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The map label for this gene is tesA2 [H]

Identifier: 119898414

GI number: 119898414

Start: 2329257

End: 2329883

Strand: Direct

Name: tesA2 [H]

Synonym: azo2123

Alternate gene names: 119898414

Gene position: 2329257-2329883 (Clockwise)

Preceding gene: 119898413

Following gene: 119898415

Centisome position: 53.23

GC content: 70.97

Gene sequence:

>627_bases
ATGCGGCGCTGGCTGCTGTTCGCCCTCACGCTCGCGTTGGCGGCGTGCGGCAGCGACCGTCCCAAGTACGCCCCGCTGCC
ACCGGGCAGCGTGGTGCTCGCGTTCGGCGACAGCGTCACCTGGGGCACCGGCGCCGCCCGCGGCGAGGACTACCCTACCC
GGCTGGCCGCGCTCAGCGGCTGGAGGATCGAGAACGCCGGCGTTCCCGGCGACCTCGCCGAAACCGCGCGCAATCGCATT
GACGAAGCCCTGGAGGAGCACGCGCCCCGCCTCGTGCTGGTCGAACTCGGCGGCAACGACTTCCTGCGTCGACGTCCGGA
ACAGGACGTCGCCGAGGATCTGCGCGCCATCCTTCGCAGCGTGAAGGCGGCAGGTGCCCAGCCGGTGCTGGTGGCGGTGC
CGGCCTTTTCGCCTCTTGCCGCGGCCACCAGCGGTTTGAAGGATGCCCTGATCTACCGCCGCCTGGCCGACGAAGAGGCC
GTACCGCTGGTCGGAGCCGTGTTCGCCGACGTCCTGTCCGACACGAAGCTGCGCGCCGACCCCATCCACCCCAACGCCCA
GGGTTATCACCGTCTGGCCGAGGGCATCGCCGAGTCCCTGCGCGACCACGGCCTGCTCGCAAACTGA

Upstream 100 bases:

>100_bases
CCCACGCCAAGACGGAGCGCGCGCCCTACCCGCCCAAGTCCGGCGAAAAGCCGCGCCCGGCCGGCGGTGACAAGCCCTGG
AAGCGCAAGGACAAACGCTG

Downstream 100 bases:

>100_bases
ACCATGTCCGACGTGCCAACCCCGCGGGAACTTCCGCCGAGCCTGGATGGCCAGGTGCCAACGACATGAACCCACCGTTC
GCCATCCTGACGCCCGACCT

Product: putative acyl-CoA thioesterase I

Products: NA

Alternate protein names: Lecithinase B; Lysophospholipase L1; Protease I [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MRRWLLFALTLALAACGSDRPKYAPLPPGSVVLAFGDSVTWGTGAARGEDYPTRLAALSGWRIENAGVPGDLAETARNRI
DEALEEHAPRLVLVELGGNDFLRRRPEQDVAEDLRAILRSVKAAGAQPVLVAVPAFSPLAAATSGLKDALIYRRLADEEA
VPLVGAVFADVLSDTKLRADPIHPNAQGYHRLAEGIAESLRDHGLLAN

Sequences:

>Translated_208_residues
MRRWLLFALTLALAACGSDRPKYAPLPPGSVVLAFGDSVTWGTGAARGEDYPTRLAALSGWRIENAGVPGDLAETARNRI
DEALEEHAPRLVLVELGGNDFLRRRPEQDVAEDLRAILRSVKAAGAQPVLVAVPAFSPLAAATSGLKDALIYRRLADEEA
VPLVGAVFADVLSDTKLRADPIHPNAQGYHRLAEGIAESLRDHGLLAN
>Mature_208_residues
MRRWLLFALTLALAACGSDRPKYAPLPPGSVVLAFGDSVTWGTGAARGEDYPTRLAALSGWRIENAGVPGDLAETARNRI
DEALEEHAPRLVLVELGGNDFLRRRPEQDVAEDLRAILRSVKAAGAQPVLVAVPAFSPLAAATSGLKDALIYRRLADEEA
VPLVGAVFADVLSDTKLRADPIHPNAQGYHRLAEGIAESLRDHGLLAN

Specific function: Hydrolyzes only long chain acyl thioesters (C12-C18). Specificity similar to chymotrypsin [H]

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786702, Length=207, Percent_Identity=31.8840579710145, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087
- InterPro:   IPR008265 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: =3.1.1.5 [H]

Molecular weight: Translated: 22230; Mature: 22230

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00687 ALDEHYDE_DEHYDR_GLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRWLLFALTLALAACGSDRPKYAPLPPGSVVLAFGDSVTWGTGAARGEDYPTRLAALSG
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHCC
WRIENAGVPGDLAETARNRIDEALEEHAPRLVLVELGGNDFLRRRPEQDVAEDLRAILRS
CEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHCCCHHHHHHHHHHHHHH
VKAAGAQPVLVAVPAFSPLAAATSGLKDALIYRRLADEEAVPLVGAVFADVLSDTKLRAD
HHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCC
PIHPNAQGYHRLAEGIAESLRDHGLLAN
CCCCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRRWLLFALTLALAACGSDRPKYAPLPPGSVVLAFGDSVTWGTGAARGEDYPTRLAALSG
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHCC
WRIENAGVPGDLAETARNRIDEALEEHAPRLVLVELGGNDFLRRRPEQDVAEDLRAILRS
CEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHCCCHHHHHHHHHHHHHH
VKAAGAQPVLVAVPAFSPLAAATSGLKDALIYRRLADEEAVPLVGAVFADVLSDTKLRAD
HHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCC
PIHPNAQGYHRLAEGIAESLRDHGLLAN
CCCCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]