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The map label for this gene is dusB [H]

Identifier: 119899182

GI number: 119899182

Start: 3174872

End: 3175945

Strand: Direct

Name: dusB [H]

Synonym: azo2892

Alternate gene names: 119899182

Gene position: 3174872-3175945 (Clockwise)

Preceding gene: 119899181

Following gene: 119899183

Centisome position: 72.55

GC content: 65.55

Gene sequence:

>1074_bases
ATGCCGGCCTTTTCCCGCCCGCGCCCTCCCATGCAGTTTGCCGGATTCTCCTTGCGCAACAATCTCTTCGTCGCACCGAT
GGCCGGTGTGACAGATCGCCCTTTCCGCCAGTTGTGCAAGAAATTGGGCGCCGGCCTCGCGGTGTCGGAGATGGTCACCT
CCAACTCGCTGCTGTACGGCAGCGCCAAGACGCAGCGGCGCGCCAACCACGACGGCGAGGTCGATCCGATCTCGGTGCAG
ATCGCCGGCGCCGACCCGGCGATGATGGCGGAGGCCGCGCGCCACAACGCCGACCGCGGCGCGCAGATCATCGACATCAA
CATGGGCTGCCCGGCCAAGAAGGTGTGCAACGTGATGGCCGGCTCGGCGCTGATGCAGGACGAGCCGCTGGTGGCGCGCA
TCCTCGAAGCCGTGGTGAAGGCGGTGCCCGATACCCCGGTCACGCTCAAGTTCCGCACCGGCTGGAACCGCCAGAACAAG
AACGCCCCGATCATCGCCCGCATTGCCGAAGAGTCCGGCATCCAGGGCATCGCCATCCACGGCCGCACCCGCGCCGACCA
GTACATGGGCGATGCCGAATACGACACCATCGCGCTGGTGAAGACGCTGGTGAAGATCCCGGTGATCGCAAACGGCGACA
TCACCACGCCGCAGAAGGCCAGACATGTGCTCGACTACACCGGCGCTGACGGCGTGATGATCGGCCGCGCCGCCCAGGGC
CGGCCGTGGATCTTCCGCGAGATCGAACATTTCCTCGCGACCGGCGAGGAACTGCCCCCGCCGCTGGTTTCCGACATCCA
CCAGGTGTGCCGTGAGCACCTCGCCGACCTCTACGCCTTTTACGGCGAAGACACCGGCGTGAAGGTCGCGCGCAAGCACA
TCTCCTGGTACACCAAGGGTCTGGTCGGCTCCGCCGCCTTCCGGCGCACGATGAACCAGCTGCCCGACATCCCCGCCCAG
CTCGCTGCGGTAGACGAATTCTTCGGTCAGCTTGCCGAGGCGGACAGCCGTTTGAGTTATCAGACCGGCCAAGCCGATTT
TGGCGACGAACAGCCTCAGGAACTTGCAGCATGA

Upstream 100 bases:

>100_bases
GCGGATGATGTTCGGCGCGCGCGCCTGGCCGTAACCGGGATCACGGCGCGGCGCCCGTCGCGGGCGCTGCGCTCGCCCAC
CCCCGCCTGCATGGGGTAAA

Downstream 100 bases:

>100_bases
GCCGCAGCAACGAGATCGCCGACTCCGTCTTCCGGACCCTCGACCAGTATTTCCGCGATCTCGACGGCGAGAAGCCGTCG
GCGATCTACGACATGGTGAT

Product: putative tRNA-dihydrouridine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MPAFSRPRPPMQFAGFSLRNNLFVAPMAGVTDRPFRQLCKKLGAGLAVSEMVTSNSLLYGSAKTQRRANHDGEVDPISVQ
IAGADPAMMAEAARHNADRGAQIIDINMGCPAKKVCNVMAGSALMQDEPLVARILEAVVKAVPDTPVTLKFRTGWNRQNK
NAPIIARIAEESGIQGIAIHGRTRADQYMGDAEYDTIALVKTLVKIPVIANGDITTPQKARHVLDYTGADGVMIGRAAQG
RPWIFREIEHFLATGEELPPPLVSDIHQVCREHLADLYAFYGEDTGVKVARKHISWYTKGLVGSAAFRRTMNQLPDIPAQ
LAAVDEFFGQLAEADSRLSYQTGQADFGDEQPQELAA

Sequences:

>Translated_357_residues
MPAFSRPRPPMQFAGFSLRNNLFVAPMAGVTDRPFRQLCKKLGAGLAVSEMVTSNSLLYGSAKTQRRANHDGEVDPISVQ
IAGADPAMMAEAARHNADRGAQIIDINMGCPAKKVCNVMAGSALMQDEPLVARILEAVVKAVPDTPVTLKFRTGWNRQNK
NAPIIARIAEESGIQGIAIHGRTRADQYMGDAEYDTIALVKTLVKIPVIANGDITTPQKARHVLDYTGADGVMIGRAAQG
RPWIFREIEHFLATGEELPPPLVSDIHQVCREHLADLYAFYGEDTGVKVARKHISWYTKGLVGSAAFRRTMNQLPDIPAQ
LAAVDEFFGQLAEADSRLSYQTGQADFGDEQPQELAA
>Mature_356_residues
PAFSRPRPPMQFAGFSLRNNLFVAPMAGVTDRPFRQLCKKLGAGLAVSEMVTSNSLLYGSAKTQRRANHDGEVDPISVQI
AGADPAMMAEAARHNADRGAQIIDINMGCPAKKVCNVMAGSALMQDEPLVARILEAVVKAVPDTPVTLKFRTGWNRQNKN
APIIARIAEESGIQGIAIHGRTRADQYMGDAEYDTIALVKTLVKIPVIANGDITTPQKARHVLDYTGADGVMIGRAAQGR
PWIFREIEHFLATGEELPPPLVSDIHQVCREHLADLYAFYGEDTGVKVARKHISWYTKGLVGSAAFRRTMNQLPDIPAQL
AAVDEFFGQLAEADSRLSYQTGQADFGDEQPQELAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=287, Percent_Identity=29.616724738676, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI239788462, Length=298, Percent_Identity=29.1946308724832, Blast_Score=118, Evalue=6e-27,
Organism=Homo sapiens, GI31742496, Length=245, Percent_Identity=31.0204081632653, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI40807366, Length=300, Percent_Identity=28, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI8923374, Length=254, Percent_Identity=27.9527559055118, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1789660, Length=322, Percent_Identity=55.5900621118012, Blast_Score=355, Evalue=3e-99,
Organism=Escherichia coli, GI1788462, Length=318, Percent_Identity=28.3018867924528, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI145693211, Length=239, Percent_Identity=29.7071129707113, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17543114, Length=256, Percent_Identity=29.6875, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI25144369, Length=308, Percent_Identity=25.3246753246753, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17510279, Length=245, Percent_Identity=27.3469387755102, Blast_Score=68, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6323433, Length=298, Percent_Identity=26.8456375838926, Blast_Score=102, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6323560, Length=236, Percent_Identity=30.0847457627119, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323437, Length=224, Percent_Identity=26.3392857142857, Blast_Score=76, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24585320, Length=288, Percent_Identity=28.8194444444444, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24580595, Length=252, Percent_Identity=30.5555555555556, Blast_Score=95, Evalue=8e-20,
Organism=Drosophila melanogaster, GI19920448, Length=252, Percent_Identity=30.5555555555556, Blast_Score=95, Evalue=8e-20,
Organism=Drosophila melanogaster, GI19921524, Length=224, Percent_Identity=27.2321428571429, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI45549423, Length=258, Percent_Identity=31.0077519379845, Blast_Score=81, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 38864; Mature: 38733

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAFSRPRPPMQFAGFSLRNNLFVAPMAGVTDRPFRQLCKKLGAGLAVSEMVTSNSLLYG
CCCCCCCCCCHHHCCEECCCCEEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHCCCEEEC
SAKTQRRANHDGEVDPISVQIAGADPAMMAEAARHNADRGAQIIDINMGCPAKKVCNVMA
CCHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHH
GSALMQDEPLVARILEAVVKAVPDTPVTLKFRTGWNRQNKNAPIIARIAEESGIQGIAIH
CCHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEEHHHCCCCEEEEE
GRTRADQYMGDAEYDTIALVKTLVKIPVIANGDITTPQKARHVLDYTGADGVMIGRAAQG
CCCHHHHHCCCCCHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHCCCCCCEEEECCCCC
RPWIFREIEHFLATGEELPPPLVSDIHQVCREHLADLYAFYGEDTGVKVARKHISWYTKG
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LVGSAAFRRTMNQLPDIPAQLAAVDEFFGQLAEADSRLSYQTGQADFGDEQPQELAA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCC
>Mature Secondary Structure 
PAFSRPRPPMQFAGFSLRNNLFVAPMAGVTDRPFRQLCKKLGAGLAVSEMVTSNSLLYG
CCCCCCCCCHHHCCEECCCCEEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHCCCEEEC
SAKTQRRANHDGEVDPISVQIAGADPAMMAEAARHNADRGAQIIDINMGCPAKKVCNVMA
CCHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHH
GSALMQDEPLVARILEAVVKAVPDTPVTLKFRTGWNRQNKNAPIIARIAEESGIQGIAIH
CCHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEEHHHCCCCEEEEE
GRTRADQYMGDAEYDTIALVKTLVKIPVIANGDITTPQKARHVLDYTGADGVMIGRAAQG
CCCHHHHHCCCCCHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHCCCCCCEEEECCCCC
RPWIFREIEHFLATGEELPPPLVSDIHQVCREHLADLYAFYGEDTGVKVARKHISWYTKG
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LVGSAAFRRTMNQLPDIPAQLAAVDEFFGQLAEADSRLSYQTGQADFGDEQPQELAA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]