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The map label for this gene is fixJ1 [H]

Identifier: 119898962

GI number: 119898962

Start: 2945296

End: 2945943

Strand: Reverse

Name: fixJ1 [H]

Synonym: azo2671

Alternate gene names: 119898962

Gene position: 2945943-2945296 (Counterclockwise)

Preceding gene: 119898963

Following gene: 119898960

Centisome position: 67.32

GC content: 69.44

Gene sequence:

>648_bases
ATGACCCAAACCGATGATTGCCTGATCCATGTCGTGGACGACGATGCGGCCTTCCGCCGCTCGCTGGTGTTCCTGTTCGA
GTCGGTCGGCTGGCGGGTGCGGCCCCACGCTTCCGCGGAGGACTTTCTTGCCGAATGTGATACGGCGGCGGGCGGGGGCG
AGTGCGCGGACATTGCCTGCCTGGTGCTCGATATCCGGATGCCGACGATGAGCGGGCTGGAGTTGCAGCAGGCGATCCTG
GCACGTGGCTGGACGCTGCCCATCGTCTTCATCACCGGTCATGGCGACGTGGGGCTGGCGGTACAGGCGATGAAGTGCGG
CGCCAGCGACTTCCTCGAAAAGCCCTTCCACGACCAGGCGCTGCTCGACGCGGTGACGATGGCGATCCGCCGCGGCACCG
AGGCCCGGGCCGAACTCGCGCGTCGTGAGGACGCGGCCGCGGCGCTGGCCCGCCTCTCGCCGCGCGAGCGCGAGGTGGCG
CGGCTGGTAGCGCAGGGTCTGCCCAACAAGCGGGTGGCGCGCGCGCTCGGAATCAGCGAGAAGACGGTGCATGTGCACCG
TCAGCATGTGATGGAGAAGGCCGGCGTGGCCAGCGCGGCCGAACTCGCGCGCCTGATGCTGCGCGCCGATCCGGCCGCGC
TCGACTGA

Upstream 100 bases:

>100_bases
CGGCTCGATGCCGCGCCGGCCGCCGACGGCCCCGGCTGGGTATTCACCTTGAATCTGCCCGCCGTCCTTGCGCCGGCGAC
GCCCACCGTGTTGCTCGACC

Downstream 100 bases:

>100_bases
ACGGCCCGTCGTGCTTCAGTAGCCGGGGCGGCTGTCGGGCCGGCCGCTGCCGTTCATCATCCGGCCGTCGGGGCCCATGC
CGCCGCCGGGACCGCGCCCG

Product: two-component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MTQTDDCLIHVVDDDAAFRRSLVFLFESVGWRVRPHASAEDFLAECDTAAGGGECADIACLVLDIRMPTMSGLELQQAIL
ARGWTLPIVFITGHGDVGLAVQAMKCGASDFLEKPFHDQALLDAVTMAIRRGTEARAELARREDAAAALARLSPREREVA
RLVAQGLPNKRVARALGISEKTVHVHRQHVMEKAGVASAAELARLMLRADPAALD

Sequences:

>Translated_215_residues
MTQTDDCLIHVVDDDAAFRRSLVFLFESVGWRVRPHASAEDFLAECDTAAGGGECADIACLVLDIRMPTMSGLELQQAIL
ARGWTLPIVFITGHGDVGLAVQAMKCGASDFLEKPFHDQALLDAVTMAIRRGTEARAELARREDAAAALARLSPREREVA
RLVAQGLPNKRVARALGISEKTVHVHRQHVMEKAGVASAAELARLMLRADPAALD
>Mature_214_residues
TQTDDCLIHVVDDDAAFRRSLVFLFESVGWRVRPHASAEDFLAECDTAAGGGECADIACLVLDIRMPTMSGLELQQAILA
RGWTLPIVFITGHGDVGLAVQAMKCGASDFLEKPFHDQALLDAVTMAIRRGTEARAELARREDAAAALARLSPREREVAR
LVAQGLPNKRVARALGISEKTVHVHRQHVMEKAGVASAAELARLMLRADPAALD

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790102, Length=146, Percent_Identity=39.041095890411, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788521, Length=201, Percent_Identity=30.8457711442786, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1787473, Length=136, Percent_Identity=33.8235294117647, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1788222, Length=208, Percent_Identity=24.5192307692308, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23261; Mature: 23130

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTDDCLIHVVDDDAAFRRSLVFLFESVGWRVRPHASAEDFLAECDTAAGGGECADIAC
CCCCCCEEEEEECCCHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHCCCCCCCCHHHHHH
LVLDIRMPTMSGLELQQAILARGWTLPIVFITGHGDVGLAVQAMKCGASDFLEKPFHDQA
HEEEECCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHCCHHHHHHCCCCHHH
LLDAVTMAIRRGTEARAELARREDAAAALARLSPREREVARLVAQGLPNKRVARALGISE
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHCCCH
KTVHVHRQHVMEKAGVASAAELARLMLRADPAALD
HHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TQTDDCLIHVVDDDAAFRRSLVFLFESVGWRVRPHASAEDFLAECDTAAGGGECADIAC
CCCCCEEEEEECCCHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHCCCCCCCCHHHHHH
LVLDIRMPTMSGLELQQAILARGWTLPIVFITGHGDVGLAVQAMKCGASDFLEKPFHDQA
HEEEECCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHCCHHHHHHCCCCHHH
LLDAVTMAIRRGTEARAELARREDAAAALARLSPREREVARLVAQGLPNKRVARALGISE
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHCCCH
KTVHVHRQHVMEKAGVASAAELARLMLRADPAALD
HHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2046550 [H]