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The map label for this gene is nmoB [H]

Identifier: 119898230

GI number: 119898230

Start: 2129059

End: 2129544

Strand: Direct

Name: nmoB [H]

Synonym: azo1939

Alternate gene names: 119898230

Gene position: 2129059-2129544 (Clockwise)

Preceding gene: 119898229

Following gene: 119898237

Centisome position: 48.65

GC content: 67.49

Gene sequence:

>486_bases
ATGTTCGACCCCCGACAGTTCCGCAACGCGCTGGGGCATTTCGCCACCGGCGTCGCCATCGTCACCACCAAAGACAGCCA
GGGCGGCGCGATCGGCCTCACCATCAACAGCTTCGCCTCGGTGTCGCTGGAGCCGCCGCTGGTGCTGTGGAGCCTCGCCC
GCAACGCGTGGAGCGTGGCTGCCTTCGAAGCCGCCGAATTCTTCGCGATCCACGTGCTGGCCGAGGACCAGCAAGATCTC
TCGAACCGCTTCGCACGCGCCTCGGAAGACAAGTTCGCCAACGTGAAGACCAGCGCGGGGCTGGGGGGGGTGCCGCTGCT
GACGGGATGCCCCGCGGTGTTCCAGTGCAGTACCGAACGCCATTACGACGGCGGCGACCACATCATCATCGTGGGGCGGG
TCGAGAGCTTCCACACCGAGGAACGGCCTCCGCTGCTGTTTCACCGGGGGCGCTATCTGGCGACGCCGGAGGGCTGCGCG
GCGTGA

Upstream 100 bases:

>100_bases
CCGCCTGAGCGTTCCCGCGTCGTCGTCTGCAAGAAAGAAGGATCGGCATCGCCGGGCCGTGGGGAGCACCTTCCCCGGCC
CGGCGGCCAAGGAGACAACC

Downstream 100 bases:

>100_bases
GGTGCTTCGCAACCCTCGTCCTGAGGCGGGTTTGACTGGAATTGCGGTGAAGACAGAAGCGCGGTGGCCGGATGGCTGGA
TTCAGGACGTAAGGCCCCGG

Product: monooxygenase component B

Products: NAD(+); reduced flavin [C]

Alternate protein names: NTA monooxygenase component B; NTA-MO B [H]

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MFDPRQFRNALGHFATGVAIVTTKDSQGGAIGLTINSFASVSLEPPLVLWSLARNAWSVAAFEAAEFFAIHVLAEDQQDL
SNRFARASEDKFANVKTSAGLGGVPLLTGCPAVFQCSTERHYDGGDHIIIVGRVESFHTEERPPLLFHRGRYLATPEGCA
A

Sequences:

>Translated_161_residues
MFDPRQFRNALGHFATGVAIVTTKDSQGGAIGLTINSFASVSLEPPLVLWSLARNAWSVAAFEAAEFFAIHVLAEDQQDL
SNRFARASEDKFANVKTSAGLGGVPLLTGCPAVFQCSTERHYDGGDHIIIVGRVESFHTEERPPLLFHRGRYLATPEGCA
A
>Mature_161_residues
MFDPRQFRNALGHFATGVAIVTTKDSQGGAIGLTINSFASVSLEPPLVLWSLARNAWSVAAFEAAEFFAIHVLAEDQQDL
SNRFARASEDKFANVKTSAGLGGVPLLTGCPAVFQCSTERHYDGGDHIIIVGRVESFHTEERPPLLFHRGRYLATPEGCA
A

Specific function: Hydroxylation of nitrilotriacetate [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081819, Length=145, Percent_Identity=29.6551724137931, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: 1.6.8.- [C]

Molecular weight: Translated: 17404; Mature: 17404

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDPRQFRNALGHFATGVAIVTTKDSQGGAIGLTINSFASVSLEPPLVLWSLARNAWSVA
CCCHHHHHHHHHHHHCCEEEEEEECCCCCEEEEEECCCEEECCCCCCHHEEHHCCCHHHH
AFEAAEFFAIHVLAEDQQDLSNRFARASEDKFANVKTSAGLGGVPLLTGCPAVFQCSTER
HHHHCEEEEEEEEECCHHHHHHHHHCCCCCCCCCEECCCCCCCCCHHHCCCHHEEECCCC
HYDGGDHIIIVGRVESFHTEERPPLLFHRGRYLATPEGCAA
CCCCCCEEEEEECCCCCCCCCCCCEEEECCEEEECCCCCCC
>Mature Secondary Structure
MFDPRQFRNALGHFATGVAIVTTKDSQGGAIGLTINSFASVSLEPPLVLWSLARNAWSVA
CCCHHHHHHHHHHHHCCEEEEEEECCCCCEEEEEECCCEEECCCCCCHHEEHHCCCHHHH
AFEAAEFFAIHVLAEDQQDLSNRFARASEDKFANVKTSAGLGGVPLLTGCPAVFQCSTER
HHHHCEEEEEEEEECCHHHHHHHHHCCCCCCCCCEECCCCCCCCCHHHCCCHHEEECCCC
HYDGGDHIIIVGRVESFHTEERPPLLFHRGRYLATPEGCAA
CCCCCCEEEEEECCCCCCCCCCCCEEEECCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; flavin [C]

Specific reaction: NADH + flavin = NAD(+) + reduced flavin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8892809; 9023192; 1735711 [H]