Click here to switch to the map view.

The map label for this gene is ada [H]

Identifier: 119898583

GI number: 119898583

Start: 2511136

End: 2512284

Strand: Direct

Name: ada [H]

Synonym: azo2292

Alternate gene names: 119898583

Gene position: 2511136-2512284 (Clockwise)

Preceding gene: 119898582

Following gene: 119898584

Centisome position: 57.38

GC content: 73.11

Gene sequence:

>1149_bases
ATGAACTCCGCAGCTTCAGTGCTCCCTACAGTCGAACCCGCCCGCACGGGGCCGGCCGCCGAACACGATCCGCGCTGGGC
CGCCGTACGGACGCGCGATGCCAGCGCGGACGGCCAGTTCTACTACGCCGTGCGCACGACCGGCGTGTATTGCCGCCCCT
CCTGCCCGTCGCGTACGCCGCGGCCCGAAAACGTCAGCTTCCACGCCAGCCGCGAAGACGCCGAACGCGCGGGCTATCGC
CCTTGCCGCCGCTGCCGGCCGGACGCGCCGTCGCTGGAAGAACGCCAGCGTGCGCTCGTCACCGCCGCCTGCCGGGCCAT
CGAGGATGCCGCCGGACCGCCGCCGCTGGCGGAGCTGGCGGCCCAGGCCGGGCTCAGCAGCTCGCGCTTTCACCGCATCT
TCCGCGCCCACACCGGCCTTACCCCGCGCGCCTACGCCCAGGCGATACGCGCGGCGCGCAGCCGCAGCCTGCTGGGCGAC
GGGGAAGCGGCCACCGATACCGCCTACGCAGCCGGCTACAACAGCGCCAGCCGATTCTATGCGGATGCCACTCCGATGCT
GGGGATGGCCCCGTCGCGCTTTCGCAACGGCGGCGACGGCATCGAGATCCGCTTCGCGGTGGGCGCCTGCACGCTGGGCG
ACATCCTCGTCGCCGCCACCGCGCAGGGCATCTGCGCCATCCTGCTCGGCGACGATGCGGACGCGCTGCTGCAGGACCTC
CAGCACCGCTTTCCGCGTGCGCAACTGGTGGGTGGCGACGCCACCTTCGAGCACACCGTGGCCTGCGTGGTCGGTCTGGT
CGAGGCGCCCGCCATCGGGCTGGACCTGCCGCTCGACATCCGCGGCACCGCGTTCCAGCAGCGCGTGTGGGAAGCCCTGC
GCCGCATCCCCGCGGGCAGCACCGCAAGCTACGCGGAAATCGCCGAACGCATCGGCGCACCCGCCGCGGTGCGCGCGGTG
GCCGGTGCCTGCGCCGCCAATCCGATTGCGGTGGCGATCCCCTGCCATCGCGTGGTGCGCAGCGACGGCGGACTGTCGGG
CTACCGCTGGGGCGTCGAACGCAAACGCGCGCTGCTCGCACGCGAGCGCGCCGCCCGCACGGAAACCGATCTCTTCGCGG
CCGACACGCAATCCCCGGCCGAGCGCTGA

Upstream 100 bases:

>100_bases
CCCCGCCTCGGCGTCGAATTCGGCAGCAAGCTTCGGGATGCGCGGACCTCGGCCCGCCGCTAGAGTGTCCCCAGCGTTCA
GCCCTTCGAGATCCGGCAAC

Downstream 100 bases:

>100_bases
AGCCCGCCCTTGCGATGACGCCCGACCTCTTCTCCACCCTTGCGCCACAGCCCGCCCGCGTCACCCTGGTGCCGCTGGCA
CCGGGCGCGGTGCTGCTGCG

Product: methylated-DNA--[protein]-cysteine S-methyltransferase

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MNSAASVLPTVEPARTGPAAEHDPRWAAVRTRDASADGQFYYAVRTTGVYCRPSCPSRTPRPENVSFHASREDAERAGYR
PCRRCRPDAPSLEERQRALVTAACRAIEDAAGPPPLAELAAQAGLSSSRFHRIFRAHTGLTPRAYAQAIRAARSRSLLGD
GEAATDTAYAAGYNSASRFYADATPMLGMAPSRFRNGGDGIEIRFAVGACTLGDILVAATAQGICAILLGDDADALLQDL
QHRFPRAQLVGGDATFEHTVACVVGLVEAPAIGLDLPLDIRGTAFQQRVWEALRRIPAGSTASYAEIAERIGAPAAVRAV
AGACAANPIAVAIPCHRVVRSDGGLSGYRWGVERKRALLARERAARTETDLFAADTQSPAER

Sequences:

>Translated_382_residues
MNSAASVLPTVEPARTGPAAEHDPRWAAVRTRDASADGQFYYAVRTTGVYCRPSCPSRTPRPENVSFHASREDAERAGYR
PCRRCRPDAPSLEERQRALVTAACRAIEDAAGPPPLAELAAQAGLSSSRFHRIFRAHTGLTPRAYAQAIRAARSRSLLGD
GEAATDTAYAAGYNSASRFYADATPMLGMAPSRFRNGGDGIEIRFAVGACTLGDILVAATAQGICAILLGDDADALLQDL
QHRFPRAQLVGGDATFEHTVACVVGLVEAPAIGLDLPLDIRGTAFQQRVWEALRRIPAGSTASYAEIAERIGAPAAVRAV
AGACAANPIAVAIPCHRVVRSDGGLSGYRWGVERKRALLARERAARTETDLFAADTQSPAER
>Mature_382_residues
MNSAASVLPTVEPARTGPAAEHDPRWAAVRTRDASADGQFYYAVRTTGVYCRPSCPSRTPRPENVSFHASREDAERAGYR
PCRRCRPDAPSLEERQRALVTAACRAIEDAAGPPPLAELAAQAGLSSSRFHRIFRAHTGLTPRAYAQAIRAARSRSLLGD
GEAATDTAYAAGYNSASRFYADATPMLGMAPSRFRNGGDGIEIRFAVGACTLGDILVAATAQGICAILLGDDADALLQDL
QHRFPRAQLVGGDATFEHTVACVVGLVEAPAIGLDLPLDIRGTAFQQRVWEALRRIPAGSTASYAEIAERIGAPAAVRAV
AGACAANPIAVAIPCHRVVRSDGGLSGYRWGVERKRALLARERAARTETDLFAADTQSPAER

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=95, Percent_Identity=38.9473684210526, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1788542, Length=340, Percent_Identity=50.2941176470588, Blast_Score=343, Evalue=1e-95,
Organism=Escherichia coli, GI1787596, Length=87, Percent_Identity=47.1264367816092, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI115533070, Length=133, Percent_Identity=42.1052631578947, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI115533068, Length=133, Percent_Identity=42.1052631578947, Blast_Score=102, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6320001, Length=84, Percent_Identity=42.8571428571429, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI17137554, Length=179, Percent_Identity=34.6368715083799, Blast_Score=85, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 40605; Mature: 40605

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSAASVLPTVEPARTGPAAEHDPRWAAVRTRDASADGQFYYAVRTTGVYCRPSCPSRTP
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEECCEEECCCCCCCCC
RPENVSFHASREDAERAGYRPCRRCRPDAPSLEERQRALVTAACRAIEDAAGPPPLAELA
CCCCEEEECCCHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
AQAGLSSSRFHRIFRAHTGLTPRAYAQAIRAARSRSLLGDGEAATDTAYAAGYNSASRFY
HHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCHHEE
ADATPMLGMAPSRFRNGGDGIEIRFAVGACTLGDILVAATAQGICAILLGDDADALLQDL
CCCCCCCCCCHHHHCCCCCCEEEEEEECCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHH
QHRFPRAQLVGGDATFEHTVACVVGLVEAPAIGLDLPLDIRGTAFQQRVWEALRRIPAGS
HHHCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
TASYAEIAERIGAPAAVRAVAGACAANPIAVAIPCHRVVRSDGGLSGYRWGVERKRALLA
CCHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECHHHHHHCCCCCCHHHHHHHHHHHHHH
RERAARTETDLFAADTQSPAER
HHHHHHCHHHHEECCCCCCCCC
>Mature Secondary Structure
MNSAASVLPTVEPARTGPAAEHDPRWAAVRTRDASADGQFYYAVRTTGVYCRPSCPSRTP
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEECCEEECCCCCCCCC
RPENVSFHASREDAERAGYRPCRRCRPDAPSLEERQRALVTAACRAIEDAAGPPPLAELA
CCCCEEEECCCHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
AQAGLSSSRFHRIFRAHTGLTPRAYAQAIRAARSRSLLGDGEAATDTAYAAGYNSASRFY
HHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCHHEE
ADATPMLGMAPSRFRNGGDGIEIRFAVGACTLGDILVAATAQGICAILLGDDADALLQDL
CCCCCCCCCCHHHHCCCCCCEEEEEEECCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHH
QHRFPRAQLVGGDATFEHTVACVVGLVEAPAIGLDLPLDIRGTAFQQRVWEALRRIPAGS
HHHCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
TASYAEIAERIGAPAAVRAVAGACAANPIAVAIPCHRVVRSDGGLSGYRWGVERKRALLA
CCHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECHHHHHHCCCCCCHHHHHHHHHHHHHH
RERAARTETDLFAADTQSPAER
HHHHHHCHHHHEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]