Click here to switch to the map view.

The map label for this gene is nirB [C]

Identifier: 119899219

GI number: 119899219

Start: 3213150

End: 3214418

Strand: Reverse

Name: nirB [C]

Synonym: azo2929

Alternate gene names: 119899219

Gene position: 3214418-3213150 (Counterclockwise)

Preceding gene: 119899220

Following gene: 119899218

Centisome position: 73.45

GC content: 69.98

Gene sequence:

>1269_bases
ATGAAACACCTGATACTCGGCAACGGCCCGGCCGGCGTGGTCGCGGCCGAAACCCTGCGCCGTGCCGCGCCGCAGGACGA
AATCGTCATGGTCGGCAGCGAGGATGCCCCGCCGTACTCGCGCATGGCCATTCCCTACCTGCTCGAAGAGAACATCGACG
AACGCGGCACCTGGCTGCGCAAGGACCCCGGCCATTTCGACCGCCTGCGCATCCGCGAGCTGCGCGGGCGCGCGGTGTCG
CTCGACACCGACCAGCGGCGCGTGCTGTTCGCCGACGGCCATTTCGAAGACTACGACCGCCTGCTGATCGCCACCGGCTC
GCATCCGGTCCGCCCGCCGATTCCCGGCGTCGATCTGCCCGAAGTGCAGACCTGCTGGACGCTGGCGGACGCGCGCGCGA
TCGCGGCCCACGCCCGTCCTGGCGCGCGCGTGCTGCAACTCGGCGCCGGCTTCATCGGCTGCATCATCATGGAAGCGCTC
GCCAAGCGCGGCGTGGAGCTGACCGTCGTGGAGATGGGCGACCGCATGGTGCCGCGCATGATGACGCCGCAGGCCGGCGG
CATGATCAAGCGCTGGGTCGAGAACAAGGGCATCCGCGTCGTCACCAACGCGGGCGTCAGCCGCATCGACCGCGGTACGG
ACGGCGCGCCGCTGCAGGTCACGCTGACCACCGGTGCCACGCTGGCGTGCGACCTCGTCATTGTCGCCGCGGGCGTCGCG
CCCAACGTGGCCTTCCTCGAAGGCACCGGCGTGCACGTGGCCAAGGGTGTGCTGGTGGATGCGCGGCTCGAAACCAGCGT
GCCCGGTATCTTCTCGGCTGGCGACGTCGCCGAGGCGCCGGACCTCTTCACCGGCGCCCACCTGGTAGCCGCGATCCAGC
CCAATGCGGCCGACCAGGCGCGCGTCGCAGCGCTCAACATGGCGGGCCGCGAAACCACGCTCAACGGCGTGCTCGCGATC
AACGTGCTCGACACGCTCGGGCTGATCTCGACCTCGTTCGGCCAGTGGTGGGGCGAAGCCGCGGACAAGGGCGGCAGCGG
GGTGGAGCATGTGGACGAGGCGCGCAGCCGCTACCTCAGCCTGCAGTTCAAGGACGACGTGATGATCGGCGCCACCTCGA
TCGGGCTGACCGAGCACGTGGGCGCGCTGCGCGGCCTGATCCACGGCCGCGTGCAACTGGGCGAGTGGAAGGCGCGGTTG
CTGCAGAACCCGCTGGCCTTCGTCGATGCCTACATCGCACGCTCGCAGCCGGTGGCACTGACGCATTGA

Upstream 100 bases:

>100_bases
GGGCTGTAAGGGCGCGCGGACGGGCCGCTGCGCCCGTCCGCGCCGACGATCGCCGGTCGCGCCCGCAAGGGCGCGGCCGG
CCTGCCATCGAGGACTAGCA

Downstream 100 bases:

>100_bases
GGGCGCGGTCGTGAAAATCGCGTTCAAGCTCTTTGCCTCGCTGGCGGACTACCTGCCTGCGGAGCGCAAGGGCAACCGTG
TCGATCTCGAGGTGTCGTCC

Product: putative oxidoreductase

Products: NA

Alternate protein names: NOXase [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MKHLILGNGPAGVVAAETLRRAAPQDEIVMVGSEDAPPYSRMAIPYLLEENIDERGTWLRKDPGHFDRLRIRELRGRAVS
LDTDQRRVLFADGHFEDYDRLLIATGSHPVRPPIPGVDLPEVQTCWTLADARAIAAHARPGARVLQLGAGFIGCIIMEAL
AKRGVELTVVEMGDRMVPRMMTPQAGGMIKRWVENKGIRVVTNAGVSRIDRGTDGAPLQVTLTTGATLACDLVIVAAGVA
PNVAFLEGTGVHVAKGVLVDARLETSVPGIFSAGDVAEAPDLFTGAHLVAAIQPNAADQARVAALNMAGRETTLNGVLAI
NVLDTLGLISTSFGQWWGEAADKGGSGVEHVDEARSRYLSLQFKDDVMIGATSIGLTEHVGALRGLIHGRVQLGEWKARL
LQNPLAFVDAYIARSQPVALTH

Sequences:

>Translated_422_residues
MKHLILGNGPAGVVAAETLRRAAPQDEIVMVGSEDAPPYSRMAIPYLLEENIDERGTWLRKDPGHFDRLRIRELRGRAVS
LDTDQRRVLFADGHFEDYDRLLIATGSHPVRPPIPGVDLPEVQTCWTLADARAIAAHARPGARVLQLGAGFIGCIIMEAL
AKRGVELTVVEMGDRMVPRMMTPQAGGMIKRWVENKGIRVVTNAGVSRIDRGTDGAPLQVTLTTGATLACDLVIVAAGVA
PNVAFLEGTGVHVAKGVLVDARLETSVPGIFSAGDVAEAPDLFTGAHLVAAIQPNAADQARVAALNMAGRETTLNGVLAI
NVLDTLGLISTSFGQWWGEAADKGGSGVEHVDEARSRYLSLQFKDDVMIGATSIGLTEHVGALRGLIHGRVQLGEWKARL
LQNPLAFVDAYIARSQPVALTH
>Mature_422_residues
MKHLILGNGPAGVVAAETLRRAAPQDEIVMVGSEDAPPYSRMAIPYLLEENIDERGTWLRKDPGHFDRLRIRELRGRAVS
LDTDQRRVLFADGHFEDYDRLLIATGSHPVRPPIPGVDLPEVQTCWTLADARAIAAHARPGARVLQLGAGFIGCIIMEAL
AKRGVELTVVEMGDRMVPRMMTPQAGGMIKRWVENKGIRVVTNAGVSRIDRGTDGAPLQVTLTTGATLACDLVIVAAGVA
PNVAFLEGTGVHVAKGVLVDARLETSVPGIFSAGDVAEAPDLFTGAHLVAAIQPNAADQARVAALNMAGRETTLNGVLAI
NVLDTLGLISTSFGQWWGEAADKGGSGVEHVDEARSRYLSLQFKDDVMIGATSIGLTEHVGALRGLIHGRVQLGEWKARL
LQNPLAFVDAYIARSQPVALTH

Specific function: Catalyzes the four-electron reduction of molecular oxygen to water [H]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=319, Percent_Identity=30.4075235109718, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI226437568, Length=319, Percent_Identity=30.4075235109718, Blast_Score=129, Evalue=7e-30,
Organism=Homo sapiens, GI65787454, Length=319, Percent_Identity=30.4075235109718, Blast_Score=129, Evalue=8e-30,
Organism=Escherichia coli, GI1789765, Length=398, Percent_Identity=26.1306532663317, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1789065, Length=314, Percent_Identity=24.203821656051, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788892, Length=283, Percent_Identity=26.8551236749117, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17559934, Length=284, Percent_Identity=29.5774647887324, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24639257, Length=324, Percent_Identity=31.7901234567901, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI281359715, Length=324, Percent_Identity=31.7901234567901, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI281359713, Length=324, Percent_Identity=31.7901234567901, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24639250, Length=324, Percent_Identity=31.7901234567901, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI18543267, Length=324, Percent_Identity=31.7901234567901, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24639252, Length=324, Percent_Identity=31.7901234567901, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24585130, Length=322, Percent_Identity=28.2608695652174, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.6.99.3 [H]

