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The map label for this gene is yfhA [H]

Identifier: 119899060

GI number: 119899060

Start: 3050477

End: 3051874

Strand: Direct

Name: yfhA [H]

Synonym: azo2770

Alternate gene names: 119899060

Gene position: 3050477-3051874 (Clockwise)

Preceding gene: 119899059

Following gene: 119899061

Centisome position: 69.71

GC content: 69.24

Gene sequence:

>1398_bases
ATGACGCCGACCGAGCACCGCCATCTGCTGGTGGTCGATGACGACCCCGACCTGCTGCGCCTGCTGGCGATGCGGCTGCA
GGCCAACGGCTATCGCGTCAGCCCGGTGGGCACCGCCGAAGCAGCACTCGCACGTCTTGCGGTGGAACGGGTGGACCTGG
TGTTGAGCGATGTCCGCCTGCCCGACCGCGACGGCCTGGCTCTGTTCGAAGAGGTACGGCGCCTGTACCCCGCACTGCCG
GTGATCCTGCTCACGGCGCACGGCACCATCCCGGACGCGATCGATGCGACCTCGCGTGGCGTCGCCGGCTACCTGACCAA
GCCCTACGACAGCCAGGACCTGCTGGACAACGTGGAGCGCGCGCTGCATCTGGCCGCGCCGGCCGGCGATACGGGCAGCG
GCACCACCACCGAGGCCTACGCATTCGAAGGCATGCTCAGCCGCAGCACGCGCATGCACGCGCTGTTCGACGAAACCCGG
CTGGTCGCGGCCTCCGACGCCAGCGTGCTGATCCGCGGCGAGAGCGGCACCGGCAAGGAAGTGCTCGCGCACGCCATCCA
TGCCGCCAGCCCGCGCGCCTCGGCGCCCTTCGTTGCGGTCAATTGCGGCGCGATTCCGGAAGCGCTGCTCGAATCGGAAC
TGTTCGGTCACGTCCGCGGCGCCTTCACCGGCGCGGTAACCAATCGCCGCGGCCTCTTCCAGGCGGCCCATGGCGGCACC
TTGTTCCTGGACGAGATCGGCGACATGCCGCTGACGCTGCAGGTGAAACTGCTGCGCGTGCTGCAGGAGCGCGCGGTGCG
CCCCGTGGGCTCGACCCGCAGCGAGCCGATCGACGTCCGCATCGTCTCGGCCACCCACCGCGATCTCGACAGCGAACTCA
CCGCCGGCCGGTTTCGTGAAGACCTGTATTACCGGATCAACGTGGTCAACCTCAACCTGCCGACGCTGAGCGAACGGCGC
GAGGACATTCCTCTGCTTGCCGAGCACTTCCTGCGCGAACAGGCGGCGCGCTACGGGAAACCCCTGCGCGGCTTCGCCCC
CGGCGCACTGGAAGCGCTGGTCACCGCCAGCTGGCCGGGCAACATCCGCCAGTTGCAGAACGTGGTGGAGCAGACCTGCG
CCCTCGCCACGACGCCACTGATTCCGCTCGCGCTGGTCAAACGCGCGCTGCGCGCAACGCCGCCCGCGGCACTGAGCTAT
GCCGAGGCCAAGCAGCGTTTCGAACGCGGCTATCTCGTCCAGTTGCTCAAGCTCACCGAGGGCCGCGTTGCCGATGCTGC
CCGCCTGGCGCAACGCAACCGCACCGAGTTCTACCGCCTCATCCAGCGCCACGGCCTGGATCCGGCGCTGTTCCGCGGCC
CCGCATCGCCGCGCGAATCCGCTGACGACGGCGCTTGA

Upstream 100 bases:

>100_bases
ATAGCGACCTGCAGGGCAAGCTCGACGCGCTCAGCGAAATCGAGCGCAGCCTGTCCGGCGGCGCAACGCGGCCCCCCGTC
CCGGAACCGGACCGCCCCCG

Downstream 100 bases:

>100_bases
CCCACCGCGCCGAGGAGGAGGAAACTCGGCGCATGACTGTACAAAAACACAGCTCTCTCAACAGTGCCGTCGCTGCCGGG
CCACGCGGCCGCGGCAGCGC

Product: putative two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MTPTEHRHLLVVDDDPDLLRLLAMRLQANGYRVSPVGTAEAALARLAVERVDLVLSDVRLPDRDGLALFEEVRRLYPALP
VILLTAHGTIPDAIDATSRGVAGYLTKPYDSQDLLDNVERALHLAAPAGDTGSGTTTEAYAFEGMLSRSTRMHALFDETR
LVAASDASVLIRGESGTGKEVLAHAIHAASPRASAPFVAVNCGAIPEALLESELFGHVRGAFTGAVTNRRGLFQAAHGGT
LFLDEIGDMPLTLQVKLLRVLQERAVRPVGSTRSEPIDVRIVSATHRDLDSELTAGRFREDLYYRINVVNLNLPTLSERR
EDIPLLAEHFLREQAARYGKPLRGFAPGALEALVTASWPGNIRQLQNVVEQTCALATTPLIPLALVKRALRATPPAALSY
AEAKQRFERGYLVQLLKLTEGRVADAARLAQRNRTEFYRLIQRHGLDPALFRGPASPRESADDGA

Sequences:

>Translated_465_residues
MTPTEHRHLLVVDDDPDLLRLLAMRLQANGYRVSPVGTAEAALARLAVERVDLVLSDVRLPDRDGLALFEEVRRLYPALP
VILLTAHGTIPDAIDATSRGVAGYLTKPYDSQDLLDNVERALHLAAPAGDTGSGTTTEAYAFEGMLSRSTRMHALFDETR
LVAASDASVLIRGESGTGKEVLAHAIHAASPRASAPFVAVNCGAIPEALLESELFGHVRGAFTGAVTNRRGLFQAAHGGT
LFLDEIGDMPLTLQVKLLRVLQERAVRPVGSTRSEPIDVRIVSATHRDLDSELTAGRFREDLYYRINVVNLNLPTLSERR
EDIPLLAEHFLREQAARYGKPLRGFAPGALEALVTASWPGNIRQLQNVVEQTCALATTPLIPLALVKRALRATPPAALSY
AEAKQRFERGYLVQLLKLTEGRVADAARLAQRNRTEFYRLIQRHGLDPALFRGPASPRESADDGA
>Mature_464_residues
TPTEHRHLLVVDDDPDLLRLLAMRLQANGYRVSPVGTAEAALARLAVERVDLVLSDVRLPDRDGLALFEEVRRLYPALPV
ILLTAHGTIPDAIDATSRGVAGYLTKPYDSQDLLDNVERALHLAAPAGDTGSGTTTEAYAFEGMLSRSTRMHALFDETRL
VAASDASVLIRGESGTGKEVLAHAIHAASPRASAPFVAVNCGAIPEALLESELFGHVRGAFTGAVTNRRGLFQAAHGGTL
FLDEIGDMPLTLQVKLLRVLQERAVRPVGSTRSEPIDVRIVSATHRDLDSELTAGRFREDLYYRINVVNLNLPTLSERRE
DIPLLAEHFLREQAARYGKPLRGFAPGALEALVTASWPGNIRQLQNVVEQTCALATTPLIPLALVKRALRATPPAALSYA
EAKQRFERGYLVQLLKLTEGRVADAARLAQRNRTEFYRLIQRHGLDPALFRGPASPRESADDGA

Specific function: Probable member of a two-component regulatory system yfhA/yfhK [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788905, Length=445, Percent_Identity=57.0786516853933, Blast_Score=490, Evalue=1e-140,
Organism=Escherichia coli, GI1790437, Length=429, Percent_Identity=45.2214452214452, Blast_Score=351, Evalue=5e-98,
Organism=Escherichia coli, GI1788550, Length=455, Percent_Identity=40.4395604395604, Blast_Score=332, Evalue=3e-92,
Organism=Escherichia coli, GI1790299, Length=472, Percent_Identity=40.0423728813559, Blast_Score=313, Evalue=1e-86,
Organism=Escherichia coli, GI87082117, Length=319, Percent_Identity=45.7680250783699, Blast_Score=259, Evalue=3e-70,
Organism=Escherichia coli, GI1789087, Length=314, Percent_Identity=44.5859872611465, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI1789233, Length=210, Percent_Identity=54.7619047619048, Blast_Score=238, Evalue=6e-64,
Organism=Escherichia coli, GI87082152, Length=331, Percent_Identity=42.5981873111783, Blast_Score=237, Evalue=9e-64,
Organism=Escherichia coli, GI1786524, Length=245, Percent_Identity=49.7959183673469, Blast_Score=222, Evalue=5e-59,
Organism=Escherichia coli, GI1787583, Length=284, Percent_Identity=39.0845070422535, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI87081872, Length=263, Percent_Identity=40.3041825095057, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1789828, Length=254, Percent_Identity=37.007874015748, Blast_Score=151, Evalue=9e-38,
Organism=Escherichia coli, GI87081858, Length=299, Percent_Identity=31.438127090301, Blast_Score=145, Evalue=7e-36,
Organism=Escherichia coli, GI1790552, Length=106, Percent_Identity=37.7358490566038, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI145693157, Length=118, Percent_Identity=36.4406779661017, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789809, Length=122, Percent_Identity=33.6065573770492, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1786911, Length=125, Percent_Identity=33.6, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50692; Mature: 50560

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPTEHRHLLVVDDDPDLLRLLAMRLQANGYRVSPVGTAEAALARLAVERVDLVLSDVRL
CCCCCCCEEEEECCCHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCC
PDRDGLALFEEVRRLYPALPVILLTAHGTIPDAIDATSRGVAGYLTKPYDSQDLLDNVER
CCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHCCCCCEECCCCCCCHHHHHHHHHH
ALHLAAPAGDTGSGTTTEAYAFEGMLSRSTRMHALFDETRLVAASDASVLIRGESGTGKE
HHHHCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCEEEEECCCEEEEECCCCCHHH
VLAHAIHAASPRASAPFVAVNCGAIPEALLESELFGHVRGAFTGAVTNRRGLFQAAHGGT
HHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCE
LFLDEIGDMPLTLQVKLLRVLQERAVRPVGSTRSEPIDVRIVSATHRDLDSELTAGRFRE
EEEHHHCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECHHHCHHHHHHHHHHHH
DLYYRINVVNLNLPTLSERREDIPLLAEHFLREQAARYGKPLRGFAPGALEALVTASWPG
CEEEEEEEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHEECCCCC
NIRQLQNVVEQTCALATTPLIPLALVKRALRATPPAALSYAEAKQRFERGYLVQLLKLTE
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GRVADAARLAQRNRTEFYRLIQRHGLDPALFRGPASPRESADDGA
CCHHHHHHHHHCCHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TPTEHRHLLVVDDDPDLLRLLAMRLQANGYRVSPVGTAEAALARLAVERVDLVLSDVRL
CCCCCCEEEEECCCHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCC
PDRDGLALFEEVRRLYPALPVILLTAHGTIPDAIDATSRGVAGYLTKPYDSQDLLDNVER
CCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHCCCCCEECCCCCCCHHHHHHHHHH
ALHLAAPAGDTGSGTTTEAYAFEGMLSRSTRMHALFDETRLVAASDASVLIRGESGTGKE
HHHHCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCEEEEECCCEEEEECCCCCHHH
VLAHAIHAASPRASAPFVAVNCGAIPEALLESELFGHVRGAFTGAVTNRRGLFQAAHGGT
HHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCE
LFLDEIGDMPLTLQVKLLRVLQERAVRPVGSTRSEPIDVRIVSATHRDLDSELTAGRFRE
EEEHHHCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECHHHCHHHHHHHHHHHH
DLYYRINVVNLNLPTLSERREDIPLLAEHFLREQAARYGKPLRGFAPGALEALVTASWPG
CEEEEEEEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHEECCCCC
NIRQLQNVVEQTCALATTPLIPLALVKRALRATPPAALSYAEAKQRFERGYLVQLLKLTE
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GRVADAARLAQRNRTEFYRLIQRHGLDPALFRGPASPRESADDGA
CCHHHHHHHHHCCHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]