Click here to switch to the map view.

The map label for this gene is yhcA [H]

Identifier: 119898594

GI number: 119898594

Start: 2523759

End: 2525189

Strand: Reverse

Name: yhcA [H]

Synonym: azo2303

Alternate gene names: 119898594

Gene position: 2525189-2523759 (Counterclockwise)

Preceding gene: 119898595

Following gene: 119898579

Centisome position: 57.7

GC content: 68.34

Gene sequence:

>1431_bases
ATGCCTTTCCTCCCGCCGCCTTTATCCCGCGACGAACTCCTCGCCCGCCATGGCGAGCGCTACAAGTGGTACGCGCTGAT
CGTCGTCGGCCTCGGCACGATGGCGGCGGTGCTGGCTACCACCAGTTTCAGCGTGGCCGTACCCGCGTTGGGGCAGGCGT
TCGGGATTGGGCAGGAGCGGGTGCAGTGGGCGATCACCGGGTTCATGGCGGCGCTGACGGTGGCGATGTTGCCGACGCCG
TGGCTGCTGGACCGGCTCGGGTTCCGGCGGCTGTTTCTTGGGGCGATTGCGATGCTGGCGCTGACCAGCATTGCGGGGGC
CTTGGCCACCGGCTTTGTATGGGTGGTGGTGGCGCGGGTGCTGCAGGGCGTGGCGGCGGGCTTGTTGCAGCCGTTGTCCA
GCATGGTGGTGATGCGCTTGTTTCCGGCACACAGCCAGGGGCGGGCGTCGGGGCTGCTGGGGTTCGGCATCGTGCTGGCG
CCGGCGGTGGCTCCGACTTTGGGCGGGGTATTGCTGGACCGCTTTGGCTGGCAGGCGATCTTTTTGCTCAGCGTGCCGTT
CTGCGTGGTTGCAGGCGGGCTGGGGTGGGTGCTGCTGCCGCGCGCGGCAGAGCCGGTGCGGCGGCGGTTCGACTGGTACG
GAGTGGGGCTGCTGGGCGTGGCCACGCTGGTGCTGATCGAGTGCGTGGCCAGCCTGCGCCACAGCGGGCTGCTGGCGCCG
TGGACGCTGGCGCAGTTTGCGCTGGTGGCCGTGGCCTGCGGCTTGTTCGTGCGGCATGCGCGGCGGGCGGCGGCGCCCAT
CGTTCATCTCGGTCTGTTTGCCGAGCGCAGCTTTGCGATGGGCACGCTGGTGTGCTTTGCCTACGGTTTCGGGCTGTACG
CCTCCACGTATGTGATTCCGGTGTTCCTGCAGAGCGCGCTGGGCTATCCGGCGACGGACGCGGGCCTGGCGCTATTGCCT
TCCGGGGTGGCGCTGGCGGTGTCGATTCCGTTGGCGGGCCTGTTGTCCGACCGTTATTCGCCGCGTCTGATCACGATTGC
CGGCCTGTTGTTGTTCTGTGCGTCCTTTTTGCTGCTGGCGTGGCGGGGCGGCGGCTTGAGCCATGCGGAGCTGATCGGCT
TCACGGTGATGGGCCGCATCGGCCTCGGCTTGATCATCCCCGCGCTGACGCTGGCGATGCTGCGCCACACGCGCGCGGAA
CTGCTGGGCCAGTCGTCCATGGTGGGCAGCTACACGCGTCAGCTTGGCGGCGTGCTCGGTATCGCGGTGGTGGCGGTGTT
CGTCGCCTGGCGCGAGACGGTGCATGGCCAAGTGCCGCCAGGGTTGTTCGAGGCCTATTCGGAATCCTTCGAACTGCTGG
CGGCGACCTTCATCGTCGCCACGCTGGTGGCAGTGCGGATGAAGCCGCGCGGCCCGTCCGGAACGCGTTAG

Upstream 100 bases:

>100_bases
TTACTGCGCTAACGACGCGTCCCACCCTCCGGGAGAATAGGCAATCCCGAGCCCGCCCCCCGCAGTACCATTCCACCCCG
GCATCCCCGTCACCCCCTCC

Downstream 100 bases:

>100_bases
TCCTGGCGCGTGGCGGCTTCCGGATAAAGCTCGGGCAGCAAGAACTGCAGCGCGTCGAGCGGAAAAGCGAAGCGCACTGG
CGCGACATGGCCGGTGCTGG

Product: hypothetical protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MPFLPPPLSRDELLARHGERYKWYALIVVGLGTMAAVLATTSFSVAVPALGQAFGIGQERVQWAITGFMAALTVAMLPTP
WLLDRLGFRRLFLGAIAMLALTSIAGALATGFVWVVVARVLQGVAAGLLQPLSSMVVMRLFPAHSQGRASGLLGFGIVLA
PAVAPTLGGVLLDRFGWQAIFLLSVPFCVVAGGLGWVLLPRAAEPVRRRFDWYGVGLLGVATLVLIECVASLRHSGLLAP
WTLAQFALVAVACGLFVRHARRAAAPIVHLGLFAERSFAMGTLVCFAYGFGLYASTYVIPVFLQSALGYPATDAGLALLP
SGVALAVSIPLAGLLSDRYSPRLITIAGLLLFCASFLLLAWRGGGLSHAELIGFTVMGRIGLGLIIPALTLAMLRHTRAE
LLGQSSMVGSYTRQLGGVLGIAVVAVFVAWRETVHGQVPPGLFEAYSESFELLAATFIVATLVAVRMKPRGPSGTR

Sequences:

>Translated_476_residues
MPFLPPPLSRDELLARHGERYKWYALIVVGLGTMAAVLATTSFSVAVPALGQAFGIGQERVQWAITGFMAALTVAMLPTP
WLLDRLGFRRLFLGAIAMLALTSIAGALATGFVWVVVARVLQGVAAGLLQPLSSMVVMRLFPAHSQGRASGLLGFGIVLA
PAVAPTLGGVLLDRFGWQAIFLLSVPFCVVAGGLGWVLLPRAAEPVRRRFDWYGVGLLGVATLVLIECVASLRHSGLLAP
WTLAQFALVAVACGLFVRHARRAAAPIVHLGLFAERSFAMGTLVCFAYGFGLYASTYVIPVFLQSALGYPATDAGLALLP
SGVALAVSIPLAGLLSDRYSPRLITIAGLLLFCASFLLLAWRGGGLSHAELIGFTVMGRIGLGLIIPALTLAMLRHTRAE
LLGQSSMVGSYTRQLGGVLGIAVVAVFVAWRETVHGQVPPGLFEAYSESFELLAATFIVATLVAVRMKPRGPSGTR
>Mature_475_residues
PFLPPPLSRDELLARHGERYKWYALIVVGLGTMAAVLATTSFSVAVPALGQAFGIGQERVQWAITGFMAALTVAMLPTPW
LLDRLGFRRLFLGAIAMLALTSIAGALATGFVWVVVARVLQGVAAGLLQPLSSMVVMRLFPAHSQGRASGLLGFGIVLAP
AVAPTLGGVLLDRFGWQAIFLLSVPFCVVAGGLGWVLLPRAAEPVRRRFDWYGVGLLGVATLVLIECVASLRHSGLLAPW
TLAQFALVAVACGLFVRHARRAAAPIVHLGLFAERSFAMGTLVCFAYGFGLYASTYVIPVFLQSALGYPATDAGLALLPS
GVALAVSIPLAGLLSDRYSPRLITIAGLLLFCASFLLLAWRGGGLSHAELIGFTVMGRIGLGLIIPALTLAMLRHTRAEL
LGQSSMVGSYTRQLGGVLGIAVVAVFVAWRETVHGQVPPGLFEAYSESFELLAATFIVATLVAVRMKPRGPSGTR

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=421, Percent_Identity=27.0783847980998, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI1790195, Length=444, Percent_Identity=28.8288288288288, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1788710, Length=447, Percent_Identity=26.8456375838926, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1788392, Length=303, Percent_Identity=29.7029702970297, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI87081983, Length=415, Percent_Identity=24.578313253012, Blast_Score=96, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50298; Mature: 50166

Theoretical pI: Translated: 10.65; Mature: 10.65

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFLPPPLSRDELLARHGERYKWYALIVVGLGTMAAVLATTSFSVAVPALGQAFGIGQER
CCCCCCCCCHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHH
VQWAITGFMAALTVAMLPTPWLLDRLGFRRLFLGAIAMLALTSIAGALATGFVWVVVARV
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQGVAAGLLQPLSSMVVMRLFPAHSQGRASGLLGFGIVLAPAVAPTLGGVLLDRFGWQAI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
FLLSVPFCVVAGGLGWVLLPRAAEPVRRRFDWYGVGLLGVATLVLIECVASLRHSGLLAP
HHHHHHHHHHHCCCHHEECCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCH
WTLAQFALVAVACGLFVRHARRAAAPIVHLGLFAERSFAMGTLVCFAYGFGLYASTYVIP
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VFLQSALGYPATDAGLALLPSGVALAVSIPLAGLLSDRYSPRLITIAGLLLFCASFLLLA
HHHHHHCCCCCCCCCHHHCCCCCEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
WRGGGLSHAELIGFTVMGRIGLGLIIPALTLAMLRHTRAELLGQSSMVGSYTRQLGGVLG
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
IAVVAVFVAWRETVHGQVPPGLFEAYSESFELLAATFIVATLVAVRMKPRGPSGTR
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PFLPPPLSRDELLARHGERYKWYALIVVGLGTMAAVLATTSFSVAVPALGQAFGIGQER
CCCCCCCCHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHH
VQWAITGFMAALTVAMLPTPWLLDRLGFRRLFLGAIAMLALTSIAGALATGFVWVVVARV
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQGVAAGLLQPLSSMVVMRLFPAHSQGRASGLLGFGIVLAPAVAPTLGGVLLDRFGWQAI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
FLLSVPFCVVAGGLGWVLLPRAAEPVRRRFDWYGVGLLGVATLVLIECVASLRHSGLLAP
HHHHHHHHHHHCCCHHEECCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCH
WTLAQFALVAVACGLFVRHARRAAAPIVHLGLFAERSFAMGTLVCFAYGFGLYASTYVIP
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VFLQSALGYPATDAGLALLPSGVALAVSIPLAGLLSDRYSPRLITIAGLLLFCASFLLLA
HHHHHHCCCCCCCCCHHHCCCCCEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
WRGGGLSHAELIGFTVMGRIGLGLIIPALTLAMLRHTRAELLGQSSMVGSYTRQLGGVLG
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
IAVVAVFVAWRETVHGQVPPGLFEAYSESFELLAATFIVATLVAVRMKPRGPSGTR
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 8969498 [H]