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The map label for this gene is carBa2 [C]

Identifier: 119898275

GI number: 119898275

Start: 2183143

End: 2183625

Strand: Direct

Name: carBa2 [C]

Synonym: azo1984

Alternate gene names: 119898275

Gene position: 2183143-2183625 (Clockwise)

Preceding gene: 119898274

Following gene: 119898276

Centisome position: 49.89

GC content: 62.94

Gene sequence:

>483_bases
ATGACCGCCATCACCGAAAAGACCCTCACCGACTTCGTCTATGCCGAAGCCCGCATGCTCGACGAGCAGCGCTTCAACGA
CTGGCTCGCGCTCTTCACCGACGACGCCTACTACTGGATGCCGCTCGCGCACGACCAGCAGGACCCCAAGCTCCACACCT
CGTTGCTGTACGAAGACACGCTGCTGCTGCGCGTGCGCATCGAGCGCCTGAACGGCCAGCGCACCTTCTCGCAGCAGCCC
AAGAGCCGCTGCCATCACCTGCTGCAGGCCCCGAGCATCGAAGCCTTCGACGCCGACAAGGGCGAATACACCGTGCGCAC
CGCCTTTCACTACACCGAAACCCGCCAGGACAAGCAGACGCTGTACGCCGGCTGGGCCACCCATCAACTCGTCGCGGTGG
ATGGCGTGCTCAAGATCCGCCAGAAGCGCGTCGATCTGGTCAATTGCGATGCCGCCTTCGGCAACATCCAGCTCTTCATG
TAA

Upstream 100 bases:

>100_bases
ACCCTCCCCTTCAAGGGGAGGGAGTTTGGCCACCGGGCCTGCCCCGCCTGAGCGCGCGCACCGACGCACGGAGCTGCGAC
ACCCGCAAAAGGACAATGCC

Downstream 100 bases:

>100_bases
GGCGGAGAACATCTTGGCAAACACTGTTCATCAGGCCTTCTCGCTCGCCGCGGTGCGTTGGGCGAAACAGCCCTTCCTCT
GCATCCTGCCGGAGACCGCG

Product: putative dioxygenase, hydroxylase small subunit

Products: NAD; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol [C]

Alternate protein names: Aromatic 12-Dioxygenase Beta Subunit; Ring Hydroxylating Subunit Beta Family; Dioxygenase Hydroxylase Small Subunit; Small Subunit Of Terminal Oxygenase Component; Angular Dioxygenase Small Subunit; Dioxygenase Beta Subunit; P-Cumate Dioxygenase Small Subunit; Ring-Hydroxylating Dioxygenase Small Subunit; Small Subunit Of Phenylpropionate Dioxygenase; Dioxygenase Component; Aromatic-Ring-Hydroxylating Dioxygenase Subunit Beta; Aromatic Hydrocarbon Dioxygenase Small Subunit; Dioxygenase Component Beta Subunit; Aromatic 1 2-Dioxygenase Beta Subunit

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MTAITEKTLTDFVYAEARMLDEQRFNDWLALFTDDAYYWMPLAHDQQDPKLHTSLLYEDTLLLRVRIERLNGQRTFSQQP
KSRCHHLLQAPSIEAFDADKGEYTVRTAFHYTETRQDKQTLYAGWATHQLVAVDGVLKIRQKRVDLVNCDAAFGNIQLFM

Sequences:

>Translated_160_residues
MTAITEKTLTDFVYAEARMLDEQRFNDWLALFTDDAYYWMPLAHDQQDPKLHTSLLYEDTLLLRVRIERLNGQRTFSQQP
KSRCHHLLQAPSIEAFDADKGEYTVRTAFHYTETRQDKQTLYAGWATHQLVAVDGVLKIRQKRVDLVNCDAAFGNIQLFM
>Mature_159_residues
TAITEKTLTDFVYAEARMLDEQRFNDWLALFTDDAYYWMPLAHDQQDPKLHTSLLYEDTLLLRVRIERLNGQRTFSQQPK
SRCHHLLQAPSIEAFDADKGEYTVRTAFHYTETRQDKQTLYAGWATHQLVAVDGVLKIRQKRVDLVNCDAAFGNIQLFM

Specific function: Part Of The Multicomponent 3-Phenylpropionate Dioxygenase. Converts 3-Phenylpropionic Acid (Pp) Into Cis-3-(3- Carboxyethyl)-3,5-Cyclohexadiene-1,2-Diol (Pp-Dihydrodiol). [C]

COG id: COG5517

COG function: function code Q; Small subunit of phenylpropionate dioxygenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.14.1.- [C]

Molecular weight: Translated: 18716; Mature: 18585

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAITEKTLTDFVYAEARMLDEQRFNDWLALFTDDAYYWMPLAHDQQDPKLHTSLLYEDT
CCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCCCCCHHHHHHHCCC
LLLRVRIERLNGQRTFSQQPKSRCHHLLQAPSIEAFDADKGEYTVRTAFHYTETRQDKQT
EEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCEEECCCCCEEEEEEEEECCCCCCHHH
LYAGWATHQLVAVDGVLKIRQKRVDLVNCDAAFGNIQLFM
EEECCCCCEEEEHHHHHHHHHHHCCEEECCCCCCEEEEEC
>Mature Secondary Structure 
TAITEKTLTDFVYAEARMLDEQRFNDWLALFTDDAYYWMPLAHDQQDPKLHTSLLYEDT
CCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCCCCCHHHHHHHCCC
LLLRVRIERLNGQRTFSQQPKSRCHHLLQAPSIEAFDADKGEYTVRTAFHYTETRQDKQT
EEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCEEECCCCCEEEEEEEEECCCCCCHHH
LYAGWATHQLVAVDGVLKIRQKRVDLVNCDAAFGNIQLFM
EEECCCCCEEEEHHHHHHHHHHHCCEEECCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; Proton; O2; 3-phenylpropionate [C]

Specific reaction: 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD 3-phenylpropionate + NADH + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA