Click here to switch to the map view.

The map label for this gene is miaA

Identifier: 119899421

GI number: 119899421

Start: 3438489

End: 3439451

Strand: Direct

Name: miaA

Synonym: azo3131

Alternate gene names: 119899421

Gene position: 3438489-3439451 (Clockwise)

Preceding gene: 119899419

Following gene: 119899422

Centisome position: 78.58

GC content: 71.55

Gene sequence:

>963_bases
GTGTCGAGCCCCGAATCCGCCCTGCCCCCCGCCCTGCTGCTGCTCGGCCCCACCGCCAGCGGCAAGACCGCCAGCGCGCT
CGCGCTCGCCGCCACGCTGCCGGTGGAGATCATCAGCGTGGATTCGGCCCTGGTGTACCGCGACATGGACATCGGCACCG
CCAAGCCGAGCGCCGCCGAACAGGCGGTCTGCCCGCATCACCTGATCGACGTCGTGAGCCCCGAAGAAGCGTATTCGGCA
GCCCGCTTCTGCGCGGAAGCAAGCGTGCTGATGCGCGACATCAGCGCGCGCGGCCGCATTCCGCTGCTGGCGGGCGGCAC
CATGCTGTACTTCAAGGCGCTGCGCGACGGCCTGTCCGACCTGCCGCCCGCCGACCCCGTGCTGCGCCGGGCCATCGAGG
AACGCGCCGCCGCAGCGGGCTGGCCGGCACTGCACGCCGAGCTTGCCCGGCTGGACCCGGACGCGGCCGCACGTCTCGAA
CCCACCGACGCCCAACGCATCCAGCGGGCGCTCGAAATCGTCACGCTCAGCGGCGCGCCGCTGGCAGCCAGTTACGCACG
CCGCGAAGATGCGCCCCTGCCCTGCCGGCTGCTGCCGATCGCGCTCGCACCGTCGGACCGTGCGGTGCTGCACGCCCGCA
TCGAACAGCGTTTCGACCAGATGCTGGCTGCGGGCCTGGTCGATGAAGTCGCCGCGCTGCGCGAGCGCTACGTCCTGCAG
CCGCAAATGGCCTCGATGCGCTGTGTCGGTTACCGGCAGGCGTGGGAATTCCTCGACGGCGAGATCGACCGCGCCACGCT
GCGCTTCAAGGGCATTGCCGCCACCCGCCAGCTGGCCAAGCGCCAGCTCACCTGGCAGCGCCAGTTCCGCGACCAATGGC
CGGAGCTTGTCGAACTCGACTGCCTCGCGCCCGATCTGCCGCAGCATGTGCGCGACACCGCCCTGCGCCTGCTCGGCGCC
TGA

Upstream 100 bases:

>100_bases
GCGACGACCATCCGCCAGCAAGCGCGCCGCCCCCGCACGCGGCACTCGCCGCCGCCTCGCTGTCGGCGGATAATGGCCGC
CCGCCTGCGCCTGCCCGATC

Downstream 100 bases:

>100_bases
CCTGCAACCCCTTCTTTTCCGGAACAACGACCATGGAAGAACAAGTCCGCGAAGACGTCCGCCAATGGCTGGAGAAGGTC
GTCATCGGCCTCAACCTCTG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MSSPESALPPALLLLGPTASGKTASALALAATLPVEIISVDSALVYRDMDIGTAKPSAAEQAVCPHHLIDVVSPEEAYSA
ARFCAEASVLMRDISARGRIPLLAGGTMLYFKALRDGLSDLPPADPVLRRAIEERAAAAGWPALHAELARLDPDAAARLE
PTDAQRIQRALEIVTLSGAPLAASYARREDAPLPCRLLPIALAPSDRAVLHARIEQRFDQMLAAGLVDEVAALRERYVLQ
PQMASMRCVGYRQAWEFLDGEIDRATLRFKGIAATRQLAKRQLTWQRQFRDQWPELVELDCLAPDLPQHVRDTALRLLGA

Sequences:

