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The map label for this gene is rpfG [H]

Identifier: 119898740

GI number: 119898740

Start: 2702082

End: 2703143

Strand: Reverse

Name: rpfG [H]

Synonym: azo2449

Alternate gene names: 119898740

Gene position: 2703143-2702082 (Counterclockwise)

Preceding gene: 119898741

Following gene: 119898738

Centisome position: 61.77

GC content: 68.83

Gene sequence:

>1062_bases
ATGCCCGCAACGCTGACATCCACTGCCCGCATTCTCGCGATCGACGACGAGCCGGCCAATCTCAAACTGCTGGAGAAGAT
CCTCGGCAGCCAGGGCTACGGCACCTGCGCGCTGGTGCAGGACCCGCGCGAGGCGGTGGCCCGCTACCTGGAGGTCAAGC
CGGACCTGATCCTGCTCGACATCAACATGCCGCACCTGGACGGCTTTGCGGTGATGACGCAGTTGCAGGCGCTGCAGGAT
CCGCTGCTGCCGCCCATCGTCATCCTCACCGCGCAGGCGGGGCGCGAATTCCTGCTGAAGGCGCTCGACCACGGCGCGCG
CGACTTCGTCGCCAAGCCTTTCGACCGCGTCGAGCTGCTGATGCGGGTGCGCAACCTGCTGGAAGCCCAGCTCGCCGCCC
GCCTGCTGCACGACCGCCAGGCGGTGCTGGAGGACATGGTGCGGGTGCGTACCGCCGAGCTGCAGGAAACCCGCCTGAGC
GTGGTGCGGCGGCTGGGCCGCGCGGCCGAATACCGCGACAACGAAACCGGCGTGCACATCGTGCGCATGAGCCGCTGCTG
CGCGCTGCTGGCCGCCCGCCTGGGCTGGAGCGCCGACGCCTGCGAACTGATCCTGCACGCCAGCCCGATGCACGACATCG
GCAAGATCGGCATCGCCGATGCCATCCTGCTCAAGCCGGGGCAGCTCGATCCGGACGAGTGGGAAATCATGAAGAGCCAC
ACCATGATCGGCGCCGAACTGCTCGCGGGCGATGACTCCGAACTGTTCCGGATGGCGCGCGAGATTGCGCTGACCCACCA
CGAAAAATGGGACGGCAGCGGCTATCCCCACGGCCTCGCGGGCGAGGCGATACCGCTCGCGGGCCGCATCGTGGCGGTGG
CGGACGTGTTCGATGCGCTGACCTCGCGCCGTCCGTACAAAGGACCGTGGACCACGGAGGCGGCGGTGGACTTCATCCGC
GAGCGCGCCGGCAGCGCTTTCGACCCCGCGGTGGTCGAGCACTTCCTCGCGGTGCTGCCCGAGGTCGCCGCCATCCGCAG
CGCCAGCCCCGATCCCGATTAG

Upstream 100 bases:

>100_bases
GTCAAACCGCTGGTCATCGACAGCTTCTTCGCGGCGATCGACCGCTGCCTGAACCGCGGCGTCGGCCAAGCCGGCGCAGC
GGCACAACCTTGAGGCATCC

Downstream 100 bases:

>100_bases
CGCCTGCCCGGTAAACGAGAAGGGGCGCAGCCCTGAAGCCGCGCCCCGTGTTGCTGCCCTGCCAGCCGTCGATTACTCGA
AGGTCGGATCGAACGGCAGG

Product: putative two component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MPATLTSTARILAIDDEPANLKLLEKILGSQGYGTCALVQDPREAVARYLEVKPDLILLDINMPHLDGFAVMTQLQALQD
PLLPPIVILTAQAGREFLLKALDHGARDFVAKPFDRVELLMRVRNLLEAQLAARLLHDRQAVLEDMVRVRTAELQETRLS
VVRRLGRAAEYRDNETGVHIVRMSRCCALLAARLGWSADACELILHASPMHDIGKIGIADAILLKPGQLDPDEWEIMKSH
TMIGAELLAGDDSELFRMAREIALTHHEKWDGSGYPHGLAGEAIPLAGRIVAVADVFDALTSRRPYKGPWTTEAAVDFIR
ERAGSAFDPAVVEHFLAVLPEVAAIRSASPDPD

Sequences:

>Translated_353_residues
MPATLTSTARILAIDDEPANLKLLEKILGSQGYGTCALVQDPREAVARYLEVKPDLILLDINMPHLDGFAVMTQLQALQD
PLLPPIVILTAQAGREFLLKALDHGARDFVAKPFDRVELLMRVRNLLEAQLAARLLHDRQAVLEDMVRVRTAELQETRLS
VVRRLGRAAEYRDNETGVHIVRMSRCCALLAARLGWSADACELILHASPMHDIGKIGIADAILLKPGQLDPDEWEIMKSH
TMIGAELLAGDDSELFRMAREIALTHHEKWDGSGYPHGLAGEAIPLAGRIVAVADVFDALTSRRPYKGPWTTEAAVDFIR
ERAGSAFDPAVVEHFLAVLPEVAAIRSASPDPD
>Mature_352_residues
PATLTSTARILAIDDEPANLKLLEKILGSQGYGTCALVQDPREAVARYLEVKPDLILLDINMPHLDGFAVMTQLQALQDP
LLPPIVILTAQAGREFLLKALDHGARDFVAKPFDRVELLMRVRNLLEAQLAARLLHDRQAVLEDMVRVRTAELQETRLSV
VRRLGRAAEYRDNETGVHIVRMSRCCALLAARLGWSADACELILHASPMHDIGKIGIADAILLKPGQLDPDEWEIMKSHT
MIGAELLAGDDSELFRMAREIALTHHEKWDGSGYPHGLAGEAIPLAGRIVAVADVFDALTSRRPYKGPWTTEAAVDFIRE
RAGSAFDPAVVEHFLAVLPEVAAIRSASPDPD

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=140, Percent_Identity=33.5714285714286, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI87082012, Length=117, Percent_Identity=37.6068376068376, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1790437, Length=105, Percent_Identity=34.2857142857143, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790299, Length=105, Percent_Identity=32.3809523809524, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 38810; Mature: 38679

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPATLTSTARILAIDDEPANLKLLEKILGSQGYGTCALVQDPREAVARYLEVKPDLILLD
CCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCEEEEE
INMPHLDGFAVMTQLQALQDPLLPPIVILTAQAGREFLLKALDHGARDFVAKPFDRVELL
CCCCCCCHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHCCHHHHHHCCHHHHHHH
MRVRNLLEAQLAARLLHDRQAVLEDMVRVRTAELQETRLSVVRRLGRAAEYRDNETGVHI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEH
VRMSRCCALLAARLGWSADACELILHASPMHDIGKIGIADAILLKPGQLDPDEWEIMKSH
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHCHHHHEEECCCCCCCHHHHHHHHH
TMIGAELLAGDDSELFRMAREIALTHHEKWDGSGYPHGLAGEAIPLAGRIVAVADVFDAL
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
TSRRPYKGPWTTEAAVDFIRERAGSAFDPAVVEHFLAVLPEVAAIRSASPDPD
HCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PATLTSTARILAIDDEPANLKLLEKILGSQGYGTCALVQDPREAVARYLEVKPDLILLD
CCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCEEEEE
INMPHLDGFAVMTQLQALQDPLLPPIVILTAQAGREFLLKALDHGARDFVAKPFDRVELL
CCCCCCCHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHCCHHHHHHCCHHHHHHH
MRVRNLLEAQLAARLLHDRQAVLEDMVRVRTAELQETRLSVVRRLGRAAEYRDNETGVHI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEH
VRMSRCCALLAARLGWSADACELILHASPMHDIGKIGIADAILLKPGQLDPDEWEIMKSH
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHCHHHHEEECCCCCCCHHHHHHHHH
TMIGAELLAGDDSELFRMAREIALTHHEKWDGSGYPHGLAGEAIPLAGRIVAVADVFDAL
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
TSRRPYKGPWTTEAAVDFIRERAGSAFDPAVVEHFLAVLPEVAAIRSASPDPD
HCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]