Click here to switch to the map view.

The map label for this gene is purH [H]

Identifier: 119899184

GI number: 119899184

Start: 3176279

End: 3177862

Strand: Direct

Name: purH [H]

Synonym: azo2894

Alternate gene names: 119899184

Gene position: 3176279-3177862 (Clockwise)

Preceding gene: 119899183

Following gene: 119899185

Centisome position: 72.58

GC content: 68.37

Gene sequence:

>1584_bases
ATGAACGTCACCCAAGCCCTGATCAGTGTTTCCGACAAACGCGGCGTGCTCGACTTCGCCCGCGAACTCGCCGGCCTCGG
CATCAAGCTGCTGTCGACCGGCGGCACCGCCGCCTTGCTGCGCGACGCCGGCCTGCCGGTCACCGACGTGTCCGAGCACA
CCGGCTTCCCGGAAATGCTGGACGGCCGGGTGAAGACCCTGCACCCGAAGGTGCACGGCGGCATCCTTGCCCGCCGCGAC
CTCGCCGAGCACATGGACACCATCGCCGCCCACGACATCAGCCGCATCGATCTCGTGGTGGTGAACCTCTACCCCTTCCA
GGCCACCGTGGCCCGCCCCGACTGCACGCTGGAAGACGCGATCGAGAACATCGACATCGGCGGCCCCACCATGGTGCGCG
CCGCGGCCAAGAACCACGGCACCGAAGCCGGCGGCGTCGGCATCGTCACCGATCCGGAGGACTACGCCGGCATCGTCGCC
GAGCTCAAGGCCAACGCCAACACGCTCAGCTACAAGACCCGCTTCGCGCTGGCGGTGAAGGCCTTCACCCACACCGCGCG
CTACGACTCGGCGATCTCCAACCACCTCACCGCGCTGGTCACCAACGACGCCGGCGACGTCTCCAAGCAGGCCTATCCGG
AGCGCTTCCAGCTCGCCTTCGACAAGGTGCAGAACCTGCGCTACGGCGAGAACCCGCACCAGAGCGCCGCGTTCTACAAG
GAGCCGGGCGCGGCCGAAGGCGGCATCGCCAGCTACACCCAGCTGCAGGGCAAGGAACTGTCGTACAACAACATCGCCGA
CGCCGACGCGGCGTGGGAATGCGTGAAGGCCTTCGACACCGGCGCGTGCGTCATCGTCAAGCACGCCAACCCCTGTGGCG
TCGCACTCGGCGCTACCCCGCTGGAAGCCTACAAGAAGGCCTTCTCCACCGACCCGACTTCCGCCTTCGGCGGCATCATC
GCCTTCAATGGCGAAGTCGACCGCGCCGCGGCCGAAGCGGTGTCCGCCCAGTTCCTCGAAGTGCTGATCGCGCCCTCCTA
CACCGCCGACGCACTGGAGCTGCTGCGCGCCAAGCAGAACGTGCGCGTGCTCACCTGCCCGCTCGGCAAGCCTTCCGGTG
CGCTCGACTACAAGCGCGTCGGCGGCGGCCTGCTGGTGCAGAGCGCCGACGAGGCCCGCATCCAGCTCGCCGACCTCAAG
GTCGTCACCAAGCGCGCGCCGTCCGACACCGAACTGCGCGACATGCTGTTCGCCTGGCGCGTGGCCAAGTACGTCAAGTC
CAACGCCATCGTCTATTGCAAGGACGGCATGACCATCGGCGTCGGCGCCGGCCAGATGAGCCGCGTGGACTCCGCCCGCA
TCGCCAAGATCAAGGCCGAGAACGCCGGCCTCGCCATCCCCGGCTGCGTGGTCGCGTCGGATGCCTTCTTCCCGTTCCGC
GACGGCCTCGACGTGCTCGCCCAGGCCGGCGCCACCGCGGTGATCCAGCCGGGCGGCTCGATGCGCGACGCTGAGGTGAT
CGCCGCCGCCGACGAGCAGAACATCGCGATGGTCTTCACCGGCTTCCGCCACTTCCGCCACTGA

Upstream 100 bases:

>100_bases
GGCCGCGGCGCAGCGCGCCGCCTGTTTGCCCCGCGCCGGCCACCGGGCCGCGCTGCAGTTTCCGCCTTTCCCCGCTTTCC
ACCTTGTGCAGCCTCTGAAG

Downstream 100 bases:

>100_bases
TCGGAGTCCGCACATGAAAGTCCTCGTCATCGGCTCGGGCGGACGCGAACACGCGCTGGCCTGGAAGCTCGCGCAGTCGC
CGCGCGTGACCCGCGTGCTG

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MNVTQALISVSDKRGVLDFARELAGLGIKLLSTGGTAALLRDAGLPVTDVSEHTGFPEMLDGRVKTLHPKVHGGILARRD
LAEHMDTIAAHDISRIDLVVVNLYPFQATVARPDCTLEDAIENIDIGGPTMVRAAAKNHGTEAGGVGIVTDPEDYAGIVA
ELKANANTLSYKTRFALAVKAFTHTARYDSAISNHLTALVTNDAGDVSKQAYPERFQLAFDKVQNLRYGENPHQSAAFYK
EPGAAEGGIASYTQLQGKELSYNNIADADAAWECVKAFDTGACVIVKHANPCGVALGATPLEAYKKAFSTDPTSAFGGII
AFNGEVDRAAAEAVSAQFLEVLIAPSYTADALELLRAKQNVRVLTCPLGKPSGALDYKRVGGGLLVQSADEARIQLADLK
VVTKRAPSDTELRDMLFAWRVAKYVKSNAIVYCKDGMTIGVGAGQMSRVDSARIAKIKAENAGLAIPGCVVASDAFFPFR
DGLDVLAQAGATAVIQPGGSMRDAEVIAAADEQNIAMVFTGFRHFRH

Sequences:

>Translated_527_residues
MNVTQALISVSDKRGVLDFARELAGLGIKLLSTGGTAALLRDAGLPVTDVSEHTGFPEMLDGRVKTLHPKVHGGILARRD
LAEHMDTIAAHDISRIDLVVVNLYPFQATVARPDCTLEDAIENIDIGGPTMVRAAAKNHGTEAGGVGIVTDPEDYAGIVA
ELKANANTLSYKTRFALAVKAFTHTARYDSAISNHLTALVTNDAGDVSKQAYPERFQLAFDKVQNLRYGENPHQSAAFYK
EPGAAEGGIASYTQLQGKELSYNNIADADAAWECVKAFDTGACVIVKHANPCGVALGATPLEAYKKAFSTDPTSAFGGII
AFNGEVDRAAAEAVSAQFLEVLIAPSYTADALELLRAKQNVRVLTCPLGKPSGALDYKRVGGGLLVQSADEARIQLADLK
VVTKRAPSDTELRDMLFAWRVAKYVKSNAIVYCKDGMTIGVGAGQMSRVDSARIAKIKAENAGLAIPGCVVASDAFFPFR
DGLDVLAQAGATAVIQPGGSMRDAEVIAAADEQNIAMVFTGFRHFRH
>Mature_527_residues
MNVTQALISVSDKRGVLDFARELAGLGIKLLSTGGTAALLRDAGLPVTDVSEHTGFPEMLDGRVKTLHPKVHGGILARRD
LAEHMDTIAAHDISRIDLVVVNLYPFQATVARPDCTLEDAIENIDIGGPTMVRAAAKNHGTEAGGVGIVTDPEDYAGIVA
ELKANANTLSYKTRFALAVKAFTHTARYDSAISNHLTALVTNDAGDVSKQAYPERFQLAFDKVQNLRYGENPHQSAAFYK
EPGAAEGGIASYTQLQGKELSYNNIADADAAWECVKAFDTGACVIVKHANPCGVALGATPLEAYKKAFSTDPTSAFGGII
AFNGEVDRAAAEAVSAQFLEVLIAPSYTADALELLRAKQNVRVLTCPLGKPSGALDYKRVGGGLLVQSADEARIQLADLK
VVTKRAPSDTELRDMLFAWRVAKYVKSNAIVYCKDGMTIGVGAGQMSRVDSARIAKIKAENAGLAIPGCVVASDAFFPFR
DGLDVLAQAGATAVIQPGGSMRDAEVIAAADEQNIAMVFTGFRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=485, Percent_Identity=38.9690721649485, Blast_Score=277, Evalue=2e-74,
Organism=Escherichia coli, GI1790439, Length=530, Percent_Identity=61.1320754716981, Blast_Score=629, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=529, Percent_Identity=33.8374291115312, Blast_Score=245, Evalue=3e-65,
Organism=Caenorhabditis elegans, GI71985574, Length=355, Percent_Identity=25.6338028169014, Blast_Score=82, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323056, Length=491, Percent_Identity=37.6782077393075, Blast_Score=271, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6323768, Length=487, Percent_Identity=36.9609856262834, Blast_Score=270, Evalue=5e-73,
Organism=Drosophila melanogaster, GI24649832, Length=495, Percent_Identity=40.8080808080808, Blast_Score=291, Evalue=7e-79,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 55950; Mature: 55950

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVTQALISVSDKRGVLDFARELAGLGIKLLSTGGTAALLRDAGLPVTDVSEHTGFPEML
CCHHHHHHHHCCCCCHHHHHHHHHCCCEEEEECCCCEEEHHHCCCCCCCCHHHCCCHHHH
DGRVKTLHPKVHGGILARRDLAEHMDTIAAHDISRIDLVVVNLYPFQATVARPDCTLEDA
CCCCEEECCHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCEEEECCCCCCHHHH
IENIDIGGPTMVRAAAKNHGTEAGGVGIVTDPEDYAGIVAELKANANTLSYKTRFALAVK
HHHCCCCCCHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEHHHHEEEHH
AFTHTARYDSAISNHLTALVTNDAGDVSKQAYPERFQLAFDKVQNLRYGENPHQSAAFYK
HHHHHHHHHHHHHCCEEEEEECCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCEEC
EPGAAEGGIASYTQLQGKELSYNNIADADAAWECVKAFDTGACVIVKHANPCGVALGATP
CCCCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCEEECCCH
LEAYKKAFSTDPTSAFGGIIAFNGEVDRAAAEAVSAQFLEVLIAPSYTADALELLRAKQN
HHHHHHHHCCCCCHHHCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCC
VRVLTCPLGKPSGALDYKRVGGGLLVQSADEARIQLADLKVVTKRAPSDTELRDMLFAWR
CEEEEECCCCCCCCCCHHHCCCEEEEECCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHH
VAKYVKSNAIVYCKDGMTIGVGAGQMSRVDSARIAKIKAENAGLAIPGCVVASDAFFPFR
HHHHHHCCCEEEEECCCEEEECCCCHHHCCCCCEEEEEECCCCEECCCCEEECCCCCCCC
DGLDVLAQAGATAVIQPGGSMRDAEVIAAADEQNIAMVFTGFRHFRH
CCHHHHHHCCCEEEECCCCCCCCCEEEEECCCCCEEEEEECHHHHCC
>Mature Secondary Structure
MNVTQALISVSDKRGVLDFARELAGLGIKLLSTGGTAALLRDAGLPVTDVSEHTGFPEML
CCHHHHHHHHCCCCCHHHHHHHHHCCCEEEEECCCCEEEHHHCCCCCCCCHHHCCCHHHH
DGRVKTLHPKVHGGILARRDLAEHMDTIAAHDISRIDLVVVNLYPFQATVARPDCTLEDA
CCCCEEECCHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCEEEECCCCCCHHHH
IENIDIGGPTMVRAAAKNHGTEAGGVGIVTDPEDYAGIVAELKANANTLSYKTRFALAVK
HHHCCCCCCHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEHHHHEEEHH
AFTHTARYDSAISNHLTALVTNDAGDVSKQAYPERFQLAFDKVQNLRYGENPHQSAAFYK
HHHHHHHHHHHHHCCEEEEEECCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCEEC
EPGAAEGGIASYTQLQGKELSYNNIADADAAWECVKAFDTGACVIVKHANPCGVALGATP
CCCCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCEEECCCH
LEAYKKAFSTDPTSAFGGIIAFNGEVDRAAAEAVSAQFLEVLIAPSYTADALELLRAKQN
HHHHHHHHCCCCCHHHCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCC
VRVLTCPLGKPSGALDYKRVGGGLLVQSADEARIQLADLKVVTKRAPSDTELRDMLFAWR
CEEEEECCCCCCCCCCHHHCCCEEEEECCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHH
VAKYVKSNAIVYCKDGMTIGVGAGQMSRVDSARIAKIKAENAGLAIPGCVVASDAFFPFR
HHHHHHCCCEEEEECCCEEEECCCCHHHCCCCCEEEEEECCCCEECCCCEEECCCCCCCC
DGLDVLAQAGATAVIQPGGSMRDAEVIAAADEQNIAMVFTGFRHFRH
CCHHHHHHCCCEEEECCCCCCCCCEEEEECCCCCEEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA