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The map label for this gene is lysC [H]

Identifier: 119899405

GI number: 119899405

Start: 3423208

End: 3424434

Strand: Reverse

Name: lysC [H]

Synonym: azo3115

Alternate gene names: 119899405

Gene position: 3424434-3423208 (Counterclockwise)

Preceding gene: 119899407

Following gene: 119899404

Centisome position: 78.25

GC content: 63.9

Gene sequence:

>1227_bases
ATGGCACTGATAGTTCAGAAGTATGGCGGCACCTCGGTCGGCAGCCCTGAGCGCATCAAGAACGTCGCCAGCAAGGTGGC
GAAGTTCCGCGCGGAGGGCCACCAGGTGGTCGTCGTGGTATCGGCGATGAGCGGTGAAACAAACCGCCTGATCGCACTGG
CCAAGGATGTTGCGGCGAAGCCCGACCCGCGCGAACTCGACGTGGTCGTGTCCACCGGCGAGCAGGTCACGATCGGTCTG
CTGTGCATGGCGCTGCAGGACATCGGCGTGCCGGCGAAAAGCTACACCGGCGCTCAGGTGAAGATCCTGACCGACAGTGC
GCACACCAAGGCGCGCATCCTGAACATCGACGAAGCGCCGATCCGCAAGGACCTCGACGCCGGCGCGGTGGTCGTCGTGG
CCGGTTTCCAGGGCGTGGACGAACACGGCAACATCACCACGCTGGGCCGTGGCGGCTCCGACACCACCGGCGTCGCCCTC
GCCGCCGCACTCAAGGCGGACGAGTGCCAGATCTACACCGACGTCGACGGGGTCTACACCACCGATCCGCGCATCGTCCC
CGAAGCCCGCAAGCTCGACACCATCACCTTCGAGGAAATGCTGGAAATGGCCAGCCTCGGTTCGAAGGTGCTGCAGATCC
GCTCGGTCGAGTTCGCCGGCAAGTACAAGGTCAAGCTGCGCGTGCTGTCGAGCTTCCAGGAAGGAGGCCAAGGCACGCTC
ATCACCGTTGAGGAAGACAAGAACATGGAACAGCCCGTCATCTCCGGCATCGCCTTCAACCGCGACGAAGCCAAGGTCAC
CGTGCTCGGCGTGCCCGACAAGCCCGGCATCGCCTACCAGATCCTCGGCCCGGTCGCCGACGCCAACCTCGACGTCGACA
TGATCATCCAGAACGTCGGCCACGATGGCACCACCGACTTCTCGTTCACGCTCGCGCGCGGCGACCTGGAAAAGGCGGTC
GCGATCCTGGAAGGGGTCAAGACGCATATCGGCGCGCGCGCGATCGAAGGCGACAAGACCATGTGCAAGGTCTCCATCGT
TGGCGTCGGCATGCGGTCGCATCCGGGCGTGGCGTCCAAGATGTTCCGCACGCTGGCCGAAGAGGGCATCAACATCCAGA
TGATCTCGACCTCCGAAATCAAGATTTCCGTCGCGATCGACGAGAAGTATCTGGAACTGGCCGTGCGCGTGCTGCACAAG
GCATTCGGCCTCGACCAGCCCGTCTGA

Upstream 100 bases:

>100_bases
CCAAACTTTTCACATCGTGGACGCCGTGCGGTAGTCCTGCAAAAACCGGCTGCTTTCGCTAGAATCCCAGCCCTTTATCC
ACAAGCACTTGGATACGACG

Downstream 100 bases:

>100_bases
TGACGCTTGCGCATCGCGCGCAAGCTTAAATTGACCATGGATGGCGGAATCTATATAGTGCCGCCTTCGTGTTGGAGACG
TGGCCGAGAGGTCGAAGGCA

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MALIVQKYGGTSVGSPERIKNVASKVAKFRAEGHQVVVVVSAMSGETNRLIALAKDVAAKPDPRELDVVVSTGEQVTIGL
LCMALQDIGVPAKSYTGAQVKILTDSAHTKARILNIDEAPIRKDLDAGAVVVVAGFQGVDEHGNITTLGRGGSDTTGVAL
AAALKADECQIYTDVDGVYTTDPRIVPEARKLDTITFEEMLEMASLGSKVLQIRSVEFAGKYKVKLRVLSSFQEGGQGTL
ITVEEDKNMEQPVISGIAFNRDEAKVTVLGVPDKPGIAYQILGPVADANLDVDMIIQNVGHDGTTDFSFTLARGDLEKAV
AILEGVKTHIGARAIEGDKTMCKVSIVGVGMRSHPGVASKMFRTLAEEGINIQMISTSEIKISVAIDEKYLELAVRVLHK
AFGLDQPV

Sequences:

>Translated_408_residues
MALIVQKYGGTSVGSPERIKNVASKVAKFRAEGHQVVVVVSAMSGETNRLIALAKDVAAKPDPRELDVVVSTGEQVTIGL
LCMALQDIGVPAKSYTGAQVKILTDSAHTKARILNIDEAPIRKDLDAGAVVVVAGFQGVDEHGNITTLGRGGSDTTGVAL
AAALKADECQIYTDVDGVYTTDPRIVPEARKLDTITFEEMLEMASLGSKVLQIRSVEFAGKYKVKLRVLSSFQEGGQGTL
ITVEEDKNMEQPVISGIAFNRDEAKVTVLGVPDKPGIAYQILGPVADANLDVDMIIQNVGHDGTTDFSFTLARGDLEKAV
AILEGVKTHIGARAIEGDKTMCKVSIVGVGMRSHPGVASKMFRTLAEEGINIQMISTSEIKISVAIDEKYLELAVRVLHK
AFGLDQPV
>Mature_407_residues
ALIVQKYGGTSVGSPERIKNVASKVAKFRAEGHQVVVVVSAMSGETNRLIALAKDVAAKPDPRELDVVVSTGEQVTIGLL
CMALQDIGVPAKSYTGAQVKILTDSAHTKARILNIDEAPIRKDLDAGAVVVVAGFQGVDEHGNITTLGRGGSDTTGVALA
AALKADECQIYTDVDGVYTTDPRIVPEARKLDTITFEEMLEMASLGSKVLQIRSVEFAGKYKVKLRVLSSFQEGGQGTLI
TVEEDKNMEQPVISGIAFNRDEAKVTVLGVPDKPGIAYQILGPVADANLDVDMIIQNVGHDGTTDFSFTLARGDLEKAVA
ILEGVKTHIGARAIEGDKTMCKVSIVGVGMRSHPGVASKMFRTLAEEGINIQMISTSEIKISVAIDEKYLELAVRVLHKA
FGLDQPV

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=289, Percent_Identity=33.9100346020761, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1790455, Length=428, Percent_Identity=26.4018691588785, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1790376, Length=170, Percent_Identity=28.8235294117647, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320893, Length=367, Percent_Identity=28.8828337874659, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 43439; Mature: 43307

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALIVQKYGGTSVGSPERIKNVASKVAKFRAEGHQVVVVVSAMSGETNRLIALAKDVAAK
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEEEEEHHHCCC
PDPRELDVVVSTGEQVTIGLLCMALQDIGVPAKSYTGAQVKILTDSAHTKARILNIDEAP
CCCCEEEEEEECCCEEHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCC
IRKDLDAGAVVVVAGFQGVDEHGNITTLGRGGSDTTGVALAAALKADECQIYTDVDGVYT
CCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCCHHHHEEEECCCCEEEEECCCCEEE
TDPRIVPEARKLDTITFEEMLEMASLGSKVLQIRSVEFAGKYKVKLRVLSSFQEGGQGTL
CCCCCCCCCHHCCEECHHHHHHHHHCCHHEEEEEEEEECCEEEEEEEEEHHHHCCCCEEE
ITVEEDKNMEQPVISGIAFNRDEAKVTVLGVPDKPGIAYQILGPVADANLDVDMIIQNVG
EEEECCCCCCCHHHHCEEECCCCCEEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHCC
HDGTTDFSFTLARGDLEKAVAILEGVKTHIGARAIEGDKTMCKVSIVGVGMRSHPGVASK
CCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCEEECCCCCEEEEEEEEECCCCCCCHHHH
MFRTLAEEGINIQMISTSEIKISVAIDEKYLELAVRVLHKAFGLDQPV
HHHHHHHCCCEEEEEECCEEEEEEEECHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ALIVQKYGGTSVGSPERIKNVASKVAKFRAEGHQVVVVVSAMSGETNRLIALAKDVAAK
EEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEEEEEHHHCCC
PDPRELDVVVSTGEQVTIGLLCMALQDIGVPAKSYTGAQVKILTDSAHTKARILNIDEAP
CCCCEEEEEEECCCEEHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCC
IRKDLDAGAVVVVAGFQGVDEHGNITTLGRGGSDTTGVALAAALKADECQIYTDVDGVYT
CCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCCHHHHEEEECCCCEEEEECCCCEEE
TDPRIVPEARKLDTITFEEMLEMASLGSKVLQIRSVEFAGKYKVKLRVLSSFQEGGQGTL
CCCCCCCCCHHCCEECHHHHHHHHHCCHHEEEEEEEEECCEEEEEEEEEHHHHCCCCEEE
ITVEEDKNMEQPVISGIAFNRDEAKVTVLGVPDKPGIAYQILGPVADANLDVDMIIQNVG
EEEECCCCCCCHHHHCEEECCCCCEEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHCC
HDGTTDFSFTLARGDLEKAVAILEGVKTHIGARAIEGDKTMCKVSIVGVGMRSHPGVASK
CCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCEEECCCCCEEEEEEEEECCCCCCCHHHH
MFRTLAEEGINIQMISTSEIKISVAIDEKYLELAVRVLHKAFGLDQPV
HHHHHHHCCCEEEEEECCEEEEEEEECHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]