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The map label for this gene is vanB2 [H]

Identifier: 119898273

GI number: 119898273

Start: 2180606

End: 2181583

Strand: Direct

Name: vanB2 [H]

Synonym: azo1982

Alternate gene names: 119898273

Gene position: 2180606-2181583 (Clockwise)

Preceding gene: 119898272

Following gene: 119898274

Centisome position: 49.83

GC content: 69.53

Gene sequence:

>978_bases
ATGCCAGAACTGGAACTCACGATCAGCGCGATCGAGGCGGAAACGCCGCTGATCCGCACCCTTCACCTGCGGGCGCCGGC
GGGAAGCTCCCTCCCTGCCTTCACGCCCGGCGCCCACCTGCAGGTGAGCGTTCCGGGGCTGGCCGATGCGCGGTGCTACT
CCCTCGTCTCCTTCGATGCCGACCCCGCCGCCTTCGAGGCGCCGCTGGCCTACCGGCTGGGCGTGCGGCTCGAAGACGCC
AGCCGCGGCGGCTCGCGCTACATGCACGGCCTCGCCGTGGGCGACACGGTCAAGGTCAGCGGCCCGAAGAACGACTTTCC
GCTGCACGCCGCGGAAGCGGGCGAGCGCCCGATCGTGCTGATCGCCGGCGGCATCGGCATCACGCCGATCGCGGCGATGG
CGGCCTCGCTGCAGGCGTCCGGCCGCGCCTTTGAGCTGCACTACAGCGGCCGCAGCCGTGACCAGCTTGCCTTCGTCGAT
GAACTCGCCGCGCTCGCCGGCAATGCCTTCTTCGTCCATGCCGACGACGACCCCGCCAGCCGGCTCGACCTCGGCGCCCT
GCTCGATGGCCTGGACACTGCCCAGCACCTGTACGTGTGCGGCCCCAAGGGCATGATCGACGCGCTGATCGAAGGCGCGA
ATACGCGCGGCTGGCCCTCGGAGCGCGTGCATTTCGAACTTTTCGCCGCGGCCGCGCCGGTGGCGGGCGATCAGGCGTTC
GAGGTGGAGCTGGCGCAATCCGGCAAGGTGCTGCTGATTCCGGCCGACAAGACCATCCTCGACGTGATGGAGGCCGAAGG
CTGCGACCCGATGTACGACTGCAAGCGCGGCGAATGCGGCGTGTGCACCGCCACGGTGCTGGAAGGCGTGCCCGACCACC
GCGACTACTTCCTGTCCGACGCCGAAAAGGCCGGCGGCAAACTGATCCAGATCTGCATCTCGCGCGCGAAGACGCCGCGC
CTGGTGCTCGATCTCTGA

Upstream 100 bases:

>100_bases
GCTGCTCAAGGGCAACCTGACCGCCGGCAATCAACTGGCCCGCCAAGCGGCCTGAGCCACGGCAGAAAAATCGAACGGGA
CGAGAGAGGACGACACCGAA

Downstream 100 bases:

>100_bases
CCCAAGGGGGACAACGATGAGCACGACCAACACGTATCGCGGCAACCCGGACGCCATCCGCGCGCTGGTGCGCGACACCG
AGGTGCACAAGGACACCTAC

Product: vanillate O-demethylase oxidoreductase

Products: 3,4-dihydroxybenzoate; NAD+; H2O; formaldehyde

Alternate protein names: Vanillate degradation ferredoxin-like protein [H]

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MPELELTISAIEAETPLIRTLHLRAPAGSSLPAFTPGAHLQVSVPGLADARCYSLVSFDADPAAFEAPLAYRLGVRLEDA
SRGGSRYMHGLAVGDTVKVSGPKNDFPLHAAEAGERPIVLIAGGIGITPIAAMAASLQASGRAFELHYSGRSRDQLAFVD
ELAALAGNAFFVHADDDPASRLDLGALLDGLDTAQHLYVCGPKGMIDALIEGANTRGWPSERVHFELFAAAAPVAGDQAF
EVELAQSGKVLLIPADKTILDVMEAEGCDPMYDCKRGECGVCTATVLEGVPDHRDYFLSDAEKAGGKLIQICISRAKTPR
LVLDL

Sequences:

>Translated_325_residues
MPELELTISAIEAETPLIRTLHLRAPAGSSLPAFTPGAHLQVSVPGLADARCYSLVSFDADPAAFEAPLAYRLGVRLEDA
SRGGSRYMHGLAVGDTVKVSGPKNDFPLHAAEAGERPIVLIAGGIGITPIAAMAASLQASGRAFELHYSGRSRDQLAFVD
ELAALAGNAFFVHADDDPASRLDLGALLDGLDTAQHLYVCGPKGMIDALIEGANTRGWPSERVHFELFAAAAPVAGDQAF
EVELAQSGKVLLIPADKTILDVMEAEGCDPMYDCKRGECGVCTATVLEGVPDHRDYFLSDAEKAGGKLIQICISRAKTPR
LVLDL
>Mature_324_residues
PELELTISAIEAETPLIRTLHLRAPAGSSLPAFTPGAHLQVSVPGLADARCYSLVSFDADPAAFEAPLAYRLGVRLEDAS
RGGSRYMHGLAVGDTVKVSGPKNDFPLHAAEAGERPIVLIAGGIGITPIAAMAASLQASGRAFELHYSGRSRDQLAFVDE
LAALAGNAFFVHADDDPASRLDLGALLDGLDTAQHLYVCGPKGMIDALIEGANTRGWPSERVHFELFAAAAPVAGDQAFE
VELAQSGKVLLIPADKTILDVMEAEGCDPMYDCKRGECGVCTATVLEGVPDHRDYFLSDAEKAGGKLIQICISRAKTPRL
VLDL

Specific function: Unknown

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788104, Length=328, Percent_Identity=38.719512195122, Blast_Score=196, Evalue=1e-51,
Organism=Escherichia coli, GI1787658, Length=353, Percent_Identity=26.0623229461756, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788903, Length=244, Percent_Identity=28.2786885245902, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.14.13.82

Molecular weight: Translated: 34302; Mature: 34171

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELELTISAIEAETPLIRTLHLRAPAGSSLPAFTPGAHLQVSVPGLADARCYSLVSFDA
CCCEEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEECCC
DPAAFEAPLAYRLGVRLEDASRGGSRYMHGLAVGDTVKVSGPKNDFPLHAAEAGERPIVL
CCCHHCCCEEEEECEEEECCCCCCCCEEEEEEECCEEEECCCCCCCCEEHHCCCCCCEEE
IAGGIGITPIAAMAASLQASGRAFELHYSGRSRDQLAFVDELAALAGNAFFVHADDDPAS
EECCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEEECCCCCHH
RLDLGALLDGLDTAQHLYVCGPKGMIDALIEGANTRGWPSERVHFELFAAAAPVAGDQAF
HHHHHHHHHCCCCCCEEEEECCCHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCEE
EVELAQSGKVLLIPADKTILDVMEAEGCDPMYDCKRGECGVCTATVLEGVPDHRDYFLSD
EEEEECCCCEEEEECCCHHHHHHHHCCCCCHHHCCCCCCCEEHHHHHCCCCCCHHHHHCC
AEKAGGKLIQICISRAKTPRLVLDL
HHHHCCHHEEHHHHHCCCCEEEEEC
>Mature Secondary Structure 
PELELTISAIEAETPLIRTLHLRAPAGSSLPAFTPGAHLQVSVPGLADARCYSLVSFDA
CCEEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEECCC
DPAAFEAPLAYRLGVRLEDASRGGSRYMHGLAVGDTVKVSGPKNDFPLHAAEAGERPIVL
CCCHHCCCEEEEECEEEECCCCCCCCEEEEEEECCEEEECCCCCCCCEEHHCCCCCCEEE
IAGGIGITPIAAMAASLQASGRAFELHYSGRSRDQLAFVDELAALAGNAFFVHADDDPAS
EECCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEEECCCCCHH
RLDLGALLDGLDTAQHLYVCGPKGMIDALIEGANTRGWPSERVHFELFAAAAPVAGDQAF
HHHHHHHHHCCCCCCEEEEECCCHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCEE
EVELAQSGKVLLIPADKTILDVMEAEGCDPMYDCKRGECGVCTATVLEGVPDHRDYFLSD
EEEEECCCCEEEEECCCHHHHHHHHCCCCCHHHCCCCCCCEEHHHHHCCCCCCHHHHHCC
AEKAGGKLIQICISRAKTPRLVLDL
HHHHCCHHEEHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: vanillate; O2; NADH; H+

Specific reaction: vanillate + O2 + NADH + H+ = 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3170489 [H]