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The map label for this gene is ostA [H]

Identifier: 119899177

GI number: 119899177

Start: 3167868

End: 3169991

Strand: Reverse

Name: ostA [H]

Synonym: azo2887

Alternate gene names: 119899177

Gene position: 3169991-3167868 (Counterclockwise)

Preceding gene: 119899192

Following gene: 119899176

Centisome position: 72.44

GC content: 67.04

Gene sequence:

>2124_bases
GTGGAACTGGTCGCAGAGGGCGATGCCGAGCTGCAGCGCGACGACATCCTGCTGACCGCCGACGCGCTGACCTACCGCGA
ACCCACGGACGAGGCGCTGGCCGAGGGCAATGTGCGCCTGCGCCAGGGGCCGGACGTGATCTCCGGCCCGTCCGCCAGCC
TCGTCGTGGGTGAGCGCACCGGCGACTTCCAGTCGCCGCGCTACGAGATCACCCGCACCAAGGCGGGGCTGGAACCCGGA
GACCCGCCGCGCCAGGTATCTGGCGGCGGTCACGCGGACGTGCTCCATTTCGAAGGCGAGAACCAGTACCGGCTGGAGAA
CGCCACCTGGAGCACCTGTTCGGTGGACGATCCCGACTGGTACATCAAGGCGCGCGACCTGCAGCTCGATTACGACCGCG
AGATCGGCGTCGCCAAGGGCGGCACCGTGGTGTTCAAGGATGTGCCGTTCTTCTGGATGCCGTGGGCCGAGTTCCCGCTG
GTGGGCCAGCGCCAGTCGGGCGTGCTGCCGCCCACGATCGGTTCGTCCAACAAGACCGGTCTTGACCTCACCGTCCCGTA
TTACTGGAACATCGCGCCGAACTACGACGCCACCATCACCCCGCGCTACATGGGGCGCCGTGGCCTGCAGTTGGGCGGTG
AATTCCGCTATCTGGGCGACTCCTACGCCGGCGAAGTGCGGGCCGAATGGCTGGGCCGCGACCGCATCACCGGCGAGCAG
CGCACGCTGGGCTCCTTGCAGCACCGCCAGCAGATCACCTCTTCGCTGTATGGCTCGCTCGACATCAACGGTGTGTCCGA
CGACGACTACTTCGAGGATCTGAGTTCGCGCGTGTCGGTTGCCTCCCGCGTCAATCTGCTGCGCGAAGGGCGTCTGGTGT
ATGTCGGCAGCCCGTGGTGGAGCGCCTCGGCGCTGGTCCAGAGCTACCAGACGCTGAACTCCGATCCGGACAATCCGGTG
ACCACGCCCTACCGTCGCGTGCCGCAACTGCTGACCACCGCCAGCCGCCCCGATCTGCCCGCCGGTCTCACGTTCAACTG
GCACGGCGAATACGTGCAGTTCGCCCACCCGGACGAGGATCATCCGGAGGGCAACCGCTTCACCGCCTATCCGCAGCTGT
CGCTGCCGATGCAGCGCGCCGGGTTTTACGTGACGCCCAAGGTCGGCGTCCACTACACCCGCTACGACCTCGACGAACCG
GTCGCGGGCCTCACCGATGCGCGCACCTCGATCACGCGCACCGTGCCGATCTTCTCGGTCGACTCGGGCGTCACCTTCGA
GCGCGACGCGCAGTTCTTCGGCACCGCCTTCACCCAGACGCTGGAACCGCGGCTGTACTACCTGAACGTGCCCTACCGCC
GCCAGAACGACATTCCGCTGTTCGACACCAGCCGCTACGACTTCGGCTTCGCGCAGATCTTTGCCGAGAACCGCTACACC
GGCGGCGACCGCATCGGCGACGCGAACCAGCTCACCGCTGCGGTCACCACCCGGCTCATCGACCCCGAAACCGGTTCCGA
GCGCATGCGCGCGCTTGTCGGCCAGCGCTACTACTTCGAAGACCAGCGCGTCACCGCCGGCGAAGCCTTGCGCACCAGCC
GCCGCACCGACGTGCTCGGCGGTTTCAGCGGCCGCATCACGCGCTACAGCACCGTCGATACGCTGCTGCAGTACAACCCG
GAAGACAACGAGACCGAGCGCTTCAACTTCACCTTCCGCTACCAGCCCGAGTTCGCCAAGGCGCTCAACCTGGGTTACCG
CTACGCCCGCAGCCTGGTCAGCCCGGACGGCACGATCGGTCTGCGCGACGTGAACGTGTCCGGGCAGTGGCCGCTCGGCG
GCGGCTGGTACGGCGTTGGCCGGCTGACGCATTCGCTGAAGGACGACCGCCTGACCGAGGCGATTGCCGGCATCGAGTAC
GACGGCGGCTGCTGGGTTGCGCGCGTGGCGATGCACCGCTTCGCGACCGACCCCAACGATGTCACCAAGGCGGTGTTCAT
CCAGCTCGAACTCAACGACCTCGCCAGCATCGGCTCCAGCCCGGTCAACCTGATCAAGCGCAGCGTGCCGGGCTATGGCA
AGATCAACGACCCCGCCGCCAACCGCGTGTTCGGCATCGAATGA

