACGGTGGTCAAGCGTTTCTTTGACATCGTGGTGGCCGTCGTCCTGCTGGCGCTGGCGCTGCCGCTGATGCTCTGCACCG
CGATCCTGATTTTGCTGGAGGACGGCGCCCCGATCTTCTACCGACAGGAACGCGTGGGGCTCGGGGGGCGGGTTTTCAAG
GTCATCAAGTTCCGCAGCATGCGGCGGGACGCTGAAAAGGACGGTAAGCCGCGCTGGGCGAGTTCCAATGACGATCGCGC
GACGCGAATCGGCAGGATCATCCGTAAGCTCCGGATTGATGAGCTTCCTCAGCTGCTCAACGTCTTGGTTGGCGACATGA
GCCTGGTCGGGCCGCGTCCCGAGCGTCCTTTCTTTGTCGATCAGTTGGCGGCACAGATCCCGTTCTACGGTGTGCGCCAT
TGCGTAAAGCCGGGGGTGACCGGCTGGGCTCAGGTTCGCTATCAGTACGGCGCGTCGATCGACGACGCCGTGCAGAAGTT
GCAATACGACCTTTACTACGTGAAGAACCACACGCTGGTCTTGGATACTCTGGTGCTGTTTGAGACCGTGCGGGTAGTCC
TCACGGGTGAGGGCGCGCACTGA

Upstream 100 bases:

>100_bases
AGAGTTTAACGCTCGCTGTAACCCCGTTGGGGTAAGGTTGAAAGCCTCCCGGGGGAGCCTGACGCTCCTTCGGTAGTCCA
TCGGAGTATCGGGGATTCCC

Downstream 100 bases:

>100_bases
AGGCCGGAGGGCGGCCTTTCAGCGCCAACGATGCCGATCCAGCCGCGGTGGCGCCTTGGGCGCCGCTTGCGCTTTAGATG
AGGTTGTTGAGTGGACGTCG

Product: glycosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MLKVFSHYLPSHTVQQILFDALLLFVAVLLAVFMQLQTEIIEWRVLVPSAMFFALSMVVLNTAMGLYRSAVRESQRGAVA
RVVLSVVVSVPVAYGVFSVLPWAEPSNVSVQISVLVLLGFVLVMRGLVNRRQASALFAPRVLIIGTGGEAAAVQQALTHP
VQSGMEIVGFYSPEKEGEVCVDSGRLLRGSNLLDVVRRHRVSEIIVAVKERRGGVLPLRELLDCKVAGVRILDLSSFFER
VQGQVRIDSLRASWLIYGDGFRQGLARTVVKRFFDIVVAVVLLALALPLMLCTAILILLEDGAPIFYRQERVGLGGRVFK
VIKFRSMRRDAEKDGKPRWASSNDDRATRIGRIIRKLRIDELPQLLNVLVGDMSLVGPRPERPFFVDQLAAQIPFYGVRH
CVKPGVTGWAQVRYQYGASIDDAVQKLQYDLYYVKNHTLVLDTLVLFETVRVVLTGEGAH

Sequences:

>Translated_460_residues
MLKVFSHYLPSHTVQQILFDALLLFVAVLLAVFMQLQTEIIEWRVLVPSAMFFALSMVVLNTAMGLYRSAVRESQRGAVA
RVVLSVVVSVPVAYGVFSVLPWAEPSNVSVQISVLVLLGFVLVMRGLVNRRQASALFAPRVLIIGTGGEAAAVQQALTHP
VQSGMEIVGFYSPEKEGEVCVDSGRLLRGSNLLDVVRRHRVSEIIVAVKERRGGVLPLRELLDCKVAGVRILDLSSFFER
VQGQVRIDSLRASWLIYGDGFRQGLARTVVKRFFDIVVAVVLLALALPLMLCTAILILLEDGAPIFYRQERVGLGGRVFK
VIKFRSMRRDAEKDGKPRWASSNDDRATRIGRIIRKLRIDELPQLLNVLVGDMSLVGPRPERPFFVDQLAAQIPFYGVRH
CVKPGVTGWAQVRYQYGASIDDAVQKLQYDLYYVKNHTLVLDTLVLFETVRVVLTGEGAH
>Mature_460_residues
MLKVFSHYLPSHTVQQILFDALLLFVAVLLAVFMQLQTEIIEWRVLVPSAMFFALSMVVLNTAMGLYRSAVRESQRGAVA
RVVLSVVVSVPVAYGVFSVLPWAEPSNVSVQISVLVLLGFVLVMRGLVNRRQASALFAPRVLIIGTGGEAAAVQQALTHP
VQSGMEIVGFYSPEKEGEVCVDSGRLLRGSNLLDVVRRHRVSEIIVAVKERRGGVLPLRELLDCKVAGVRILDLSSFFER
VQGQVRIDSLRASWLIYGDGFRQGLARTVVKRFFDIVVAVVLLALALPLMLCTAILILLEDGAPIFYRQERVGLGGRVFK
VIKFRSMRRDAEKDGKPRWASSNDDRATRIGRIIRKLRIDELPQLLNVLVGDMSLVGPRPERPFFVDQLAAQIPFYGVRH
CVKPGVTGWAQVRYQYGASIDDAVQKLQYDLYYVKNHTLVLDTLVLFETVRVVLTGEGAH

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=322, Percent_Identity=31.6770186335404, Blast_Score=146, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 51220; Mature: 51220

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKVFSHYLPSHTVQQILFDALLLFVAVLLAVFMQLQTEIIEWRVLVPSAMFFALSMVVL
CCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NTAMGLYRSAVRESQRGAVARVVLSVVVSVPVAYGVFSVLPWAEPSNVSVQISVLVLLGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHH
VLVMRGLVNRRQASALFAPRVLIIGTGGEAAAVQQALTHPVQSGMEIVGFYSPEKEGEVC
HHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCEE
VDSGRLLRGSNLLDVVRRHRVSEIIVAVKERRGGVLPLRELLDCKVAGVRILDLSSFFER
ECCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHCCEEEEEHHHHHHH
VQGQVRIDSLRASWLIYGDGFRQGLARTVVKRFFDIVVAVVLLALALPLMLCTAILILLE
HCCCEEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DGAPIFYRQERVGLGGRVFKVIKFRSMRRDAEKDGKPRWASSNDDRATRIGRIIRKLRID
CCCCEEEEHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
ELPQLLNVLVGDMSLVGPRPERPFFVDQLAAQIPFYGVRHCVKPGVTGWAQVRYQYGASI
HHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHCCCH
DDAVQKLQYDLYYVKNHTLVLDTLVLFETVRVVLTGEGAH
HHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHEECCCCC
>Mature Secondary Structure
MLKVFSHYLPSHTVQQILFDALLLFVAVLLAVFMQLQTEIIEWRVLVPSAMFFALSMVVL
CCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NTAMGLYRSAVRESQRGAVARVVLSVVVSVPVAYGVFSVLPWAEPSNVSVQISVLVLLGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHH
VLVMRGLVNRRQASALFAPRVLIIGTGGEAAAVQQALTHPVQSGMEIVGFYSPEKEGEVC
HHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCEE
VDSGRLLRGSNLLDVVRRHRVSEIIVAVKERRGGVLPLRELLDCKVAGVRILDLSSFFER
ECCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHCCEEEEEHHHHHHH
VQGQVRIDSLRASWLIYGDGFRQGLARTVVKRFFDIVVAVVLLALALPLMLCTAILILLE
HCCCEEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DGAPIFYRQERVGLGGRVFKVIKFRSMRRDAEKDGKPRWASSNDDRATRIGRIIRKLRID
CCCCEEEEHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
ELPQLLNVLVGDMSLVGPRPERPFFVDQLAAQIPFYGVRHCVKPGVTGWAQVRYQYGASI
HHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHCCCH
DDAVQKLQYDLYYVKNHTLVLDTLVLFETVRVVLTGEGAH
HHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]