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The map label for this gene is fliM [H]

Identifier: 119899013

GI number: 119899013

Start: 3007896

End: 3008906

Strand: Direct

Name: fliM [H]

Synonym: azo2723

Alternate gene names: 119899013

Gene position: 3007896-3008906 (Clockwise)

Preceding gene: 119899012

Following gene: 119899014

Centisome position: 68.74

GC content: 64.69

Gene sequence:

>1011_bases
ATGTCTTCGGATTTTCTCTCCCAGGAAGAAGTCGACGCCCTGCTGCGCGGCGTCAACGGGGAGAGCGATGACGGCGGCGG
CGCCGAGGTCGAGCAAGGGGGCGTACGCCCCTACAACCTCGCCACGCAGGAACGCATCGTCCGTGGGCGCATGCCCACGA
TGGAGCTGATCAACGAGCGGTTCGCGCGCTACCTCCGCATCGGCCTGTTCAACTACATGCACCGCAACGCCGAGGTGTCA
GTGGGGCCGATCAAGGTGCAGAAGTACGCCGAGTTCGTGCGCAACCTGGTGGTGCCGACCAACCTCAACCTGGTGCTCGG
CAAGCCATTGCGCGGCACAGGCCTGGTGGTGTTCGACCCCAACCTGGTGTTCCTGGTGGTGGACAACATGTTCGGCGGCG
ACGGCCGCTTCCACACCCGGGTGGAGGGGCGCGACTTCACGCCGACCGAGCAGCGCATCATCCAGGGCATGCTCAACGTG
GTGTTCACCGAGTACGCCAAGGCCTGGGCGCCAGTGTTCAAGCTCGAGCTGGAATACGTGCGCTCGGAGATGAACTCGCA
GTTCGCCAACATCGCCACGCCGTCCGAAATCGTGGTGTCCACCAGCTTTTCGCTGGAGCTGGGCGGTGCGCAGGCGGAGA
TGCACATCTGCTTCCCCTACTCGATGGTGGAGCCGATCCGCGACCTGCTCTACTCGACGATGCAGAGCGACCATCTCACC
CAGGACCGGCGCTGGATCACGCTGCTGTCGCGCCAGCTGCAGACGGCAGAGGTGGAACTGACCTGCAACCTCGGCACCGC
GCGCGTCACGCTGCGCGACATCGTCAACATGCGGGTGGGCGACGTGATTCCGCTGCAGGTTTCCGAACTGCTGCAGGCCA
CCGTGGATGGCGTGCCGGTGCTGGAGTGCCGCTACGGCACCCAGAACGGCCAGTTCGCCTTCAAGGTGGATCGCTTCCTG
GGCCCGGACGAGACCGACGCCCCCAAGATTCCTGGAGCCACAAATGGCTGA

Upstream 100 bases:

>100_bases
TCCCGCCCCCGCGTGAAGCCAACCGCCGCGCACCGCCGACCGGGCCGATCCAGGCGGTGCTGTTCAACTCCTTCATCATC
CAGTAAGCGGAGACGCCGCC

Downstream 100 bases:

>100_bases
CAACGACGACGAAAACCAGATCTCCGAAGACGATTGGGCTGCCGCGATGCAGGAGCAGGCCAGCGCCGAGGCGGGCATGG
ACGCAGAATCCGCGCGCGTC

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MSSDFLSQEEVDALLRGVNGESDDGGGAEVEQGGVRPYNLATQERIVRGRMPTMELINERFARYLRIGLFNYMHRNAEVS
VGPIKVQKYAEFVRNLVVPTNLNLVLGKPLRGTGLVVFDPNLVFLVVDNMFGGDGRFHTRVEGRDFTPTEQRIIQGMLNV
VFTEYAKAWAPVFKLELEYVRSEMNSQFANIATPSEIVVSTSFSLELGGAQAEMHICFPYSMVEPIRDLLYSTMQSDHLT
QDRRWITLLSRQLQTAEVELTCNLGTARVTLRDIVNMRVGDVIPLQVSELLQATVDGVPVLECRYGTQNGQFAFKVDRFL
GPDETDAPKIPGATNG

Sequences:

>Translated_336_residues
MSSDFLSQEEVDALLRGVNGESDDGGGAEVEQGGVRPYNLATQERIVRGRMPTMELINERFARYLRIGLFNYMHRNAEVS
VGPIKVQKYAEFVRNLVVPTNLNLVLGKPLRGTGLVVFDPNLVFLVVDNMFGGDGRFHTRVEGRDFTPTEQRIIQGMLNV
VFTEYAKAWAPVFKLELEYVRSEMNSQFANIATPSEIVVSTSFSLELGGAQAEMHICFPYSMVEPIRDLLYSTMQSDHLT
QDRRWITLLSRQLQTAEVELTCNLGTARVTLRDIVNMRVGDVIPLQVSELLQATVDGVPVLECRYGTQNGQFAFKVDRFL
GPDETDAPKIPGATNG
>Mature_335_residues
SSDFLSQEEVDALLRGVNGESDDGGGAEVEQGGVRPYNLATQERIVRGRMPTMELINERFARYLRIGLFNYMHRNAEVSV
GPIKVQKYAEFVRNLVVPTNLNLVLGKPLRGTGLVVFDPNLVFLVVDNMFGGDGRFHTRVEGRDFTPTEQRIIQGMLNVV
FTEYAKAWAPVFKLELEYVRSEMNSQFANIATPSEIVVSTSFSLELGGAQAEMHICFPYSMVEPIRDLLYSTMQSDHLTQ
DRRWITLLSRQLQTAEVELTCNLGTARVTLRDIVNMRVGDVIPLQVSELLQATVDGVPVLECRYGTQNGQFAFKVDRFLG
PDETDAPKIPGATNG

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=323, Percent_Identity=48.297213622291, Blast_Score=327, Evalue=8e-91,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 37558; Mature: 37427

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSDFLSQEEVDALLRGVNGESDDGGGAEVEQGGVRPYNLATQERIVRGRMPTMELINER
CCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHH
FARYLRIGLFNYMHRNAEVSVGPIKVQKYAEFVRNLVVPTNLNLVLGKPLRGTGLVVFDP
HHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCEEEEECC
NLVFLVVDNMFGGDGRFHTRVEGRDFTPTEQRIIQGMLNVVFTEYAKAWAPVFKLELEYV
CEEEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSEMNSQFANIATPSEIVVSTSFSLELGGAQAEMHICFPYSMVEPIRDLLYSTMQSDHLT
HHHHHHHHHCCCCCCCEEEEEEEEEEECCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCC
QDRRWITLLSRQLQTAEVELTCNLGTARVTLRDIVNMRVGDVIPLQVSELLQATVDGVPV
CHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEE
LECRYGTQNGQFAFKVDRFLGPDETDAPKIPGATNG
EEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSDFLSQEEVDALLRGVNGESDDGGGAEVEQGGVRPYNLATQERIVRGRMPTMELINER
CCCCCCHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHH
FARYLRIGLFNYMHRNAEVSVGPIKVQKYAEFVRNLVVPTNLNLVLGKPLRGTGLVVFDP
HHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCEEEEECC
NLVFLVVDNMFGGDGRFHTRVEGRDFTPTEQRIIQGMLNVVFTEYAKAWAPVFKLELEYV
CEEEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSEMNSQFANIATPSEIVVSTSFSLELGGAQAEMHICFPYSMVEPIRDLLYSTMQSDHLT
HHHHHHHHHCCCCCCCEEEEEEEEEEECCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCC
QDRRWITLLSRQLQTAEVELTCNLGTARVTLRDIVNMRVGDVIPLQVSELLQATVDGVPV
CHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEE
LECRYGTQNGQFAFKVDRFLGPDETDAPKIPGATNG
EEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 3519573; 9097040; 9278503; 2651416 [H]