Upstream 100 bases:

>100_bases
TTCCGCCGCCCCGGCTGCGTCCGCAAGCCCGGCCGACGCGACGGCGCGCAAGCCCGGCTCCACCGAGGTTCGCGCACTGC
GCATCCGTGGCACCCGGTCG

Downstream 100 bases:

>100_bases
CGCAGCACGCGCCTCCATCTGCCTGACCAAGCTCATGAATCGATTCCCGCTTCGCCCGCGTTCCCTTTTCGTCGCCCTGG
TTGCCTGCAGCGCGCTCGCG

Product: organic solvent tolerance protein

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 707; Mature: 707

Protein sequence:

>707_residues
MELVAEGDAELQRDDILLTADALTYREPTDEALAEGNVRLRQGPDVISGPSASLVVGERTGDFQSPRYEITRTKAGLEPG
DPPRQVSGGGHADVLHFEGENQYRLENATWSTCSVDDPDWYIKARDLQLDYDREIGVAKGGTVVFKDVPFFWMPWAEFPL
VGQRQSGVLPPTIGSSNKTGLDLTVPYYWNIAPNYDATITPRYMGRRGLQLGGEFRYLGDSYAGEVRAEWLGRDRITGEQ
RTLGSLQHRQQITSSLYGSLDINGVSDDDYFEDLSSRVSVASRVNLLREGRLVYVGSPWWSASALVQSYQTLNSDPDNPV
TTPYRRVPQLLTTASRPDLPAGLTFNWHGEYVQFAHPDEDHPEGNRFTAYPQLSLPMQRAGFYVTPKVGVHYTRYDLDEP
VAGLTDARTSITRTVPIFSVDSGVTFERDAQFFGTAFTQTLEPRLYYLNVPYRRQNDIPLFDTSRYDFGFAQIFAENRYT
GGDRIGDANQLTAAVTTRLIDPETGSERMRALVGQRYYFEDQRVTAGEALRTSRRTDVLGGFSGRITRYSTVDTLLQYNP
EDNETERFNFTFRYQPEFAKALNLGYRYARSLVSPDGTIGLRDVNVSGQWPLGGGWYGVGRLTHSLKDDRLTEAIAGIEY
DGGCWVARVAMHRFATDPNDVTKAVFIQLELNDLASIGSSPVNLIKRSVPGYGKINDPAANRVFGIE

Sequences:

>Translated_707_residues
MELVAEGDAELQRDDILLTADALTYREPTDEALAEGNVRLRQGPDVISGPSASLVVGERTGDFQSPRYEITRTKAGLEPG
DPPRQVSGGGHADVLHFEGENQYRLENATWSTCSVDDPDWYIKARDLQLDYDREIGVAKGGTVVFKDVPFFWMPWAEFPL
VGQRQSGVLPPTIGSSNKTGLDLTVPYYWNIAPNYDATITPRYMGRRGLQLGGEFRYLGDSYAGEVRAEWLGRDRITGEQ
RTLGSLQHRQQITSSLYGSLDINGVSDDDYFEDLSSRVSVASRVNLLREGRLVYVGSPWWSASALVQSYQTLNSDPDNPV
TTPYRRVPQLLTTASRPDLPAGLTFNWHGEYVQFAHPDEDHPEGNRFTAYPQLSLPMQRAGFYVTPKVGVHYTRYDLDEP
VAGLTDARTSITRTVPIFSVDSGVTFERDAQFFGTAFTQTLEPRLYYLNVPYRRQNDIPLFDTSRYDFGFAQIFAENRYT
GGDRIGDANQLTAAVTTRLIDPETGSERMRALVGQRYYFEDQRVTAGEALRTSRRTDVLGGFSGRITRYSTVDTLLQYNP
EDNETERFNFTFRYQPEFAKALNLGYRYARSLVSPDGTIGLRDVNVSGQWPLGGGWYGVGRLTHSLKDDRLTEAIAGIEY
DGGCWVARVAMHRFATDPNDVTKAVFIQLELNDLASIGSSPVNLIKRSVPGYGKINDPAANRVFGIE
>Mature_707_residues
MELVAEGDAELQRDDILLTADALTYREPTDEALAEGNVRLRQGPDVISGPSASLVVGERTGDFQSPRYEITRTKAGLEPG
DPPRQVSGGGHADVLHFEGENQYRLENATWSTCSVDDPDWYIKARDLQLDYDREIGVAKGGTVVFKDVPFFWMPWAEFPL
VGQRQSGVLPPTIGSSNKTGLDLTVPYYWNIAPNYDATITPRYMGRRGLQLGGEFRYLGDSYAGEVRAEWLGRDRITGEQ
RTLGSLQHRQQITSSLYGSLDINGVSDDDYFEDLSSRVSVASRVNLLREGRLVYVGSPWWSASALVQSYQTLNSDPDNPV
TTPYRRVPQLLTTASRPDLPAGLTFNWHGEYVQFAHPDEDHPEGNRFTAYPQLSLPMQRAGFYVTPKVGVHYTRYDLDEP
VAGLTDARTSITRTVPIFSVDSGVTFERDAQFFGTAFTQTLEPRLYYLNVPYRRQNDIPLFDTSRYDFGFAQIFAENRYT
GGDRIGDANQLTAAVTTRLIDPETGSERMRALVGQRYYFEDQRVTAGEALRTSRRTDVLGGFSGRITRYSTVDTLLQYNP
EDNETERFNFTFRYQPEFAKALNLGYRYARSLVSPDGTIGLRDVNVSGQWPLGGGWYGVGRLTHSLKDDRLTEAIAGIEY
DGGCWVARVAMHRFATDPNDVTKAVFIQLELNDLASIGSSPVNLIKRSVPGYGKINDPAANRVFGIE

Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

Organism=Escherichia coli, GI1786239, Length=717, Percent_Identity=27.7545327754533, Blast_Score=239, Evalue=4e-64,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543 [H]

Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 79114; Mature: 79114

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELVAEGDAELQRDDILLTADALTYREPTDEALAEGNVRLRQGPDVISGPSASLVVGERT
CCEECCCCCCCCCCCEEEEECCEECCCCCHHHHHCCCEEEECCCCCCCCCCCEEEEECCC
GDFQSPRYEITRTKAGLEPGDPPRQVSGGGHADVLHFEGENQYRLENATWSTCSVDDPDW
CCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCCCCE
YIKARDLQLDYDREIGVAKGGTVVFKDVPFFWMPWAEFPLVGQRQSGVLPPTIGSSNKTG
EEEEEEEEECCCCCCCEECCCEEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
LDLTVPYYWNIAPNYDATITPRYMGRRGLQLGGEFRYLGDSYAGEVRAEWLGRDRITGEQ
EEEECCEEEECCCCCCCEECHHHHCCCCCCCCCEEEECCCCCCCCHHHHHCCCCCCCCCH
RTLGSLQHRQQITSSLYGSLDINGVSDDDYFEDLSSRVSVASRVNLLREGRLVYVGSPWW
HHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
SASALVQSYQTLNSDPDNPVTTPYRRVPQLLTTASRPDLPAGLTFNWHGEYVQFAHPDED
CHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCEEEECCCCCC
HPEGNRFTAYPQLSLPMQRAGFYVTPKVGVHYTRYDLDEPVAGLTDARTSITRTVPIFSV
CCCCCEEEECCCCCCCHHHCCEEEECCCCEEEEEECCCCCCCCCCCCCHHHEEEEEEEEC
DSGVTFERDAQFFGTAFTQTLEPRLYYLNVPYRRQNDIPLFDTSRYDFGFAQIFAENRYT
CCCCEECCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEECCCCCCCHHHEEECCCCC
GGDRIGDANQLTAAVTTRLIDPETGSERMRALVGQRYYFEDQRVTAGEALRTSRRTDVLG
CCCCCCCHHHHHHHHHHEECCCCCCHHHHHHHHCCCEEECCCCCCHHHHHHHCCCHHHCC
GFSGRITRYSTVDTLLQYNPEDNETERFNFTFRYQPEFAKALNLGYRYARSLVSPDGTIG
CCCCCEEEEEHHHHHHEECCCCCCCEEEEEEEEECHHHHHHHHHHHHHHHHHCCCCCCEE
LRDVNVSGQWPLGGGWYGVGRLTHSLKDDRLTEAIAGIEYDGGCWVARVAMHRFATDPND
EEEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCEECCCHHHHHHHHHHHCCCCCC
VTKAVFIQLELNDLASIGSSPVNLIKRSVPGYGKINDPAANRVFGIE
CEEEEEEEEEHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCEECCC
>Mature Secondary Structure
MELVAEGDAELQRDDILLTADALTYREPTDEALAEGNVRLRQGPDVISGPSASLVVGERT
CCEECCCCCCCCCCCEEEEECCEECCCCCHHHHHCCCEEEECCCCCCCCCCCEEEEECCC
GDFQSPRYEITRTKAGLEPGDPPRQVSGGGHADVLHFEGENQYRLENATWSTCSVDDPDW
CCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCCCCE
YIKARDLQLDYDREIGVAKGGTVVFKDVPFFWMPWAEFPLVGQRQSGVLPPTIGSSNKTG
EEEEEEEEECCCCCCCEECCCEEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
LDLTVPYYWNIAPNYDATITPRYMGRRGLQLGGEFRYLGDSYAGEVRAEWLGRDRITGEQ
EEEECCEEEECCCCCCCEECHHHHCCCCCCCCCEEEECCCCCCCCHHHHHCCCCCCCCCH
RTLGSLQHRQQITSSLYGSLDINGVSDDDYFEDLSSRVSVASRVNLLREGRLVYVGSPWW
HHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
SASALVQSYQTLNSDPDNPVTTPYRRVPQLLTTASRPDLPAGLTFNWHGEYVQFAHPDED
CHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCEEEECCCCCC
HPEGNRFTAYPQLSLPMQRAGFYVTPKVGVHYTRYDLDEPVAGLTDARTSITRTVPIFSV
CCCCCEEEECCCCCCCHHHCCEEEECCCCEEEEEECCCCCCCCCCCCCHHHEEEEEEEEC
DSGVTFERDAQFFGTAFTQTLEPRLYYLNVPYRRQNDIPLFDTSRYDFGFAQIFAENRYT
CCCCEECCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEECCCCCCCHHHEEECCCCC
GGDRIGDANQLTAAVTTRLIDPETGSERMRALVGQRYYFEDQRVTAGEALRTSRRTDVLG
CCCCCCCHHHHHHHHHHEECCCCCCHHHHHHHHCCCEEECCCCCCHHHHHHHCCCHHHCC
GFSGRITRYSTVDTLLQYNPEDNETERFNFTFRYQPEFAKALNLGYRYARSLVSPDGTIG
CCCCCEEEEEHHHHHHEECCCCCCCEEEEEEEEECHHHHHHHHHHHHHHHHHCCCCCCEE
LRDVNVSGQWPLGGGWYGVGRLTHSLKDDRLTEAIAGIEYDGGCWVARVAMHRFATDPND
EEEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCEECCCHHHHHHHHHHHCCCCCC
VTKAVFIQLELNDLASIGSSPVNLIKRSVPGYGKINDPAANRVFGIE
CEEEEEEEEEHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA