Click here to switch to the map view.

The map label for this gene is pilU1 [H]

Identifier: 119898843

GI number: 119898843

Start: 2822144

End: 2823451

Strand: Direct

Name: pilU1 [H]

Synonym: azo2552

Alternate gene names: 119898843

Gene position: 2822144-2823451 (Clockwise)

Preceding gene: 119898842

Following gene: 119898844

Centisome position: 64.49

GC content: 63.15

Gene sequence:

>1308_bases
ATGACCGCCTTCACCGAAGAACAGGCCCGTACCTACATGCACAAGCTGCTGGCTGCGATGAGCAACGCCGGCGGCTCGGA
CCTCTTCATCTCGCACGACTTCCCGCCCAGCATGAAGTCGCACGGCAGCATGCAGCCGATGACCAACCAGAAGCTCACCG
GCGAACTCACCCGCTCGCTGGCGTATTCGCTGATGAACGCGGCGCAGCGCGAGGAATTCGAGCGCGAGATGGAATGTAAC
TTCGCGATCAGCGTGCCGGGGCTGTCGCGCTTCCGCGTGAACGTGTTCGTGCAGCAGACCTATGTGGGCATGGTCATCCG
CACCATCGCGGCGGAAATCCCCAACTTCGAGAAGCTGTCCCTGCCCGAGGTGCTGAAGGAAGTCATCATGAACAAGCGCG
GGCTGGTGCTGGTGGTGGGCGGCACCGGCTCGGGCAAGTCGACCACGCTGGCGGCGATGATCGACTACCGCAACAGCCAT
TCGGCCGGGCACATCATCACCATCGAAGACCCGGTGGAGTACGTACACAAGTCGAAGAAGTCGCTGATCACCCACCGCGA
GGTCGGCGTCGACACCCACAGCTGGCACCACGCGCTGAAGAACACCCTGCGCCAGGCGCCCGACGTGATCCTGATCGGCG
AGATCCGCGACGCCGAAACCATGGAACACGCGATCGCCTTCGCCGAGACCGGCCACCTCTGCCTGGGCACGCTGCACGCC
AACAACGCCAACCAGACCATCGACCGCATCATCAACTTCTTCCCGGAAGAGCGGCGCAACCAGCTGCTGATGGACCTCTC
CTCCAACCTGCGCAGCATCATCTCGCAGCGCCTGGTGAAGACCGAGGACGGCAAGGGCCGGCGCGCCGCGATCGAGATCC
TGCTCAACACCTCGACCATCGCCGAGCGCATCTTCAAGGGCCAGTACAACGACATCAAGGCGATCATGGAGAAGTCGCGC
GAGCTGGGCATGCGCACCTTCGACTGGGCGCTGTTCGAGCTGTACAACGAAGGCCACATCTCCTACGAGGAAGCGATCCG
CAATGCGGACTCGGTAAACGAACTGCGCCTCGCGATCAAGCTCAAGAGCCAGCGCGGCGAACCGGGCACCGCCTCCGGCA
TGGAACTGAGCTTCGACCAGGCGCCGACCCCGGAAGAGCTGGAGGCGATGCGCGAAGAGGAGCAGAAGAACCAGGAAATC
CACCGCCAGGAATTCCAGCAGAAGGAACAGCAGAAGCCCCGCCTGGAACTGGAGCCGGAACCGGAGAAAACCCCGCCGGC
CGCCACCGCCGAACTCAAGCTGGAATGA

Upstream 100 bases:

>100_bases
CGGACCGCGCGGGGGGCGCTGCTACACTCGGCCGCAATCCCGATTGCCTAAGCCGGATGGATGCGCCCGCCGCGCCGCCG
CGCTCCACAAGGACGCCCCC

Downstream 100 bases:

>100_bases
CGGCCGCGCGGCGAGGCCGCGCTTTTGGCGTTGCCGTGGCGCAGCGCACGCGGCCTTGGCGGGCGTTCGCCCTACACTCG
GCGTGATTCGAACGGAATTA

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MTAFTEEQARTYMHKLLAAMSNAGGSDLFISHDFPPSMKSHGSMQPMTNQKLTGELTRSLAYSLMNAAQREEFEREMECN
FAISVPGLSRFRVNVFVQQTYVGMVIRTIAAEIPNFEKLSLPEVLKEVIMNKRGLVLVVGGTGSGKSTTLAAMIDYRNSH
SAGHIITIEDPVEYVHKSKKSLITHREVGVDTHSWHHALKNTLRQAPDVILIGEIRDAETMEHAIAFAETGHLCLGTLHA
NNANQTIDRIINFFPEERRNQLLMDLSSNLRSIISQRLVKTEDGKGRRAAIEILLNTSTIAERIFKGQYNDIKAIMEKSR
ELGMRTFDWALFELYNEGHISYEEAIRNADSVNELRLAIKLKSQRGEPGTASGMELSFDQAPTPEELEAMREEEQKNQEI
HRQEFQQKEQQKPRLELEPEPEKTPPAATAELKLE

Sequences:

>Translated_435_residues
MTAFTEEQARTYMHKLLAAMSNAGGSDLFISHDFPPSMKSHGSMQPMTNQKLTGELTRSLAYSLMNAAQREEFEREMECN
FAISVPGLSRFRVNVFVQQTYVGMVIRTIAAEIPNFEKLSLPEVLKEVIMNKRGLVLVVGGTGSGKSTTLAAMIDYRNSH
SAGHIITIEDPVEYVHKSKKSLITHREVGVDTHSWHHALKNTLRQAPDVILIGEIRDAETMEHAIAFAETGHLCLGTLHA
NNANQTIDRIINFFPEERRNQLLMDLSSNLRSIISQRLVKTEDGKGRRAAIEILLNTSTIAERIFKGQYNDIKAIMEKSR
ELGMRTFDWALFELYNEGHISYEEAIRNADSVNELRLAIKLKSQRGEPGTASGMELSFDQAPTPEELEAMREEEQKNQEI
HRQEFQQKEQQKPRLELEPEPEKTPPAATAELKLE
>Mature_434_residues
TAFTEEQARTYMHKLLAAMSNAGGSDLFISHDFPPSMKSHGSMQPMTNQKLTGELTRSLAYSLMNAAQREEFEREMECNF
AISVPGLSRFRVNVFVQQTYVGMVIRTIAAEIPNFEKLSLPEVLKEVIMNKRGLVLVVGGTGSGKSTTLAAMIDYRNSHS
AGHIITIEDPVEYVHKSKKSLITHREVGVDTHSWHHALKNTLRQAPDVILIGEIRDAETMEHAIAFAETGHLCLGTLHAN
NANQTIDRIINFFPEERRNQLLMDLSSNLRSIISQRLVKTEDGKGRRAAIEILLNTSTIAERIFKGQYNDIKAIMEKSRE
LGMRTFDWALFELYNEGHISYEEAIRNADSVNELRLAIKLKSQRGEPGTASGMELSFDQAPTPEELEAMREEEQKNQEIH
RQEFQQKEQQKPRLELEPEPEKTPPAATAELKLE

Specific function: May be involved in pilus retraction [H]

COG id: COG5008

COG function: function code NU; Tfp pilus assembly protein, ATPase PilU

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=317, Percent_Identity=35.6466876971609, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1789723, Length=156, Percent_Identity=42.3076923076923, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI1786296, Length=259, Percent_Identity=32.8185328185328, Blast_Score=101, Evalue=9e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 49174; Mature: 49042

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAFTEEQARTYMHKLLAAMSNAGGSDLFISHDFPPSMKSHGSMQPMTNQKLTGELTRSL
CCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHCCCCCCCCCCCHHHHHHHHHH
AYSLMNAAQREEFEREMECNFAISVPGLSRFRVNVFVQQTYVGMVIRTIAAEIPNFEKLS
HHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCC
LPEVLKEVIMNKRGLVLVVGGTGSGKSTTLAAMIDYRNSHSAGHIITIEDPVEYVHKSKK
HHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCCCEEEECCHHHHHHHHHH
SLITHREVGVDTHSWHHALKNTLRQAPDVILIGEIRDAETMEHAIAFAETGHLCLGTLHA
HHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEEC
NNANQTIDRIINFFPEERRNQLLMDLSSNLRSIISQRLVKTEDGKGRRAAIEILLNTSTI
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECHHHH
AERIFKGQYNDIKAIMEKSRELGMRTFDWALFELYNEGHISYEEAIRNADSVNELRLAIK
HHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHEEEEEE
LKSQRGEPGTASGMELSFDQAPTPEELEAMREEEQKNQEIHRQEFQQKEQQKPRLELEPE
EECCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PEKTPPAATAELKLE
CCCCCCCCCEEEEEC
>Mature Secondary Structure 
TAFTEEQARTYMHKLLAAMSNAGGSDLFISHDFPPSMKSHGSMQPMTNQKLTGELTRSL
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHCCCCCCCCCCCHHHHHHHHHH
AYSLMNAAQREEFEREMECNFAISVPGLSRFRVNVFVQQTYVGMVIRTIAAEIPNFEKLS
HHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCC
LPEVLKEVIMNKRGLVLVVGGTGSGKSTTLAAMIDYRNSHSAGHIITIEDPVEYVHKSKK
HHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCCCEEEECCHHHHHHHHHH
SLITHREVGVDTHSWHHALKNTLRQAPDVILIGEIRDAETMEHAIAFAETGHLCLGTLHA
HHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEEC
NNANQTIDRIINFFPEERRNQLLMDLSSNLRSIISQRLVKTEDGKGRRAAIEILLNTSTI
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECHHHH
AERIFKGQYNDIKAIMEKSRELGMRTFDWALFELYNEGHISYEEAIRNADSVNELRLAIK
HHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHEEEEEE
LKSQRGEPGTASGMELSFDQAPTPEELEAMREEEQKNQEIHRQEFQQKEQQKPRLELEPE
EECCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PEKTPPAATAELKLE
CCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]