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The map label for this gene is carBb2 [H]

Identifier: 119898274

GI number: 119898274

Start: 2181600

End: 2182922

Strand: Direct

Name: carBb2 [H]

Synonym: azo1983

Alternate gene names: 119898274

Gene position: 2181600-2182922 (Clockwise)

Preceding gene: 119898273

Following gene: 119898275

Centisome position: 49.85

GC content: 64.55

Gene sequence:

>1323_bases
ATGAGCACGACCAACACGTATCGCGGCAACCCGGACGCCATCCGCGCGCTGGTGCGCGACACCGAGGTGCACAAGGACAC
CTACATCGACCAGGAACTGTTCGAGCTGGAGATGGAGCATCTCTTCGCCAACACCTGGGTCTATGTCGGCCACGCCAGCC
AGGTGCCGAACAAGGGCGACTACTACACCACCACCGTCGGCACCGAGCCGGTGGTGATGGTGCGCCACACCGACGACAGC
ATCCGCGTGCTCTACAACCGCTGTCCGCACAAGGGCGTGAAGGTGGCGGGCGAGGTCTGTGGCAACACCGGCAAGTTCTT
CCGCTGCCCCTACCACGCCTGGACCTTCAAGACCGACGGCAGCCTGCTCGCGATTCCGCTGAAGAAGGGCTACGAGAACA
CCGGCTTCGAAAACTGCGAGGCCAGCCAGGGCATGGCGCCGGTGAAGAACGTCAAGGTCTATCGCGACTTCGTGTTCTGC
CGGCTGAACCCGGGCGGCCCCAGCTTCGAGGAATACTTCGGCGAATCGCTCTCCACCATCGACAACATGGTGGACCGCTC
GCCCGAGGGCAAGCTGGAAGTCGCCGGCGGGGTGCTGCGCTACATGCACAACTGCAACTGGAAGATGCTGGTCGATAACC
AGACCGACACCTGCCACCCGATGGTCGCCCACGAATCCTCGGCCGGCACCGCCGTGCGGGTGTGGAACGAGGCCCCGGAA
GGCACGCCCAAACCGATGGCGGTGGAGCTCTTCGCGCCCTTCATGTCGCCCTACGAATTCTTCGAAGGCATGGGCATCCG
CGTATGGGAAAACGGCCACGGCCACACCGGCGTGTCCAACTCGATCCACGCCGCCTACTCCGCCATCCCCGGCTACTGGG
AGCAGATGGTGGCCGCCTACGGCGAGGACCGCGCCCACCAGATCCTCGGCGACGTGCGCCACAACACGGTGTACTGGCCC
AACATCATGATCAAGGGCCCGATCCAGACCCTGCGCCTGTTCAAGCCGATCGCGGCGGACAAGACGCTGGTCGAGTCCTG
GACCTTCCGCCTCGTCGGCGCGCCGGACATGCTGCTGGAGCGCACCACCATGTACAACCGCCTGATCAACGCCCCCACCT
CGGTGGTCGGCCACGACGACCTAGAAATGTACGAGCGCGCCCAGGAAGGCCTGCACGCCCGCGGCCGCGACTGGATCAAC
GTCGGCCGCCTGTACGACCCCGCCGAGAAGGACCAGAAGAACGTGGTGACCAACGGCACCAACGAAATGCAGATGCGCGC
CCAGTTCCGCGCGTGGCTGAAGTACATGACGGAAAGCATGTGA

Upstream 100 bases:

>100_bases
TCCGACGCCGAAAAGGCCGGCGGCAAACTGATCCAGATCTGCATCTCGCGCGCGAAGACGCCGCGCCTGGTGCTCGATCT
CTGACCCAAGGGGGACAACG

Downstream 100 bases:

>100_bases
GTCGAGAAGCGTCGCCGTTGCACCACGCGTGTCACGGCCCGGTTCTAGTTCCTCCCCCTTCAAGGGGAAGGCTAGGAGGG
GGATGGGGTTGGGGCTGCGC

Product: putative dioxygenase hydroxylase large subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MSTTNTYRGNPDAIRALVRDTEVHKDTYIDQELFELEMEHLFANTWVYVGHASQVPNKGDYYTTTVGTEPVVMVRHTDDS
IRVLYNRCPHKGVKVAGEVCGNTGKFFRCPYHAWTFKTDGSLLAIPLKKGYENTGFENCEASQGMAPVKNVKVYRDFVFC
RLNPGGPSFEEYFGESLSTIDNMVDRSPEGKLEVAGGVLRYMHNCNWKMLVDNQTDTCHPMVAHESSAGTAVRVWNEAPE
GTPKPMAVELFAPFMSPYEFFEGMGIRVWENGHGHTGVSNSIHAAYSAIPGYWEQMVAAYGEDRAHQILGDVRHNTVYWP
NIMIKGPIQTLRLFKPIAADKTLVESWTFRLVGAPDMLLERTTMYNRLINAPTSVVGHDDLEMYERAQEGLHARGRDWIN
VGRLYDPAEKDQKNVVTNGTNEMQMRAQFRAWLKYMTESM

Sequences:

>Translated_440_residues
MSTTNTYRGNPDAIRALVRDTEVHKDTYIDQELFELEMEHLFANTWVYVGHASQVPNKGDYYTTTVGTEPVVMVRHTDDS
IRVLYNRCPHKGVKVAGEVCGNTGKFFRCPYHAWTFKTDGSLLAIPLKKGYENTGFENCEASQGMAPVKNVKVYRDFVFC
RLNPGGPSFEEYFGESLSTIDNMVDRSPEGKLEVAGGVLRYMHNCNWKMLVDNQTDTCHPMVAHESSAGTAVRVWNEAPE
GTPKPMAVELFAPFMSPYEFFEGMGIRVWENGHGHTGVSNSIHAAYSAIPGYWEQMVAAYGEDRAHQILGDVRHNTVYWP
NIMIKGPIQTLRLFKPIAADKTLVESWTFRLVGAPDMLLERTTMYNRLINAPTSVVGHDDLEMYERAQEGLHARGRDWIN
VGRLYDPAEKDQKNVVTNGTNEMQMRAQFRAWLKYMTESM
>Mature_439_residues
STTNTYRGNPDAIRALVRDTEVHKDTYIDQELFELEMEHLFANTWVYVGHASQVPNKGDYYTTTVGTEPVVMVRHTDDSI
RVLYNRCPHKGVKVAGEVCGNTGKFFRCPYHAWTFKTDGSLLAIPLKKGYENTGFENCEASQGMAPVKNVKVYRDFVFCR
LNPGGPSFEEYFGESLSTIDNMVDRSPEGKLEVAGGVLRYMHNCNWKMLVDNQTDTCHPMVAHESSAGTAVRVWNEAPEG
TPKPMAVELFAPFMSPYEFFEGMGIRVWENGHGHTGVSNSIHAAYSAIPGYWEQMVAAYGEDRAHQILGDVRHNTVYWPN
IMIKGPIQTLRLFKPIAADKTLVESWTFRLVGAPDMLLERTTMYNRLINAPTSVVGHDDLEMYERAQEGLHARGRDWINV
GRLYDPAEKDQKNVVTNGTNEMQMRAQFRAWLKYMTESM

Specific function: Degradation of benzoate to 2-hydro-1,2-dihydroxybenzoate (DHB) [H]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=426, Percent_Identity=27.4647887323944, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI1788103, Length=198, Percent_Identity=33.3333333333333, Blast_Score=105, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017639
- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.10 [H]

Molecular weight: Translated: 49895; Mature: 49764

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00070 ALDEHYDE_DEHYDR_CYS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTNTYRGNPDAIRALVRDTEVHKDTYIDQELFELEMEHLFANTWVYVGHASQVPNKGD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCC
YYTTTVGTEPVVMVRHTDDSIRVLYNRCPHKGVKVAGEVCGNTGKFFRCPYHAWTFKTDG
EEEEECCCCCEEEEEECCCHHHHHHHHCCCCCHHHHHHHCCCCCCEEECCCEEEEEECCC
SLLAIPLKKGYENTGFENCEASQGMAPVKNVKVYRDFVFCRLNPGGPSFEEYFGESLSTI
CEEEEECCCCCCCCCCCCCCCCCCCCCHHHHEEEEEEEEEEECCCCCCHHHHHHHHHHHH
DNMVDRSPEGKLEVAGGVLRYMHNCNWKMLVDNQTDTCHPMVAHESSAGTAVRVWNEAPE
HHHHHCCCCCCEEHHHHHHHHHHCCCEEEEECCCCCCCCCEEEECCCCCCEEEEECCCCC
GTPKPMAVELFAPFMSPYEFFEGMGIRVWENGHGHTGVSNSIHAAYSAIPGYWEQMVAAY
CCCCCEEHHHHHHHCCHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHCCHHHHHHHHHH
GEDRAHQILGDVRHNTVYWPNIMIKGPIQTLRLFKPIAADKTLVESWTFRLVGAPDMLLE
CCCHHHHHHHHHHCCEEECCCEEEECCHHHHHHHHHHHCCHHHHHCEEEEEECCCHHHHH
RTTMYNRLINAPTSVVGHDDLEMYERAQEGLHARGRDWINVGRLYDPAEKDQKNVVTNGT
HHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCC
NEMQMRAQFRAWLKYMTESM
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STTNTYRGNPDAIRALVRDTEVHKDTYIDQELFELEMEHLFANTWVYVGHASQVPNKGD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCC
YYTTTVGTEPVVMVRHTDDSIRVLYNRCPHKGVKVAGEVCGNTGKFFRCPYHAWTFKTDG
EEEEECCCCCEEEEEECCCHHHHHHHHCCCCCHHHHHHHCCCCCCEEECCCEEEEEECCC
SLLAIPLKKGYENTGFENCEASQGMAPVKNVKVYRDFVFCRLNPGGPSFEEYFGESLSTI
CEEEEECCCCCCCCCCCCCCCCCCCCCHHHHEEEEEEEEEEECCCCCCHHHHHHHHHHHH
DNMVDRSPEGKLEVAGGVLRYMHNCNWKMLVDNQTDTCHPMVAHESSAGTAVRVWNEAPE
HHHHHCCCCCCEEHHHHHHHHHHCCCEEEEECCCCCCCCCEEEECCCCCCEEEEECCCCC
GTPKPMAVELFAPFMSPYEFFEGMGIRVWENGHGHTGVSNSIHAAYSAIPGYWEQMVAAY
CCCCCEEHHHHHHHCCHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHCCHHHHHHHHHH
GEDRAHQILGDVRHNTVYWPNIMIKGPIQTLRLFKPIAADKTLVESWTFRLVGAPDMLLE
CCCHHHHHHHHHHCCEEECCCEEEECCHHHHHHHHHHHCCHHHHHCEEEEEECCCHHHHH
RTTMYNRLINAPTSVVGHDDLEMYERAQEGLHARGRDWINVGRLYDPAEKDQKNVVTNGT
HHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCC
NEMQMRAQFRAWLKYMTESM
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1885518 [H]