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The map label for this gene is pmbA [H]

Identifier: 119899084

GI number: 119899084

Start: 3076001

End: 3077341

Strand: Direct

Name: pmbA [H]

Synonym: azo2794

Alternate gene names: 119899084

Gene position: 3076001-3077341 (Clockwise)

Preceding gene: 119899081

Following gene: 119899085

Centisome position: 70.29

GC content: 67.04

Gene sequence:

>1341_bases
ATGTCCGCTCAAGGCTTCTCCTTCTCCCAGGCGCAGCTGCGCGAAATCGCCGCCGACATCCTCAAGTACGCCCGGAAGCG
CGGTGCGACCGCCTGCGAAACCGACGTGTCGGAAGGGTTCGGCCAATCGGTCGCGGTGCGCAAGGGCGAAGTCGATACCA
TCGAGTACAACCGCGACAAAGGCATCGGCGTCACCATCTACCTGGGCCAGCAGCGCGGCTATGCCAGTACCTCCGATTTT
TCCAAGGCGGCGCTGAAGGCCACCGTGGATGCCGCGGTGTCGATCGCCGGTTTCACTGCGCCCGACCCCTGCGCCGGCCT
GGCCGACGCCAGCCTGATGGCGCAGGACATGCCGGACCTCGACCTCTTCCATCCGTGGAAGATCGGCGTCGATGATGCGA
TCGGCCTCGCACGGCGCTGCGAAGAGGCCGCGTTCGCGGTGTCGCCCAAGATCACCAACTCCGAAGGCGCCAACGTTTCC
ATCCAGCAGTCGCAGTTCGTGTCGGCCAACAGCCACGGCTTCATGGGCGGCTACGCCACCACGCGGCACGGCCTGTCGTG
CTCCGTCATCGCGGGCGAAGGCGATGGCATGCAGCGCGAATACTGGTACGACTCGCGCCGCGACGCGGCGGACCTGATCA
GCCCCGAAGAAGTCGGCCGCATCGCCGGCGAACGCGCGATCAGCCGCCTGGGCGCCAAGAAGATCCGCACCTGCGAGGTG
CCGGTCATCTTCGAAGCGCCGCTCGCGATCGCCCTGCTCGGCAACTTCGTGCAGGCGGTGTCCGGCGGTTCGCTCTACCG
CAAGTCCAGCTTCCTGCTCGACAGCCTCGGCCAGCAGGTGTTTTCGCCGGTGGTCACCATCGCCGAACGGCCGCACCTGA
AGAAGGCCTTCGGCACCAGCTGGTTCGACAGCGACGGCGTCGCCACGCGCGACCGCGACGTGGTGGTGGACGGCGTGCTG
CAGGGCTACTTCCTGTCGACCTACTCCGCGCGCAAGCTGGGTATGCAGACGACCGGTAACGCCGGCGGTTCGCACAACCT
CACGGTCACGCCGGGCACCGACGACTTCGCCGGCCTCATCAAGCGCATGGAGCGCGGCCTGATCGTCACCGAGCTGCTCG
GCCACGGGGTCAACTACGTCACCGGCGACTACTCCCGCGGCGCGGCCGGCTACTGGGTGGAAAAGGGCAAGATCAAGCAC
GCGGTCGAGGAAGTCACCATCGCCGGCAACCTGCGCGACATGTTCCGCAACATCGTCGCGGTGGGCAACGACGCACTGCC
GCGCGGCGCCAAGCACTGCGGCTCGCTGCTGATCGAACGCATGAAGATCGCGGGCCGCTGA

Upstream 100 bases:

>100_bases
GTCGGCGGGGTCGTGATATTCAGGGCGGGAAACCATTGTGGGTCAAGCGTCAGGCGGGATCGGGGTAAGATTATAGCCTT
TGCCTCCCGGTGTTCCGCCC

Downstream 100 bases:

>100_bases
GCGGCCGGCACCTTCCCCGTCTCCGCACCTTCCCGGCCCGCCCGACCGCCCCCATCCGGGACGGTGGCGGGCCGTTCCAC
ATCCGCCGCCACTACGCCTT

Product: PmbA protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MSAQGFSFSQAQLREIAADILKYARKRGATACETDVSEGFGQSVAVRKGEVDTIEYNRDKGIGVTIYLGQQRGYASTSDF
SKAALKATVDAAVSIAGFTAPDPCAGLADASLMAQDMPDLDLFHPWKIGVDDAIGLARRCEEAAFAVSPKITNSEGANVS
IQQSQFVSANSHGFMGGYATTRHGLSCSVIAGEGDGMQREYWYDSRRDAADLISPEEVGRIAGERAISRLGAKKIRTCEV
PVIFEAPLAIALLGNFVQAVSGGSLYRKSSFLLDSLGQQVFSPVVTIAERPHLKKAFGTSWFDSDGVATRDRDVVVDGVL
QGYFLSTYSARKLGMQTTGNAGGSHNLTVTPGTDDFAGLIKRMERGLIVTELLGHGVNYVTGDYSRGAAGYWVEKGKIKH
AVEEVTIAGNLRDMFRNIVAVGNDALPRGAKHCGSLLIERMKIAGR

Sequences:

>Translated_446_residues
MSAQGFSFSQAQLREIAADILKYARKRGATACETDVSEGFGQSVAVRKGEVDTIEYNRDKGIGVTIYLGQQRGYASTSDF
SKAALKATVDAAVSIAGFTAPDPCAGLADASLMAQDMPDLDLFHPWKIGVDDAIGLARRCEEAAFAVSPKITNSEGANVS
IQQSQFVSANSHGFMGGYATTRHGLSCSVIAGEGDGMQREYWYDSRRDAADLISPEEVGRIAGERAISRLGAKKIRTCEV
PVIFEAPLAIALLGNFVQAVSGGSLYRKSSFLLDSLGQQVFSPVVTIAERPHLKKAFGTSWFDSDGVATRDRDVVVDGVL
QGYFLSTYSARKLGMQTTGNAGGSHNLTVTPGTDDFAGLIKRMERGLIVTELLGHGVNYVTGDYSRGAAGYWVEKGKIKH
AVEEVTIAGNLRDMFRNIVAVGNDALPRGAKHCGSLLIERMKIAGR
>Mature_445_residues
SAQGFSFSQAQLREIAADILKYARKRGATACETDVSEGFGQSVAVRKGEVDTIEYNRDKGIGVTIYLGQQRGYASTSDFS
KAALKATVDAAVSIAGFTAPDPCAGLADASLMAQDMPDLDLFHPWKIGVDDAIGLARRCEEAAFAVSPKITNSEGANVSI
QQSQFVSANSHGFMGGYATTRHGLSCSVIAGEGDGMQREYWYDSRRDAADLISPEEVGRIAGERAISRLGAKKIRTCEVP
VIFEAPLAIALLGNFVQAVSGGSLYRKSSFLLDSLGQQVFSPVVTIAERPHLKKAFGTSWFDSDGVATRDRDVVVDGVLQ
GYFLSTYSARKLGMQTTGNAGGSHNLTVTPGTDDFAGLIKRMERGLIVTELLGHGVNYVTGDYSRGAAGYWVEKGKIKHA
VEEVTIAGNLRDMFRNIVAVGNDALPRGAKHCGSLLIERMKIAGR

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=434, Percent_Identity=48.6175115207373, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI1789640, Length=402, Percent_Identity=22.8855721393035, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 47712; Mature: 47581

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAQGFSFSQAQLREIAADILKYARKRGATACETDVSEGFGQSVAVRKGEVDTIEYNRDK
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHCCCCCEEEECCCCCEEEECCCC
GIGVTIYLGQQRGYASTSDFSKAALKATVDAAVSIAGFTAPDPCAGLADASLMAQDMPDL
CCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCC
DLFHPWKIGVDDAIGLARRCEEAAFAVSPKITNSEGANVSIQQSQFVSANSHGFMGGYAT
CCCCCCCCCCHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEEHHHEECCCCCCCCCCCHH
TRHGLSCSVIAGEGDGMQREYWYDSRRDAADLISPEEVGRIAGERAISRLGAKKIRTCEV
HCCCCEEEEEECCCCCCCHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHCHHHCEECCC
PVIFEAPLAIALLGNFVQAVSGGSLYRKSSFLLDSLGQQVFSPVVTIAERPHLKKAFGTS
CEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCC
WFDSDGVATRDRDVVVDGVLQGYFLSTYSARKLGMQTTGNAGGSHNLTVTPGTDDFAGLI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCHHHHHH
KRMERGLIVTELLGHGVNYVTGDYSRGAAGYWVEKGKIKHAVEEVTIAGNLRDMFRNIVA
HHHHCCHHHHHHHHCCCCEEECCCCCCCCCEEEECCHHHHHHHHHHHCCCHHHHHHHHHH
VGNDALPRGAKHCGSLLIERMKIAGR
HCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SAQGFSFSQAQLREIAADILKYARKRGATACETDVSEGFGQSVAVRKGEVDTIEYNRDK
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHCCCCCEEEECCCCCEEEECCCC
GIGVTIYLGQQRGYASTSDFSKAALKATVDAAVSIAGFTAPDPCAGLADASLMAQDMPDL
CCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCC
DLFHPWKIGVDDAIGLARRCEEAAFAVSPKITNSEGANVSIQQSQFVSANSHGFMGGYAT
CCCCCCCCCCHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEEHHHEECCCCCCCCCCCHH
TRHGLSCSVIAGEGDGMQREYWYDSRRDAADLISPEEVGRIAGERAISRLGAKKIRTCEV
HCCCCEEEEEECCCCCCCHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHCHHHCEECCC
PVIFEAPLAIALLGNFVQAVSGGSLYRKSSFLLDSLGQQVFSPVVTIAERPHLKKAFGTS
CEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCC
WFDSDGVATRDRDVVVDGVLQGYFLSTYSARKLGMQTTGNAGGSHNLTVTPGTDDFAGLI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCHHHHHH
KRMERGLIVTELLGHGVNYVTGDYSRGAAGYWVEKGKIKHAVEEVTIAGNLRDMFRNIVA
HHHHCCHHHHHHHHCCCCEEECCCCCCCCCEEEECCHHHHHHHHHHHCCCHHHHHHHHHH
VGNDALPRGAKHCGSLLIERMKIAGR
HCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]