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The map label for this gene is lapG

Identifier: 119898148

GI number: 119898148

Start: 2039462

End: 2040505

Strand: Direct

Name: lapG

Synonym: azo1857

Alternate gene names: 119898148

Gene position: 2039462-2040505 (Clockwise)

Preceding gene: 119898147

Following gene: 119898149

Centisome position: 46.61

GC content: 67.34

Gene sequence:

>1044_bases
ATGAGCGAATCGAACCTGAAGGGGCGCAAGGTCGTCCTGCACGACATGTGTCTGCGCGACGGCATGCACGCCAAGCGCGA
GCAGATCAGCGTGGAGCAGATGGTGAAGGTGGCCACCGCGCTGGACGACGCCGGCATTCCCTACCTGCAGGTGACCCACG
GCGCCGGGCTGGGCGGCAATTCGCTGCAGCACGGCTTTGCGCTGGCGAGCAACGAGGAATACATCGCCGCGGTGGCGTCG
CGGATGAAGCAGGCCAAGGTGTCGGTGCTGCTGATTCCCGGCCTGGGCACGATGCGCGAGCTGCAGGCGGCCTACGACGC
GGGTGCCCGCAGCGTGCATGTGGCCACCCACTGCACCGAGGCCGACACCGCGCCCCAGCACATCGCCTTCGCCCGCAAGC
TGGGCATGGATACGACCGGCTTCCTGATGATGTCCCACCTCAACGACGCCGCCGGCATTGCCCAGCAGGGCAAGCTGATG
GAGTCCTACGGCGCGCAAACGGTGTACGTGACCGACTCCGCCGGCTACATGCTGCCCGCCGACGTCACCGCCCGCGTGCG
CGCGCTGCGCGAGGTGCTGAACCCGGAAACCGAGATCGGCTTCCACGGCCATCACAACCTGGGCATGGGCATCGCCAATT
CCATCGCCGCGATCGAGGCCGGCGCGACTCGCATCGACGGCTCGGTGGCCGGCCTGGGCGCCGGCGCCGGCAACACGCCG
CTGGAAGTGTTCGCCGCGGTGTGCGAGCGGATGGGCATCGAAACCGGCGTCGATCTCTTCAAGCTGATGGACGTGGCCGA
GGACGTGATCGTGCCGATGATGGACCACATGGTCCGCGTGGATCGGGAATCGCTGACGCTGGGGTTTGCGGGGGTGTATT
CCACCTTCCTGTTGCATGCGAAGCGTGCGGCGGAGCGCTTTGGGGTGCCGGCGCGGGACATCCTGGTCGAGCTGGGCAGA
AAGAAGATGATCGGCGGGCAGGAAGACATGATCCAGGACACCGCGATGACGATGGCGCGGGAGCGCGGGGTGAGTGCGGC
CTGA

Upstream 100 bases:

>100_bases
ATCTGGACATCATGACCGCGGCCGCGACCCGCACCGCCGAGATGTTCGCCGAGGAGATCCTGGCCGGAAAGATCAAGCTG
AAGACCGCGGAGGTGGCGTG

Downstream 100 bases:

>100_bases
CACAGCCGGAAGCGCTGGCTGGATTGAGGATGAACTTCAGCGGGCCACGGGTGTAGCCCCTTCCCCTTCAAGGGGAAGGT
TGGGATGGGGTTCTAACAGG

Product: 4-hydroxy-2-ketovalerate aldolase

Products: NA

Alternate protein names: HOA 1; 4-hydroxy-2-keto-pentanoic acid aldolase 1; 4-hydroxy-2-oxopentanoate aldolase 1

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MSESNLKGRKVVLHDMCLRDGMHAKREQISVEQMVKVATALDDAGIPYLQVTHGAGLGGNSLQHGFALASNEEYIAAVAS
RMKQAKVSVLLIPGLGTMRELQAAYDAGARSVHVATHCTEADTAPQHIAFARKLGMDTTGFLMMSHLNDAAGIAQQGKLM
ESYGAQTVYVTDSAGYMLPADVTARVRALREVLNPETEIGFHGHHNLGMGIANSIAAIEAGATRIDGSVAGLGAGAGNTP
LEVFAAVCERMGIETGVDLFKLMDVAEDVIVPMMDHMVRVDRESLTLGFAGVYSTFLLHAKRAAERFGVPARDILVELGR
KKMIGGQEDMIQDTAMTMARERGVSAA

Sequences:

>Translated_347_residues
MSESNLKGRKVVLHDMCLRDGMHAKREQISVEQMVKVATALDDAGIPYLQVTHGAGLGGNSLQHGFALASNEEYIAAVAS
RMKQAKVSVLLIPGLGTMRELQAAYDAGARSVHVATHCTEADTAPQHIAFARKLGMDTTGFLMMSHLNDAAGIAQQGKLM
ESYGAQTVYVTDSAGYMLPADVTARVRALREVLNPETEIGFHGHHNLGMGIANSIAAIEAGATRIDGSVAGLGAGAGNTP
LEVFAAVCERMGIETGVDLFKLMDVAEDVIVPMMDHMVRVDRESLTLGFAGVYSTFLLHAKRAAERFGVPARDILVELGR
KKMIGGQEDMIQDTAMTMARERGVSAA
>Mature_346_residues
SESNLKGRKVVLHDMCLRDGMHAKREQISVEQMVKVATALDDAGIPYLQVTHGAGLGGNSLQHGFALASNEEYIAAVASR
MKQAKVSVLLIPGLGTMRELQAAYDAGARSVHVATHCTEADTAPQHIAFARKLGMDTTGFLMMSHLNDAAGIAQQGKLME
SYGAQTVYVTDSAGYMLPADVTARVRALREVLNPETEIGFHGHHNLGMGIANSIAAIEAGATRIDGSVAGLGAGAGNTPL
EVFAAVCERMGIETGVDLFKLMDVAEDVIVPMMDHMVRVDRESLTLGFAGVYSTFLLHAKRAAERFGVPARDILVELGRK
KMIGGQEDMIQDTAMTMARERGVSAA

Specific function: Catalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 4-hydroxy-2-oxovalerate aldolase family

Homologues:

Organism=Escherichia coli, GI1786548, Length=337, Percent_Identity=54.3026706231454, Blast_Score=370, Evalue=1e-104,
Organism=Escherichia coli, GI1786261, Length=262, Percent_Identity=27.4809160305344, Blast_Score=80, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320071, Length=245, Percent_Identity=26.530612244898, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6320019, Length=236, Percent_Identity=27.1186440677966, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HOA1_AZOSB (A1K6L9)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_933361.1
- ProteinModelPortal:   A1K6L9
- SMR:   A1K6L9
- STRING:   A1K6L9
- GeneID:   4609113
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo1857
- eggNOG:   COG0119
- HOGENOM:   HBG300122
- OMA:   GMHAKRE
- ProtClustDB:   PRK08195
- BioCyc:   ASP62928:AZO1857-MONOMER
- HAMAP:   MF_01656
- InterPro:   IPR017629
- InterPro:   IPR013785
- InterPro:   IPR012425
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- ProDom:   PD005364
- TIGRFAMs:   TIGR03217

Pfam domain/function: PF07836 DmpG_comm; PF00682 HMGL-like

EC number: =4.1.3.39

Molecular weight: Translated: 36980; Mature: 36849

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS50991 PYR_CT

Important sites: BINDING 173-173 BINDING 202-202 BINDING 293-293

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
6.1 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESNLKGRKVVLHDMCLRDGMHAKREQISVEQMVKVATALDDAGIPYLQVTHGAGLGGN
CCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
SLQHGFALASNEEYIAAVASRMKQAKVSVLLIPGLGTMRELQAAYDAGARSVHVATHCTE
CHHCCEEECCCCHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHCCCCEEEEEEECCC
ADTAPQHIAFARKLGMDTTGFLMMSHLNDAAGIAQQGKLMESYGAQTVYVTDSAGYMLPA
CCCCHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHCHHHHHHCCCEEEEECCCCCEECC
DVTARVRALREVLNPETEIGFHGHHNLGMGIANSIAAIEAGATRIDGSVAGLGAGAGNTP
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEECCCCCCCCH
LEVFAAVCERMGIETGVDLFKLMDVAEDVIVPMMDHMVRVDRESLTLGFAGVYSTFLLHA
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHEEHHHHHHHHHHHHH
KRAAERFGVPARDILVELGRKKMIGGQEDMIQDTAMTMARERGVSAA
HHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SESNLKGRKVVLHDMCLRDGMHAKREQISVEQMVKVATALDDAGIPYLQVTHGAGLGGN
CCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
SLQHGFALASNEEYIAAVASRMKQAKVSVLLIPGLGTMRELQAAYDAGARSVHVATHCTE
CHHCCEEECCCCHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHCCCCEEEEEEECCC
ADTAPQHIAFARKLGMDTTGFLMMSHLNDAAGIAQQGKLMESYGAQTVYVTDSAGYMLPA
CCCCHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHCHHHHHHCCCEEEEECCCCCEECC
DVTARVRALREVLNPETEIGFHGHHNLGMGIANSIAAIEAGATRIDGSVAGLGAGAGNTP
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEECCCCCCCCH
LEVFAAVCERMGIETGVDLFKLMDVAEDVIVPMMDHMVRVDRESLTLGFAGVYSTFLLHA
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHEEHHHHHHHHHHHHH
KRAAERFGVPARDILVELGRKKMIGGQEDMIQDTAMTMARERGVSAA
HHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA