Click here to switch to the map view.

The map label for this gene is ilvE2 [H]

Identifier: 119899065

GI number: 119899065

Start: 3055412

End: 3056332

Strand: Direct

Name: ilvE2 [H]

Synonym: azo2775

Alternate gene names: 119899065

Gene position: 3055412-3056332 (Clockwise)

Preceding gene: 119899061

Following gene: 119899066

Centisome position: 69.82

GC content: 65.26

Gene sequence:

>921_bases
ATGTCCATGGCCGACCGCGACGGTTTCATCTGGTACGACGGCAAGCTCGTACCGTGGCGCGACGCAACCACCCACGTCCT
CACCCACTCCCTGCACTACGGGCTGTCGGTGTTCGAGGGCGTGCGCGCCTACAAGACCGCCAAGGGCACCGCGATCTTCC
GCCTCGCCGAGCACACCCAGCGTCTGCTCAACTCCGGCAAGATCTACATGATGGACATCCCCTACTCCAAGGAAGTCCTG
ATGGAAGCGCAGAAGGAAGTCGTGCGCGCCAACAAGCTGGAATCCTGCTACCTGCGCCCGATCGCCTTCTACGGCTCGGA
AAAGATGGGCATCTCCACCCGCGGCGCCACGGTGCACGTCGCCATCGCGGCCTGGCCCTGGGGCGCCTACCTGGGCGAAG
ACGGCCTGGAGAAAGGCATCCGCGTCAAGACCTCGTCCTTCGCCCGCCAGCACGTCAACGTGACCATGGCACGCGCCAAG
CTCGCCAGCACCTACGCCAACTCCATCCTCGCCAACCTCGAGGCCACCCAGGACGGCTACGACGAGGCGCTGCTGCTCGA
CACCCAGGGCTTTGTCGCCGAAGGCTCCGGCGAGAACCTCTTCGTCATCAAGGACGGCGTGATCTACGAACCCGAGATCG
CCTCCGCGCTGACCGGCATCACCCGCGACTCCGTCATCCGCGTCGCGCGCGAACTCGGCTACGAAGTGCAGTCGCGCCGC
ATGACCCGCGACGACATCTACATCGCCGACGAAGCCTTCTTCACCGGCACCGCGGCGGAAGTGACGCCGATCCGCGAACT
CGACAACCGTACCATCGGCGAAGGCCGCCGCGGCCCGATCACCACCAAGGTGCAGGCGCGCTTCTTCGACATTGTCAATG
GCCGCGCCCCCGAGTACGAAAACTGGCTGTCCCTGGTCTGA

Upstream 100 bases:

>100_bases
GTTTGCAGGCCGCGACCGGCCCTTTCCTTTGCGATAGAATTCGGCAGTTAAGCCCGGCCGCCGGGCAGATTCGAACTCAT
TTCCGCTGGAGATTCCCACC

Downstream 100 bases:

>100_bases
GGAATCCGAGATGCCCGAAAACAAAGACAAGTCGCAACCCATCGCCGTCAGCGCGCACGACCTGCCGCTGTACTGTCCGC
AGCCCGATGCGCCGCTGTGG

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MSMADRDGFIWYDGKLVPWRDATTHVLTHSLHYGLSVFEGVRAYKTAKGTAIFRLAEHTQRLLNSGKIYMMDIPYSKEVL
MEAQKEVVRANKLESCYLRPIAFYGSEKMGISTRGATVHVAIAAWPWGAYLGEDGLEKGIRVKTSSFARQHVNVTMARAK
LASTYANSILANLEATQDGYDEALLLDTQGFVAEGSGENLFVIKDGVIYEPEIASALTGITRDSVIRVARELGYEVQSRR
MTRDDIYIADEAFFTGTAAEVTPIRELDNRTIGEGRRGPITTKVQARFFDIVNGRAPEYENWLSLV

Sequences:

>Translated_306_residues
MSMADRDGFIWYDGKLVPWRDATTHVLTHSLHYGLSVFEGVRAYKTAKGTAIFRLAEHTQRLLNSGKIYMMDIPYSKEVL
MEAQKEVVRANKLESCYLRPIAFYGSEKMGISTRGATVHVAIAAWPWGAYLGEDGLEKGIRVKTSSFARQHVNVTMARAK
LASTYANSILANLEATQDGYDEALLLDTQGFVAEGSGENLFVIKDGVIYEPEIASALTGITRDSVIRVARELGYEVQSRR
MTRDDIYIADEAFFTGTAAEVTPIRELDNRTIGEGRRGPITTKVQARFFDIVNGRAPEYENWLSLV
>Mature_305_residues
SMADRDGFIWYDGKLVPWRDATTHVLTHSLHYGLSVFEGVRAYKTAKGTAIFRLAEHTQRLLNSGKIYMMDIPYSKEVLM
EAQKEVVRANKLESCYLRPIAFYGSEKMGISTRGATVHVAIAAWPWGAYLGEDGLEKGIRVKTSSFARQHVNVTMARAKL
ASTYANSILANLEATQDGYDEALLLDTQGFVAEGSGENLFVIKDGVIYEPEIASALTGITRDSVIRVARELGYEVQSRRM
TRDDIYIADEAFFTGTAAEVTPIRELDNRTIGEGRRGPITTKVQARFFDIVNGRAPEYENWLSLV

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI50658084, Length=292, Percent_Identity=26.027397260274, Blast_Score=79, Evalue=8e-15,
Organism=Escherichia coli, GI48994963, Length=300, Percent_Identity=46.6666666666667, Blast_Score=297, Evalue=6e-82,
Organism=Caenorhabditis elegans, GI17565728, Length=279, Percent_Identity=24.0143369175627, Blast_Score=71, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6322002, Length=329, Percent_Identity=27.355623100304, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6322608, Length=328, Percent_Identity=25.3048780487805, Blast_Score=85, Evalue=2e-17,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 34164; Mature: 34033

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMADRDGFIWYDGKLVPWRDATTHVLTHSLHYGLSVFEGVRAYKTAKGTAIFRLAEHTQ
CCCCCCCCEEEECCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHH
RLLNSGKIYMMDIPYSKEVLMEAQKEVVRANKLESCYLRPIAFYGSEKMGISTRGATVHV
HHHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCCHHHCCEEEEECCCCCCEEECCCEEEE
AIAAWPWGAYLGEDGLEKGIRVKTSSFARQHVNVTMARAKLASTYANSILANLEATQDGY
EEEECCCHHHCCCHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DEALLLDTQGFVAEGSGENLFVIKDGVIYEPEIASALTGITRDSVIRVARELGYEVQSRR
CCEEEEECCCCEEECCCCEEEEEECCEEECCHHHHHHHCCCHHHHHHHHHHHCCHHHHCC
MTRDDIYIADEAFFTGTAAEVTPIRELDNRTIGEGRRGPITTKVQARFFDIVNGRAPEYE
CCCCCEEEECCCEECCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHH
NWLSLV
HHHHCC
>Mature Secondary Structure 
SMADRDGFIWYDGKLVPWRDATTHVLTHSLHYGLSVFEGVRAYKTAKGTAIFRLAEHTQ
CCCCCCCEEEECCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHH
RLLNSGKIYMMDIPYSKEVLMEAQKEVVRANKLESCYLRPIAFYGSEKMGISTRGATVHV
HHHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCCHHHCCEEEEECCCCCCEEECCCEEEE
AIAAWPWGAYLGEDGLEKGIRVKTSSFARQHVNVTMARAKLASTYANSILANLEATQDGY
EEEECCCHHHCCCHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DEALLLDTQGFVAEGSGENLFVIKDGVIYEPEIASALTGITRDSVIRVARELGYEVQSRR
CCEEEEECCCCEEECCCCEEEEEECCEEECCHHHHHHHCCCHHHHHHHHHHHCCHHHHCC
MTRDDIYIADEAFFTGTAAEVTPIRELDNRTIGEGRRGPITTKVQARFFDIVNGRAPEYE
CCCCCEEEECCCEECCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHH
NWLSLV
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]