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The map label for this gene is folE [H]

Identifier: 119898424

GI number: 119898424

Start: 2338181

End: 2338852

Strand: Direct

Name: folE [H]

Synonym: azo2133

Alternate gene names: 119898424

Gene position: 2338181-2338852 (Clockwise)

Preceding gene: 119898422

Following gene: 119898429

Centisome position: 53.43

GC content: 68.45

Gene sequence:

>672_bases
ATGTCCTCCACCCATTTCCAACATTCCGACAGCGCCGGGGGCGACCACGATCACGGCCATACCCCTCATCCGCGGACGGT
CGGCATCCCCCGCTCCGGCAACTTTGATCCCGTGGCGTTCGAACGTGCGGTCGGCGACCTGCTTCGGGCGTGCGGCGTCG
AGCCCGACACCACGCACACCGGCCGGACGGCGCAGAGGGTGCGCGAACTGTGGCAGAAGCGTCTGCTCGGAGGCTATGAA
CTCGACCCCGCTGCGGCGCTCGGCGAGGGCTTCGAGGATCCGCGCCGCGACATGGTCGTGGTCCGCGGCATCGCCATTCA
CGGCGTCTGTCCGCATCATCTCGTTCCGTTCCGCGGCCTCGCCCACGTCGCCTACGTGCCGGGTGGGCGCCTGCACGGCT
TCGGCCGCATCGCCCGGCTGGTGGACGCCATCGGGCACCGTTTCACCTACCAGGAATGGATGACCCGCGACATTGCGGAT
GCACTGATGCACCACGGCAGTGCATCCGGCGCCGCATGCGTGATTGAAGCGGAGCAACTGTGCCTGCTGCTCGGGGAGGA
CCGGCGCGGCGACGAGCGCGTGGTGACGCAGGCCTTCGCCGGCGATTTCGAACGCTCGGATCAGTTGCGCAACGAGTTCC
TGCGCGCAATCGCCCCCAACCGGGCGCTCTGA

Upstream 100 bases:

>100_bases
TCGACAAGCAGGACGCGTGCCGGTTTTGGTGCAGCGCAACATTCCGGCCCCGCCTGGGCTTCAAGCGTATGATCCGCCAA
CCTTCGCAGGAAATCCGACA

Downstream 100 bases:

>100_bases
GCGCTACTGGACGATGTGGAAGCGCGGGCGATGCGCGGCGAGATCCAGCAGCACCACTTCCCGTCCGCGCAGCGTCACTC
CGCCGCGGAAGCGGTCATAG

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MSSTHFQHSDSAGGDHDHGHTPHPRTVGIPRSGNFDPVAFERAVGDLLRACGVEPDTTHTGRTAQRVRELWQKRLLGGYE
LDPAAALGEGFEDPRRDMVVVRGIAIHGVCPHHLVPFRGLAHVAYVPGGRLHGFGRIARLVDAIGHRFTYQEWMTRDIAD
ALMHHGSASGAACVIEAEQLCLLLGEDRRGDERVVTQAFAGDFERSDQLRNEFLRAIAPNRAL

Sequences:

>Translated_223_residues
MSSTHFQHSDSAGGDHDHGHTPHPRTVGIPRSGNFDPVAFERAVGDLLRACGVEPDTTHTGRTAQRVRELWQKRLLGGYE
LDPAAALGEGFEDPRRDMVVVRGIAIHGVCPHHLVPFRGLAHVAYVPGGRLHGFGRIARLVDAIGHRFTYQEWMTRDIAD
ALMHHGSASGAACVIEAEQLCLLLGEDRRGDERVVTQAFAGDFERSDQLRNEFLRAIAPNRAL
>Mature_222_residues
SSTHFQHSDSAGGDHDHGHTPHPRTVGIPRSGNFDPVAFERAVGDLLRACGVEPDTTHTGRTAQRVRELWQKRLLGGYEL
DPAAALGEGFEDPRRDMVVVRGIAIHGVCPHHLVPFRGLAHVAYVPGGRLHGFGRIARLVDAIGHRFTYQEWMTRDIADA
LMHHGSASGAACVIEAEQLCLLLGEDRRGDERVVTQAFAGDFERSDQLRNEFLRAIAPNRAL

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=176, Percent_Identity=28.9772727272727, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI4503949, Length=176, Percent_Identity=28.9772727272727, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI66932970, Length=138, Percent_Identity=29.7101449275362, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI66932972, Length=136, Percent_Identity=30.1470588235294, Blast_Score=72, Evalue=3e-13,
Organism=Escherichia coli, GI1788476, Length=157, Percent_Identity=30.5732484076433, Blast_Score=69, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17560486, Length=214, Percent_Identity=28.0373831775701, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321706, Length=124, Percent_Identity=37.0967741935484, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24656791, Length=172, Percent_Identity=29.6511627906977, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24656796, Length=172, Percent_Identity=29.6511627906977, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24656782, Length=172, Percent_Identity=29.6511627906977, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 24532; Mature: 24401

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTHFQHSDSAGGDHDHGHTPHPRTVGIPRSGNFDPVAFERAVGDLLRACGVEPDTTHT
CCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
GRTAQRVRELWQKRLLGGYELDPAAALGEGFEDPRRDMVVVRGIAIHGVCPHHLVPFRGL
CHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCEEEEECEEEECCCCHHHCCHHHH
AHVAYVPGGRLHGFGRIARLVDAIGHRFTYQEWMTRDIADALMHHGSASGAACVIEAEQL
HHEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHH
CLLLGEDRRGDERVVTQAFAGDFERSDQLRNEFLRAIAPNRAL
HHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SSTHFQHSDSAGGDHDHGHTPHPRTVGIPRSGNFDPVAFERAVGDLLRACGVEPDTTHT
CCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
GRTAQRVRELWQKRLLGGYELDPAAALGEGFEDPRRDMVVVRGIAIHGVCPHHLVPFRGL
CHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCEEEEECEEEECCCCHHHCCHHHH
AHVAYVPGGRLHGFGRIARLVDAIGHRFTYQEWMTRDIADALMHHGSASGAACVIEAEQL
HHEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHH
CLLLGEDRRGDERVVTQAFAGDFERSDQLRNEFLRAIAPNRAL
HHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]