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The map label for this gene is gspE3 [H]

Identifier: 119898592

GI number: 119898592

Start: 2520561

End: 2522381

Strand: Direct

Name: gspE3 [H]

Synonym: azo2301

Alternate gene names: 119898592

Gene position: 2520561-2522381 (Clockwise)

Preceding gene: 119898591

Following gene: 119898593

Centisome position: 57.6

GC content: 68.15

Gene sequence:

>1821_bases
ATGACAGCCCTTCACTCCCCCACCGCCAGTGCGCCCCCGGCGCACGCCCGCAACACGAGCGCTTTCGAACACCGCCTGAC
CGCCGACGAAGTCCTGGAAGGACTGCTCGCCGAAGGCCAGATCAGCCGCAAGGACCACGACGACACCCAGGCCATCCTGC
GCCTGCGCACGCGTGAGATGCATCCCTTGCAGCAGATCGGCGAACGCCGCCTCACCCGGGCGCTGCCGCCGCACGACGTG
CTGACCACCGAGGCGCTGACCGAATGGCTGGGGGGCCGCAGCGGCGTGCCCTACTTCCACATCGACCCGCTCAAGATCGA
CGTCACCACCGTGTGCGGGCTGGTGTCGCACGCCTATGCCACCCGCTTCGGCATCCTGCCGGTGGCCGTGCAGGGCGACG
AGGTCACCATCGCCACCGCGGAACCGTACGAAACCGAGTGGGTGACGGTGCTGGAGCAGTTGCTGCGCATCCGCATCCGC
CGCGTGCTTTCCAACCCGACCGACGTCGTCCGCTACCTCGCCGAGTTCTACAGCCTGGCGCGCTCGCTGAAGCAGGCCAC
CGACAAGCACCAGGACCTGCCCACCGGCATCGGCAACTTCGAGCAGCTGGTGCAGCTCGGCAGCAAGAAGTCGCTCGACG
CCAACGACCAGGCGATCGTGCACATCGTCGATTGGCTGCTGCAGTACGCCTTCGAACAGCGCGCGTCCGACATCCACATG
GAGCCGCGCCGCGACATCTGCAACGTGCGCTTCCGCATCGACGGCGTGATGCACCAGGTGTATCAGGCACCGCCGCAGGT
GATGGCCGCCATCACCAATCGCGTGAAGCTGATGGGCCGCATGGACGTGGTGGAAAAACGCCGCCCGCAGGACGGCCGCA
TCCGCACGCGCTCGCCCAAGGGCGAGGAAATCGAGTTGCGCCTGTCCACCATGCCCACCGCCTTCGGCGAAAAGCTGGTG
CTGCGCATCTTCGACCCCAGCCTGCAGCTCAAGTCCTTCGCCGACCTCGGTTTCGGCCGCGAGGAAGAATCGCGCTGGCG
GCGGCTCTACCAGCAACCGCACGGCATCATCCTCGTCACCGGCCCCACCGGCTCGGGCAAGACCACCACGCTGTACTCCA
CGCTGAAGGAGATCGCCACGCCCGAGATGAACGTGTGCACGGTGGAGGACCCGATCGAGATGATCTATCCCTACCTCAAC
CAGATGCAGGTGCAGCACGGCATCGGGCTGGACTTTGCCGCCGGCGTGCGCACCCTGCTGCGGCAGGACCCGGACGTGAT
CATGGTGGGCGAAATCCGCGACCTCGAAACCGCCGAGATGGCGGTGCAGGCCGCGCTGACCGGCCACCTGGTGCTCTCCA
CGCTGCACACCAACGGCGCCCCGGCCGCCATCACCCGCCTGATGGACCTCGGCGTGCCGCCCTACCTCATCCGCTCCAGC
CTGCTCGGCGTGCTCGCCCAGCGTCTTGCGCGCACGCTGTGCCCGCACTGCAAGGAACCCGCGCCCCCGCCCGAGGACGA
GTGGAACGAACTCGTCAGCCCGTGGAAGGCCCCGGTGCCGCAACAGGCCAAGGGCCCCAAGGGCTGCCTCGAATGCCGCA
TGACCGGTTACCTCGGCCGCATCAGCATCCACGAGATGCTGGTGATGACGCCCACGCTGCGCCGCATGGTGCAGCCCGGC
ATGGACATCGCCCCCTTCCGCGAACAAGCCGTGCGCGAAGGCATGCAGCCGCTGCGCCTGGCCGGCGCCCGCCACATCGC
CGCGGGCATCACCACGGCGGAGGAAATCGCCCGCTGCACGCCGCCGGCGAATGAGGCGTGA

Upstream 100 bases:

>100_bases
TCTTTCCTGATCCGCTCGTCATCGCGGCAATGTATCGGCGCCGGCACTTGAGCCGCGGCCGCCCCTTGCTATAGCCTGCC
GGGATATCCGCGCGACCGCC

Downstream 100 bases:

>100_bases
GGCCAGGAATACCCCACACAAGCGCCGGCGAAAGAACCGGCAAAAAATACCCACACCAACAGGCAAATCGGGCCTGCAAT
GGCGTACAAGTCATTATTAA

Product: general secretory pathway protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 606; Mature: 605

Protein sequence:

>606_residues
MTALHSPTASAPPAHARNTSAFEHRLTADEVLEGLLAEGQISRKDHDDTQAILRLRTREMHPLQQIGERRLTRALPPHDV
LTTEALTEWLGGRSGVPYFHIDPLKIDVTTVCGLVSHAYATRFGILPVAVQGDEVTIATAEPYETEWVTVLEQLLRIRIR
RVLSNPTDVVRYLAEFYSLARSLKQATDKHQDLPTGIGNFEQLVQLGSKKSLDANDQAIVHIVDWLLQYAFEQRASDIHM
EPRRDICNVRFRIDGVMHQVYQAPPQVMAAITNRVKLMGRMDVVEKRRPQDGRIRTRSPKGEEIELRLSTMPTAFGEKLV
LRIFDPSLQLKSFADLGFGREEESRWRRLYQQPHGIILVTGPTGSGKTTTLYSTLKEIATPEMNVCTVEDPIEMIYPYLN
QMQVQHGIGLDFAAGVRTLLRQDPDVIMVGEIRDLETAEMAVQAALTGHLVLSTLHTNGAPAAITRLMDLGVPPYLIRSS
LLGVLAQRLARTLCPHCKEPAPPPEDEWNELVSPWKAPVPQQAKGPKGCLECRMTGYLGRISIHEMLVMTPTLRRMVQPG
MDIAPFREQAVREGMQPLRLAGARHIAAGITTAEEIARCTPPANEA

Sequences:

>Translated_606_residues
MTALHSPTASAPPAHARNTSAFEHRLTADEVLEGLLAEGQISRKDHDDTQAILRLRTREMHPLQQIGERRLTRALPPHDV
LTTEALTEWLGGRSGVPYFHIDPLKIDVTTVCGLVSHAYATRFGILPVAVQGDEVTIATAEPYETEWVTVLEQLLRIRIR
RVLSNPTDVVRYLAEFYSLARSLKQATDKHQDLPTGIGNFEQLVQLGSKKSLDANDQAIVHIVDWLLQYAFEQRASDIHM
EPRRDICNVRFRIDGVMHQVYQAPPQVMAAITNRVKLMGRMDVVEKRRPQDGRIRTRSPKGEEIELRLSTMPTAFGEKLV
LRIFDPSLQLKSFADLGFGREEESRWRRLYQQPHGIILVTGPTGSGKTTTLYSTLKEIATPEMNVCTVEDPIEMIYPYLN
QMQVQHGIGLDFAAGVRTLLRQDPDVIMVGEIRDLETAEMAVQAALTGHLVLSTLHTNGAPAAITRLMDLGVPPYLIRSS
LLGVLAQRLARTLCPHCKEPAPPPEDEWNELVSPWKAPVPQQAKGPKGCLECRMTGYLGRISIHEMLVMTPTLRRMVQPG
MDIAPFREQAVREGMQPLRLAGARHIAAGITTAEEIARCTPPANEA
>Mature_605_residues
TALHSPTASAPPAHARNTSAFEHRLTADEVLEGLLAEGQISRKDHDDTQAILRLRTREMHPLQQIGERRLTRALPPHDVL
TTEALTEWLGGRSGVPYFHIDPLKIDVTTVCGLVSHAYATRFGILPVAVQGDEVTIATAEPYETEWVTVLEQLLRIRIRR
VLSNPTDVVRYLAEFYSLARSLKQATDKHQDLPTGIGNFEQLVQLGSKKSLDANDQAIVHIVDWLLQYAFEQRASDIHME
PRRDICNVRFRIDGVMHQVYQAPPQVMAAITNRVKLMGRMDVVEKRRPQDGRIRTRSPKGEEIELRLSTMPTAFGEKLVL
RIFDPSLQLKSFADLGFGREEESRWRRLYQQPHGIILVTGPTGSGKTTTLYSTLKEIATPEMNVCTVEDPIEMIYPYLNQ
MQVQHGIGLDFAAGVRTLLRQDPDVIMVGEIRDLETAEMAVQAALTGHLVLSTLHTNGAPAAITRLMDLGVPPYLIRSSL
LGVLAQRLARTLCPHCKEPAPPPEDEWNELVSPWKAPVPQQAKGPKGCLECRMTGYLGRISIHEMLVMTPTLRRMVQPGM
DIAPFREQAVREGMQPLRLAGARHIAAGITTAEEIARCTPPANEA

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=473, Percent_Identity=41.6490486257928, Blast_Score=335, Evalue=4e-93,
Organism=Escherichia coli, GI1786296, Length=376, Percent_Identity=43.6170212765958, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI87082188, Length=216, Percent_Identity=34.7222222222222, Blast_Score=115, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 67675; Mature: 67544

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALHSPTASAPPAHARNTSAFEHRLTADEVLEGLLAEGQISRKDHDDTQAILRLRTREM
CCCCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHC
HPLQQIGERRLTRALPPHDVLTTEALTEWLGGRSGVPYFHIDPLKIDVTTVCGLVSHAYA
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCEEHHHHHHHHHHHHHH
TRFGILPVAVQGDEVTIATAEPYETEWVTVLEQLLRIRIRRVLSNPTDVVRYLAEFYSLA
HHHCCEEEEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
RSLKQATDKHQDLPTGIGNFEQLVQLGSKKSLDANDQAIVHIVDWLLQYAFEQRASDIHM
HHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
EPRRDICNVRFRIDGVMHQVYQAPPQVMAAITNRVKLMGRMDVVEKRRPQDGRIRTRSPK
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCC
GEEIELRLSTMPTAFGEKLVLRIFDPSLQLKSFADLGFGREEESRWRRLYQQPHGIILVT
CCEEEEEEECCCCHHHHHHEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEE
GPTGSGKTTTLYSTLKEIATPEMNVCTVEDPIEMIYPYLNQMQVQHGIGLDFAAGVRTLL
CCCCCCCHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
RQDPDVIMVGEIRDLETAEMAVQAALTGHLVLSTLHTNGAPAAITRLMDLGVPPYLIRSS
HCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHH
LLGVLAQRLARTLCPHCKEPAPPPEDEWNELVSPWKAPVPQQAKGPKGCLECRMTGYLGR
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCHHH
ISIHEMLVMTPTLRRMVQPGMDIAPFREQAVREGMQPLRLAGARHIAAGITTAEEIARCT
HHHHHHHHHCHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHCC
PPANEA
CCCCCC
>Mature Secondary Structure 
TALHSPTASAPPAHARNTSAFEHRLTADEVLEGLLAEGQISRKDHDDTQAILRLRTREM
CCCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHC
HPLQQIGERRLTRALPPHDVLTTEALTEWLGGRSGVPYFHIDPLKIDVTTVCGLVSHAYA
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCEEHHHHHHHHHHHHHH
TRFGILPVAVQGDEVTIATAEPYETEWVTVLEQLLRIRIRRVLSNPTDVVRYLAEFYSLA
HHHCCEEEEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
RSLKQATDKHQDLPTGIGNFEQLVQLGSKKSLDANDQAIVHIVDWLLQYAFEQRASDIHM
HHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
EPRRDICNVRFRIDGVMHQVYQAPPQVMAAITNRVKLMGRMDVVEKRRPQDGRIRTRSPK
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCC
GEEIELRLSTMPTAFGEKLVLRIFDPSLQLKSFADLGFGREEESRWRRLYQQPHGIILVT
CCEEEEEEECCCCHHHHHHEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEE
GPTGSGKTTTLYSTLKEIATPEMNVCTVEDPIEMIYPYLNQMQVQHGIGLDFAAGVRTLL
CCCCCCCHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
RQDPDVIMVGEIRDLETAEMAVQAALTGHLVLSTLHTNGAPAAITRLMDLGVPPYLIRSS
HCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHH
LLGVLAQRLARTLCPHCKEPAPPPEDEWNELVSPWKAPVPQQAKGPKGCLECRMTGYLGR
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCHHH
ISIHEMLVMTPTLRRMVQPGMDIAPFREQAVREGMQPLRLAGARHIAAGITTAEEIARCT
HHHHHHHHHCHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHCC
PPANEA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8820654 [H]