Click here to switch to the map view.

The map label for this gene is fixL [H]

Identifier: 119898963

GI number: 119898963

Start: 2945927

End: 2947732

Strand: Reverse

Name: fixL [H]

Synonym: azo2672

Alternate gene names: 119898963

Gene position: 2947732-2945927 (Counterclockwise)

Preceding gene: 119898965

Following gene: 119898962

Centisome position: 67.36

GC content: 70.87

Gene sequence:

>1806_bases
GTGCCTGACCGTCTTGCGCGGTGGCTGTGGCTCACGCATTTTCTGCTGTGGCTCACGGCGGGGGCCTGGGCTGCGCCCGT
CCCCGGTGAGCACGCCGAATCCGTATCGCGCGTCCGCGTCGGCGTGCTTGCCGTGCTCGGCGCCGAGGCCGCGGTGGCGG
AATGGTCGCCGCTGATACGCCGGCTCGAAAATGCCTTGCCGGGCTGGCAGGTCAGTCTGGTGCAACTCGATCACGCCGGC
TTGCGGGCGGCGGTGCAGCGCGGCGACCTCGAATTCGTCATCACCAATCCGGGCCATTACGTCGAACTGGAGGCCGACCT
GGGCGTCAGCCGCATCCTGTCGCTGGACGCCGGAGGCGGCCGGTCGCCGCAGCGCGCCCTGGGCGCGGCGGTGATCGTGC
CGGCGGCGTCGGTCATCCGCGATCTTTCCGACCTGCGCGGCAAGCGGCTCGCCATCGTCGGGCGCGATGCGTTCGGCGGC
TACCAGATGGTGTGGGGTGCGCTCGACGGGCTGGGTCTCCAGCCGGAGCGGGATCTGGCGGAACTGTACGAGGTAGGTCT
GCCGATGGACCGGGTCGTCGCCGCGCTGGACGCCGGCAAAGCCGATGCCGGCGTGCTGCGCAGCTGCCTGCTGGAAAGCC
GGCCGGAGTGGGCCGACCGCTTTCGTGTGGTGGCGCCGCAGGCGGTTGCCGGTTTCCCGTGCGCGACGTCCACGCCGCTT
TATCCGGACTGGCCGCTGGCGGCCTTGCGCCACACCTCGCCGGATCTCGCGCGTGCGGTGGCGATCGCGCTGCTTGGCAT
GACGCGGGAGACGGACGGCCTCGCGTGGGCCGTTCCCGCCGATTACCAGTCGGTGCATGAACTCTTCCGCCGCCTGCAGA
TCGGCCCCTATGCCTACCTGCGCGAACCCACGTTGATGGCGCTGGCCGCGCGCTACTGGCCCTGGGTGGCCGCGTTGGCG
CTGCTGGTGGCGGCGTGGGCGCTGTACACGCTGCGGGTGGAGCACCTGGTGCATAGGCGTACCGCCGAGTTGCGCGCGGC
GCAGGAGCAGGCCGAGCATATGGCGCGGCTGTCGGTGCTCGGCGAGTTGTCGGGCACGCTTGCGCACGAACTCAATCAGC
CGCTGGCCACCATCACCAATTACGCCCACAGCCTCCGCCGCAGGGCGGACAACCAGCGCCTGACCGACGCCGCGGTGCGC
GAGGCCGCCGCCGAAATCGCGGAGCAGGCCGAACGCGCGGCGGGCATCCTTGGCCGCATCCGCCATTTCGCCCGCAAACG
GCGCAACAGCTGCGAGCGCCTGGTGCCCGCCGAAGTGGTGGGCGAGTCGGTCGAACTGTTCCGCGGCATGCTCGCGCGCG
CGCCGGCTGTCGCGGTGGAAGACGCGTTGCCGGCCGGCACCGTGATCGAGGCCGATCGCCTGCAAATCCAGCAGGTGTTG
CTCAATCTGCTGAAGAATGCCCACGACGCCAGCGAAGGCCTGCCGGACGCGCGCCGGCGGATCCATATCCGGCTGACGCC
CGCGGATGCAGGACTGTCGCCCGGGGTACTCATCGCGGTGCGCGACTTCGGCCGGGGCCTGGACGCCGCGGCGCATGGGC
GCCTGTTCGAGTCCTTCTTCACCACCAAGCCGGACGGACTGGGCCTCGGGCTGTCGATCTGCCGATCGATTGCCGAGGCG
CACGGGGGACGGCTCGATGCCGCGCCGGCCGCCGACGGCCCCGGCTGGGTATTCACCTTGAATCTGCCCGCCGTCCTTGC
GCCGGCGACGCCCACCGTGTTGCTCGACCATGACCCAAACCGATGA

Upstream 100 bases:

>100_bases
CATTGCTGGAACATCGAGCCCGAGAAGGGGCCCGACCCGGGGCGACCGATGGCATCATCCGCAGCCATGTCATACCGCGA
CCGCGCCCGCCGTGCCCCTC

Downstream 100 bases:

>100_bases
TTGCCTGATCCATGTCGTGGACGACGATGCGGCCTTCCGCCGCTCGCTGGTGTTCCTGTTCGAGTCGGTCGGCTGGCGGG
TGCGGCCCCACGCTTCCGCG

Product: putative sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 601; Mature: 600

Protein sequence:

>601_residues
MPDRLARWLWLTHFLLWLTAGAWAAPVPGEHAESVSRVRVGVLAVLGAEAAVAEWSPLIRRLENALPGWQVSLVQLDHAG
LRAAVQRGDLEFVITNPGHYVELEADLGVSRILSLDAGGGRSPQRALGAAVIVPAASVIRDLSDLRGKRLAIVGRDAFGG
YQMVWGALDGLGLQPERDLAELYEVGLPMDRVVAALDAGKADAGVLRSCLLESRPEWADRFRVVAPQAVAGFPCATSTPL
YPDWPLAALRHTSPDLARAVAIALLGMTRETDGLAWAVPADYQSVHELFRRLQIGPYAYLREPTLMALAARYWPWVAALA
LLVAAWALYTLRVEHLVHRRTAELRAAQEQAEHMARLSVLGELSGTLAHELNQPLATITNYAHSLRRRADNQRLTDAAVR
EAAAEIAEQAERAAGILGRIRHFARKRRNSCERLVPAEVVGESVELFRGMLARAPAVAVEDALPAGTVIEADRLQIQQVL
LNLLKNAHDASEGLPDARRRIHIRLTPADAGLSPGVLIAVRDFGRGLDAAAHGRLFESFFTTKPDGLGLGLSICRSIAEA
HGGRLDAAPAADGPGWVFTLNLPAVLAPATPTVLLDHDPNR

Sequences:

>Translated_601_residues
MPDRLARWLWLTHFLLWLTAGAWAAPVPGEHAESVSRVRVGVLAVLGAEAAVAEWSPLIRRLENALPGWQVSLVQLDHAG
LRAAVQRGDLEFVITNPGHYVELEADLGVSRILSLDAGGGRSPQRALGAAVIVPAASVIRDLSDLRGKRLAIVGRDAFGG
YQMVWGALDGLGLQPERDLAELYEVGLPMDRVVAALDAGKADAGVLRSCLLESRPEWADRFRVVAPQAVAGFPCATSTPL
YPDWPLAALRHTSPDLARAVAIALLGMTRETDGLAWAVPADYQSVHELFRRLQIGPYAYLREPTLMALAARYWPWVAALA
LLVAAWALYTLRVEHLVHRRTAELRAAQEQAEHMARLSVLGELSGTLAHELNQPLATITNYAHSLRRRADNQRLTDAAVR
EAAAEIAEQAERAAGILGRIRHFARKRRNSCERLVPAEVVGESVELFRGMLARAPAVAVEDALPAGTVIEADRLQIQQVL
LNLLKNAHDASEGLPDARRRIHIRLTPADAGLSPGVLIAVRDFGRGLDAAAHGRLFESFFTTKPDGLGLGLSICRSIAEA
HGGRLDAAPAADGPGWVFTLNLPAVLAPATPTVLLDHDPNR
>Mature_600_residues
PDRLARWLWLTHFLLWLTAGAWAAPVPGEHAESVSRVRVGVLAVLGAEAAVAEWSPLIRRLENALPGWQVSLVQLDHAGL
RAAVQRGDLEFVITNPGHYVELEADLGVSRILSLDAGGGRSPQRALGAAVIVPAASVIRDLSDLRGKRLAIVGRDAFGGY
QMVWGALDGLGLQPERDLAELYEVGLPMDRVVAALDAGKADAGVLRSCLLESRPEWADRFRVVAPQAVAGFPCATSTPLY
PDWPLAALRHTSPDLARAVAIALLGMTRETDGLAWAVPADYQSVHELFRRLQIGPYAYLREPTLMALAARYWPWVAALAL
LVAAWALYTLRVEHLVHRRTAELRAAQEQAEHMARLSVLGELSGTLAHELNQPLATITNYAHSLRRRADNQRLTDAAVRE
AAAEIAEQAERAAGILGRIRHFARKRRNSCERLVPAEVVGESVELFRGMLARAPAVAVEDALPAGTVIEADRLQIQQVLL
NLLKNAHDASEGLPDARRRIHIRLTPADAGLSPGVLIAVRDFGRGLDAAAHGRLFESFFTTKPDGLGLGLSICRSIAEAH
GGRLDAAPAADGPGWVFTLNLPAVLAPATPTVLLDHDPNR

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=248, Percent_Identity=31.8548387096774, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790436, Length=252, Percent_Identity=29.3650793650794, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786912, Length=269, Percent_Identity=26.3940520446097, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 64984; Mature: 64853

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDRLARWLWLTHFLLWLTAGAWAAPVPGEHAESVSRVRVGVLAVLGAEAAVAEWSPLIR
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
RLENALPGWQVSLVQLDHAGLRAAVQRGDLEFVITNPGHYVELEADLGVSRILSLDAGGG
HHHHCCCCCEEEEEEECHHHHHHHHHCCCEEEEEECCCCEEEEECCCCHHHHHEECCCCC
RSPQRALGAAVIVPAASVIRDLSDLRGKRLAIVGRDAFGGYQMVWGALDGLGLQPERDLA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCHHHH
ELYEVGLPMDRVVAALDAGKADAGVLRSCLLESRPEWADRFRVVAPQAVAGFPCATSTPL
HHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHEEECCHHHCCCCCCCCCCC
YPDWPLAALRHTSPDLARAVAIALLGMTRETDGLAWAVPADYQSVHELFRRLQIGPYAYL
CCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHCCCCHHHH
REPTLMALAARYWPWVAALALLVAAWALYTLRVEHLVHRRTAELRAAQEQAEHMARLSVL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GELSGTLAHELNQPLATITNYAHSLRRRADNQRLTDAAVREAAAEIAEQAERAAGILGRI
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHFARKRRNSCERLVPAEVVGESVELFRGMLARAPAVAVEDALPAGTVIEADRLQIQQVL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEECHHHHHHHHHH
LNLLKNAHDASEGLPDARRRIHIRLTPADAGLSPGVLIAVRDFGRGLDAAAHGRLFESFF
HHHHHCCCHHHCCCCCHHCEEEEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHH
TTKPDGLGLGLSICRSIAEAHGGRLDAAPAADGPGWVFTLNLPAVLAPATPTVLLDHDPN
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCEEECCCCCEEEECCCCC
R
C
>Mature Secondary Structure 
PDRLARWLWLTHFLLWLTAGAWAAPVPGEHAESVSRVRVGVLAVLGAEAAVAEWSPLIR
CHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
RLENALPGWQVSLVQLDHAGLRAAVQRGDLEFVITNPGHYVELEADLGVSRILSLDAGGG
HHHHCCCCCEEEEEEECHHHHHHHHHCCCEEEEEECCCCEEEEECCCCHHHHHEECCCCC
RSPQRALGAAVIVPAASVIRDLSDLRGKRLAIVGRDAFGGYQMVWGALDGLGLQPERDLA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCHHHH
ELYEVGLPMDRVVAALDAGKADAGVLRSCLLESRPEWADRFRVVAPQAVAGFPCATSTPL
HHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHEEECCHHHCCCCCCCCCCC
YPDWPLAALRHTSPDLARAVAIALLGMTRETDGLAWAVPADYQSVHELFRRLQIGPYAYL
CCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHCCCCHHHH
REPTLMALAARYWPWVAALALLVAAWALYTLRVEHLVHRRTAELRAAQEQAEHMARLSVL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GELSGTLAHELNQPLATITNYAHSLRRRADNQRLTDAAVREAAAEIAEQAERAAGILGRI
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHFARKRRNSCERLVPAEVVGESVELFRGMLARAPAVAVEDALPAGTVIEADRLQIQQVL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEECHHHHHHHHHH
LNLLKNAHDASEGLPDARRRIHIRLTPADAGLSPGVLIAVRDFGRGLDAAAHGRLFESFF
HHHHHCCCHHHCCCCCHHCEEEEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHH
TTKPDGLGLGLSICRSIAEAHGGRLDAAPAADGPGWVFTLNLPAVLAPATPTVLLDHDPN
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCEEECCCCCEEEECCCCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2842062; 11481432; 8432704 [H]