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The map label for this gene is fliN [H]

Identifier: 119899014

GI number: 119899014

Start: 3008899

End: 3009378

Strand: Direct

Name: fliN [H]

Synonym: azo2724

Alternate gene names: 119899014

Gene position: 3008899-3009378 (Clockwise)

Preceding gene: 119899013

Following gene: 119899015

Centisome position: 68.76

GC content: 66.25

Gene sequence:

>480_bases
ATGGCTGACAACGACGACGAAAACCAGATCTCCGAAGACGATTGGGCTGCCGCGATGCAGGAGCAGGCCAGCGCCGAGGC
GGGCATGGACGCAGAATCCGCGCGCGTCGCGGCCGACCTGGCCGCGGCGGCCGCCTTCGACGAATCGCCCTATCAGGCCA
AGCCGGCCAGCCAGATCTTCGAGGACTTCGGCTCGTCCGGCGCCAAGCCGGGGGCGCTCAACGACTTCGACATGATCCTG
GACATCCCGGTCCAGCTCACCGTCGAACTCGGCCGTACCAAGCTGTCCATCCGCAACCTGCTGCAGCTGGCCCACGGCTC
GGTGGTGGAACTCGACGGCCTCGCCGGCGAGCCGATGGACGTGCTGGTGAACGGCACTCTGATCGCGCAGGGCGAGGTCG
TGGTGGTGAACGACAAGTTCGGCATCCGCCTGACCGACATCATCACGCCCGCCGAACGCATGCGGAAGATACGACACTGA

Upstream 100 bases:

>100_bases
GAGTGCCGCTACGGCACCCAGAACGGCCAGTTCGCCTTCAAGGTGGATCGCTTCCTGGGCCCGGACGAGACCGACGCCCC
CAAGATTCCTGGAGCCACAA

Downstream 100 bases:

>100_bases
GCCTGACCGCCCGCCCCGCCACCCTCGCACGCCCCGCCCGCGCCTCCGCGCGGCGGGGCGTCCGCTCATGCGCGGCCGTG
CTCGCGCTGTACGGCGGCGC

Product: flagellar motor switch FliN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MADNDDENQISEDDWAAAMQEQASAEAGMDAESARVAADLAAAAAFDESPYQAKPASQIFEDFGSSGAKPGALNDFDMIL
DIPVQLTVELGRTKLSIRNLLQLAHGSVVELDGLAGEPMDVLVNGTLIAQGEVVVVNDKFGIRLTDIITPAERMRKIRH

Sequences:

>Translated_159_residues
MADNDDENQISEDDWAAAMQEQASAEAGMDAESARVAADLAAAAAFDESPYQAKPASQIFEDFGSSGAKPGALNDFDMIL
DIPVQLTVELGRTKLSIRNLLQLAHGSVVELDGLAGEPMDVLVNGTLIAQGEVVVVNDKFGIRLTDIITPAERMRKIRH
>Mature_158_residues
ADNDDENQISEDDWAAAMQEQASAEAGMDAESARVAADLAAAAAFDESPYQAKPASQIFEDFGSSGAKPGALNDFDMILD
IPVQLTVELGRTKLSIRNLLQLAHGSVVELDGLAGEPMDVLVNGTLIAQGEVVVVNDKFGIRLTDIITPAERMRKIRH

Specific function: FliN is one of three proteins (FliG, FliN, FliM) that form a switch complex that is proposed to be located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the motor

COG id: COG1886

COG function: function code NU; Flagellar motor switch/type III secretory pathway protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side. Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliN/MopA/SpaO family [H]

Homologues:

Organism=Escherichia coli, GI1788256, Length=153, Percent_Identity=50.3267973856209, Blast_Score=149, Evalue=1e-37,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012826
- InterPro:   IPR001172
- InterPro:   IPR001543 [H]

Pfam domain/function: PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 17011; Mature: 16880

Theoretical pI: Translated: 4.00; Mature: 4.00

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADNDDENQISEDDWAAAMQEQASAEAGMDAESARVAADLAAAAAFDESPYQAKPASQIF
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
EDFGSSGAKPGALNDFDMILDIPVQLTVELGRTKLSIRNLLQLAHGSVVELDGLAGEPMD
HHHCCCCCCCCCCCCCCEEEECCEEEEEECCCHHHHHHHHHHHHCCCEEEECCCCCCCCE
VLVNGTLIAQGEVVVVNDKFGIRLTDIITPAERMRKIRH
EEECCEEEECCCEEEEECCCCEEEHHHCCCHHHHHHHCC
>Mature Secondary Structure 
ADNDDENQISEDDWAAAMQEQASAEAGMDAESARVAADLAAAAAFDESPYQAKPASQIF
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
EDFGSSGAKPGALNDFDMILDIPVQLTVELGRTKLSIRNLLQLAHGSVVELDGLAGEPMD
HHHCCCCCCCCCCCCCCEEEECCEEEEEECCCHHHHHHHHHHHHCCCEEEECCCCCCCCE
VLVNGTLIAQGEVVVVNDKFGIRLTDIITPAERMRKIRH
EEECCEEEECCCEEEEECCCCEEEHHHCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2651416; 9097040; 9278503 [H]