| Definition | Azoarcus sp. BH72 chromosome, complete genome. |
|---|---|
| Accession | NC_008702 |
| Length | 4,376,040 |
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The map label for this gene is alsS [H]
Identifier: 119898765
GI number: 119898765
Start: 2732140
End: 2733846
Strand: Reverse
Name: alsS [H]
Synonym: azo2474
Alternate gene names: 119898765
Gene position: 2733846-2732140 (Counterclockwise)
Preceding gene: 119898766
Following gene: 119898764
Centisome position: 62.47
GC content: 68.25
Gene sequence:
>1707_bases ATGTACACAACGGGTACGGCATTTCTCGAGGCGCTCCACGAGCGTGGCGTCCGCCATCTGTTTGCCAACCTGGGCAGCGA CCATCCCGCCATCGTCGAGAGCATGGAGGAGGCGAAGGCCGCATCGCGCGAGGTGCCCGCGCTCATCACCTGTCCCAACG AGATGGTGGCGCTCAGTGCGGCCCAGGGGTACGCCCAGGTCACCGGTCAGCCGCAGGCCGTGCTGGTCCATGTGGAGTGC GGCACGCAGGCGCTCGCGGGTGCAGTGCATAACGTCGCCAAGGCGAGGGTGCCGGTGCTGATCTTTGCCGGCGCGTCGCC CTTCACCCAGGAAGGCGAGTTGCGCGGCAGCCGCAACGAGTTCATCCAGTGGATACAGGATGTGCATGATCAGCGCGGCA TCGTGCGCGGCTACACCCGCTACGACAACGAGATCCGCACCGGCCGCAACGTCAAGCAACTGGTCTATCGCGCGATGCAG ATTGCCTGCAGCGACCCGAAGGGGGCGGCCTACCTGACCGGGGCGCGCGAGGTCATGGAAGAGGAAGTGCCGCGGGTCGA GCTCGACGCCGGGCAGTGGCAGGCGATCGCCCCCTGCGCGCTGCCGGAGCAGGGGCTGCAGGCGCTGATCGCGGATCTCG CGGCCGCCCGTCATCCGGTGGTGGTGACGTCCTTCCTCGGCCGCAGCGCCGCCGCGGTGGCGGAGCTGGTGAAGCTGGTG GAGCGGCTCGCGATTCCGGTCATCGAGTCGGTGCCGTCCTATGTGAACATTCCGGCCGATCACCCGATGTACCTCGGCAA CCAATGGAATGCGCCGCGCCAGAACGAAACCCTGGCCGCAGCCGACCTGGTGCTCGTAATCGACAGCGACGTGCCCTGGA TTCCGCTCGTGAACCGTCCGAGCGAGGATGCGCGCGTGTATTACATCGACGTGGATCCGCTCAAGGAGCAGATGCCGTTG TGGTACATCCCGTCGCGTCAGGTATTCCGCGCGGATGCGGCCACGGCGCTGGCCCAGATCAATGCCGGTCTCGCCGGGGT GGCGCTCGATGCCGATCTGGTGGCGGCGCGGCGCGAGCGGGTCACGGCGCTGCATCGCCGGCGCGACGAACTGCTGAGGG CGCGCGAGGTGGCGGGCGAAACCATCACCCCGGCCTATCTTGCCGCCTGCGTGCGCGACTTCATCGATGACGACACCATC GTCTGCAACGAAGGCATCTCGAACTACGAAGCCATCATCGACCACCTCGGCATGCGCCGCCCCGGCAGCATGATCGCCAG CGGCGGCGGCTCGCTGGGCTGGAACGGCGGCGCGGCCATCGGCGTGAAGCTGGCCCACCCGGACAAGACGGTGGTCGCGA TGTGTGGTGACGGCAGCTACATGTTCACCGTCCCCTCCAGCGTGCACTGGATGGCGCGCCGCTATGCCACGCCCTTTGTC CAGATCGTGTTCAACAACGGCGGCTGGAAGTCCCCCAAGTTATCGACCCTGGCGGTGCATCCGGACGGCTACGCCAGCCG TTCGTCCGACCTGGGTGTGAGTTTCGACCCGCCCGCCGATTACGCCGGTATCGCCGCCGCGGCCGGCGGCGCCCTGGCGC TCAGGGTGTCGCGCCCCGAGGCGCTGCGGGACGCCCTGGCCCAGGCCTTCGCCGCCGTCCGTGGCGGCCGCTCCGCCGTG CTGGATGTTCAGCTCGACCCCCTGTAG
Upstream 100 bases:
>100_bases CCATCTATATCTCACGTATCGCCTGCTGAATTGCCGCTCCCCGCCTGCGCTCCTAAGGTGACGACCAACCGACACCGGTA TCACCCAGGAGAGGAGACGA
Downstream 100 bases:
>100_bases TCCCCGTCGGGTGCAGACGCCCGACCCAACAAATCAGACAGGAGACATCAACATGCATACGCTCATGGATCAGGCAGTCT GGCAGGACAAGCTCATGTGC
Product: acetolactate synthase catalytic subunit
Products: NA
Alternate protein names: BFD; BFDC [H]
Number of amino acids: Translated: 568; Mature: 568
Protein sequence:
>568_residues MYTTGTAFLEALHERGVRHLFANLGSDHPAIVESMEEAKAASREVPALITCPNEMVALSAAQGYAQVTGQPQAVLVHVEC GTQALAGAVHNVAKARVPVLIFAGASPFTQEGELRGSRNEFIQWIQDVHDQRGIVRGYTRYDNEIRTGRNVKQLVYRAMQ IACSDPKGAAYLTGAREVMEEEVPRVELDAGQWQAIAPCALPEQGLQALIADLAAARHPVVVTSFLGRSAAAVAELVKLV ERLAIPVIESVPSYVNIPADHPMYLGNQWNAPRQNETLAAADLVLVIDSDVPWIPLVNRPSEDARVYYIDVDPLKEQMPL WYIPSRQVFRADAATALAQINAGLAGVALDADLVAARRERVTALHRRRDELLRAREVAGETITPAYLAACVRDFIDDDTI VCNEGISNYEAIIDHLGMRRPGSMIASGGGSLGWNGGAAIGVKLAHPDKTVVAMCGDGSYMFTVPSSVHWMARRYATPFV QIVFNNGGWKSPKLSTLAVHPDGYASRSSDLGVSFDPPADYAGIAAAAGGALALRVSRPEALRDALAQAFAAVRGGRSAV LDVQLDPL
Sequences:
>Translated_568_residues MYTTGTAFLEALHERGVRHLFANLGSDHPAIVESMEEAKAASREVPALITCPNEMVALSAAQGYAQVTGQPQAVLVHVEC GTQALAGAVHNVAKARVPVLIFAGASPFTQEGELRGSRNEFIQWIQDVHDQRGIVRGYTRYDNEIRTGRNVKQLVYRAMQ IACSDPKGAAYLTGAREVMEEEVPRVELDAGQWQAIAPCALPEQGLQALIADLAAARHPVVVTSFLGRSAAAVAELVKLV ERLAIPVIESVPSYVNIPADHPMYLGNQWNAPRQNETLAAADLVLVIDSDVPWIPLVNRPSEDARVYYIDVDPLKEQMPL WYIPSRQVFRADAATALAQINAGLAGVALDADLVAARRERVTALHRRRDELLRAREVAGETITPAYLAACVRDFIDDDTI VCNEGISNYEAIIDHLGMRRPGSMIASGGGSLGWNGGAAIGVKLAHPDKTVVAMCGDGSYMFTVPSSVHWMARRYATPFV QIVFNNGGWKSPKLSTLAVHPDGYASRSSDLGVSFDPPADYAGIAAAAGGALALRVSRPEALRDALAQAFAAVRGGRSAV LDVQLDPL >Mature_568_residues MYTTGTAFLEALHERGVRHLFANLGSDHPAIVESMEEAKAASREVPALITCPNEMVALSAAQGYAQVTGQPQAVLVHVEC GTQALAGAVHNVAKARVPVLIFAGASPFTQEGELRGSRNEFIQWIQDVHDQRGIVRGYTRYDNEIRTGRNVKQLVYRAMQ IACSDPKGAAYLTGAREVMEEEVPRVELDAGQWQAIAPCALPEQGLQALIADLAAARHPVVVTSFLGRSAAAVAELVKLV ERLAIPVIESVPSYVNIPADHPMYLGNQWNAPRQNETLAAADLVLVIDSDVPWIPLVNRPSEDARVYYIDVDPLKEQMPL WYIPSRQVFRADAATALAQINAGLAGVALDADLVAARRERVTALHRRRDELLRAREVAGETITPAYLAACVRDFIDDDTI VCNEGISNYEAIIDHLGMRRPGSMIASGGGSLGWNGGAAIGVKLAHPDKTVVAMCGDGSYMFTVPSSVHWMARRYATPFV QIVFNNGGWKSPKLSTLAVHPDGYASRSSDLGVSFDPPADYAGIAAAAGGALALRVSRPEALRDALAQAFAAVRGGRSAV LDVQLDPL
Specific function: Valine and isoleucine biosynthesis; first step. [C]
COG id: COG0028
COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the TPP enzyme family [H]
Homologues:
Organism=Escherichia coli, GI1786717, Length=509, Percent_Identity=24.3614931237721, Blast_Score=73, Evalue=4e-14, Organism=Caenorhabditis elegans, GI17531299, Length=577, Percent_Identity=24.7833622183709, Blast_Score=74, Evalue=3e-13, Organism=Caenorhabditis elegans, GI17531301, Length=577, Percent_Identity=24.7833622183709, Blast_Score=73, Evalue=4e-13,
Paralogues:
None
Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012000 - InterPro: IPR012001 - InterPro: IPR000399 - InterPro: IPR011766 [H]
Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]
EC number: =4.1.1.7 [H]
Molecular weight: Translated: 61089; Mature: 61089
Theoretical pI: Translated: 5.77; Mature: 5.77
Prosite motif: PS00187 TPP_ENZYMES
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.2 %Cys (Translated Protein) 2.1 %Met (Translated Protein) 3.3 %Cys+Met (Translated Protein) 1.2 %Cys (Mature Protein) 2.1 %Met (Mature Protein) 3.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MYTTGTAFLEALHERGVRHLFANLGSDHPAIVESMEEAKAASREVPALITCPNEMVALSA CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEECCCHHEEEHH AQGYAQVTGQPQAVLVHVECGTQALAGAVHNVAKARVPVLIFAGASPFTQEGELRGSRNE HCCHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHH FIQWIQDVHDQRGIVRGYTRYDNEIRTGRNVKQLVYRAMQIACSDPKGAAYLTGAREVME HHHHHHHHHHCCCCEECHHHCCCHHHCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHH EEVPRVELDAGQWQAIAPCALPEQGLQALIADLAAARHPVVVTSFLGRSAAAVAELVKLV HHCCEEEECCCCCCEECCCCCCHHHHHHHHHHHHHCCCCEEEHHHHCCHHHHHHHHHHHH ERLAIPVIESVPSYVNIPADHPMYLGNQWNAPRQNETLAAADLVLVIDSDVPWIPLVNRP HHHHHHHHHHCCCCEECCCCCCEEECCCCCCCCCCCCEEEEEEEEEEECCCCEEEECCCC SEDARVYYIDVDPLKEQMPLWYIPSRQVFRADAATALAQINAGLAGVALDADLVAARRER CCCCEEEEEECCCHHHCCCEEECCCCHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHH VTALHRRRDELLRAREVAGETITPAYLAACVRDFIDDDTIVCNEGISNYEAIIDHLGMRR HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHCCCC PGSMIASGGGSLGWNGGAAIGVKLAHPDKTVVAMCGDGSYMFTVPSSVHWMARRYATPFV CCCCEECCCCCCCCCCCCEEEEEEECCCCEEEEEECCCCEEEECCCHHHHHHHHHCCCEE QIVFNNGGWKSPKLSTLAVHPDGYASRSSDLGVSFDPPADYAGIAAAAGGALALRVSRPE EEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCEEEEEECCCH ALRDALAQAFAAVRGGRSAVLDVQLDPL HHHHHHHHHHHHHHCCCCEEEEEEECCC >Mature Secondary Structure MYTTGTAFLEALHERGVRHLFANLGSDHPAIVESMEEAKAASREVPALITCPNEMVALSA CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEECCCHHEEEHH AQGYAQVTGQPQAVLVHVECGTQALAGAVHNVAKARVPVLIFAGASPFTQEGELRGSRNE HCCHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHH FIQWIQDVHDQRGIVRGYTRYDNEIRTGRNVKQLVYRAMQIACSDPKGAAYLTGAREVME HHHHHHHHHHCCCCEECHHHCCCHHHCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHH EEVPRVELDAGQWQAIAPCALPEQGLQALIADLAAARHPVVVTSFLGRSAAAVAELVKLV HHCCEEEECCCCCCEECCCCCCHHHHHHHHHHHHHCCCCEEEHHHHCCHHHHHHHHHHHH ERLAIPVIESVPSYVNIPADHPMYLGNQWNAPRQNETLAAADLVLVIDSDVPWIPLVNRP HHHHHHHHHHCCCCEECCCCCCEEECCCCCCCCCCCCEEEEEEEEEEECCCCEEEECCCC SEDARVYYIDVDPLKEQMPLWYIPSRQVFRADAATALAQINAGLAGVALDADLVAARRER CCCCEEEEEECCCHHHCCCEEECCCCHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHH VTALHRRRDELLRAREVAGETITPAYLAACVRDFIDDDTIVCNEGISNYEAIIDHLGMRR HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHCCCC PGSMIASGGGSLGWNGGAAIGVKLAHPDKTVVAMCGDGSYMFTVPSSVHWMARRYATPFV CCCCEECCCCCCCCCCCCEEEEEEECCCCEEEEEECCCCEEEECCCHHHHHHHHHCCCEE QIVFNNGGWKSPKLSTLAVHPDGYASRSSDLGVSFDPPADYAGIAAAAGGALALRVSRPE EEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCEEEEEECCCH ALRDALAQAFAAVRGGRSAVLDVQLDPL HHHHHHHHHHHHHHCCCCEEEEEEECCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 2271624; 7663351; 9665697 [H]