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The map label for this gene is pcnB [H]

Identifier: 119899430

GI number: 119899430

Start: 3448752

End: 3450110

Strand: Direct

Name: pcnB [H]

Synonym: azo3140

Alternate gene names: 119899430

Gene position: 3448752-3450110 (Clockwise)

Preceding gene: 119899429

Following gene: 119899431

Centisome position: 78.81

GC content: 69.39

Gene sequence:

>1359_bases
ATGATCCGCAAACTGCTGCGCAAGGTCTTTACCCGCCAGGCGCCGCCAGCCGCCCCCGAACCCGCCCTGATCCCGGTCGA
CCAGCACGGCATACGCCGCGAGCAGTTGTCGCCCGCCGCACGCAAGGTGTGTTCGGTCCTGCAGGAACACGGCTACAAAG
CCTTTGTCGTCGGCGGCGCGGTGCGCGACCTGCTGATCGGCCATCCGCCCAAGGACTACGACGTCGCCACCAGCGCCACG
CCGGAGGAAGTGCGGGCGCTATTCCGCCGCGCCCGCATCATCGGCCGCCGCTTCAAGATCGTGCACGTGATGAGCGGGCC
GGAGACGATCGAGGTTTCCACCTTCCGCGCGCTGCAGGACGCCGAATCGGCCGAAACCGACGAACACGGCCGCGTGCTGC
GCGACAACGTCTATGGCTCGCAGGCCGAAGATGCCACCCGGCGCGACTTCACCGTCAATGCGCTGTACTACGACCCGGGC
ACCGAAACCATCGTCGACTACCACCACGGCGTCGCCGACGTGCAGCAGAAAACCCTGCGCATGATCGGCGACCCGCGCAC
GCGCTACCGCGAAGACCCGGTGCGCATGCTGCGTGCGGTGCGGCTCGGCGCCAAACTCGGCCTCAGCATCGACCCCGCCG
CGCGTGGACCGATCCGCGAGATGGCGTCGCTGCTGGAAAACGTGCCGGCGGCGCGCCTGTTTGACGAGATGCTCAAGCTG
CTGCTGTCCGGCTACGCGGTGAAGTGCCTGCGCCAGTTGCGCGAGGAGGGCCTGCACCACGGCCTGCTGCCGCTGCTCGA
CGTCATCCTTGAACAACCGATGGGCGAGCGCTTCGTCTGGCTGTCGCTGGAAAACACCGACGAGCGGGTGCGCCAGGACA
AGCCGGTCTCGCCCGGCTTCCTGTTCGCCACGCTGCTGTGGCACGAAGTGCTGGCCAACTGGGAAACCAAGAAGCGCGCC
GGCGAGCACGCCCAGCCGGCGCTGTTCCAGGCGATGGACGATGTGCTCGACGCCCAGGCCGGCAAACTCGCGATCACGCG
CCGCATCGTCGGCGACATCAAGGACATCTGGGCGCTGCAGCCGCGCTTCGACAAGCGCAGCGGCAAGATGCCCTACCGCC
TGATCGAACAGCCGCGCTACCGCGCCGGGTGGGACTTCCTGCGCCTGCGCGCGCATTCGGGCGAAATCCCGCTGGACGTC
CCCGAGTGGTGGGACCGCTTCGCCCACGCCGGCCACGACGAGCGCGACGCCCTGCTGCGCGACGCGCCGCAGGCCGAGGC
ACCCGCCAAGAAGCGCCGCCGGCGGCGGCGCAAACCCGGCGGCGCCGACAGCGCCGCACTGCCCGCCGAAGCCTCGTGA

Upstream 100 bases:

>100_bases
GCTGCGCTGACGCCACACCCACGGACACCGCCGCGCCGCCGCGGCGCGCTCCCATCAGTTATGATTGCGGGCTCTTGACG
CCCTCCACACCGACCCGCCG

Downstream 100 bases:

>100_bases
TGACCGCCCCGACGCGCGCCTACGTGGCGTTCGGGGCCAACCTCGGCGATCCGGCTGCAGCCTTTGCCCTGGCACTAGAA
CGCATCGCCGCCCTGCCCGG

Product: poly(A) polymerase

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MIRKLLRKVFTRQAPPAAPEPALIPVDQHGIRREQLSPAARKVCSVLQEHGYKAFVVGGAVRDLLIGHPPKDYDVATSAT
PEEVRALFRRARIIGRRFKIVHVMSGPETIEVSTFRALQDAESAETDEHGRVLRDNVYGSQAEDATRRDFTVNALYYDPG
TETIVDYHHGVADVQQKTLRMIGDPRTRYREDPVRMLRAVRLGAKLGLSIDPAARGPIREMASLLENVPAARLFDEMLKL
LLSGYAVKCLRQLREEGLHHGLLPLLDVILEQPMGERFVWLSLENTDERVRQDKPVSPGFLFATLLWHEVLANWETKKRA
GEHAQPALFQAMDDVLDAQAGKLAITRRIVGDIKDIWALQPRFDKRSGKMPYRLIEQPRYRAGWDFLRLRAHSGEIPLDV
PEWWDRFAHAGHDERDALLRDAPQAEAPAKKRRRRRRKPGGADSAALPAEAS

Sequences:

>Translated_452_residues
MIRKLLRKVFTRQAPPAAPEPALIPVDQHGIRREQLSPAARKVCSVLQEHGYKAFVVGGAVRDLLIGHPPKDYDVATSAT
PEEVRALFRRARIIGRRFKIVHVMSGPETIEVSTFRALQDAESAETDEHGRVLRDNVYGSQAEDATRRDFTVNALYYDPG
TETIVDYHHGVADVQQKTLRMIGDPRTRYREDPVRMLRAVRLGAKLGLSIDPAARGPIREMASLLENVPAARLFDEMLKL
LLSGYAVKCLRQLREEGLHHGLLPLLDVILEQPMGERFVWLSLENTDERVRQDKPVSPGFLFATLLWHEVLANWETKKRA
GEHAQPALFQAMDDVLDAQAGKLAITRRIVGDIKDIWALQPRFDKRSGKMPYRLIEQPRYRAGWDFLRLRAHSGEIPLDV
PEWWDRFAHAGHDERDALLRDAPQAEAPAKKRRRRRRKPGGADSAALPAEAS
>Mature_452_residues
MIRKLLRKVFTRQAPPAAPEPALIPVDQHGIRREQLSPAARKVCSVLQEHGYKAFVVGGAVRDLLIGHPPKDYDVATSAT
PEEVRALFRRARIIGRRFKIVHVMSGPETIEVSTFRALQDAESAETDEHGRVLRDNVYGSQAEDATRRDFTVNALYYDPG
TETIVDYHHGVADVQQKTLRMIGDPRTRYREDPVRMLRAVRLGAKLGLSIDPAARGPIREMASLLENVPAARLFDEMLKL
LLSGYAVKCLRQLREEGLHHGLLPLLDVILEQPMGERFVWLSLENTDERVRQDKPVSPGFLFATLLWHEVLANWETKKRA
GEHAQPALFQAMDDVLDAQAGKLAITRRIVGDIKDIWALQPRFDKRSGKMPYRLIEQPRYRAGWDFLRLRAHSGEIPLDV
PEWWDRFAHAGHDERDALLRDAPQAEAPAKKRRRRRRKPGGADSAALPAEAS

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=222, Percent_Identity=28.8288288288288, Blast_Score=89, Evalue=6e-18,
Organism=Escherichia coli, GI87081691, Length=438, Percent_Identity=42.9223744292237, Blast_Score=348, Evalue=3e-97,
Organism=Escherichia coli, GI1789436, Length=228, Percent_Identity=32.4561403508772, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71995920, Length=248, Percent_Identity=26.6129032258064, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI281360112, Length=236, Percent_Identity=27.9661016949153, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI21357337, Length=236, Percent_Identity=27.9661016949153, Blast_Score=100, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 51200; Mature: 51200

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRKLLRKVFTRQAPPAAPEPALIPVDQHGIRREQLSPAARKVCSVLQEHGYKAFVVGGA
CHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCHHHCCHHHHHHHHHHHHCCCEEEEECHH
VRDLLIGHPPKDYDVATSATPEEVRALFRRARIIGRRFKIVHVMSGPETIEVSTFRALQD
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCEEEHHHHHHHHH
AESAETDEHGRVLRDNVYGSQAEDATRRDFTVNALYYDPGTETIVDYHHGVADVQQKTLR
HHHCCCCCCCCCHHHCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHCCHHHHHHHHHH
MIGDPRTRYREDPVRMLRAVRLGAKLGLSIDPAARGPIREMASLLENVPAARLFDEMLKL
HHCCCHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHH
LLSGYAVKCLRQLREEGLHHGLLPLLDVILEQPMGERFVWLSLENTDERVRQDKPVSPGF
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEEECCCHHHHHCCCCCCCHH
LFATLLWHEVLANWETKKRAGEHAQPALFQAMDDVLDAQAGKLAITRRIVGDIKDIWALQ
HHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
PRFDKRSGKMPYRLIEQPRYRAGWDFLRLRAHSGEIPLDVPEWWDRFAHAGHDERDALLR
CCCCCCCCCCCHHHHCCCCCCCCCHHHEEEECCCCCCCCCHHHHHHHHHCCCCHHHHHHH
DAPQAEAPAKKRRRRRRKPGGADSAALPAEAS
CCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIRKLLRKVFTRQAPPAAPEPALIPVDQHGIRREQLSPAARKVCSVLQEHGYKAFVVGGA
CHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCHHHCCHHHHHHHHHHHHCCCEEEEECHH
VRDLLIGHPPKDYDVATSATPEEVRALFRRARIIGRRFKIVHVMSGPETIEVSTFRALQD
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCEEEHHHHHHHHH
AESAETDEHGRVLRDNVYGSQAEDATRRDFTVNALYYDPGTETIVDYHHGVADVQQKTLR
HHHCCCCCCCCCHHHCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHCCHHHHHHHHHH
MIGDPRTRYREDPVRMLRAVRLGAKLGLSIDPAARGPIREMASLLENVPAARLFDEMLKL
HHCCCHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHH
LLSGYAVKCLRQLREEGLHHGLLPLLDVILEQPMGERFVWLSLENTDERVRQDKPVSPGF
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEEECCCHHHHHCCCCCCCHH
LFATLLWHEVLANWETKKRAGEHAQPALFQAMDDVLDAQAGKLAITRRIVGDIKDIWALQ
HHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
PRFDKRSGKMPYRLIEQPRYRAGWDFLRLRAHSGEIPLDVPEWWDRFAHAGHDERDALLR
CCCCCCCCCCCHHHHCCCCCCCCCHHHEEEECCCCCCCCCHHHHHHHHHCCCCHHHHHHH
DAPQAEAPAKKRRRRRRKPGGADSAALPAEAS
CCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]