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The map label for this gene is ligB1 [H]

Identifier: 119898813

GI number: 119898813

Start: 2788910

End: 2789752

Strand: Reverse

Name: ligB1 [H]

Synonym: azo2522

Alternate gene names: 119898813

Gene position: 2789752-2788910 (Counterclockwise)

Preceding gene: 119898814

Following gene: 119898812

Centisome position: 63.75

GC content: 65.6

Gene sequence:

>843_bases
ATGGCAAAGATCGTAGGCACCATCACCACTTCGCACGTCCCGGCCATCGGCCGCGCCCTCGCCGGCAAGCTGCAGGACGA
CCCGTACTGGAAGCCTTTCTTCGACGGCTTTCCGCCCGTGCATGCGTGGCTGGAGAAGGTGAAGCCGGACGTGGCGGTCG
TGCTGTACAACGACCACGGCCTCAACTTCTTCCTCGACAAGATGCCCACCTTCGCGGTGGGCGCGGCCCCGCAGTACCAC
AACGCCGATGAAGGCTGGGGCATTCCCACGCTGCCGCCCTATCAAGGCGATCCCGCGCTGTCGTGGCAGCTGATCGAAGA
CCTGGTGGCGCGCGAGTTCGATATCACCACCTGCCAGGAAATGCTGGTGGACCACGCGTTTTCGCTGCCGCTGAAGCTGC
TGTGGCCGGCGTCCGAAAGTTGCCCGGTGCGCACCGTGCCGGTCTGCATCAACACCGTGCAGTATCCGCTGCCCTCGGCC
CGGCGCTGCTACAAGCTGGGCAAGGCCATCGGCGAGGCGATTGCCGGCTGGGATGACGACCTGCGCGTGGTGATCATCGG
CACGGGCGGGCTGTCGCATCAGCTCGATGGAGAGCGCGCCGGCTTCATCAACAAGGACTTCGACCTCGCCTTCATGGAAA
GCCTGATCGCCGACCCCGAGTGGGCGACGCAGTTCTCGATGGAAGAACTGGTGGAGAAGACCGGCACGCAGGGCGTGGAA
CTGCTGATGTGGCTGGCCGCGCGGGCCGCGCTGCCGGGCGCGGTGCGCAAGGTGCACGCCAACTACCACATCCCCATCTC
CAACACCGCGGCGGGGCTGATGGTGATCGAAACGGTGGCCTGA

Upstream 100 bases:

>100_bases
CGGCATTCTGGGACTCGACGTGCAGGACCTGGGCGCTGCCCAGACCGGCATGACCAAGGAAGCGTTCAAGGCCATGCTCG
TGGCCGCGGGGAAATAAGAC

Downstream 100 bases:

>100_bases
GCGCCGCCGTCTGCTTCCCCTCGTGCCGCCCCGCTCGGCACTCCGGGGCCGGCTCCACCTGTGGGGCCGGCCTTTCTTAT
TGTGTTGTCGCGGAGAACGG

Product: protocatechuate 4,5-dioxygenase subunit beta

Products: NA

Alternate protein names: 4,5-PCD [H]

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MAKIVGTITTSHVPAIGRALAGKLQDDPYWKPFFDGFPPVHAWLEKVKPDVAVVLYNDHGLNFFLDKMPTFAVGAAPQYH
NADEGWGIPTLPPYQGDPALSWQLIEDLVAREFDITTCQEMLVDHAFSLPLKLLWPASESCPVRTVPVCINTVQYPLPSA
RRCYKLGKAIGEAIAGWDDDLRVVIIGTGGLSHQLDGERAGFINKDFDLAFMESLIADPEWATQFSMEELVEKTGTQGVE
LLMWLAARAALPGAVRKVHANYHIPISNTAAGLMVIETVA

Sequences:

>Translated_280_residues
MAKIVGTITTSHVPAIGRALAGKLQDDPYWKPFFDGFPPVHAWLEKVKPDVAVVLYNDHGLNFFLDKMPTFAVGAAPQYH
NADEGWGIPTLPPYQGDPALSWQLIEDLVAREFDITTCQEMLVDHAFSLPLKLLWPASESCPVRTVPVCINTVQYPLPSA
RRCYKLGKAIGEAIAGWDDDLRVVIIGTGGLSHQLDGERAGFINKDFDLAFMESLIADPEWATQFSMEELVEKTGTQGVE
LLMWLAARAALPGAVRKVHANYHIPISNTAAGLMVIETVA
>Mature_279_residues
AKIVGTITTSHVPAIGRALAGKLQDDPYWKPFFDGFPPVHAWLEKVKPDVAVVLYNDHGLNFFLDKMPTFAVGAAPQYHN
ADEGWGIPTLPPYQGDPALSWQLIEDLVAREFDITTCQEMLVDHAFSLPLKLLWPASESCPVRTVPVCINTVQYPLPSAR
RCYKLGKAIGEAIAGWDDDLRVVIIGTGGLSHQLDGERAGFINKDFDLAFMESLIADPEWATQFSMEELVEKTGTQGVEL
LMWLAARAALPGAVRKVHANYHIPISNTAAGLMVIETVA

Specific function: Responsible for the aromatic ring fission of protocatechuate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786544, Length=148, Percent_Identity=34.4594594594595, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004183 [H]

Pfam domain/function: PF02900 LigB [H]

EC number: =1.13.11.8 [H]

Molecular weight: Translated: 30737; Mature: 30605

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIVGTITTSHVPAIGRALAGKLQDDPYWKPFFDGFPPVHAWLEKVKPDVAVVLYNDHG
CCCEEEEHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCC
LNFFLDKMPTFAVGAAPQYHNADEGWGIPTLPPYQGDPALSWQLIEDLVAREFDITTCQE
CEEHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHH
MLVDHAFSLPLKLLWPASESCPVRTVPVCINTVQYPLPSARRCYKLGKAIGEAIAGWDDD
HHHHHHHCCCEEEEECCCCCCCCEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCC
LRVVIIGTGGLSHQLDGERAGFINKDFDLAFMESLIADPEWATQFSMEELVEKTGTQGVE
CEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHH
LLMWLAARAALPGAVRKVHANYHIPISNTAAGLMVIETVA
HHHHHHHHHHCCHHHHHHHCCEEECCCCCCCCEEEEEECC
>Mature Secondary Structure 
AKIVGTITTSHVPAIGRALAGKLQDDPYWKPFFDGFPPVHAWLEKVKPDVAVVLYNDHG
CCEEEEHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCC
LNFFLDKMPTFAVGAAPQYHNADEGWGIPTLPPYQGDPALSWQLIEDLVAREFDITTCQE
CEEHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHH
MLVDHAFSLPLKLLWPASESCPVRTVPVCINTVQYPLPSARRCYKLGKAIGEAIAGWDDD
HHHHHHHCCCEEEEECCCCCCCCEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCC
LRVVIIGTGGLSHQLDGERAGFINKDFDLAFMESLIADPEWATQFSMEELVEKTGTQGVE
CEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHH
LLMWLAARAALPGAVRKVHANYHIPISNTAAGLMVIETVA
HHHHHHHHHHCCHHHHHHHCCEEECCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2185230 [H]