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The map label for this gene is oprM3 [H]

Identifier: 119899202

GI number: 119899202

Start: 3197732

End: 3199141

Strand: Reverse

Name: oprM3 [H]

Synonym: azo2912

Alternate gene names: 119899202

Gene position: 3199141-3197732 (Counterclockwise)

Preceding gene: 119899209

Following gene: 119899201

Centisome position: 73.11

GC content: 71.7

Gene sequence:

>1410_bases
TTGCGTCCGGTGGTCTTCGGTCTCGCGCTGACAGGCGTTGCTGCCTGCTCGCTTGCGCCGCCCTATGAGCTTCCGGCCCT
GCCGGTGGAAAGCCGCTATCCGGCCGACGCCCCGGTGGCGCCGGCCGCGCAGCGTGCGGCCGACACCAGCTGGCAGGACT
ACTTCGCCGACCCTGCGCTACGCCGCCTGATCGGGCAGGCGCTGGATAACAGCCGTGATCTGCGCGCCGCGGTGCTGCGG
GTCGAGGAGGCGCGTGCCGCCTTCGGCATCCGGCGTGCCGACCGCTTTCCCACCGTGGGGCTCGGTTTCGAAGGCGTGCG
CGGGCGCACGCCAGCCGACCTCAGCATCACCGGCGAGGCGGTGACCAGCGGCCAGTACCAGATGGGCATCAGCCTGGCGA
GTTGGGAACTCGACCTGTGGGGGCGGGTGCGCAGTCTCGAAGGCGCGGCGCTGGAGGAGTTCCTTGCCACCGAGGCGGCG
CGTCGCGCTGTCACCGTCAGCGTGGTGGCCCAGGTTGCCGATGCCTACCTCACGCTGCGCGAACTGGACGAGCGCATCGT
GCTGGCGCAGCGCACCATCGACACCCGCCAGGAATCGTTCCGCATCTTCAGCCGCCGCGTGGAGGTCGGGGCGACCTCTC
GACTGGATCTCGCCCAGGTCGAAACGCTGCTGACGCAGGCCCAGGCGCTGGGCGCGCAGCTCGAACAGGCGCGCGCGCTG
CAGGCACATGCGCTGGCGGTGCTGGTGGGGGCGCCGCTCGACCTGCCCGCCGCGGCAACCCGCCTCGACGACGACCTCAT
CCTCCACGAACTCGATGCGGGGCTGCCGTCCGAACTGCTGACGGGACGCCCGGACATCGTCGCCGCCGAGCACCGCCTGA
AGGCGGCCCACGCCAGTATCGGCGCGGCGCGGGCTGCGTTTTTCCCTCGCATCGCGTTGACCGCGTCGGCCGGCAGCGCG
AGCGCCGCGCTCGACGGGCTGTTCGAACCCGGCAGCGGCGCATGGCGCTTCGCGCCGACGATCTCGCTGCCGATCTTCGA
TGCCGGCCGCAACCGCGCCGGGCTCGATCTCGCCGAGGTGCGGCGCGATCTTGCCGTCGCCAGTTACGAGAAGACGGTGC
AGCAGGCCTTCCGCGAGGTGGCCGACGCGCTTGCCGCGCGGCACTGGCTGGAGGCGCAGCTCGGCATCCAGCGTGCTGCG
CTCGCCACCCAGGTCGAACGCGCGCGCCTCGCCAAGTTGCGCTATGACAACGGCGCGGCGCGTTATCTCGAAGTGCTCGA
CGCCCAGCGCGACCTGCTTGCCGCGGAGCAGCAGGTGGTGCAGACCCGTCGCGCGCTGCTGTCCAGCCGGGTCGCGTTGT
ATGCAGCACTGGGCGGCGGCGCCCAGGAGCCGGAACGGACGCCCAGATAA

Upstream 100 bases:

>100_bases
CCCAACAGGTGTTGGATAATTCAGGCGTACTGTCGATCGCAAGTGGTAGCGTCCCATGAGCGTATGCGGATACCCGCGGT
GGAGCAGGTTGCGGCCCTGG

Downstream 100 bases:

>100_bases
GGAGCTGTGCGGATCATGAAATCTGCATTCAGGAAAACGTGGATTGTCCCGGCAGCGCTGGCGGCGGTGGCGGTGGTCGG
CGTGGTGACCTGGTATTCGA

Product: outer membrane efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MRPVVFGLALTGVAACSLAPPYELPALPVESRYPADAPVAPAAQRAADTSWQDYFADPALRRLIGQALDNSRDLRAAVLR
VEEARAAFGIRRADRFPTVGLGFEGVRGRTPADLSITGEAVTSGQYQMGISLASWELDLWGRVRSLEGAALEEFLATEAA
RRAVTVSVVAQVADAYLTLRELDERIVLAQRTIDTRQESFRIFSRRVEVGATSRLDLAQVETLLTQAQALGAQLEQARAL
QAHALAVLVGAPLDLPAAATRLDDDLILHELDAGLPSELLTGRPDIVAAEHRLKAAHASIGAARAAFFPRIALTASAGSA
SAALDGLFEPGSGAWRFAPTISLPIFDAGRNRAGLDLAEVRRDLAVASYEKTVQQAFREVADALAARHWLEAQLGIQRAA
LATQVERARLAKLRYDNGAARYLEVLDAQRDLLAAEQQVVQTRRALLSSRVALYAALGGGAQEPERTPR

Sequences:

>Translated_469_residues
MRPVVFGLALTGVAACSLAPPYELPALPVESRYPADAPVAPAAQRAADTSWQDYFADPALRRLIGQALDNSRDLRAAVLR
VEEARAAFGIRRADRFPTVGLGFEGVRGRTPADLSITGEAVTSGQYQMGISLASWELDLWGRVRSLEGAALEEFLATEAA
RRAVTVSVVAQVADAYLTLRELDERIVLAQRTIDTRQESFRIFSRRVEVGATSRLDLAQVETLLTQAQALGAQLEQARAL
QAHALAVLVGAPLDLPAAATRLDDDLILHELDAGLPSELLTGRPDIVAAEHRLKAAHASIGAARAAFFPRIALTASAGSA
SAALDGLFEPGSGAWRFAPTISLPIFDAGRNRAGLDLAEVRRDLAVASYEKTVQQAFREVADALAARHWLEAQLGIQRAA
LATQVERARLAKLRYDNGAARYLEVLDAQRDLLAAEQQVVQTRRALLSSRVALYAALGGGAQEPERTPR
>Mature_469_residues
MRPVVFGLALTGVAACSLAPPYELPALPVESRYPADAPVAPAAQRAADTSWQDYFADPALRRLIGQALDNSRDLRAAVLR
VEEARAAFGIRRADRFPTVGLGFEGVRGRTPADLSITGEAVTSGQYQMGISLASWELDLWGRVRSLEGAALEEFLATEAA
RRAVTVSVVAQVADAYLTLRELDERIVLAQRTIDTRQESFRIFSRRVEVGATSRLDLAQVETLLTQAQALGAQLEQARAL
QAHALAVLVGAPLDLPAAATRLDDDLILHELDAGLPSELLTGRPDIVAAEHRLKAAHASIGAARAAFFPRIALTASAGSA
SAALDGLFEPGSGAWRFAPTISLPIFDAGRNRAGLDLAEVRRDLAVASYEKTVQQAFREVADALAARHWLEAQLGIQRAA
LATQVERARLAKLRYDNGAARYLEVLDAQRDLLAAEQQVVQTRRALLSSRVALYAALGGGAQEPERTPR

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=459, Percent_Identity=40.0871459694989, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI2367348, Length=490, Percent_Identity=26.9387755102041, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 50412; Mature: 50412

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPVVFGLALTGVAACSLAPPYELPALPVESRYPADAPVAPAAQRAADTSWQDYFADPAL
CCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHCCHHH
RRLIGQALDNSRDLRAAVLRVEEARAAFGIRRADRFPTVGLGFEGVRGRTPADLSITGEA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCH
VTSGQYQMGISLASWELDLWGRVRSLEGAALEEFLATEAARRAVTVSVVAQVADAYLTLR
HCCCCEEECEEEEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELDERIVLAQRTIDTRQESFRIFSRRVEVGATSRLDLAQVETLLTQAQALGAQLEQARAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QAHALAVLVGAPLDLPAAATRLDDDLILHELDAGLPSELLTGRPDIVAAEHRLKAAHASI
HHHHHHHEECCCCCCCHHHHHCCHHHHHHHHCCCCCHHHHCCCCCEEEHHHHHHHHHHHH
GAARAAFFPRIALTASAGSASAALDGLFEPGSGAWRFAPTISLPIFDAGRNRAGLDLAEV
HHHHHHHHCHHEEEECCCCHHHHHHCCCCCCCCCEEECCEEECEEEECCCCCCCCCHHHH
RRDLAVASYEKTVQQAFREVADALAARHWLEAQLGIQRAALATQVERARLAKLRYDNGAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
RYLEVLDAQRDLLAAEQQVVQTRRALLSSRVALYAALGGGAQEPERTPR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MRPVVFGLALTGVAACSLAPPYELPALPVESRYPADAPVAPAAQRAADTSWQDYFADPAL
CCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHCCHHH
RRLIGQALDNSRDLRAAVLRVEEARAAFGIRRADRFPTVGLGFEGVRGRTPADLSITGEA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCH
VTSGQYQMGISLASWELDLWGRVRSLEGAALEEFLATEAARRAVTVSVVAQVADAYLTLR
HCCCCEEECEEEEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELDERIVLAQRTIDTRQESFRIFSRRVEVGATSRLDLAQVETLLTQAQALGAQLEQARAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QAHALAVLVGAPLDLPAAATRLDDDLILHELDAGLPSELLTGRPDIVAAEHRLKAAHASI
HHHHHHHEECCCCCCCHHHHHCCHHHHHHHHCCCCCHHHHCCCCCEEEHHHHHHHHHHHH
GAARAAFFPRIALTASAGSASAALDGLFEPGSGAWRFAPTISLPIFDAGRNRAGLDLAEV
HHHHHHHHCHHEEEECCCCHHHHHHCCCCCCCCCEEECCEEECEEEECCCCCCCCCHHHH
RRDLAVASYEKTVQQAFREVADALAARHWLEAQLGIQRAALATQVERARLAKLRYDNGAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
RYLEVLDAQRDLLAAEQQVVQTRRALLSSRVALYAALGGGAQEPERTPR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]