Molecular weight: Translated: 45099; Mature: 45099

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHLILGNGPAGVVAAETLRRAAPQDEIVMVGSEDAPPYSRMAIPYLLEENIDERGTWLR
CCEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHCCHHHHHCCCCCCCCCCC
KDPGHFDRLRIRELRGRAVSLDTDQRRVLFADGHFEDYDRLLIATGSHPVRPPIPGVDLP
CCCCCHHHHHHHHHCCCEEEECCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCC
EVQTCWTLADARAIAAHARPGARVLQLGAGFIGCIIMEALAKRGVELTVVEMGDRMVPRM
CHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHH
MTPQAGGMIKRWVENKGIRVVTNAGVSRIDRGTDGAPLQVTLTTGATLACDLVIVAAGVA
CCCCCCHHHHHHHHCCCEEEEECCCHHHHCCCCCCCCEEEEEECCCHHHHEEEHEECCCC
PNVAFLEGTGVHVAKGVLVDARLETSVPGIFSAGDVAEAPDLFTGAHLVAAIQPNAADQA
CCEEEEECCCCEEECCEEEEEEECCCCCCCCCCCCCCCCCCHHCCCEEEEEECCCCCCCH
RVAALNMAGRETTLNGVLAINVLDTLGLISTSFGQWWGEAADKGGSGVEHVDEARSRYLS
HEEEEECCCCCEECCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEEE
LQFKDDVMIGATSIGLTEHVGALRGLIHGRVQLGEWKARLLQNPLAFVDAYIARSQPVAL
EEECCCEEEEEECCCHHHHHHHHHHHHHCCEEEHHHHHHHHHCHHHHHHHHHHCCCCEEE
TH
CC
>Mature Secondary Structure
MKHLILGNGPAGVVAAETLRRAAPQDEIVMVGSEDAPPYSRMAIPYLLEENIDERGTWLR
CCEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHCCHHHHHCCCCCCCCCCC
KDPGHFDRLRIRELRGRAVSLDTDQRRVLFADGHFEDYDRLLIATGSHPVRPPIPGVDLP
CCCCCHHHHHHHHHCCCEEEECCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCC
EVQTCWTLADARAIAAHARPGARVLQLGAGFIGCIIMEALAKRGVELTVVEMGDRMVPRM
CHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHH
MTPQAGGMIKRWVENKGIRVVTNAGVSRIDRGTDGAPLQVTLTTGATLACDLVIVAAGVA
CCCCCCHHHHHHHHCCCEEEEECCCHHHHCCCCCCCCEEEEEECCCHHHHEEEHEECCCC
PNVAFLEGTGVHVAKGVLVDARLETSVPGIFSAGDVAEAPDLFTGAHLVAAIQPNAADQA
CCEEEEECCCCEEECCEEEEEEECCCCCCCCCCCCCCCCCCHHCCCEEEEEECCCCCCCH
RVAALNMAGRETTLNGVLAINVLDTLGLISTSFGQWWGEAADKGGSGVEHVDEARSRYLS
HEEEEECCCCCEECCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEEE
LQFKDDVMIGATSIGLTEHVGALRGLIHGRVQLGEWKARLLQNPLAFVDAYIARSQPVAL
EEECCCEEEEEECCCHHHHHHHHHHHHHCCEEEHHHHHHHHHCHHHHHHHHHHCCCCEEE
TH
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]