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The map label for this gene is rpfC [H]

Identifier: 119898653

GI number: 119898653

Start: 2601814

End: 2603754

Strand: Reverse

Name: rpfC [H]

Synonym: azo2362

Alternate gene names: 119898653

Gene position: 2603754-2601814 (Counterclockwise)

Preceding gene: 119898654

Following gene: 119898652

Centisome position: 59.5

GC content: 61.93

Gene sequence:

>1941_bases
ATGAACAGCTCGCAAACCGATTCGCTCCTGAAGGCGGAACTCCAGCAAGGGCGGGCCCTGCTCGCCAGTGTTGCGGCCAC
CACGGATGTGATGTTGGTGTACCTCGATCCCGGCTTCAATTTCGTGTGGGTGAATGAGGCTTACGCGCGCACTTGCCGGA
TGTCGCCCGGCGATATGGTCGGCAAGAACCATTTCGCGCTTTACCCGCATGCTGAAAATGAGGCGATCTTCCGGCAGGTC
AGGGATAGCGGAAATCCGGTTTTCTACAAGGACAAGCCTTTCGAGTTTGCCGACCAGCAGGACCGCGGGACCACATACTG
GGACTGGAGCCTTGCCGCCGACAAGGACAGCGACGGACGCGTCGTCGGCCTCGTATTCAGTCTGCGTGAAACCACCGGCT
TTGTACGCGCACGGCAGACCGTGCGGGAAACCGAAAACCTGCTGGCGACCTTCATCGAAAGCGTGCCCGCGGGCATTGCG
ATGTTCGATCGGGAGATGCGTTACCTCGCCGTCAGCCGCCGTTTCCTGCTCGACCATCGAATTATCGATGAGGACGTCAT
CGGCCGCTGTCATTACGAGGTGTTTCCCGATCTGCCCGAACGCTGGAAAGCAATTCATCGCCGTTGCCTGGCAGGTGAAA
CGCTTCGCTGCGACGAGGATCAATTCGTCCGCCGCAACGGTGAAATCAATTGGGAGCGCTGGGAGGTTCGCCCGTGGTTT
ACCGGCGCGGGTACGATTGGCGGCATCATCCTGTTCTCCGAAAACGTCACCGACCGCAAGCGCCAGGAGAACGGCCTGAA
GGCCGCGATGGAGGAGGCGGAGCGGGCAAATAATGCCAAGTCGCGCTTTCTCGCCGCGGCAAGCCACGATTTGCGGCAGC
CCTTGTCGGCCTTGTCGCTTTATGTGGGCACCCTGGAGAGAAAACTTCCGGCGTCCGAACGCGCGCTCGCTTCGGGCATC
GGGGATTGCGTCACCCACCTGAGTGAATTGCTGTCGAATCTGCTTGATCTCTCCAAGCTCGAAGCGGGCGTCATCAAGCC
GAGCGTCTGCGATTTCGAGGTGGACGCCGTGCTGTTGCGCATTGCGTTGTCTTATGAGCCGGAGGCGCGGGAGAAAGGCC
TGGGTTTGCGCTACACCGGTTCCGCTTGGGTCGCCCGCACCGACCCCGTGCTTTTTCAACGGGTCGTCACCAATCTGGTC
GCCAATGCGGTGCGCTACACGGAGCACGGCGGCATCCTGATCGGCGTCCGGCGACGAAGCGGAAAGCACTGGGTCGAGGT
GTGGGATACCGGCATCGGAATTCCCGCGGACAAGACGACCGAGATATTCGAGGAGTTCAGGCAGCTGGGAAATGCCGAGC
GCAACAGGACCAAGGGCTCGGGGATCGGCCTGACCATTGCGGCAAAGACGGCGAAATTGCTCGGGCTGCAGTTGCAAGTC
CGTTCCCGGCCAGGTCGCGGTTCGGTTTTCGCGCTCGAATTGCCCTTGGGCGAGCGGGTGGACGCCCACCCCGTCGAACC
GCCCCCGATTCACCAGAAGCGGCGTATTGCAATCGTCGAGGACAATACCGAGGTGGCCGCGGCGCTGGCTTATGCGCTAA
CCGAACTGGGTCATCAGGTGATATCCGCAGCCTCGCGGTACGAGGTCGTGACCCGGCTCAACGGCACTGCGCCGGAAATC
ATCCTTGCGGACTATCGGCTGGGCGGAGAAGAAGACGGCGTCGGTGTCATTCGCGCGGTGCGATCTGCGTTCGGAAACGA
CGTGCCCGCGCTGATCATTACCGGAGACACGGATCCGGTGGTCGTTCGCCGCATGGCGGATCACGGCATCAGCGTTCTGC
ACAAGCCGCTGGACCTGGACGCCCTGCAGGACCGGATTGCGGCGCTGATTGAAGCGCGCGCTGTGTCTTCGCCCCACCGG
ACGGCGGAGGGTGCCGGATAG

Upstream 100 bases:

>100_bases
GCGACAGTTTCGTTGCATTCCTGGTACGCCCAATCTGCATGATTGCCAGCGAGCATAAGCGGCGCGTAGCATCGGTCATC
CAGAATGGAAGACCGTGGCG

Downstream 100 bases:

>100_bases
ACCGGGACCCGGTGGCGGTCGGGAAAAACGGCCGCTCAAAACACAGCAGGCGGGGCGTTTGAAGCCGCCTTCGATGTCGC
CTCCGTCCTATCACCGCATC

Product: putative sensor kinase/response regulator hybrid protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 646; Mature: 646

Protein sequence:

>646_residues
MNSSQTDSLLKAELQQGRALLASVAATTDVMLVYLDPGFNFVWVNEAYARTCRMSPGDMVGKNHFALYPHAENEAIFRQV
RDSGNPVFYKDKPFEFADQQDRGTTYWDWSLAADKDSDGRVVGLVFSLRETTGFVRARQTVRETENLLATFIESVPAGIA
MFDREMRYLAVSRRFLLDHRIIDEDVIGRCHYEVFPDLPERWKAIHRRCLAGETLRCDEDQFVRRNGEINWERWEVRPWF
TGAGTIGGIILFSENVTDRKRQENGLKAAMEEAERANNAKSRFLAAASHDLRQPLSALSLYVGTLERKLPASERALASGI
GDCVTHLSELLSNLLDLSKLEAGVIKPSVCDFEVDAVLLRIALSYEPEAREKGLGLRYTGSAWVARTDPVLFQRVVTNLV
ANAVRYTEHGGILIGVRRRSGKHWVEVWDTGIGIPADKTTEIFEEFRQLGNAERNRTKGSGIGLTIAAKTAKLLGLQLQV
RSRPGRGSVFALELPLGERVDAHPVEPPPIHQKRRIAIVEDNTEVAAALAYALTELGHQVISAASRYEVVTRLNGTAPEI
ILADYRLGGEEDGVGVIRAVRSAFGNDVPALIITGDTDPVVVRRMADHGISVLHKPLDLDALQDRIAALIEARAVSSPHR
TAEGAG

Sequences:

>Translated_646_residues
MNSSQTDSLLKAELQQGRALLASVAATTDVMLVYLDPGFNFVWVNEAYARTCRMSPGDMVGKNHFALYPHAENEAIFRQV
RDSGNPVFYKDKPFEFADQQDRGTTYWDWSLAADKDSDGRVVGLVFSLRETTGFVRARQTVRETENLLATFIESVPAGIA
MFDREMRYLAVSRRFLLDHRIIDEDVIGRCHYEVFPDLPERWKAIHRRCLAGETLRCDEDQFVRRNGEINWERWEVRPWF
TGAGTIGGIILFSENVTDRKRQENGLKAAMEEAERANNAKSRFLAAASHDLRQPLSALSLYVGTLERKLPASERALASGI
GDCVTHLSELLSNLLDLSKLEAGVIKPSVCDFEVDAVLLRIALSYEPEAREKGLGLRYTGSAWVARTDPVLFQRVVTNLV
ANAVRYTEHGGILIGVRRRSGKHWVEVWDTGIGIPADKTTEIFEEFRQLGNAERNRTKGSGIGLTIAAKTAKLLGLQLQV
RSRPGRGSVFALELPLGERVDAHPVEPPPIHQKRRIAIVEDNTEVAAALAYALTELGHQVISAASRYEVVTRLNGTAPEI
ILADYRLGGEEDGVGVIRAVRSAFGNDVPALIITGDTDPVVVRRMADHGISVLHKPLDLDALQDRIAALIEARAVSSPHR
TAEGAG
>Mature_646_residues
MNSSQTDSLLKAELQQGRALLASVAATTDVMLVYLDPGFNFVWVNEAYARTCRMSPGDMVGKNHFALYPHAENEAIFRQV
RDSGNPVFYKDKPFEFADQQDRGTTYWDWSLAADKDSDGRVVGLVFSLRETTGFVRARQTVRETENLLATFIESVPAGIA
MFDREMRYLAVSRRFLLDHRIIDEDVIGRCHYEVFPDLPERWKAIHRRCLAGETLRCDEDQFVRRNGEINWERWEVRPWF
TGAGTIGGIILFSENVTDRKRQENGLKAAMEEAERANNAKSRFLAAASHDLRQPLSALSLYVGTLERKLPASERALASGI
GDCVTHLSELLSNLLDLSKLEAGVIKPSVCDFEVDAVLLRIALSYEPEAREKGLGLRYTGSAWVARTDPVLFQRVVTNLV
ANAVRYTEHGGILIGVRRRSGKHWVEVWDTGIGIPADKTTEIFEEFRQLGNAERNRTKGSGIGLTIAAKTAKLLGLQLQV
RSRPGRGSVFALELPLGERVDAHPVEPPPIHQKRRIAIVEDNTEVAAALAYALTELGHQVISAASRYEVVTRLNGTAPEI
ILADYRLGGEEDGVGVIRAVRSAFGNDVPALIITGDTDPVVVRRMADHGISVLHKPLDLDALQDRIAALIEARAVSSPHR
TAEGAG

Specific function: Involved in the positive regulation of synthesis of extracellular enzymes and polysaccharide, and hence in pathogenicity. Can restore function to the non-pathogenic mutant 8237 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI145693157, Length=249, Percent_Identity=32.5301204819277, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI87081816, Length=349, Percent_Identity=31.8051575931232, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1789149, Length=306, Percent_Identity=30.718954248366, Blast_Score=125, Evalue=8e-30,
Organism=Escherichia coli, GI48994928, Length=444, Percent_Identity=26.3513513513513, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1788713, Length=410, Percent_Identity=24.390243902439, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1786912, Length=237, Percent_Identity=29.957805907173, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI87082128, Length=221, Percent_Identity=25.7918552036199, Blast_Score=77, Evalue=5e-15,
Organism=Escherichia coli, GI1786783, Length=234, Percent_Identity=25.6410256410256, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1790436, Length=222, Percent_Identity=26.5765765765766, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1786600, Length=231, Percent_Identity=29.8701298701299, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790346, Length=228, Percent_Identity=24.5614035087719, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 71624; Mature: 71624

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS50113 PAC ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSSQTDSLLKAELQQGRALLASVAATTDVMLVYLDPGFNFVWVNEAYARTCRMSPGDMV
CCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCEEEECHHHHHHHCCCCCCCC
GKNHFALYPHAENEAIFRQVRDSGNPVFYKDKPFEFADQQDRGTTYWDWSLAADKDSDGR
CCCCEEEECCCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCCCC
VVGLVFSLRETTGFVRARQTVRETENLLATFIESVPAGIAMFDREMRYLAVSRRFLLDHR
EEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
IIDEDVIGRCHYEVFPDLPERWKAIHRRCLAGETLRCDEDQFVRRNGEINWERWEVRPWF
HCCHHHHCCCCHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCEEEEEEEEEE
TGAGTIGGIILFSENVTDRKRQENGLKAAMEEAERANNAKSRFLAAASHDLRQPLSALSL
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
YVGTLERKLPASERALASGIGDCVTHLSELLSNLLDLSKLEAGVIKPSVCDFEVDAVLLR
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
IALSYEPEAREKGLGLRYTGSAWVARTDPVLFQRVVTNLVANAVRYTEHGGILIGVRRRS
HHHCCCCCHHHCCCCEEECCCEEEECCCHHHHHHHHHHHHHHHHHEECCCCEEEEEEECC
GKHWVEVWDTGIGIPADKTTEIFEEFRQLGNAERNRTKGSGIGLTIAAKTAKLLGLQLQV
CCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHCCCCCCCCEEEEEEHHHHHHEEEEEE
RSRPGRGSVFALELPLGERVDAHPVEPPPIHQKRRIAIVEDNTEVAAALAYALTELGHQV
ECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
ISAASRYEVVTRLNGTAPEIILADYRLGGEEDGVGVIRAVRSAFGNDVPALIITGDTDPV
HHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCHH
VVRRMADHGISVLHKPLDLDALQDRIAALIEARAVSSPHRTAEGAG
HHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNSSQTDSLLKAELQQGRALLASVAATTDVMLVYLDPGFNFVWVNEAYARTCRMSPGDMV
CCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCEEEECHHHHHHHCCCCCCCC
GKNHFALYPHAENEAIFRQVRDSGNPVFYKDKPFEFADQQDRGTTYWDWSLAADKDSDGR
CCCCEEEECCCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCCCC
VVGLVFSLRETTGFVRARQTVRETENLLATFIESVPAGIAMFDREMRYLAVSRRFLLDHR
EEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
IIDEDVIGRCHYEVFPDLPERWKAIHRRCLAGETLRCDEDQFVRRNGEINWERWEVRPWF
HCCHHHHCCCCHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCEEEEEEEEEE
TGAGTIGGIILFSENVTDRKRQENGLKAAMEEAERANNAKSRFLAAASHDLRQPLSALSL
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
YVGTLERKLPASERALASGIGDCVTHLSELLSNLLDLSKLEAGVIKPSVCDFEVDAVLLR
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
IALSYEPEAREKGLGLRYTGSAWVARTDPVLFQRVVTNLVANAVRYTEHGGILIGVRRRS
HHHCCCCCHHHCCCCEEECCCEEEECCCHHHHHHHHHHHHHHHHHEECCCCEEEEEEECC
GKHWVEVWDTGIGIPADKTTEIFEEFRQLGNAERNRTKGSGIGLTIAAKTAKLLGLQLQV
CCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHCCCCCCCCEEEEEEHHHHHHEEEEEE
RSRPGRGSVFALELPLGERVDAHPVEPPPIHQKRRIAIVEDNTEVAAALAYALTELGHQV
ECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
ISAASRYEVVTRLNGTAPEIILADYRLGGEEDGVGVIRAVRSAFGNDVPALIITGDTDPV
HHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCHH
VVRRMADHGISVLHKPLDLDALQDRIAALIEARAVSSPHRTAEGAG
HHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1645442 [H]