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The map label for this gene is proS

Identifier: 119899055

GI number: 119899055

Start: 3045152

End: 3046897

Strand: Direct

Name: proS

Synonym: azo2765

Alternate gene names: 119899055

Gene position: 3045152-3046897 (Clockwise)

Preceding gene: 119899051

Following gene: 119899056

Centisome position: 69.59

GC content: 65.98

Gene sequence:

>1746_bases
ATGCGCGCACGTCAATTCTTCATCTCTACCCTCAAGGAAGCCCCCGCGGACGCGGAAGTCGTCAGCCAGAAACTGATGCT
GCGCGCGGGCATGATCCGCAAGGTCGCCGCCGGCATCTACAACTACCTGCCGATGGGCCTGAAGAGCATCCGCAAGGTGG
AAAACATCATCCGCGAGGAAATGAACCGCGCCGGCGCGCTCGAACTCACCATGCCGCTGGTGCAGCCGTCGGAGCTGTGG
CAGGAAACCGGCCGCTGGGACAAGATGGGCCCCGAGATGCTGCGTTTCAAGGATCGCCACGAGCGCGATTTCGCACTGCA
GCCCACCTCCGAGGAAGTCGTTACCGACATCGCCCGCCAGGAGCTGAAGAGCTACCGCCAGCTGCCCAAGAACTTCTACC
AGATCCAGACCAAGTTCCGCGACGAGCGCCGGCCGCGCTTTGGCGTGATGCGCGGACGTGAATTCACCATGAAGGACGCC
TACTCCTTCGACCGCAGCGCGGAAGCGGCCGGTGAAAGCTACGACGCGATGTATGCCGCCTACAGCCGCATCTTCGATCG
CCTCGGCCTCACCTACCGGGCGGTCGCGGCCGACACCGGCGCCATCGGCGGCGACCGTTCGCACGAATTCCAGGTCATCG
CCGACACTGGCGAGGACGCCATCGTCTACTGCCCCGATTCCAGCTACGCGGCCAACATCGAACTCGCCGAAGCGCTGTCG
CTGCTTCCGGCGCGCGCGGGCGCGTCCGCCCCGCTGGCAAAGACACCGACGCCGGGCAAGTCGACCTGCGAGGATGTCGC
CGCGCTGCTCGGCGTGCCGCTTGCCAGCACGGTGAAATCGCTGGTGCTCGCCACCGACGACGAGGACGAGAGCGGCAAGG
TGGTGAAGAGCACCGTGTGGCTGCTGCTGGTGCGTGGCGACCACGAACTCAACGAAGTGAAGGCAGGGAAGATCGAAGGA
CTGAAGGCCGGGTTCCGCTTCGCGACCGAGGCGGAAATCCTCGACCATTTCGGCTGCAAGCCGGGCTACCTCGGTCCGAT
CGGCCTCGCCCAGCCGGTCAAGGTGATTGCCGACCGCACCGTCGCCAACATGGCCGACTTCGTATGCGGGGCCAACGCCG
AGGACTTCCACTACACCGGCGCCAACTGGGGCCGCGACCTGCCGGAGCCGGACCTCGTGGCCGACATCCGCAACGTGGTC
GAAGGCGATCCCAGCCCGGACGGCAAGGGCAAGCTGGCGATCCAGCGCGGCATCGAGGTCGGGCATGTGTTCTACCTCGG
CACCAAGTACTCGAAGGCGATGAACGCCACCTTCCTCGACGTCGACGGCAAGCCCAAGCACTTCGAGATGGGCTGCTACG
GCATCGGCGTCACCCGCATCCTCGGCGCCGCCATCGAGCAGAACCACGACGAGCGCGGCATCATCTGGCCCGCCTCCATC
GCGCCGTTCGAAGTGGTGATCTGCCCGGTGGGCTGGAGCAAGTCGGAAGCGGTGCGCGACGAAGCGCAGAAGCTTTACGA
CGCGCTCGTGGCTGCGGGTGTCGATGTGATCCTGGACGATCGTGACGAACGTCCCGGGGTCATGTTTGCCGACTGGGAGC
TGATCGGCGTGCCCCACCGCGTGACCATCGGCGACCGTGGCCTGAAGGAAGGCGTCGCCGAGTACCAGGGCCGCCGTGAT
CCCGAAGCCAGCCGCGTCGCGGTCGCGGAGATCGCGCCGCATTTGATTTCCCGGCTGCGCCCCTGA

Upstream 100 bases:

>100_bases
CTTGGAAAGCGCGCCGGGAGGGCGGTTCCGCGCCGCAGCGGCCGTTGGCGCCGGCGGTGCGCTAGAATCGGCGGCTATTT
CAACGCAACACCCGGACAGC

Downstream 100 bases:

>100_bases
GCCTGTACGCCGGGAATCGCCACATGCTCCGCGCCCTGCTCCTGCCCCCACGGCTGCGCCGGTTTGCCGTGGCGGCCGTA
CTGGCCGCAGGCTGTAGCGC

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 581; Mature: 581

Protein sequence:

>581_residues
MRARQFFISTLKEAPADAEVVSQKLMLRAGMIRKVAAGIYNYLPMGLKSIRKVENIIREEMNRAGALELTMPLVQPSELW
QETGRWDKMGPEMLRFKDRHERDFALQPTSEEVVTDIARQELKSYRQLPKNFYQIQTKFRDERRPRFGVMRGREFTMKDA
YSFDRSAEAAGESYDAMYAAYSRIFDRLGLTYRAVAADTGAIGGDRSHEFQVIADTGEDAIVYCPDSSYAANIELAEALS
LLPARAGASAPLAKTPTPGKSTCEDVAALLGVPLASTVKSLVLATDDEDESGKVVKSTVWLLLVRGDHELNEVKAGKIEG
LKAGFRFATEAEILDHFGCKPGYLGPIGLAQPVKVIADRTVANMADFVCGANAEDFHYTGANWGRDLPEPDLVADIRNVV
EGDPSPDGKGKLAIQRGIEVGHVFYLGTKYSKAMNATFLDVDGKPKHFEMGCYGIGVTRILGAAIEQNHDERGIIWPASI
APFEVVICPVGWSKSEAVRDEAQKLYDALVAAGVDVILDDRDERPGVMFADWELIGVPHRVTIGDRGLKEGVAEYQGRRD
PEASRVAVAEIAPHLISRLRP

Sequences:

>Translated_581_residues
MRARQFFISTLKEAPADAEVVSQKLMLRAGMIRKVAAGIYNYLPMGLKSIRKVENIIREEMNRAGALELTMPLVQPSELW
QETGRWDKMGPEMLRFKDRHERDFALQPTSEEVVTDIARQELKSYRQLPKNFYQIQTKFRDERRPRFGVMRGREFTMKDA
YSFDRSAEAAGESYDAMYAAYSRIFDRLGLTYRAVAADTGAIGGDRSHEFQVIADTGEDAIVYCPDSSYAANIELAEALS
LLPARAGASAPLAKTPTPGKSTCEDVAALLGVPLASTVKSLVLATDDEDESGKVVKSTVWLLLVRGDHELNEVKAGKIEG
LKAGFRFATEAEILDHFGCKPGYLGPIGLAQPVKVIADRTVANMADFVCGANAEDFHYTGANWGRDLPEPDLVADIRNVV
EGDPSPDGKGKLAIQRGIEVGHVFYLGTKYSKAMNATFLDVDGKPKHFEMGCYGIGVTRILGAAIEQNHDERGIIWPASI
APFEVVICPVGWSKSEAVRDEAQKLYDALVAAGVDVILDDRDERPGVMFADWELIGVPHRVTIGDRGLKEGVAEYQGRRD
PEASRVAVAEIAPHLISRLRP
>Mature_581_residues
MRARQFFISTLKEAPADAEVVSQKLMLRAGMIRKVAAGIYNYLPMGLKSIRKVENIIREEMNRAGALELTMPLVQPSELW
QETGRWDKMGPEMLRFKDRHERDFALQPTSEEVVTDIARQELKSYRQLPKNFYQIQTKFRDERRPRFGVMRGREFTMKDA
YSFDRSAEAAGESYDAMYAAYSRIFDRLGLTYRAVAADTGAIGGDRSHEFQVIADTGEDAIVYCPDSSYAANIELAEALS
LLPARAGASAPLAKTPTPGKSTCEDVAALLGVPLASTVKSLVLATDDEDESGKVVKSTVWLLLVRGDHELNEVKAGKIEG
LKAGFRFATEAEILDHFGCKPGYLGPIGLAQPVKVIADRTVANMADFVCGANAEDFHYTGANWGRDLPEPDLVADIRNVV
EGDPSPDGKGKLAIQRGIEVGHVFYLGTKYSKAMNATFLDVDGKPKHFEMGCYGIGVTRILGAAIEQNHDERGIIWPASI
APFEVVICPVGWSKSEAVRDEAQKLYDALVAAGVDVILDDRDERPGVMFADWELIGVPHRVTIGDRGLKEGVAEYQGRRD
PEASRVAVAEIAPHLISRLRP

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=250, Percent_Identity=45.6, Blast_Score=233, Evalue=4e-61,
Organism=Escherichia coli, GI1786392, Length=580, Percent_Identity=56.7241379310345, Blast_Score=680, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=238, Percent_Identity=38.2352941176471, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI193203271, Length=106, Percent_Identity=35.8490566037736, Blast_Score=87, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6320931, Length=219, Percent_Identity=36.5296803652968, Blast_Score=175, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24656200, Length=224, Percent_Identity=40.1785714285714, Blast_Score=186, Evalue=3e-47,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_AZOSB (A1K976)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_934268.1
- ProteinModelPortal:   A1K976
- SMR:   A1K976
- STRING:   A1K976
- GeneID:   4607605
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo2765
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- PhylomeDB:   A1K976
- ProtClustDB:   PRK09194
- BioCyc:   ASP62928:AZO2765-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 64026; Mature: 64026

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRARQFFISTLKEAPADAEVVSQKLMLRAGMIRKVAAGIYNYLPMGLKSIRKVENIIREE
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNRAGALELTMPLVQPSELWQETGRWDKMGPEMLRFKDRHERDFALQPTSEEVVTDIARQ
HHCCCCEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
ELKSYRQLPKNFYQIQTKFRDERRPRFGVMRGREFTMKDAYSFDRSAEAAGESYDAMYAA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCHHHCCCHHHHHHHH
YSRIFDRLGLTYRAVAADTGAIGGDRSHEFQVIADTGEDAIVYCPDSSYAANIELAEALS
HHHHHHHHCCEEEEEECCCCCCCCCCCCCEEEEEECCCCEEEECCCCCCCCCHHHHHHHH
LLPARAGASAPLAKTPTPGKSTCEDVAALLGVPLASTVKSLVLATDDEDESGKVVKSTVW
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHEEECCCCCCCCCEEEEEEE
LLLVRGDHELNEVKAGKIEGLKAGFRFATEAEILDHFGCKPGYLGPIGLAQPVKVIADRT
EEEEECCCCHHHHCCCCCCCHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
VANMADFVCGANAEDFHYTGANWGRDLPEPDLVADIRNVVEGDPSPDGKGKLAIQRGIEV
HHHHHHHHHCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEECCCCE
GHVFYLGTKYSKAMNATFLDVDGKPKHFEMGCYGIGVTRILGAAIEQNHDERGIIWPASI
EEEEEECCHHHHHCCCEEEECCCCCCEEECCEEHHHHHHHHHHHHHCCCCCCCEEEECCC
APFEVVICPVGWSKSEAVRDEAQKLYDALVAAGVDVILDDRDERPGVMFADWELIGVPHR
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEECEEEECCCEE
VTIGDRGLKEGVAEYQGRRDPEASRVAVAEIAPHLISRLRP
EEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRARQFFISTLKEAPADAEVVSQKLMLRAGMIRKVAAGIYNYLPMGLKSIRKVENIIREE
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNRAGALELTMPLVQPSELWQETGRWDKMGPEMLRFKDRHERDFALQPTSEEVVTDIARQ
HHCCCCEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
ELKSYRQLPKNFYQIQTKFRDERRPRFGVMRGREFTMKDAYSFDRSAEAAGESYDAMYAA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCHHHCCCHHHHHHHH
YSRIFDRLGLTYRAVAADTGAIGGDRSHEFQVIADTGEDAIVYCPDSSYAANIELAEALS
HHHHHHHHCCEEEEEECCCCCCCCCCCCCEEEEEECCCCEEEECCCCCCCCCHHHHHHHH
LLPARAGASAPLAKTPTPGKSTCEDVAALLGVPLASTVKSLVLATDDEDESGKVVKSTVW
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHEEECCCCCCCCCEEEEEEE
LLLVRGDHELNEVKAGKIEGLKAGFRFATEAEILDHFGCKPGYLGPIGLAQPVKVIADRT
EEEEECCCCHHHHCCCCCCCHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
VANMADFVCGANAEDFHYTGANWGRDLPEPDLVADIRNVVEGDPSPDGKGKLAIQRGIEV
HHHHHHHHHCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEECCCCE
GHVFYLGTKYSKAMNATFLDVDGKPKHFEMGCYGIGVTRILGAAIEQNHDERGIIWPASI
EEEEEECCHHHHHCCCEEEECCCCCCEEECCEEHHHHHHHHHHHHHCCCCCCCEEEECCC
APFEVVICPVGWSKSEAVRDEAQKLYDALVAAGVDVILDDRDERPGVMFADWELIGVPHR
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEECEEEECCCEE
VTIGDRGLKEGVAEYQGRRDPEASRVAVAEIAPHLISRLRP
EEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA