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The map label for this gene is wspR1 [H]

Identifier: 119898350

GI number: 119898350

Start: 2258225

End: 2259202

Strand: Reverse

Name: wspR1 [H]

Synonym: azo2059

Alternate gene names: 119898350

Gene position: 2259202-2258225 (Counterclockwise)

Preceding gene: 119898352

Following gene: 119898349

Centisome position: 51.63

GC content: 64.72

Gene sequence:

>978_bases
ATGAAAGTCCTGGTCATAGAAGACACCGTCACCAGCGCCACGCTGGTCTGCCACCAGCTCACCAAAATGGGCCTGACGGC
GCTACACGCCCGCGACGGCGAATCCGGCATCGAAACCTTCCGCAGGGAACGGCCGGATCTCATCCTGCTCGACATCATCA
TGCCCGGCATGGACGGATTCGAAGTCGCACGCCGGATTCGCGAAATCGAGTCCGAAGGTGAATGGACGCCGATCATTTTC
CTCACCGCGCGCACGTCCGACGAGGACCTCAACCGCGGGATCGAGGTCGGCGGCGACGACTATCTGGCCAAGCCGGTATC
CGAAGTGGTGCTGAAGGCCAAGGTGCGGGCAATGCAGCGCATTGCGCAGATGCGTTACTCGCTGCTCGTGGTGACGCGCA
AGCTGGACGAGGCTAACCGCGAATTGACCCGGCTGTCGTCGGTGGACGGACTGACCGGCATTCCCAATCGCCGCCGTTTC
GACGAAACGCTGCTGCGGGAGTGGCGACGCGCGGCGCGCACCGGGCACCCGATCGCACTGCTCGTGGCCGACGTCGATTC
GTTCAAGCAGTTCAACGACGGCTACGGCCACCAAGTGGGCGACGAGTGCCTGAAAGCCGTTGCACGCACGCTGGAACAGC
GGCTGCGCCGGCCGACCGATCTGGTTGCGCGCTACGGCGGCGAGGAATTCGCGGCAATCCTTCCCGAAACCAGCGTCGAA
GGCGCGGCCGCCGTTGCCGAAACCATGCGCGCCGGTGTCGAAAGCCTGTGCATCACGCACCGCTTCTCCACCTCGGGCCC
GGTGGTGTCGATCAGTATCGGCGTCGCCGCCATCGTGCCGGCGCGAGGCGATGAGAACGGATTCGTCGCGCTGTTGAAGT
CGGCCGACGACGCGCTGTACCGCGCCAAGGCGGCGGGGCGCAACCGTGTGGTCACGCATTCGGTGGACACGAAAGGAATG
GAAGCGCGCACAATCTGA

Upstream 100 bases:

>100_bases
CGTGCGGAAATACCGAAAACACGACTAAAGGCGTAGCCGTGGGGTATTCCCTTGGTAGGATGGCGCATCCACGTAGTACC
GATTCTTCGTGCTCGACATC

Downstream 100 bases:

>100_bases
GCATGCAGCGCTACAGCAAGAGAATCGTCGTCGTCGACGACAATGAAGTGATCCGGATGGCCCTGCGCGCCCTGTTGCGC
CAGGCCGGTTTCAATGTGGT

Product: REC/GGDEF-domain-containing protein

Products: NA

Alternate protein names: Bacteriophytochrome cph2 [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MKVLVIEDTVTSATLVCHQLTKMGLTALHARDGESGIETFRRERPDLILLDIIMPGMDGFEVARRIREIESEGEWTPIIF
LTARTSDEDLNRGIEVGGDDYLAKPVSEVVLKAKVRAMQRIAQMRYSLLVVTRKLDEANRELTRLSSVDGLTGIPNRRRF
DETLLREWRRAARTGHPIALLVADVDSFKQFNDGYGHQVGDECLKAVARTLEQRLRRPTDLVARYGGEEFAAILPETSVE
GAAAVAETMRAGVESLCITHRFSTSGPVVSISIGVAAIVPARGDENGFVALLKSADDALYRAKAAGRNRVVTHSVDTKGM
EARTI

Sequences:

>Translated_325_residues
MKVLVIEDTVTSATLVCHQLTKMGLTALHARDGESGIETFRRERPDLILLDIIMPGMDGFEVARRIREIESEGEWTPIIF
LTARTSDEDLNRGIEVGGDDYLAKPVSEVVLKAKVRAMQRIAQMRYSLLVVTRKLDEANRELTRLSSVDGLTGIPNRRRF
DETLLREWRRAARTGHPIALLVADVDSFKQFNDGYGHQVGDECLKAVARTLEQRLRRPTDLVARYGGEEFAAILPETSVE
GAAAVAETMRAGVESLCITHRFSTSGPVVSISIGVAAIVPARGDENGFVALLKSADDALYRAKAAGRNRVVTHSVDTKGM
EARTI
>Mature_325_residues
MKVLVIEDTVTSATLVCHQLTKMGLTALHARDGESGIETFRRERPDLILLDIIMPGMDGFEVARRIREIESEGEWTPIIF
LTARTSDEDLNRGIEVGGDDYLAKPVSEVVLKAKVRAMQRIAQMRYSLLVVTRKLDEANRELTRLSSVDGLTGIPNRRRF
DETLLREWRRAARTGHPIALLVADVDSFKQFNDGYGHQVGDECLKAVARTLEQRLRRPTDLVARYGGEEFAAILPETSVE
GAAAVAETMRAGVESLCITHRFSTSGPVVSISIGVAAIVPARGDENGFVALLKSADDALYRAKAAGRNRVVTHSVDTKGM
EARTI

Specific function: Photoreceptor which exists in two forms that are reversibly interconvertible by light:the R form that absorbs maximally in the red region of the spectrum and the FR form that absorbs maximally in the far-red region [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Integral Membrane Protein [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 GGDEF domains [H]

Homologues:

Organism=Escherichia coli, GI1786584, Length=176, Percent_Identity=39.7727272727273, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI87081881, Length=188, Percent_Identity=40.9574468085106, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI145693134, Length=192, Percent_Identity=35.9375, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787262, Length=171, Percent_Identity=37.4269005847953, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1786599, Length=124, Percent_Identity=37.9032258064516, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788085, Length=192, Percent_Identity=32.2916666666667, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87082012, Length=179, Percent_Identity=34.0782122905028, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787816, Length=170, Percent_Identity=34.7058823529412, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787802, Length=197, Percent_Identity=33.502538071066, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI87082007, Length=138, Percent_Identity=40.5797101449275, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788381, Length=178, Percent_Identity=33.7078651685393, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1786784, Length=120, Percent_Identity=35, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789402, Length=120, Percent_Identity=33.3333333333333, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789809, Length=119, Percent_Identity=34.453781512605, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787541, Length=172, Percent_Identity=29.6511627906977, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790860, Length=118, Percent_Identity=32.2033898305085, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788394, Length=119, Percent_Identity=31.9327731092437, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI145693157, Length=112, Percent_Identity=33.9285714285714, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87081977, Length=175, Percent_Identity=29.7142857142857, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI87081974, Length=182, Percent_Identity=26.3736263736264, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1787056, Length=164, Percent_Identity=32.9268292682927, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI145693140, Length=109, Percent_Identity=33.0275229357798, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787487, Length=108, Percent_Identity=29.6296296296296, Blast_Score=62, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6322000, Length=107, Percent_Identity=37.3831775700935, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR003018
- InterPro:   IPR016132
- InterPro:   IPR001294
- InterPro:   IPR013515 [H]

Pfam domain/function: PF00563 EAL; PF01590 GAF; PF00990 GGDEF; PF00360 Phytochrome [H]

EC number: NA

Molecular weight: Translated: 35813; Mature: 35813

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLVIEDTVTSATLVCHQLTKMGLTALHARDGESGIETFRRERPDLILLDIIMPGMDGF
CEEEEEECCHHHHHHHHHHHHHHCHHHEECCCCHHHHHHHHHCCCCEEEEEECCCCCCHH
EVARRIREIESEGEWTPIIFLTARTSDEDLNRGIEVGGDDYLAKPVSEVVLKAKVRAMQR
HHHHHHHHHHCCCCEEEEEEEEECCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
IAQMRYSLLVVTRKLDEANRELTRLSSVDGLTGIPNRRRFDETLLREWRRAARTGHPIAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEE
LVADVDSFKQFNDGYGHQVGDECLKAVARTLEQRLRRPTDLVARYGGEEFAAILPETSVE
EEECHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHCCCCCCC
GAAAVAETMRAGVESLCITHRFSTSGPVVSISIGVAAIVPARGDENGFVALLKSADDALY
HHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEECEEEEEECCCCCCCEEEEEECCCHHHH
RAKAAGRNRVVTHSVDTKGMEARTI
HHHHCCCCCEEEECCCCCCCCCCCC
>Mature Secondary Structure
MKVLVIEDTVTSATLVCHQLTKMGLTALHARDGESGIETFRRERPDLILLDIIMPGMDGF
CEEEEEECCHHHHHHHHHHHHHHCHHHEECCCCHHHHHHHHHCCCCEEEEEECCCCCCHH
EVARRIREIESEGEWTPIIFLTARTSDEDLNRGIEVGGDDYLAKPVSEVVLKAKVRAMQR
HHHHHHHHHHCCCCEEEEEEEEECCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
IAQMRYSLLVVTRKLDEANRELTRLSSVDGLTGIPNRRRFDETLLREWRRAARTGHPIAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEE
LVADVDSFKQFNDGYGHQVGDECLKAVARTLEQRLRRPTDLVARYGGEEFAAILPETSVE
EEECHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHCCCCCCC
GAAAVAETMRAGVESLCITHRFSTSGPVVSISIGVAAIVPARGDENGFVALLKSADDALY
HHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEECEEEEEECCCCCCCEEEEEECCCHHHH
RAKAAGRNRVVTHSVDTKGMEARTI
HHHHCCCCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231; 10978170; 11063585 [H]