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The map label for this gene is cooF [H]

Identifier: 119899221

GI number: 119899221

Start: 3216368

End: 3216844

Strand: Reverse

Name: cooF [H]

Synonym: azo2931

Alternate gene names: 119899221

Gene position: 3216844-3216368 (Counterclockwise)

Preceding gene: 119899222

Following gene: 119899220

Centisome position: 73.51

GC content: 63.94

Gene sequence:

>477_bases
ATGTGGAAATCACTGCACATTGACCCTGCCAAGTGCACCGGCTGCCTCCAATGCGAGATGGCCTGTTCCTACGAGCACAC
CGGGGTCATCAATCCGAGCAAGTCGCGGATCAAGGTCTTCAGCTTCGAGCACGAAGGGCGCAAGGTGCCTTACACGTGCA
CCCAGTGCACCGAGGCGTGGTGCATGCACTCCTGCCCGGTCGACGCAATCCGCCTCGACCTGACGACCGGCGCCAAGATG
GTGTTCGACGACACCTGTGTCGGCTGCAAGGTCTGCACGATCTCCTGTCCGTTCGGCACGATCAACTACAACCAGGCCAC
CGGCAAGGTGCAGAAGTGCGACCTGTGCGAGGGCGACCCCGCTTGTGCCAAGGCCTGCCCCACCGGTGCCATCACCTACG
TGGATGCCGACTGGACCGGGCTGGACAAGATGCGCGCCTGGGCCGCCAAGGCCAACACCCCCGCCACCGCCGCCTGA

Upstream 100 bases:

>100_bases
CGCAGGCCGGCCCTTGGAGATGAAGTTCGCAGACCGGTATGAAGTGTGCTTCATAGGTGCTGCTACACCAACGCAACAAT
GAAAGGACGAGGAGAATTCG

Downstream 100 bases:

>100_bases
GGAGAGCAATATGGGCTGGAACCGTAAAGTCCTGCGGGTGAACCTGACCGCGGGCACCTGCACCGAAGAACCGCTGAACA
TGGAATGGGCGCAGGAATAC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MWKSLHIDPAKCTGCLQCEMACSYEHTGVINPSKSRIKVFSFEHEGRKVPYTCTQCTEAWCMHSCPVDAIRLDLTTGAKM
VFDDTCVGCKVCTISCPFGTINYNQATGKVQKCDLCEGDPACAKACPTGAITYVDADWTGLDKMRAWAAKANTPATAA

Sequences:

>Translated_158_residues
MWKSLHIDPAKCTGCLQCEMACSYEHTGVINPSKSRIKVFSFEHEGRKVPYTCTQCTEAWCMHSCPVDAIRLDLTTGAKM
VFDDTCVGCKVCTISCPFGTINYNQATGKVQKCDLCEGDPACAKACPTGAITYVDADWTGLDKMRAWAAKANTPATAA
>Mature_158_residues
MWKSLHIDPAKCTGCLQCEMACSYEHTGVINPSKSRIKVFSFEHEGRKVPYTCTQCTEAWCMHSCPVDAIRLDLTTGAKM
VFDDTCVGCKVCTISCPFGTINYNQATGKVQKCDLCEGDPACAKACPTGAITYVDADWTGLDKMRAWAAKANTPATAA

Specific function: The carbon monoxide dehydrogenase (CODH) oxidizes carbon monoxide coupled, via CooF, to the reduction of a hydrogen cation by a hydrogenase (probably CooH). CooF is required in stoichiometric amounts in vitro for anchoring CODH to the membrane as well as

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367245, Length=155, Percent_Identity=32.9032258064516, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI87082179, Length=169, Percent_Identity=31.9526627218935, Blast_Score=81, Evalue=3e-17,
Organism=Escherichia coli, GI1788811, Length=159, Percent_Identity=33.3333333333333, Blast_Score=78, Evalue=2e-16,
Organism=Escherichia coli, GI87082180, Length=142, Percent_Identity=33.0985915492958, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1787963, Length=140, Percent_Identity=34.2857142857143, Blast_Score=74, Evalue=4e-15,
Organism=Escherichia coli, GI1789067, Length=160, Percent_Identity=30.625, Blast_Score=74, Evalue=5e-15,
Organism=Escherichia coli, GI1789079, Length=173, Percent_Identity=32.3699421965318, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1787122, Length=162, Percent_Identity=29.0123456790123, Blast_Score=70, Evalue=6e-14,
Organism=Escherichia coli, GI1787872, Length=150, Percent_Identity=29.3333333333333, Blast_Score=67, Evalue=4e-13,
Organism=Escherichia coli, GI2367345, Length=144, Percent_Identity=31.25, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1790326, Length=153, Percent_Identity=27.4509803921569, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1787749, Length=153, Percent_Identity=27.4509803921569, Blast_Score=64, Evalue=4e-12,
Organism=Escherichia coli, GI1789370, Length=93, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=5e-12,
Organism=Escherichia coli, GI87082114, Length=177, Percent_Identity=28.2485875706215, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 17134; Mature: 17134

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

10.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
13.3 %Cys+Met (Translated Protein)
10.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
13.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWKSLHIDPAKCTGCLQCEMACSYEHTGVINPSKSRIKVFSFEHEGRKVPYTCTQCTEAW
CCCCCCCCHHHCCEEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCEEHHHHHHHH
CMHSCPVDAIRLDLTTGAKMVFDDTCVGCKVCTISCPFGTINYNQATGKVQKCDLCEGDP
HHCCCCCCEEEEEECCCCEEEEECCCCCEEEEEEECCCCCEECCCCCCCEEECCCCCCCC
ACAKACPTGAITYVDADWTGLDKMRAWAAKANTPATAA
HHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MWKSLHIDPAKCTGCLQCEMACSYEHTGVINPSKSRIKVFSFEHEGRKVPYTCTQCTEAW
CCCCCCCCHHHCCEEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCEEHHHHHHHH
CMHSCPVDAIRLDLTTGAKMVFDDTCVGCKVCTISCPFGTINYNQATGKVQKCDLCEGDP
HHCCCCCCEEEEEECCCCEEEEECCCCCEEEEEEECCCCCEECCCCCCCEEECCCCCCCC
ACAKACPTGAITYVDADWTGLDKMRAWAAKANTPATAA
HHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1644755 [H]