>Translated_320_residues
MSSPESALPPALLLLGPTASGKTASALALAATLPVEIISVDSALVYRDMDIGTAKPSAAEQAVCPHHLIDVVSPEEAYSA
ARFCAEASVLMRDISARGRIPLLAGGTMLYFKALRDGLSDLPPADPVLRRAIEERAAAAGWPALHAELARLDPDAAARLE
PTDAQRIQRALEIVTLSGAPLAASYARREDAPLPCRLLPIALAPSDRAVLHARIEQRFDQMLAAGLVDEVAALRERYVLQ
PQMASMRCVGYRQAWEFLDGEIDRATLRFKGIAATRQLAKRQLTWQRQFRDQWPELVELDCLAPDLPQHVRDTALRLLGA
>Mature_319_residues
SSPESALPPALLLLGPTASGKTASALALAATLPVEIISVDSALVYRDMDIGTAKPSAAEQAVCPHHLIDVVSPEEAYSAA
RFCAEASVLMRDISARGRIPLLAGGTMLYFKALRDGLSDLPPADPVLRRAIEERAAAAGWPALHAELARLDPDAAARLEP
TDAQRIQRALEIVTLSGAPLAASYARREDAPLPCRLLPIALAPSDRAVLHARIEQRFDQMLAAGLVDEVAALRERYVLQP
QMASMRCVGYRQAWEFLDGEIDRATLRFKGIAATRQLAKRQLTWQRQFRDQWPELVELDCLAPDLPQHVRDTALRLLGA

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=301, Percent_Identity=31.8936877076412, Blast_Score=142, Evalue=4e-34,
Organism=Escherichia coli, GI1790613, Length=316, Percent_Identity=52.5316455696203, Blast_Score=307, Evalue=7e-85,
Organism=Caenorhabditis elegans, GI25144712, Length=273, Percent_Identity=27.1062271062271, Blast_Score=105, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6324848, Length=287, Percent_Identity=29.2682926829268, Blast_Score=132, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24649830, Length=351, Percent_Identity=27.9202279202279, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_AZOSB (A1KA92)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_934634.1
- ProteinModelPortal:   A1KA92
- SMR:   A1KA92
- STRING:   A1KA92
- GeneID:   4608637
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo3131
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- PhylomeDB:   A1KA92
- ProtClustDB:   PRK00091
- BioCyc:   ASP62928:AZO3131-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34815; Mature: 34683

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPESALPPALLLLGPTASGKTASALALAATLPVEIISVDSALVYRDMDIGTAKPSAAE
CCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCEEEEECCCHHEEECCCCCCCCCCCHH
QAVCPHHLIDVVSPEEAYSAARFCAEASVLMRDISARGRIPLLAGGTMLYFKALRDGLSD
HHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHC
LPPADPVLRRAIEERAAAAGWPALHAELARLDPDAAARLEPTDAQRIQRALEIVTLSGAP
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHHEECCCC
LAASYARREDAPLPCRLLPIALAPSDRAVLHARIEQRFDQMLAAGLVDEVAALRERYVLQ
HHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PQMASMRCVGYRQAWEFLDGEIDRATLRFKGIAATRQLAKRQLTWQRQFRDQWPELVELD
CHHHHHHHHHHHHHHHHHCCCCCHHHHEEHHHHHHHHHHHHHHHHHHHHHHHCHHHEEHH
CLAPDLPQHVRDTALRLLGA
CCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SSPESALPPALLLLGPTASGKTASALALAATLPVEIISVDSALVYRDMDIGTAKPSAAE
CCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCEEEEECCCHHEEECCCCCCCCCCCHH
QAVCPHHLIDVVSPEEAYSAARFCAEASVLMRDISARGRIPLLAGGTMLYFKALRDGLSD
HHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHC
LPPADPVLRRAIEERAAAAGWPALHAELARLDPDAAARLEPTDAQRIQRALEIVTLSGAP
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHHEECCCC
LAASYARREDAPLPCRLLPIALAPSDRAVLHARIEQRFDQMLAAGLVDEVAALRERYVLQ
HHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PQMASMRCVGYRQAWEFLDGEIDRATLRFKGIAATRQLAKRQLTWQRQFRDQWPELVELD
CHHHHHHHHHHHHHHHHHCCCCCHHHHEEHHHHHHHHHHHHHHHHHHHHHHHCHHHEEHH
CLAPDLPQHVRDTALRLLGA
CCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA