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The map label for this gene is gloB2 [H]

Identifier: 119898348

GI number: 119898348

Start: 2257071

End: 2257832

Strand: Direct

Name: gloB2 [H]

Synonym: azo2057

Alternate gene names: 119898348

Gene position: 2257071-2257832 (Clockwise)

Preceding gene: 119898344

Following gene: 119898351

Centisome position: 51.58

GC content: 63.12

Gene sequence:

>762_bases
ATGCAGATTATCCCTCTTCCGGCCTTCCGCGATAACTACATTTGGGTCCTGCACGACGATCATCGTGCTGTGGTCGTCGA
TCCAGGCGACGCTGCGCCAGTGCTCGGTTTTCTTGCCGAGCGCGGCCTGGCGCTGGAGGCCGTGCTGGTTACCCACCACC
ATCCGGACCACGTAGACGGGATTGCCGGAATCGTTTCCCGCCATCCGGCGCCGGTGTACGGGCCGGCGGCAGAAGCGATC
GCCGGCGTGACCGTCCGGTTGCGCGAAGGTGACACCGTGCTCCTGCCCGGGCTTCGAATGCAGCTGGGGGTGCTGGACGT
ACCCGGTCACACCGCAGGCCATATTGCCTACCATGGCAAGGGTTATCTGTTTTGCGGGGATACGCTGTTCTCCGCCGGAT
GTGGTCGGCTGTTCGAGGGCACCGCCGCGCAGATGGCTGCATCGCTCGACAAACTCGCAGCGCTGCCGGACGACACTGTC
GTGTATTGCACGCACGAATACACCTTGTCCAACCTTGCTTTTGCCGCGGCGGCAGAACCGGTGAATATCGAGCGCGACAA
CTACCGAAAAGCGTGTGAGGCGCTGAGGCAGCAGGGGCTTCCGACGCTGCCGACTTCGATCGGACGCGAAAAAGCGATCA
ACCCCTTTCTCCGTACCCGGCAGCCCGGTGTTGTTGCAGGCGTTGCCGCCCATACCGGTACGACGCCAGACACGCCGCTG
GCGTGCTTCGCCGCGTTGCGGGAATGGAAGAACGTGTTCTGA

Upstream 100 bases:

>100_bases
GGCCTGCTCCCACTCGATCAGATAACGCCCCTGCGGGGTGTCCAGCCAGTCCGAGAGACTGAGGATTGACATGCGCGGAT
CGGACTTCAGAATATCGAGC

Downstream 100 bases:

>100_bases
AGGCTATTGGGTCGCGGTCAGGCCGAGCGTCCGCTCAACCAGCTCGATGATACGTGCGGCATTGAAGGGTTTGACGACGA
TGCCGGCCGCACCCTTGCGG

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MQIIPLPAFRDNYIWVLHDDHRAVVVDPGDAAPVLGFLAERGLALEAVLVTHHHPDHVDGIAGIVSRHPAPVYGPAAEAI
AGVTVRLREGDTVLLPGLRMQLGVLDVPGHTAGHIAYHGKGYLFCGDTLFSAGCGRLFEGTAAQMAASLDKLAALPDDTV
VYCTHEYTLSNLAFAAAAEPVNIERDNYRKACEALRQQGLPTLPTSIGREKAINPFLRTRQPGVVAGVAAHTGTTPDTPL
ACFAALREWKNVF

Sequences:

>Translated_253_residues
MQIIPLPAFRDNYIWVLHDDHRAVVVDPGDAAPVLGFLAERGLALEAVLVTHHHPDHVDGIAGIVSRHPAPVYGPAAEAI
AGVTVRLREGDTVLLPGLRMQLGVLDVPGHTAGHIAYHGKGYLFCGDTLFSAGCGRLFEGTAAQMAASLDKLAALPDDTV
VYCTHEYTLSNLAFAAAAEPVNIERDNYRKACEALRQQGLPTLPTSIGREKAINPFLRTRQPGVVAGVAAHTGTTPDTPL
ACFAALREWKNVF
>Mature_253_residues
MQIIPLPAFRDNYIWVLHDDHRAVVVDPGDAAPVLGFLAERGLALEAVLVTHHHPDHVDGIAGIVSRHPAPVYGPAAEAI
AGVTVRLREGDTVLLPGLRMQLGVLDVPGHTAGHIAYHGKGYLFCGDTLFSAGCGRLFEGTAAQMAASLDKLAALPDDTV
VYCTHEYTLSNLAFAAAAEPVNIERDNYRKACEALRQQGLPTLPTSIGREKAINPFLRTRQPGVVAGVAAHTGTTPDTPL
ACFAALREWKNVF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI116642887, Length=229, Percent_Identity=38.4279475982533, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI21703352, Length=229, Percent_Identity=38.4279475982533, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI94538320, Length=264, Percent_Identity=36.3636363636364, Blast_Score=142, Evalue=2e-34,
Organism=Homo sapiens, GI94538322, Length=264, Percent_Identity=36.3636363636364, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI14150041, Length=233, Percent_Identity=36.0515021459227, Blast_Score=138, Evalue=5e-33,
Organism=Homo sapiens, GI46361987, Length=191, Percent_Identity=38.2198952879581, Blast_Score=120, Evalue=1e-27,
Organism=Escherichia coli, GI1786406, Length=255, Percent_Identity=46.6666666666667, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1787158, Length=206, Percent_Identity=32.0388349514563, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17536925, Length=256, Percent_Identity=34.375, Blast_Score=137, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17538952, Length=174, Percent_Identity=29.3103448275862, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320478, Length=239, Percent_Identity=32.2175732217573, Blast_Score=91, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324614, Length=263, Percent_Identity=28.8973384030418, Blast_Score=83, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21356335, Length=271, Percent_Identity=39.4833948339483, Blast_Score=173, Evalue=8e-44,
Organism=Drosophila melanogaster, GI24667711, Length=271, Percent_Identity=39.4833948339483, Blast_Score=173, Evalue=9e-44,
Organism=Drosophila melanogaster, GI24667703, Length=271, Percent_Identity=39.4833948339483, Blast_Score=172, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 27036; Mature: 27036

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIIPLPAFRDNYIWVLHDDHRAVVVDPGDAAPVLGFLAERGLALEAVLVTHHHPDHVDG
CEEECCCCCCCCEEEEEECCCEEEEECCCCCHHHHHHHHHCCCEEEEEEEECCCCCHHHH
IAGIVSRHPAPVYGPAAEAIAGVTVRLREGDTVLLPGLRMQLGVLDVPGHTAGHIAYHGK
HHHHHHCCCCCCCCCHHHHHCCEEEEEECCCEEEECCCEEEEEEEECCCCCCCEEEECCC
GYLFCGDTLFSAGCGRLFEGTAAQMAASLDKLAALPDDTVVYCTHEYTLSNLAFAAAAEP
EEEEECHHHHHHCCCHHHCCHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCC
VNIERDNYRKACEALRQQGLPTLPTSIGREKAINPFLRTRQPGVVAGVAAHTGTTPDTPL
CCCCHHHHHHHHHHHHHCCCCCCCHHCCHHHHCCHHHHCCCCCEEEEEEECCCCCCCCHH
ACFAALREWKNVF
HHHHHHHHHHHCC
>Mature Secondary Structure
MQIIPLPAFRDNYIWVLHDDHRAVVVDPGDAAPVLGFLAERGLALEAVLVTHHHPDHVDG
CEEECCCCCCCCEEEEEECCCEEEEECCCCCHHHHHHHHHCCCEEEEEEEECCCCCHHHH
IAGIVSRHPAPVYGPAAEAIAGVTVRLREGDTVLLPGLRMQLGVLDVPGHTAGHIAYHGK
HHHHHHCCCCCCCCCHHHHHCCEEEEEECCCEEEECCCEEEEEEEECCCCCCCEEEECCC
GYLFCGDTLFSAGCGRLFEGTAAQMAASLDKLAALPDDTVVYCTHEYTLSNLAFAAAAEP
EEEEECHHHHHHCCCHHHCCHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCC
VNIERDNYRKACEALRQQGLPTLPTSIGREKAINPFLRTRQPGVVAGVAAHTGTTPDTPL
CCCCHHHHHHHHHHHHHCCCCCCCHHCCHHHHCCHHHHCCCCCEEEEEEECCCCCCCCHH
ACFAALREWKNVF
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA