Click here to switch to the map view.

The map label for this gene is gatA [H]

Identifier: 119898648

GI number: 119898648

Start: 2596593

End: 2598086

Strand: Reverse

Name: gatA [H]

Synonym: azo2357

Alternate gene names: 119898648

Gene position: 2598086-2596593 (Counterclockwise)

Preceding gene: 119898649

Following gene: 119898641

Centisome position: 59.37

GC content: 72.42

Gene sequence:

>1494_bases
ATGAGCGCGCCGGACTTCCAGCATCTCACCGCCGCGCAGGCGGTCCGCGATCTGCGCGCGGGGCGCTATTCGGCCGAGCA
ACTGGTGCTCGCCTGTCAGGCGCGCATCGACCGCTTCAACCCGACGCTGAACGCGCTGGTGACGCTCAACCGCGAGGGCG
CGCTGGCCGCCGCGCGCGGGGCGGACCGGCGTCTCGCCCAGGGTGGCCTGGCGCCGGCGCTGCTCGGCGTGCCGGTTTCC
ATCAAGGACGCCTTTGCCACCCGGGACATGCTCACCACCGCCAGCCATCGGCCGCTGGCCGCGTACCGGCCGGGGGCCGA
TGCCACGGTGGTTGCGCGCTGGCGCGAGGCCGGCGCGGTGTTGATGGGCAAGAGCAATCTACCGGAGCTGGCGGGCGCGC
CGCACTGCTGGAGCCCGCTGTTCGGCCTCACGCGCAATCCGTGGAACCCGGCGCTCACGCCGGGCGGCAGTTCCGGCGGC
GCCGCGGTGGCCGTGGCCGCGGGATTCTCGCTGCTCGACATCGGCAGCGACATCGCCGGCTCGATCCGCATCCCCGCCGC
CTACTGCGGCATCGCGGGGCTGAAGGCCACCGAGAACCGCATCCCGCGCACCGGCCACATCCCGCACCTGCCGCCGGAAT
TCGGCGGACCGGGCCGCAGCGTCTGGCACATGCTGGCGTTTGGGGTGCTGGCGCGCAGCGTGGAGGACCTGCAACTGGGC
TACGGCCTGCTCGCCGGGCCGGACGGGGTGGATTGCACCGTGCCGCCCTTTCTGCCGGAACCGCCATCGCTGCCGACGAC
GGCCAGGGCGGTCGCGCCGATGCGCATCGCGTTGTGGGACGACTTCGCCGGCATGCCGCTGTGTCCGCGCATCCGGCGCG
GTCTGCAGAACATGGCGGGCAAGCTGCGGGCTAGCGGGCACGAAGTCGTCCGCTGCGCACCGGCGGATTTCGACGTGGGC
GCCGCGTGGCACGCCTTCGGCCTGATCGGCGGGGCGGAAATCGGACTCGGCATGCCGGGCTGGCAGCGCCGGCTCTTCCT
GGCGCTGCGACCGCTGCTGCCGCGCGACCACGTCATCACCCGGGCGGTGGCCGCCGGCATGCGCTTCGATCTGCGCCGCT
ACAACCAGGCGCTGAACCAGCGCGAAGGTCTGATCCGGTCGCTGGAACGCTTTGTGGGCGAGTGGGATGCGGTCATCTGC
CCGGTGGCGCCGACGGGCCCTTATCCCGGCGAGCCGATGCCGCCCACCCGAAAGCCGCCGCGGCTGAACGTGGGCGAGGC
CACACTGCCGTATCTGGAAGCCACCATTGCCATGACCGCGCCGTTCTCGCTGACCGGCAGCCCGGTGTTGAGCCTGCCGG
TGGGCATCGAGGACGGTTTGCCGGTCGGGGTGCAGGTGATCGGCAAGCGCTGGCAGGAACACGCACTGCTGGAAACCGGC
CGCCGGCTGGAAAACGCCCTCGGGGGATTCGTGCCGCCCCCGCTCGCGGCTTGA

Upstream 100 bases:

>100_bases
TACAAGACCCTCCGCGCGCGCAGTTCCTAGACTGGCCCGGTTTTCCACACCGGTATCGCGCCATGCCCAGCCCCCATTCC
TCTGTTTGCCTGTCGCGTCC

Downstream 100 bases:

