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The map label for this gene is estB [H]

Identifier: 119899149

GI number: 119899149

Start: 3139801

End: 3140484

Strand: Direct

Name: estB [H]

Synonym: azo2859

Alternate gene names: 119899149

Gene position: 3139801-3140484 (Clockwise)

Preceding gene: 119899148

Following gene: 119899154

Centisome position: 71.75

GC content: 69.59

Gene sequence:

>684_bases
ATGCCGAACAGGATCCTGCCCGCCATCGAGATCGAGACCGGTGCGCAACCCACGCACTCGGTGATCTGGCTGCACGGACT
CGGCGCGGACGGCCACGACTTCGAGCCCATCGTGGGCGAACTCGACACCGCGCGGCTCCCGCCCACCCGCTTCCTGTTCC
CGCATGCCCCCATCCGCACCGTCACCATCAATGGCGGCTACGCGATGCGGGCTTGGTACGACATCATCTCTTCCGACTTC
TCGCAGCGGCGGGAAGACCCGCGCGGCGTGCGCGAATCGGCACAGCAGCTCGAAAACCTGATCGCGCGCGAGAACGAGCG
CGGCGTCAGCGATGCGAACATCGTGCTGGCGGGTTTTTCGCAGGGCGGCGCGGTGGCGCTGCACACCGGGCTGCGCCACC
GCCGCCGGCTGGCGGGCATCCTGGCGCTCTCCACCTATCTGCCGCTGGCTGAAACGCTGGCGGCCGAAGCCGACCCGGTC
AACCGCGACCTGCCGATCTTCATGGCCCACGGCGAGGCCGACACCGTGATCCCGCACGCCTTCGCGCGCCGCTCGGCGGA
GTTGTTGCAGGCAAGCGGCTATCCGCTGGAATGGCACAACTACCCGGTGGACCACACGGTGTCGGCCGACGAGATCCGCG
ACCTCGACGCCTGGCTGCGGCGGGTGCTGGCCCCGGCGGGCTGA

Upstream 100 bases:

>100_bases
ACACGTTGCGCACCCGGAACGCAACGGCGATTGCGCCGTACAATCGCCACCAAGCACACATGCGATGCGGATTACGATCG
ACCCCGCCGAGGAGCCCGCC

Downstream 100 bases:

>100_bases
GGCCGCTACAGGATGCGGCGGAACCACACCATCGACAGCCCCGCCGCCAGCACCATCAGCGCCGCCCCGGCGGCGGCGAA
CAGCGCGCTGACCTCGGTCT

Product: carboxylesterase

Products: NA

Alternate protein names: Esterase I [H]

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MPNRILPAIEIETGAQPTHSVIWLHGLGADGHDFEPIVGELDTARLPPTRFLFPHAPIRTVTINGGYAMRAWYDIISSDF
SQRREDPRGVRESAQQLENLIARENERGVSDANIVLAGFSQGGAVALHTGLRHRRRLAGILALSTYLPLAETLAAEADPV
NRDLPIFMAHGEADTVIPHAFARRSAELLQASGYPLEWHNYPVDHTVSADEIRDLDAWLRRVLAPAG

Sequences:

>Translated_227_residues
MPNRILPAIEIETGAQPTHSVIWLHGLGADGHDFEPIVGELDTARLPPTRFLFPHAPIRTVTINGGYAMRAWYDIISSDF
SQRREDPRGVRESAQQLENLIARENERGVSDANIVLAGFSQGGAVALHTGLRHRRRLAGILALSTYLPLAETLAAEADPV
NRDLPIFMAHGEADTVIPHAFARRSAELLQASGYPLEWHNYPVDHTVSADEIRDLDAWLRRVLAPAG
>Mature_226_residues
PNRILPAIEIETGAQPTHSVIWLHGLGADGHDFEPIVGELDTARLPPTRFLFPHAPIRTVTINGGYAMRAWYDIISSDFS
QRREDPRGVRESAQQLENLIARENERGVSDANIVLAGFSQGGAVALHTGLRHRRRLAGILALSTYLPLAETLAAEADPVN
RDLPIFMAHGEADTVIPHAFARRSAELLQASGYPLEWHNYPVDHTVSADEIRDLDAWLRRVLAPAG

Specific function: Hydrolyzes carboxylic ester bonds with relatively broad substrate specificity [H]

COG id: COG0400

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI5453722, Length=224, Percent_Identity=33.4821428571429, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI9966764, Length=211, Percent_Identity=36.9668246445498, Blast_Score=134, Evalue=6e-32,
Organism=Homo sapiens, GI20270341, Length=206, Percent_Identity=29.6116504854369, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17508057, Length=206, Percent_Identity=32.0388349514563, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6323147, Length=229, Percent_Identity=31.4410480349345, Blast_Score=122, Evalue=5e-29,
Organism=Drosophila melanogaster, GI45553055, Length=208, Percent_Identity=34.1346153846154, Blast_Score=130, Evalue=9e-31,
Organism=Drosophila melanogaster, GI45553057, Length=208, Percent_Identity=34.1346153846154, Blast_Score=130, Evalue=9e-31,
Organism=Drosophila melanogaster, GI21357257, Length=208, Percent_Identity=34.1346153846154, Blast_Score=130, Evalue=9e-31,
Organism=Drosophila melanogaster, GI161078179, Length=222, Percent_Identity=30.1801801801802, Blast_Score=85, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003140 [H]

Pfam domain/function: PF02230 Abhydrolase_2 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 25028; Mature: 24896

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNRILPAIEIETGAQPTHSVIWLHGLGADGHDFEPIVGELDTARLPPTRFLFPHAPIRT
CCCCCCCEEEECCCCCCCCEEEEEEECCCCCCCCCHHHCCCCCCCCCCCCEECCCCCEEE
VTINGGYAMRAWYDIISSDFSQRREDPRGVRESAQQLENLIARENERGVSDANIVLAGFS
EEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECC
QGGAVALHTGLRHRRRLAGILALSTYLPLAETLAAEADPVNRDLPIFMAHGEADTVIPHA
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHHHH
FARRSAELLQASGYPLEWHNYPVDHTVSADEIRDLDAWLRRVLAPAG
HHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PNRILPAIEIETGAQPTHSVIWLHGLGADGHDFEPIVGELDTARLPPTRFLFPHAPIRT
CCCCCCEEEECCCCCCCCEEEEEEECCCCCCCCCHHHCCCCCCCCCCCCEECCCCCEEE
VTINGGYAMRAWYDIISSDFSQRREDPRGVRESAQQLENLIARENERGVSDANIVLAGFS
EEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECC
QGGAVALHTGLRHRRRLAGILALSTYLPLAETLAAEADPVNRDLPIFMAHGEADTVIPHA
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHHHH
FARRSAELLQASGYPLEWHNYPVDHTVSADEIRDLDAWLRRVLAPAG
HHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA