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The map label for this gene is pitA [H]

Identifier: 119898944

GI number: 119898944

Start: 2930124

End: 2931599

Strand: Direct

Name: pitA [H]

Synonym: azo2653

Alternate gene names: 119898944

Gene position: 2930124-2931599 (Clockwise)

Preceding gene: 119898943

Following gene: 119898946

Centisome position: 66.96

GC content: 66.12

Gene sequence:

>1476_bases
ATGATTCAACTGTTCAGCAGTTACGACCCCTGGCTGATCCTCAGCCTCGTCCTCGCGCTCGGCTTCGTCCTTGCCTTCGA
ATTCATCAACGGCTTTCACGACACCGCCAACGCCGTCGCCACGGTGATCTACACCCGCGCGATGCCGCCCTACCTGGCGG
TGATCGCCTCCGGCATCTTCAACTTCCTCGGCGTACTGCTCGGCGGTGTCGGCGTGGCCTACGCCATCGTGCACCTGCTG
CCGGTGGAGCTGCTGATCGACACCGACACCGGCCGCGGGCTGGCGATGGTGTTCTCGCTGCTCACCGCGGCGATCGCGTG
GAACCTAGGCACCTGGTACCTCGGCATCCCCGCGTCCAGCTCGCATACGCTGATCGGCTCCATCCTGGGCGTAGGCCTCG
CCAACGCGATGATCACCGACATCGGCGTCGCCGAAGGGGTGAACTGGCAGAAAGCGATCGACATCGGCCTGTCGCTGATC
GCGTCGCCGATGGCCGGCTTCGCGGTGGCGGGGCTGATCCTGCTGCTTTTGAAGTGGAAAAAGCCGCATTCGTCGATGCA
CCGCACGCCCGAATTCCGCATGGGCGAGGTCGGCAAGAAGCATCCGCCGTTCTGGGCGCGGCTGGTGCTGGTGCTGTCGG
CGATGGGGGTGAGCTTCGTGCATGGCTCCAACGACGGCCAGAAAGGCATCGGCCTGATCATGCTGGTGCTGATCGGCATC
GTGCCTGCACACTTCGTGCTCAACCTCAACAGCAGCGCCTACGACATCAGCCGCACGCGCGACGCCGCGATGCACCTGAG
CGAGTTCTATCAGCGCAATGCCGGCTCGCTGTCCGACTACCTCGCGCTGGGCAAATCCAACGGCGGCGACCTGCCGACCA
AGTTCCGCTGCGACCCGGCCCAGACCGAGATCACGATCGCCGCGCTGCAACGCAACCTCGACGGTGTGACGCGCTACGAG
GACATGCCGGAGCAGGCCCGCATCGACGCCCGCCGCTACCTGCTGTGCCTGGACGACACTGCGCGCAAGGTCTCCAAGCT
CGCCTACCTGCCCGCGGCCGAGAAGGCGGACCTCAACCGCCTGCGCAAGGACCTGACCTCCTCCACCGAATACGCGCCCT
TCTGGGTGGTGGTGGCGGTGGCGTTGGCCCTGGGCATCGGCACCATGATCGGCTGGAAGCGGGTGGTGCTGACCGTAGGC
GAGAAGATCGGCAAGGAAGGCATGACCTACGCGCAGGGCATGAGCGCGCAGGTCACCGCGGCGGCGGCCATCGGCATGGC
CAACGTGTTCTCGCTGCCGGTGTCGACCACCCACGTGCTGTCGTCCGGGGTGGCCGGCACCATGGTTGCCAACCGCAGCG
GCCTGCAGAGCCAGACGGTGAGCAACATCCTGCTCGCCTGGGTGCTGACGCTGCCGATCTCGATGGGCCTTGCCGCCGGC
CTGTACTGGCTGGCGTGGCGCACCTTCATCGCCTGA

Upstream 100 bases:

>100_bases
CGGCCGATCTGCGAAAATCCGCCGTCTTCATTTCCGGCAGTCCGGTCGCACCGGCGACGCGTGCCCTGCAGGCAGCAGCG
CCAACCGGCGACCCGCTCTT

Downstream 100 bases:

>100_bases
CCGCGTAATACGGGGCCGCGCAGCGCCGCGGCCCTAGCCCTTGCCGTCGCCGAACAGCGCCGCCACCCGCTTCTCGATCT
CGGCCGGGAGCAGGTCTTCC

Product: putative phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MIQLFSSYDPWLILSLVLALGFVLAFEFINGFHDTANAVATVIYTRAMPPYLAVIASGIFNFLGVLLGGVGVAYAIVHLL
PVELLIDTDTGRGLAMVFSLLTAAIAWNLGTWYLGIPASSSHTLIGSILGVGLANAMITDIGVAEGVNWQKAIDIGLSLI
ASPMAGFAVAGLILLLLKWKKPHSSMHRTPEFRMGEVGKKHPPFWARLVLVLSAMGVSFVHGSNDGQKGIGLIMLVLIGI
VPAHFVLNLNSSAYDISRTRDAAMHLSEFYQRNAGSLSDYLALGKSNGGDLPTKFRCDPAQTEITIAALQRNLDGVTRYE
DMPEQARIDARRYLLCLDDTARKVSKLAYLPAAEKADLNRLRKDLTSSTEYAPFWVVVAVALALGIGTMIGWKRVVLTVG
EKIGKEGMTYAQGMSAQVTAAAAIGMANVFSLPVSTTHVLSSGVAGTMVANRSGLQSQTVSNILLAWVLTLPISMGLAAG
LYWLAWRTFIA

Sequences:

>Translated_491_residues
MIQLFSSYDPWLILSLVLALGFVLAFEFINGFHDTANAVATVIYTRAMPPYLAVIASGIFNFLGVLLGGVGVAYAIVHLL
PVELLIDTDTGRGLAMVFSLLTAAIAWNLGTWYLGIPASSSHTLIGSILGVGLANAMITDIGVAEGVNWQKAIDIGLSLI
ASPMAGFAVAGLILLLLKWKKPHSSMHRTPEFRMGEVGKKHPPFWARLVLVLSAMGVSFVHGSNDGQKGIGLIMLVLIGI
VPAHFVLNLNSSAYDISRTRDAAMHLSEFYQRNAGSLSDYLALGKSNGGDLPTKFRCDPAQTEITIAALQRNLDGVTRYE
DMPEQARIDARRYLLCLDDTARKVSKLAYLPAAEKADLNRLRKDLTSSTEYAPFWVVVAVALALGIGTMIGWKRVVLTVG
EKIGKEGMTYAQGMSAQVTAAAAIGMANVFSLPVSTTHVLSSGVAGTMVANRSGLQSQTVSNILLAWVLTLPISMGLAAG
LYWLAWRTFIA
>Mature_491_residues
MIQLFSSYDPWLILSLVLALGFVLAFEFINGFHDTANAVATVIYTRAMPPYLAVIASGIFNFLGVLLGGVGVAYAIVHLL
PVELLIDTDTGRGLAMVFSLLTAAIAWNLGTWYLGIPASSSHTLIGSILGVGLANAMITDIGVAEGVNWQKAIDIGLSLI
ASPMAGFAVAGLILLLLKWKKPHSSMHRTPEFRMGEVGKKHPPFWARLVLVLSAMGVSFVHGSNDGQKGIGLIMLVLIGI
VPAHFVLNLNSSAYDISRTRDAAMHLSEFYQRNAGSLSDYLALGKSNGGDLPTKFRCDPAQTEITIAALQRNLDGVTRYE
DMPEQARIDARRYLLCLDDTARKVSKLAYLPAAEKADLNRLRKDLTSSTEYAPFWVVVAVALALGIGTMIGWKRVVLTVG
EKIGKEGMTYAQGMSAQVTAAAAIGMANVFSLPVSTTHVLSSGVAGTMVANRSGLQSQTVSNILLAWVLTLPISMGLAAG
LYWLAWRTFIA

Specific function: Low-affinity inorganic phosphate transport. Can also transport arsenate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789907, Length=474, Percent_Identity=54.4303797468354, Blast_Score=503, Evalue=1e-143,
Organism=Escherichia coli, GI1789360, Length=495, Percent_Identity=53.5353535353535, Blast_Score=497, Evalue=1e-142,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 52639; Mature: 52639

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQLFSSYDPWLILSLVLALGFVLAFEFINGFHDTANAVATVIYTRAMPPYLAVIASGIF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
NFLGVLLGGVGVAYAIVHLLPVELLIDTDTGRGLAMVFSLLTAAIAWNLGTWYLGIPASS
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCC
SHTLIGSILGVGLANAMITDIGVAEGVNWQKAIDIGLSLIASPMAGFAVAGLILLLLKWK
CHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KPHSSMHRTPEFRMGEVGKKHPPFWARLVLVLSAMGVSFVHGSNDGQKGIGLIMLVLIGI
CCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHH
VPAHFVLNLNSSAYDISRTRDAAMHLSEFYQRNAGSLSDYLALGKSNGGDLPTKFRCDPA
HHHHHEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCEECCCC
QTEITIAALQRNLDGVTRYEDMPEQARIDARRYLLCLDDTARKVSKLAYLPAAEKADLNR
HHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHEEEEECHHHHHHHHHHHCCCCCHHHHHH
LRKDLTSSTEYAPFWVVVAVALALGIGTMIGWKRVVLTVGEKIGKEGMTYAQGMSAQVTA
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHH
AAAIGMANVFSLPVSTTHVLSSGVAGTMVANRSGLQSQTVSNILLAWVLTLPISMGLAAG
HHHHHHHHHHHCCCHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LYWLAWRTFIA
HHHHHHHHHCC
>Mature Secondary Structure
MIQLFSSYDPWLILSLVLALGFVLAFEFINGFHDTANAVATVIYTRAMPPYLAVIASGIF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
NFLGVLLGGVGVAYAIVHLLPVELLIDTDTGRGLAMVFSLLTAAIAWNLGTWYLGIPASS
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCC
SHTLIGSILGVGLANAMITDIGVAEGVNWQKAIDIGLSLIASPMAGFAVAGLILLLLKWK
CHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KPHSSMHRTPEFRMGEVGKKHPPFWARLVLVLSAMGVSFVHGSNDGQKGIGLIMLVLIGI
CCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHH
VPAHFVLNLNSSAYDISRTRDAAMHLSEFYQRNAGSLSDYLALGKSNGGDLPTKFRCDPA
HHHHHEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCEECCCC
QTEITIAALQRNLDGVTRYEDMPEQARIDARRYLLCLDDTARKVSKLAYLPAAEKADLNR
HHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHEEEEECHHHHHHHHHHHCCCCCHHHHHH
LRKDLTSSTEYAPFWVVVAVALALGIGTMIGWKRVVLTVGEKIGKEGMTYAQGMSAQVTA
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHH
AAAIGMANVFSLPVSTTHVLSSGVAGTMVANRSGLQSQTVSNILLAWVLTLPISMGLAAG
HHHHHHHHHHHCCCHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LYWLAWRTFIA
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]