>100_bases
TCCGGGCGGGGGCGTGGCGGCGGCGGACGCGCCCGCCCGGCGCTACAGCACCTGGCGCAGACCTTCCACGCCCACTGGCG
CCTCGGCCAGCCAGGGCAGC

Product: amidase

Products: monocarboxylate; NH3

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MSAPDFQHLTAAQAVRDLRAGRYSAEQLVLACQARIDRFNPTLNALVTLNREGALAAARGADRRLAQGGLAPALLGVPVS
IKDAFATRDMLTTASHRPLAAYRPGADATVVARWREAGAVLMGKSNLPELAGAPHCWSPLFGLTRNPWNPALTPGGSSGG
AAVAVAAGFSLLDIGSDIAGSIRIPAAYCGIAGLKATENRIPRTGHIPHLPPEFGGPGRSVWHMLAFGVLARSVEDLQLG
YGLLAGPDGVDCTVPPFLPEPPSLPTTARAVAPMRIALWDDFAGMPLCPRIRRGLQNMAGKLRASGHEVVRCAPADFDVG
AAWHAFGLIGGAEIGLGMPGWQRRLFLALRPLLPRDHVITRAVAAGMRFDLRRYNQALNQREGLIRSLERFVGEWDAVIC
PVAPTGPYPGEPMPPTRKPPRLNVGEATLPYLEATIAMTAPFSLTGSPVLSLPVGIEDGLPVGVQVIGKRWQEHALLETG
RRLENALGGFVPPPLAA

Sequences:

>Translated_497_residues
MSAPDFQHLTAAQAVRDLRAGRYSAEQLVLACQARIDRFNPTLNALVTLNREGALAAARGADRRLAQGGLAPALLGVPVS
IKDAFATRDMLTTASHRPLAAYRPGADATVVARWREAGAVLMGKSNLPELAGAPHCWSPLFGLTRNPWNPALTPGGSSGG
AAVAVAAGFSLLDIGSDIAGSIRIPAAYCGIAGLKATENRIPRTGHIPHLPPEFGGPGRSVWHMLAFGVLARSVEDLQLG
YGLLAGPDGVDCTVPPFLPEPPSLPTTARAVAPMRIALWDDFAGMPLCPRIRRGLQNMAGKLRASGHEVVRCAPADFDVG
AAWHAFGLIGGAEIGLGMPGWQRRLFLALRPLLPRDHVITRAVAAGMRFDLRRYNQALNQREGLIRSLERFVGEWDAVIC
PVAPTGPYPGEPMPPTRKPPRLNVGEATLPYLEATIAMTAPFSLTGSPVLSLPVGIEDGLPVGVQVIGKRWQEHALLETG
RRLENALGGFVPPPLAA
>Mature_496_residues
SAPDFQHLTAAQAVRDLRAGRYSAEQLVLACQARIDRFNPTLNALVTLNREGALAAARGADRRLAQGGLAPALLGVPVSI
KDAFATRDMLTTASHRPLAAYRPGADATVVARWREAGAVLMGKSNLPELAGAPHCWSPLFGLTRNPWNPALTPGGSSGGA
AVAVAAGFSLLDIGSDIAGSIRIPAAYCGIAGLKATENRIPRTGHIPHLPPEFGGPGRSVWHMLAFGVLARSVEDLQLGY
GLLAGPDGVDCTVPPFLPEPPSLPTTARAVAPMRIALWDDFAGMPLCPRIRRGLQNMAGKLRASGHEVVRCAPADFDVGA
AWHAFGLIGGAEIGLGMPGWQRRLFLALRPLLPRDHVITRAVAAGMRFDLRRYNQALNQREGLIRSLERFVGEWDAVICP
VAPTGPYPGEPMPPTRKPPRLNVGEATLPYLEATIAMTAPFSLTGSPVLSLPVGIEDGLPVGVQVIGKRWQEHALLETGR
RLENALGGFVPPPLAA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=513, Percent_Identity=25.1461988304094, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI222831590, Length=515, Percent_Identity=26.4077669902913, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI166795287, Length=260, Percent_Identity=34.2307692307692, Blast_Score=91, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17556264, Length=522, Percent_Identity=25.6704980842912, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17537465, Length=521, Percent_Identity=25.7197696737044, Blast_Score=129, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17543272, Length=461, Percent_Identity=26.2472885032538, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17556276, Length=299, Percent_Identity=31.438127090301, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17556278, Length=299, Percent_Identity=31.438127090301, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71990152, Length=239, Percent_Identity=29.7071129707113, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17538252, Length=475, Percent_Identity=25.2631578947368, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17538254, Length=195, Percent_Identity=28.7179487179487, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=415, Percent_Identity=26.0240963855422, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320448, Length=239, Percent_Identity=30.1255230125523, Blast_Score=76, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6323950, Length=456, Percent_Identity=23.9035087719298, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24648113, Length=474, Percent_Identity=28.6919831223629, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24652985, Length=519, Percent_Identity=25.626204238921, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI19922090, Length=519, Percent_Identity=25.626204238921, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24652981, Length=519, Percent_Identity=25.626204238921, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24652983, Length=519, Percent_Identity=25.626204238921, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI45550774, Length=253, Percent_Identity=32.8063241106719, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24648435, Length=253, Percent_Identity=32.8063241106719, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24648437, Length=253, Percent_Identity=32.8063241106719, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI21356731, Length=504, Percent_Identity=26.5873015873016, Blast_Score=115, Evalue=9e-26,
Organism=Drosophila melanogaster, GI161078093, Length=512, Percent_Identity=26.953125, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24644968, Length=247, Percent_Identity=30.7692307692308, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24648441, Length=182, Percent_Identity=34.0659340659341, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648439, Length=182, Percent_Identity=34.0659340659341, Blast_Score=94, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 3.5.1.4

Molecular weight: Translated: 52669; Mature: 52537

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPDFQHLTAAQAVRDLRAGRYSAEQLVLACQARIDRFNPTLNALVTLNREGALAAARG
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEHHCC
ADRRLAQGGLAPALLGVPVSIKDAFATRDMLTTASHRPLAAYRPGADATVVARWREAGAV
CHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCEEEEEHHHCCEE
LMGKSNLPELAGAPHCWSPLFGLTRNPWNPALTPGGSSGGAAVAVAAGFSLLDIGSDIAG
EECCCCCCHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHCCCCCCC
SIRIPAAYCGIAGLKATENRIPRTGHIPHLPPEFGGPGRSVWHMLAFGVLARSVEDLQLG
CEECCHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
YGLLAGPDGVDCTVPPFLPEPPSLPTTARAVAPMRIALWDDFAGMPLCPRIRRGLQNMAG
CCEEECCCCCCCCCCCCCCCCCCCCCHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHH
KLRASGHEVVRCAPADFDVGAAWHAFGLIGGAEIGLGMPGWQRRLFLALRPLLPRDHVIT
HHHCCCCCEEEECCCCCCCCHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHCCCCCHHHHH
RAVAAGMRFDLRRYNQALNQREGLIRSLERFVGEWDAVICPVAPTGPYPGEPMPPTRKPP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCC
RLNVGEATLPYLEATIAMTAPFSLTGSPVLSLPVGIEDGLPVGVQVIGKRWQEHALLETG
CCCCCCCHHHHHHHHHHEECCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
RRLENALGGFVPPPLAA
HHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SAPDFQHLTAAQAVRDLRAGRYSAEQLVLACQARIDRFNPTLNALVTLNREGALAAARG
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEHHCC
ADRRLAQGGLAPALLGVPVSIKDAFATRDMLTTASHRPLAAYRPGADATVVARWREAGAV
CHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCEEEEEHHHCCEE
LMGKSNLPELAGAPHCWSPLFGLTRNPWNPALTPGGSSGGAAVAVAAGFSLLDIGSDIAG
EECCCCCCHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHCCCCCCC
SIRIPAAYCGIAGLKATENRIPRTGHIPHLPPEFGGPGRSVWHMLAFGVLARSVEDLQLG
CEECCHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
YGLLAGPDGVDCTVPPFLPEPPSLPTTARAVAPMRIALWDDFAGMPLCPRIRRGLQNMAG
CCEEECCCCCCCCCCCCCCCCCCCCCHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHH
KLRASGHEVVRCAPADFDVGAAWHAFGLIGGAEIGLGMPGWQRRLFLALRPLLPRDHVIT
HHHCCCCCEEEECCCCCCCCHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHCCCCCHHHHH
RAVAAGMRFDLRRYNQALNQREGLIRSLERFVGEWDAVICPVAPTGPYPGEPMPPTRKPP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCC
RLNVGEATLPYLEATIAMTAPFSLTGSPVLSLPVGIEDGLPVGVQVIGKRWQEHALLETG
CCCCCCCHHHHHHHHHHEECCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
RRLENALGGFVPPPLAA
HHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: monocarboxylic acid amide; H2O

Specific reaction: A monocarboxylic acid amide + H2O = a monocarboxylate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA