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The map label for this gene is nifF2 [H]

Identifier: 119898572

GI number: 119898572

Start: 2499793

End: 2500329

Strand: Direct

Name: nifF2 [H]

Synonym: azo2281

Alternate gene names: 119898572

Gene position: 2499793-2500329 (Clockwise)

Preceding gene: 119898571

Following gene: 119898573

Centisome position: 57.12

GC content: 66.67

Gene sequence:

>537_bases
ATGGCAAAAATCGGCATTTTCTTCGGCAGCGACACCGGCCGCACGCGCAAGGTCGCCAAGTACATCGCCAAGAAGCTCGG
CGAAGTGGCGGCGGACCCGGTCAACGTCAACAAGGCCACGGTCGAGGACTTCCTCGCCTACGACGCCCTGATCATCGGCA
GCCCGACGCTGGGCGACGGTCAGTTGCCGGGGCTGGAATCGGGCGCGCAGAACGAGAGCTGGGCCGAATTCCTGCCCCAG
CTCGACGGCGCCGACATGAGTGGCAAGGTGGTGGCGATCTACGGCCTGGGCGACCAGGACAAGTACGCCAACGAGTTCTG
CGACGCGATCGGCCTGCTCTACGACGCGGTCACCACCTGCGGCGCCACCGTGGTGGGGCCGTGGCCGACCGAGGGCTACA
GTTTCAAGAGCTCGCAGGCGGTCATCGACGGCCAGTTCTGCGGCCTTGCGCTGGACAACGACAACCAGGCCGGCCTCACC
GACGAGCGCGTCGACACCTGGCTGGAGCAGGTCAAGCCGGCACTGCTCGGCGCCTGA

Upstream 100 bases:

>100_bases
GCATGAAGCGCCACCCGCCCTGTACACCGGTCCGCACACGCGCCGCGACGGCCAACGTGTAGCGGACCCAGTCTTTCCCT
GAAAACGTTCGGAGCACACC

Downstream 100 bases:

>100_bases
GCGCGAAGGGTTCCGGGGGTCCGGCGGGCATTCCGCCGGACCCGGCAATCTCCTCGCCAGCTATCTCTTTCGCATTACCG
CTTTCGCTGTATCCTGATCC

Product: flavodoxin FldA

Products: NA

Alternate protein names: FldB [H]

Number of amino acids: Translated: 178; Mature: 177

Protein sequence:

>178_residues
MAKIGIFFGSDTGRTRKVAKYIAKKLGEVAADPVNVNKATVEDFLAYDALIIGSPTLGDGQLPGLESGAQNESWAEFLPQ
LDGADMSGKVVAIYGLGDQDKYANEFCDAIGLLYDAVTTCGATVVGPWPTEGYSFKSSQAVIDGQFCGLALDNDNQAGLT
DERVDTWLEQVKPALLGA

Sequences:

>Translated_178_residues
MAKIGIFFGSDTGRTRKVAKYIAKKLGEVAADPVNVNKATVEDFLAYDALIIGSPTLGDGQLPGLESGAQNESWAEFLPQ
LDGADMSGKVVAIYGLGDQDKYANEFCDAIGLLYDAVTTCGATVVGPWPTEGYSFKSSQAVIDGQFCGLALDNDNQAGLT
DERVDTWLEQVKPALLGA
>Mature_177_residues
AKIGIFFGSDTGRTRKVAKYIAKKLGEVAADPVNVNKATVEDFLAYDALIIGSPTLGDGQLPGLESGAQNESWAEFLPQL
DGADMSGKVVAIYGLGDQDKYANEFCDAIGLLYDAVTTCGATVVGPWPTEGYSFKSSQAVIDGQFCGLALDNDNQAGLTD
ERVDTWLEQVKPALLGA

Specific function: Low-potential electron donor to a number of redox enzymes. NifF is the electron donor to nitrogenase [H]

COG id: COG0716

COG function: function code C; Flavodoxins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Escherichia coli, GI1786900, Length=176, Percent_Identity=46.5909090909091, Blast_Score=155, Evalue=1e-39,
Organism=Escherichia coli, GI1789262, Length=171, Percent_Identity=42.1052631578947, Blast_Score=127, Evalue=4e-31,

Paralogues:

None

Copy number: 242 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001226
- InterPro:   IPR010086 [H]

Pfam domain/function: PF00258 Flavodoxin_1 [H]

EC number: NA

Molecular weight: Translated: 18817; Mature: 18686

Theoretical pI: Translated: 4.03; Mature: 4.03

Prosite motif: PS00201 FLAVODOXIN ; PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIGIFFGSDTGRTRKVAKYIAKKLGEVAADPVNVNKATVEDFLAYDALIIGSPTLGDG
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEECCCCCCCC
QLPGLESGAQNESWAEFLPQLDGADMSGKVVAIYGLGDQDKYANEFCDAIGLLYDAVTTC
CCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHC
GATVVGPWPTEGYSFKSSQAVIDGQFCGLALDNDNQAGLTDERVDTWLEQVKPALLGA
CCEEECCCCCCCCCCCCCCEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKIGIFFGSDTGRTRKVAKYIAKKLGEVAADPVNVNKATVEDFLAYDALIIGSPTLGDG
CEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEECCCCCCCC
QLPGLESGAQNESWAEFLPQLDGADMSGKVVAIYGLGDQDKYANEFCDAIGLLYDAVTTC
CCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHC
GATVVGPWPTEGYSFKSSQAVIDGQFCGLALDNDNQAGLTDERVDTWLEQVKPALLGA
CCEEECCCCCCCCCCCCCCEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1859358 [H]