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The map label for this gene is flgJ [H]

Identifier: 119899022

GI number: 119899022

Start: 3015668

End: 3016735

Strand: Reverse

Name: flgJ [H]

Synonym: azo2732

Alternate gene names: 119899022

Gene position: 3016735-3015668 (Counterclockwise)

Preceding gene: 119899023

Following gene: 119899021

Centisome position: 68.94

GC content: 70.69

Gene sequence:

>1068_bases
ATGACCGCGGCGAACACCGGCTTCCAGCTCAACGCGCTCGATCCGAATTCGCTCGGCGACCTCAAGCGGCTCGCCCGCAA
CAACCCGGATTCGCCCGAAACCCTGCGCGCGGCCTCCAAGCAGTTCGAGGCCCTGTTCCTGCAGATGGCGCTCAAGTCGA
TGCGCGAGGCGACGCCGGGCTCCACGCTGTTCGACAACGAGCAGACCAAGAGCTATCAGTCGCTGCTCGACCAGCAGCTC
GCGCTCAACATGGCGCACAGCCGCAACAACGGCATGAGCGAGGCGCTGTTCCGCCAGCTCGGCGGCATCAACGGCAAGGG
GGCCGCCACCCCGGCGGTGGCGGAAGGTCTGCCGCTGCCCGGCGCGGCCGGCGCGAACCCGGGATTCGACATCTCCAACG
CGATCCGCCAGTCGTCCAGCCCGGCGGCACTGATGCGGCAGGCGCAGGCGATCGCGGCCGGCGCGGAAAGCCGCGGCGGG
GCGGACGGCGACAGCATCGGCGCGCTGATCTCGCGGCTGGAGAATGCGGTGCGCGCCACCCGCGACAAGGCGGCCGCGGG
GGCGACCGCGCCCGCGGACGGCAACGATGGCGTGGCCGACGACGTGCGCGAGTTCGTCAACGCGGTGTGGCCGCACGCGC
AGAGCGCCAGCCGGCAGACCGGCATTCCGGCGCAGTTCATCGTCGCCCAGGCGGCGCTCGAAACCGGCTGGGGCGACAAG
GTGCTGCGCCATGCCGACGGCCGCAACAGCTACAACCTCTTCAACATCAAGGCCGGTGCCGGCTGGACGGGCGACACGGT
GACGCGCAAGGTCACCGAGTACAGCGGCGGCAGCGCCTACACCGAACAGGCCCGTTTCCGCAGCTACGGCTCGTACGCGG
AAGCCTTCCAGGACTACGCGCGCCTGCTCGCCAACAGCAGCCGCTATTCCGAGGTGCTGGGCCAGACCAGCGCCAGCGGC
TTCGCCCGCAGCCTGCAGCAGGCCGGCTACGCCACCGACCCGATGTACGCCGACAAGCTCACCCGCATCATCGGCGGCAG
CACCCTGCGCGGCGCGCTTTCGGGCTGA

Upstream 100 bases:

>100_bases
GAGCTGGAGGTGATCTGAATGAGCACGTCTCGAACCGCGCGGCATCCGAGCTTTTCCCGGCAGGCGCAGGCCTGCCTTTG
CGCATTGGGGGAGGTCTGAG

Downstream 100 bases:

>100_bases
GCGGCAAAGGGCGGCGGCAAGCGGCCGATTTTGCCGGTCGCCGCCGCTGCCACGGTGCCGAAAGCGCCCCTTGTTGCCGC
TTCGCACTGGTTTTTCACCC

Product: peptidoglycan hydrolase

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MTAANTGFQLNALDPNSLGDLKRLARNNPDSPETLRAASKQFEALFLQMALKSMREATPGSTLFDNEQTKSYQSLLDQQL
ALNMAHSRNNGMSEALFRQLGGINGKGAATPAVAEGLPLPGAAGANPGFDISNAIRQSSSPAALMRQAQAIAAGAESRGG
ADGDSIGALISRLENAVRATRDKAAAGATAPADGNDGVADDVREFVNAVWPHAQSASRQTGIPAQFIVAQAALETGWGDK
VLRHADGRNSYNLFNIKAGAGWTGDTVTRKVTEYSGGSAYTEQARFRSYGSYAEAFQDYARLLANSSRYSEVLGQTSASG
FARSLQQAGYATDPMYADKLTRIIGGSTLRGALSG

Sequences:

>Translated_355_residues
MTAANTGFQLNALDPNSLGDLKRLARNNPDSPETLRAASKQFEALFLQMALKSMREATPGSTLFDNEQTKSYQSLLDQQL
ALNMAHSRNNGMSEALFRQLGGINGKGAATPAVAEGLPLPGAAGANPGFDISNAIRQSSSPAALMRQAQAIAAGAESRGG
ADGDSIGALISRLENAVRATRDKAAAGATAPADGNDGVADDVREFVNAVWPHAQSASRQTGIPAQFIVAQAALETGWGDK
VLRHADGRNSYNLFNIKAGAGWTGDTVTRKVTEYSGGSAYTEQARFRSYGSYAEAFQDYARLLANSSRYSEVLGQTSASG
FARSLQQAGYATDPMYADKLTRIIGGSTLRGALSG
>Mature_354_residues
TAANTGFQLNALDPNSLGDLKRLARNNPDSPETLRAASKQFEALFLQMALKSMREATPGSTLFDNEQTKSYQSLLDQQLA
LNMAHSRNNGMSEALFRQLGGINGKGAATPAVAEGLPLPGAAGANPGFDISNAIRQSSSPAALMRQAQAIAAGAESRGGA
DGDSIGALISRLENAVRATRDKAAAGATAPADGNDGVADDVREFVNAVWPHAQSASRQTGIPAQFIVAQAALETGWGDKV
LRHADGRNSYNLFNIKAGAGWTGDTVTRKVTEYSGGSAYTEQARFRSYGSYAEAFQDYARLLANSSRYSEVLGQTSASGF
ARSLQQAGYATDPMYADKLTRIIGGSTLRGALSG

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=333, Percent_Identity=35.7357357357357, Blast_Score=182, Evalue=2e-47,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR000423
- InterPro:   IPR019301
- InterPro:   IPR013338
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.-

Molecular weight: Translated: 37263; Mature: 37131

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAANTGFQLNALDPNSLGDLKRLARNNPDSPETLRAASKQFEALFLQMALKSMREATPG
CCCCCCCEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
STLFDNEQTKSYQSLLDQQLALNMAHSRNNGMSEALFRQLGGINGKGAATPAVAEGLPLP
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHCCCCCC
GAAGANPGFDISNAIRQSSSPAALMRQAQAIAAGAESRGGADGDSIGALISRLENAVRAT
CCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
RDKAAAGATAPADGNDGVADDVREFVNAVWPHAQSASRQTGIPAQFIVAQAALETGWGDK
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHH
VLRHADGRNSYNLFNIKAGAGWTGDTVTRKVTEYSGGSAYTEQARFRSYGSYAEAFQDYA
HHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHH
RLLANSSRYSEVLGQTSASGFARSLQQAGYATDPMYADKLTRIIGGSTLRGALSG
HHHHCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHCCCC
>Mature Secondary Structure 
TAANTGFQLNALDPNSLGDLKRLARNNPDSPETLRAASKQFEALFLQMALKSMREATPG
CCCCCCEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
STLFDNEQTKSYQSLLDQQLALNMAHSRNNGMSEALFRQLGGINGKGAATPAVAEGLPLP
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHCCCCCC
GAAGANPGFDISNAIRQSSSPAALMRQAQAIAAGAESRGGADGDSIGALISRLENAVRAT
CCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
RDKAAAGATAPADGNDGVADDVREFVNAVWPHAQSASRQTGIPAQFIVAQAALETGWGDK
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHH
VLRHADGRNSYNLFNIKAGAGWTGDTVTRKVTEYSGGSAYTEQARFRSYGSYAEAFQDYA
HHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHH
RLLANSSRYSEVLGQTSASGFARSLQQAGYATDPMYADKLTRIIGGSTLRGALSG
HHHHCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]