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The map label for this gene is alkB [H]

Identifier: 119898584

GI number: 119898584

Start: 2512299

End: 2512964

Strand: Direct

Name: alkB [H]

Synonym: azo2293

Alternate gene names: 119898584

Gene position: 2512299-2512964 (Clockwise)

Preceding gene: 119898583

Following gene: 119898585

Centisome position: 57.41

GC content: 73.57

Gene sequence:

>666_bases
ATGACGCCCGACCTCTTCTCCACCCTTGCGCCACAGCCCGCCCGCGTCACCCTGGTGCCGCTGGCACCGGGCGCGGTGCT
GCTGCGCCAGCGCGCGCTCGCGCAGGCAGAAGCGTTGCTCGCCGGCGTGGCAGAGGTGACAGCGGTCGCGCCCTTTCGCT
GGATGTCCACGCCCAACGGCTACCGCATGTCGGTGGCGATGACCAACTGCGGCGCCCTCGGCTGGGTGTCCGACGCCACC
GGCTACCGCTACGCGCCACTGGACCCGGCGCGGGACGCGCCCTGGCCGGCGATGCCCGCCGCCTTCCTTGAGCTTGCCCG
GCAAGCCGCGGCCGACGCCGGTTTTCCCGCCTTCGAGCCGGACGCCTGCCTGGTCAATCGCTACGCGCCCGGCGCCCGCC
TCAGCCTGCACCAGGACCGCAACGAGCACGACTTTTCCCAGCCCATCGTGTCTGTCTCGCTCGGCCTGCCGGCGGCGTTC
CAGTTCGGCGGGCTGCGGCGCGGCGATCCGGTGACCCGCTACCTGCTGGAGCACGGCGACGTGGTGGTATGGGGCGGCCC
CGCGCGCCTGCGCTACCACGGCGTGCTGGCGCTGAAGGACGGCGAGCACGCCGCGACCGGCGCCTGCCGCATCAACCTCA
CCTTCCGCCGCGCCGGCCGGGTTTGA

Upstream 100 bases:

>100_bases
AACGCGCGCTGCTCGCACGCGAGCGCGCCGCCCGCACGGAAACCGATCTCTTCGCGGCCGACACGCAATCCCCGGCCGAG
CGCTGAAGCCCGCCCTTGCG

Downstream 100 bases:

>100_bases
TTTTCGCCTGCGCCTGACCCATGGTTCATTAGGCCGCGGCATCGTCCGCGCGCCGTGTCCCCTTACGCCGTACCGGAGCC
CGGATGCCTCTGAGCGCCTC

Product: DNA-directed DNA polymerase

Products: NA

Alternate protein names: Alkylated DNA repair protein AlkB [H]

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MTPDLFSTLAPQPARVTLVPLAPGAVLLRQRALAQAEALLAGVAEVTAVAPFRWMSTPNGYRMSVAMTNCGALGWVSDAT
GYRYAPLDPARDAPWPAMPAAFLELARQAAADAGFPAFEPDACLVNRYAPGARLSLHQDRNEHDFSQPIVSVSLGLPAAF
QFGGLRRGDPVTRYLLEHGDVVVWGGPARLRYHGVLALKDGEHAATGACRINLTFRRAGRV

Sequences:

>Translated_221_residues
MTPDLFSTLAPQPARVTLVPLAPGAVLLRQRALAQAEALLAGVAEVTAVAPFRWMSTPNGYRMSVAMTNCGALGWVSDAT
GYRYAPLDPARDAPWPAMPAAFLELARQAAADAGFPAFEPDACLVNRYAPGARLSLHQDRNEHDFSQPIVSVSLGLPAAF
QFGGLRRGDPVTRYLLEHGDVVVWGGPARLRYHGVLALKDGEHAATGACRINLTFRRAGRV
>Mature_220_residues
TPDLFSTLAPQPARVTLVPLAPGAVLLRQRALAQAEALLAGVAEVTAVAPFRWMSTPNGYRMSVAMTNCGALGWVSDATG
YRYAPLDPARDAPWPAMPAAFLELARQAAADAGFPAFEPDACLVNRYAPGARLSLHQDRNEHDFSQPIVSVSLGLPAAFQ
FGGLRRGDPVTRYLLEHGDVVVWGGPARLRYHGVLALKDGEHAATGACRINLTFRRAGRV

Specific function: Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable a

COG id: COG3145

COG function: function code L; Alkylated DNA repair protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Homo sapiens, GI87298840, Length=123, Percent_Identity=34.1463414634146, Blast_Score=71, Evalue=8e-13,
Organism=Escherichia coli, GI1788541, Length=217, Percent_Identity=59.4470046082949, Blast_Score=264, Evalue=3e-72,
Organism=Drosophila melanogaster, GI45555401, Length=119, Percent_Identity=34.453781512605, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004574 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 23685; Mature: 23554

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPDLFSTLAPQPARVTLVPLAPGAVLLRQRALAQAEALLAGVAEVTAVAPFRWMSTPNG
CCCHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCC
YRMSVAMTNCGALGWVSDATGYRYAPLDPARDAPWPAMPAAFLELARQAAADAGFPAFEP
EEEEEEEECCCCCCCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
DACLVNRYAPGARLSLHQDRNEHDFSQPIVSVSLGLPAAFQFGGLRRGDPVTRYLLEHGD
CHHEEECCCCCCEEEECCCCCCCCHHCCEEEEECCCCCHHHCCCCCCCCHHHHHHHHCCC
VVVWGGPARLRYHGVLALKDGEHAATGACRINLTFRRAGRV
EEEECCCCEEEEEEEEEEECCCCCCCCEEEEEEEEECCCCC
>Mature Secondary Structure 
TPDLFSTLAPQPARVTLVPLAPGAVLLRQRALAQAEALLAGVAEVTAVAPFRWMSTPNG
CCHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCC
YRMSVAMTNCGALGWVSDATGYRYAPLDPARDAPWPAMPAAFLELARQAAADAGFPAFEP
EEEEEEEECCCCCCCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
DACLVNRYAPGARLSLHQDRNEHDFSQPIVSVSLGLPAAFQFGGLRRGDPVTRYLLEHGD
CHHEEECCCCCCEEEECCCCCCCCHHCCEEEEECCCCCHHHCCCCCCCCHHHHHHHHCCC
VVVWGGPARLRYHGVLALKDGEHAATGACRINLTFRRAGRV
EEEECCCCEEEEEEEEEEECCCCCCCCEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536913; 9097040; 9278503; 3887409; 7928996 [H]