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The map label for this gene is thcR [H]

Identifier: 119898240

GI number: 119898240

Start: 2140807

End: 2141832

Strand: Reverse

Name: thcR [H]

Synonym: azo1949

Alternate gene names: 119898240

Gene position: 2141832-2140807 (Counterclockwise)

Preceding gene: 119898245

Following gene: 119898236

Centisome position: 48.94

GC content: 68.91

Gene sequence:

>1026_bases
ATGCTTGCCACTGGAACCGCCCCTGAATTCGCCCCACCCGCCGACCCGGCGCTGGCCCGCTTCCCGCTGTTCGAAACCCG
CGACGTGGACGAGGCGCGCGTGCGAGGCGCCCAGGTTTTCTGCGATCACAAACTGCAATTGCTCGACCGCCACAGCTCGA
TCGATGCCCGCATGTGCTTCAAGCGCCTGCGCGGCGTGGGCATCGGCCGCATGACCTACGGCGCCCACGTGGCCGTGGAG
CCGGGCTGCCTGGAGGACTTCCTGCTGGTGCAGATGCCGCTGAGCGGCGCGGAAATCCTGCGCAGCGGCGACATCGTGGT
GAATTCCAGCCGGCGCATCGCCACCGTCGCCAGTCCGCAACGTCCTTTCTCGCTGCGCCACGAAGCCGGCACCGAAAAGC
TCTTCGTCCGCATCGACCGCGACGTGCTCGAACGCCACTGCCTGCAACACCTCGGCCACGCGCTGCGCCAGCCGCTGGAG
TTTCGCCTCGACATGCCGCTGGATACCGCGGCGGGCCAGCAGTGGATGCGCGCGCTGCGCTGGCTGGTGGCCGAGCTGGA
CAGCGTGGCCGACGGCGAGGTGATCACCAGCCGCTACCCGCTGCTCGCGGCCCAGTTCGAACAGATGCTGATCTCGATGC
TGCTGGTGTGCCAGCCGCACAACTACAGCGAACAACTGCAGGCCGATGCGCCGAGCATCGCCCCGGCCTTCGTCAAGCGC
GTGGAGCGCTACATCGAGGACAACGCGCACGAACCGCTGACCATCGTCGACCTCGCCGCGCACGCCGGCGTCAGCAGCCG
CTCGCTGTTCGCCGGCTTCAAGCGCTACCGCGACACCACGCCGATGCTGCATCTGCGCGAGGTGCGCCTGCGCCGCGTGC
GTGAGGCCTTGCTGGAGGGCCGGCCGGGGCAGACTACGGTGACGAGCGAGGCGATGCGCTGGGGCTTCTCCCACCTCGGC
CACTTCACCGCCGCCTACAAGCGGCGCTTCGGCGAAAGCCCGTCGGAAACCCTGGCGCGCGGTTGA

Upstream 100 bases:

>100_bases
CCCGGCATCGGCCGCACCCGGGTGGTGCAAGACCCCGCGCCGAAGCCGCCATCGCGCGCGCCACACAAAGACAGCGCCGA
CCCCGCAGGAGAACGACGTG

Downstream 100 bases:

>100_bases
GCACGGCGGCGCGGCCGTCTCAAAAAAAAGCGGCGAGGCACGCGGCCCCGCCGTTAAAAGTGCGCAGACTGTAGCCCGCG
CACCGCCATGGAAGATGGGC

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MLATGTAPEFAPPADPALARFPLFETRDVDEARVRGAQVFCDHKLQLLDRHSSIDARMCFKRLRGVGIGRMTYGAHVAVE
PGCLEDFLLVQMPLSGAEILRSGDIVVNSSRRIATVASPQRPFSLRHEAGTEKLFVRIDRDVLERHCLQHLGHALRQPLE
FRLDMPLDTAAGQQWMRALRWLVAELDSVADGEVITSRYPLLAAQFEQMLISMLLVCQPHNYSEQLQADAPSIAPAFVKR
VERYIEDNAHEPLTIVDLAAHAGVSSRSLFAGFKRYRDTTPMLHLREVRLRRVREALLEGRPGQTTVTSEAMRWGFSHLG
HFTAAYKRRFGESPSETLARG

Sequences:

