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The map label for this gene is colS [H]

Identifier: 119899449

GI number: 119899449

Start: 3467557

End: 3468843

Strand: Direct

Name: colS [H]

Synonym: azo3159

Alternate gene names: 119899449

Gene position: 3467557-3468843 (Clockwise)

Preceding gene: 119899448

Following gene: 119899450

Centisome position: 79.24

GC content: 69.23

Gene sequence:

>1287_bases
ATGAGGGTTGAGCGCAGCCTCGCGCGCCGCATCGTCGTCGCGTTCACGCTGATGACCTGCGTCGTCGCCGGGCTCTTTTC
CGCCGGTGTCGTCACCGCGATCAGCCTTGCCGAAGACCAGCTCGTCACCGAAGCGATGGACAGGCAGCTGGACTTCGTGA
TCGAGAGCGGTGAAGGCGCGGCGTCGCTGCGGCTCAGCGCCGGGCTCTACCTCTACCGCAGTGGCGCGCCGACCGATCCG
CCCCTGCCGGAATGGCTGCGGGGCCTGGAGCCGGGCTTTCACGAAGTCATCCGCGATGACGTCGAATACGAGACCCTGGT
GCGTGACCAGGACGGGCTGCGCTACGTACTGCTGCTCGACCAGGAAGATTTCGAAAGCCGCGAACGCACCACCTTCACCA
CCGTGTGGGCAGGCTTTGCGCTGAGCGTGGCGGCCGCCTTCGCGATCGGCGTGACCACCGCGCGCCGCGTCATTGCCCCA
GTGATCCGGCTCGCCGGACAGGTGCAGCACCGCGACCAGCTGCTGCCGCTCGCGCCGCCGCTCGCGCCCGACTACGCGGA
CGACGAGGTCGGGCAGCTTGCGCGCGCTTTCGACAACACGCTGGGCCAGCTGCGCCAGGCGCTGCAGCGCGAAACGCTGT
TCACCAGCGACGTCAGCCACGAGCTGCGCACGCCCTTGATGGCCATCGCCAGCACCTGCGAGCTGCTGCTCGAAGAACTG
CCGGCGGGTACCCGCGCCCACGAGCAGTTGCGCCGCCTGCAGCACGGTTGCGAGGAAATGCGCGAACTCGTCGAAACCTT
CCTGTGCCTTGCCCGCGGCAACGGCAGGAAAGGTGCGGGGGTCGCGCACGCCGGGCTGCGCACGATCGCTGAGGAGCAGG
CTGGGCGCTGGCAGGAGGAGGCGCGCTTGCGCGGCCTCACGCTGCGGCTCGAGGACCGGCTGACCACCGACGGCGCCTAT
CCCGCTCCCTTGCTGCGCGCGGTAATGTCCAACCTGCTGCGCAACGCACTGCACTACACCGACCAGGGCGGCGTCCGCCT
GATCCTCGAGGACGGCGCCTTCAGCGTGGTGGACAGCGGCGCCGGCATCCCGGAGTCTGAGCGGCTGAGCATCTTCCAGC
CCTTTGTCCGGGGCGGCCACAGTCGCGGCGACGGCATCGGACTCGGCCTGTCGCTGGTGCAGCGGATCTGCCAACAGCAG
GACTGGTCGATCACGCTCGCCGAAGCGGAGGGCGGCGGCTGTCACTTCCGGGTCGGTCTGGCCCCGCCCGGCCTCACGGA
AATTTGA

Upstream 100 bases:

>100_bases
CTGCGCAGCCACATCCACGCCCTGCGGCAACAGATCGACAAGCCCTTCCCGACCCCCCTGCTCCATACGGTGCATGGCGT
TGGCTTCCGCCTTGCCGCGC

Downstream 100 bases:

>100_bases
CACCCCGCTGACGGGCGGCTCACGCCGCCCGACCTAAGCTGCGGCTCCATGTCCCCTACGGAGCCCTCCATGCGCGCGTC
CGGCCCGCAGCCGATCCGGC

Product: two-component system sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MRVERSLARRIVVAFTLMTCVVAGLFSAGVVTAISLAEDQLVTEAMDRQLDFVIESGEGAASLRLSAGLYLYRSGAPTDP
PLPEWLRGLEPGFHEVIRDDVEYETLVRDQDGLRYVLLLDQEDFESRERTTFTTVWAGFALSVAAAFAIGVTTARRVIAP
VIRLAGQVQHRDQLLPLAPPLAPDYADDEVGQLARAFDNTLGQLRQALQRETLFTSDVSHELRTPLMAIASTCELLLEEL
PAGTRAHEQLRRLQHGCEEMRELVETFLCLARGNGRKGAGVAHAGLRTIAEEQAGRWQEEARLRGLTLRLEDRLTTDGAY
PAPLLRAVMSNLLRNALHYTDQGGVRLILEDGAFSVVDSGAGIPESERLSIFQPFVRGGHSRGDGIGLGLSLVQRICQQQ
DWSITLAEAEGGGCHFRVGLAPPGLTEI

Sequences:

>Translated_428_residues
MRVERSLARRIVVAFTLMTCVVAGLFSAGVVTAISLAEDQLVTEAMDRQLDFVIESGEGAASLRLSAGLYLYRSGAPTDP
PLPEWLRGLEPGFHEVIRDDVEYETLVRDQDGLRYVLLLDQEDFESRERTTFTTVWAGFALSVAAAFAIGVTTARRVIAP
VIRLAGQVQHRDQLLPLAPPLAPDYADDEVGQLARAFDNTLGQLRQALQRETLFTSDVSHELRTPLMAIASTCELLLEEL
PAGTRAHEQLRRLQHGCEEMRELVETFLCLARGNGRKGAGVAHAGLRTIAEEQAGRWQEEARLRGLTLRLEDRLTTDGAY
PAPLLRAVMSNLLRNALHYTDQGGVRLILEDGAFSVVDSGAGIPESERLSIFQPFVRGGHSRGDGIGLGLSLVQRICQQQ
DWSITLAEAEGGGCHFRVGLAPPGLTEI
>Mature_428_residues
MRVERSLARRIVVAFTLMTCVVAGLFSAGVVTAISLAEDQLVTEAMDRQLDFVIESGEGAASLRLSAGLYLYRSGAPTDP
PLPEWLRGLEPGFHEVIRDDVEYETLVRDQDGLRYVLLLDQEDFESRERTTFTTVWAGFALSVAAAFAIGVTTARRVIAP
VIRLAGQVQHRDQLLPLAPPLAPDYADDEVGQLARAFDNTLGQLRQALQRETLFTSDVSHELRTPLMAIASTCELLLEEL
PAGTRAHEQLRRLQHGCEEMRELVETFLCLARGNGRKGAGVAHAGLRTIAEEQAGRWQEEARLRGLTLRLEDRLTTDGAY
PAPLLRAVMSNLLRNALHYTDQGGVRLILEDGAFSVVDSGAGIPESERLSIFQPFVRGGHSRGDGIGLGLSLVQRICQQQ
DWSITLAEAEGGGCHFRVGLAPPGLTEI

Specific function: Member of the two-component regulatory system BasS/BasR Autophosphorylates and activates BasR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790551, Length=268, Percent_Identity=32.4626865671642, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789808, Length=255, Percent_Identity=29.8039215686275, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI87082128, Length=224, Percent_Identity=27.6785714285714, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081816, Length=234, Percent_Identity=30.7692307692308, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1790436, Length=216, Percent_Identity=30.5555555555556, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788393, Length=312, Percent_Identity=29.1666666666667, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786783, Length=283, Percent_Identity=27.208480565371, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1789403, Length=315, Percent_Identity=27.3015873015873, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1790861, Length=300, Percent_Identity=28.3333333333333, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789149, Length=243, Percent_Identity=25.5144032921811, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790346, Length=242, Percent_Identity=26.0330578512397, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1786912, Length=235, Percent_Identity=30.2127659574468, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 46843; Mature: 46843

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVERSLARRIVVAFTLMTCVVAGLFSAGVVTAISLAEDQLVTEAMDRQLDFVIESGEGA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCC
ASLRLSAGLYLYRSGAPTDPPLPEWLRGLEPGFHEVIRDDVEYETLVRDQDGLRYVLLLD
EEEEEECCEEEEECCCCCCCCHHHHHHHCCCCHHHHHHHCCHHHHHHCCCCCCEEEEEEC
QEDFESRERTTFTTVWAGFALSVAAAFAIGVTTARRVIAPVIRLAGQVQHRDQLLPLAPP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
LAPDYADDEVGQLARAFDNTLGQLRQALQRETLFTSDVSHELRTPLMAIASTCELLLEEL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PAGTRAHEQLRRLQHGCEEMRELVETFLCLARGNGRKGAGVAHAGLRTIAEEQAGRWQEE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHH
ARLRGLTLRLEDRLTTDGAYPAPLLRAVMSNLLRNALHYTDQGGVRLILEDGAFSVVDSG
HHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEECC
AGIPESERLSIFQPFVRGGHSRGDGIGLGLSLVQRICQQQDWSITLAEAEGGGCHFRVGL
CCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEC
APPGLTEI
CCCCCCCC
>Mature Secondary Structure
MRVERSLARRIVVAFTLMTCVVAGLFSAGVVTAISLAEDQLVTEAMDRQLDFVIESGEGA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCC
ASLRLSAGLYLYRSGAPTDPPLPEWLRGLEPGFHEVIRDDVEYETLVRDQDGLRYVLLLD
EEEEEECCEEEEECCCCCCCCHHHHHHHCCCCHHHHHHHCCHHHHHHCCCCCCEEEEEEC
QEDFESRERTTFTTVWAGFALSVAAAFAIGVTTARRVIAPVIRLAGQVQHRDQLLPLAPP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
LAPDYADDEVGQLARAFDNTLGQLRQALQRETLFTSDVSHELRTPLMAIASTCELLLEEL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PAGTRAHEQLRRLQHGCEEMRELVETFLCLARGNGRKGAGVAHAGLRTIAEEQAGRWQEE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHH
ARLRGLTLRLEDRLTTDGAYPAPLLRAVMSNLLRNALHYTDQGGVRLILEDGAFSVVDSG
HHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEECC
AGIPESERLSIFQPFVRGGHSRGDGIGLGLSLVQRICQQQDWSITLAEAEGGGCHFRVGL
CCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEC
APPGLTEI
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8282725; 7610040; 9278503 [H]