>Translated_341_residues
MLATGTAPEFAPPADPALARFPLFETRDVDEARVRGAQVFCDHKLQLLDRHSSIDARMCFKRLRGVGIGRMTYGAHVAVE
PGCLEDFLLVQMPLSGAEILRSGDIVVNSSRRIATVASPQRPFSLRHEAGTEKLFVRIDRDVLERHCLQHLGHALRQPLE
FRLDMPLDTAAGQQWMRALRWLVAELDSVADGEVITSRYPLLAAQFEQMLISMLLVCQPHNYSEQLQADAPSIAPAFVKR
VERYIEDNAHEPLTIVDLAAHAGVSSRSLFAGFKRYRDTTPMLHLREVRLRRVREALLEGRPGQTTVTSEAMRWGFSHLG
HFTAAYKRRFGESPSETLARG
>Mature_341_residues
MLATGTAPEFAPPADPALARFPLFETRDVDEARVRGAQVFCDHKLQLLDRHSSIDARMCFKRLRGVGIGRMTYGAHVAVE
PGCLEDFLLVQMPLSGAEILRSGDIVVNSSRRIATVASPQRPFSLRHEAGTEKLFVRIDRDVLERHCLQHLGHALRQPLE
FRLDMPLDTAAGQQWMRALRWLVAELDSVADGEVITSRYPLLAAQFEQMLISMLLVCQPHNYSEQLQADAPSIAPAFVKR
VERYIEDNAHEPLTIVDLAAHAGVSSRSLFAGFKRYRDTTPMLHLREVRLRRVREALLEGRPGQTTVTSEAMRWGFSHLG
HFTAAYKRRFGESPSETLARG

Specific function: Probably involved in the positive regulation of the thc operon for the degradation of the thiocarbamate herbicide EPTC [H]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788778, Length=102, Percent_Identity=37.2549019607843, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 38331; Mature: 38331

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLATGTAPEFAPPADPALARFPLFETRDVDEARVRGAQVFCDHKLQLLDRHSSIDARMCF
CCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
KRLRGVGIGRMTYGAHVAVEPGCLEDFLLVQMPLSGAEILRSGDIVVNSSRRIATVASPQ
HHHHCCCCCCEECCCEEEECCCHHHHHHHHHCCCCHHHHHHCCCEEEECCCCEEEECCCC
RPFSLRHEAGTEKLFVRIDRDVLERHCLQHLGHALRQPLEFRLDMPLDTAAGQQWMRALR
CCCCCHHHCCCCCEEEEECHHHHHHHHHHHHHHHHCCCHHEECCCCCCHHHHHHHHHHHH
WLVAELDSVADGEVITSRYPLLAAQFEQMLISMLLVCQPHNYSEQLQADAPSIAPAFVKR
HHHHHHHHCCCCCCHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHH
VERYIEDNAHEPLTIVDLAAHAGVSSRSLFAGFKRYRDTTPMLHLREVRLRRVREALLEG
HHHHHHCCCCCCEEEEEHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
RPGQTTVTSEAMRWGFSHLGHFTAAYKRRFGESPSETLARG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCC
>Mature Secondary Structure
MLATGTAPEFAPPADPALARFPLFETRDVDEARVRGAQVFCDHKLQLLDRHSSIDARMCF
CCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
KRLRGVGIGRMTYGAHVAVEPGCLEDFLLVQMPLSGAEILRSGDIVVNSSRRIATVASPQ
HHHHCCCCCCEECCCEEEECCCHHHHHHHHHCCCCHHHHHHCCCEEEECCCCEEEECCCC
RPFSLRHEAGTEKLFVRIDRDVLERHCLQHLGHALRQPLEFRLDMPLDTAAGQQWMRALR
CCCCCHHHCCCCCEEEEECHHHHHHHHHHHHHHHHCCCHHEECCCCCCHHHHHHHHHHHH
WLVAELDSVADGEVITSRYPLLAAQFEQMLISMLLVCQPHNYSEQLQADAPSIAPAFVKR
HHHHHHHHCCCCCCHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHH
VERYIEDNAHEPLTIVDLAAHAGVSSRSLFAGFKRYRDTTPMLHLREVRLRRVREALLEG
HHHHHHCCCCCCEEEEEHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
RPGQTTVTSEAMRWGFSHLGHFTAAYKRRFGESPSETLARG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]