Click here to switch to the map view.

The map label for this gene is lapR [H]

Identifier: 119898133

GI number: 119898133

Start: 2024002

End: 2025687

Strand: Direct

Name: lapR [H]

Synonym: azo1842

Alternate gene names: 119898133

Gene position: 2024002-2025687 (Clockwise)

Preceding gene: 119898132

Following gene: 119898134

Centisome position: 46.25

GC content: 68.39

Gene sequence:

>1686_bases
ATGGGGACTGAAAAACACGGGCTGGTCGAGCAGTTCAACCTGCGTTCGCGGCTGCGCTTCGATATCGACGGCGGCAACAT
CTGGCTGGATGAGAGCCGCATGCTGCTGCTGCACGCGCAGGCGATGGGCGCCCTGCGCACCGAGCTGTTCCGATCGCTGG
GCGTGGAGCGCGCGCGCGGCCTGCTGGTGCGCATGGGCTTCGTCTCCGGGCAGCAGGACGCCGACCTCGCGCGCAAGCTG
TTCGGCAACGGCGAACCCTACGACGTGTTCCGCCTCGGGCCGGAACTGCACGCCTTCGAAGGCCTGGTGCGGGCGCGTAT
CCGCGACGCGAAACTGGACTGGGAAAAAGGCAGCTTCGTCGGCGACGTCACCTGGGAGAACTCCTGGGAGGCGGAGTCCC
ACATCCGCGAATTCGGTGTCGGCGACGACCCGGTGTGCTGGAGCCTGATCGGCTATGCCTCCGGTTATGTCACCCGCTTC
TTCAACCGCTTCGTGGTGTTCCGCGAGACGCACTGCGTCGGCAAGGGCGACGAACAGTGCGTGCTGGTCGGCAAGCCGGC
CGAGGACTGGGACAACGCCGACGGCTACCTCGACTACTTCCGCCCCGGCAACATCGAGGGCGAACTCGCCGAACTGCGCG
AAGAACTCGCGCGCCTGCGGGCCTCGCTCGGCGGCGCCTGCCGGCGCGACGACCTGATCGGCAATTCCCCCGCCTTCGAG
GCCGCCTTCGACCTGGTCCGCAAGGCGGCGGGCAGCACCATCAACGTGCTGCTGCTCGGCGAAACCGGCGTCGGCAAGGA
AGTCTTCGCGCGCTGGCTGCATGCCCACAGCGACCGCGCCGACAAGCCCTTCATCGCGATCAACTGCGCGGCGATCCCGC
ACGACCTGATCGAAGCCGAACTCTTCGGCGTGCAGAAGGGCGCCTACACCGGCGCCCAGCAATCCCGCGCCGGCCGCTTC
GAACGCGCCGACGGCGGCACGCTGTTCCTCGACGAGGTCGGCGACCTGTCGCCGGCGGCGCAGGTGAAGCTGCTGCGCGT
GCTGCAGTCCGGCGAGGTGGAACGCCTCGGCGACGACCAGACGCGCAAGGTGAACGTGCGCCTGGTGGCGGCCACCAACG
TCAATCTGCCGCAGGCGATCCGCCAGGGCAGCTTCCGCGCCGACCTCTACTACCGGTTGGCCACCTACCCGGTGGCCATC
CCGCCGCTGCGCGACCGCCGCAGCGACATCCCGCTGCTCGCCCAGGCCCTGCTCGGCAAGTACGAGGCGGTCTATCACAA
GAAGCTGCAAGGCATCACCGACCGCGCCATCCGCGCGCTGGTCGATTACCCCTGGCCGGGCAACGTGCGCGAACTGGAAA
ACGTGATCGAACGCGGCGTGCTGCTGGCACCGCCCGGCGGACGGCTGGAGGAACAGCACCTGTTCGCCGGCGCGGAGTCG
GTGGTGCCTGCCGGCGTGGAACTCGATACCGGCGGGCATCTGTGCGACGCGAGGGAGAGTTCGCACAACAAGCTGTGCGA
GGCGCTGCTGAAGGAGGGTTTCAACCTCGAAGCGCACGAGGCGCGGCTGATCGAACTCGCCGTGCAGCAGGCCAAGGGGA
ATCTCACGCATGCGGCCAGGGCGCTGGGGATTACGCGGCGGCAGCTGGCCTATCGGTTGAAGCAGAACACGGCCGCGGCG
GAGTAG

Upstream 100 bases:

>100_bases
CGTGGCGGGCAGCGCGAGGCTATTACGATCGCGGTAAAGTGCGAGGCCTATGGAAGAACACGGTCAGGCCCCCGGCAGAG
GGGCGCGACCCGGAGGCGTG

Downstream 100 bases:

>100_bases
GGGAGGGCGCGGGCGCGGGGGCAGGCGAGGGATTCAGGCGCGGGATTCAGGCGCGGGATTCAGGCGCGGGATTCAGGCGC
GGGATTCAGGCGAGGGATTC

Product: transcriptional regulator

Products: NA

Alternate protein names: 67 kDa protein [H]

Number of amino acids: Translated: 561; Mature: 560

Protein sequence:

>561_residues
MGTEKHGLVEQFNLRSRLRFDIDGGNIWLDESRMLLLHAQAMGALRTELFRSLGVERARGLLVRMGFVSGQQDADLARKL
FGNGEPYDVFRLGPELHAFEGLVRARIRDAKLDWEKGSFVGDVTWENSWEAESHIREFGVGDDPVCWSLIGYASGYVTRF
FNRFVVFRETHCVGKGDEQCVLVGKPAEDWDNADGYLDYFRPGNIEGELAELREELARLRASLGGACRRDDLIGNSPAFE
AAFDLVRKAAGSTINVLLLGETGVGKEVFARWLHAHSDRADKPFIAINCAAIPHDLIEAELFGVQKGAYTGAQQSRAGRF
ERADGGTLFLDEVGDLSPAAQVKLLRVLQSGEVERLGDDQTRKVNVRLVAATNVNLPQAIRQGSFRADLYYRLATYPVAI
PPLRDRRSDIPLLAQALLGKYEAVYHKKLQGITDRAIRALVDYPWPGNVRELENVIERGVLLAPPGGRLEEQHLFAGAES
VVPAGVELDTGGHLCDARESSHNKLCEALLKEGFNLEAHEARLIELAVQQAKGNLTHAARALGITRRQLAYRLKQNTAAA
E

Sequences:

>Translated_561_residues
MGTEKHGLVEQFNLRSRLRFDIDGGNIWLDESRMLLLHAQAMGALRTELFRSLGVERARGLLVRMGFVSGQQDADLARKL
FGNGEPYDVFRLGPELHAFEGLVRARIRDAKLDWEKGSFVGDVTWENSWEAESHIREFGVGDDPVCWSLIGYASGYVTRF
FNRFVVFRETHCVGKGDEQCVLVGKPAEDWDNADGYLDYFRPGNIEGELAELREELARLRASLGGACRRDDLIGNSPAFE
AAFDLVRKAAGSTINVLLLGETGVGKEVFARWLHAHSDRADKPFIAINCAAIPHDLIEAELFGVQKGAYTGAQQSRAGRF
ERADGGTLFLDEVGDLSPAAQVKLLRVLQSGEVERLGDDQTRKVNVRLVAATNVNLPQAIRQGSFRADLYYRLATYPVAI
PPLRDRRSDIPLLAQALLGKYEAVYHKKLQGITDRAIRALVDYPWPGNVRELENVIERGVLLAPPGGRLEEQHLFAGAES
VVPAGVELDTGGHLCDARESSHNKLCEALLKEGFNLEAHEARLIELAVQQAKGNLTHAARALGITRRQLAYRLKQNTAAA
E
>Mature_560_residues
GTEKHGLVEQFNLRSRLRFDIDGGNIWLDESRMLLLHAQAMGALRTELFRSLGVERARGLLVRMGFVSGQQDADLARKLF
GNGEPYDVFRLGPELHAFEGLVRARIRDAKLDWEKGSFVGDVTWENSWEAESHIREFGVGDDPVCWSLIGYASGYVTRFF
NRFVVFRETHCVGKGDEQCVLVGKPAEDWDNADGYLDYFRPGNIEGELAELREELARLRASLGGACRRDDLIGNSPAFEA
AFDLVRKAAGSTINVLLLGETGVGKEVFARWLHAHSDRADKPFIAINCAAIPHDLIEAELFGVQKGAYTGAQQSRAGRFE
RADGGTLFLDEVGDLSPAAQVKLLRVLQSGEVERLGDDQTRKVNVRLVAATNVNLPQAIRQGSFRADLYYRLATYPVAIP
PLRDRRSDIPLLAQALLGKYEAVYHKKLQGITDRAIRALVDYPWPGNVRELENVIERGVLLAPPGGRLEEQHLFAGAESV
VPAGVELDTGGHLCDARESSHNKLCEALLKEGFNLEAHEARLIELAVQQAKGNLTHAARALGITRRQLAYRLKQNTAAAE

Specific function: Regulatory protein of the TOL plasmid xyl operons. In the presence of m-xylene or m-methylbenzyl alcohol xylR activates both the xylCMABN operon and the regulatory gene xylS; xylS itself activates the xylXYZLTEGFJQKIH operon. XylR interacts with sigma- 54

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=348, Percent_Identity=41.6666666666667, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1790437, Length=367, Percent_Identity=40.5994550408719, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI87082117, Length=288, Percent_Identity=48.9583333333333, Blast_Score=248, Evalue=6e-67,
Organism=Escherichia coli, GI1789087, Length=275, Percent_Identity=45.8181818181818, Blast_Score=228, Evalue=1e-60,
Organism=Escherichia coli, GI1790299, Length=336, Percent_Identity=41.9642857142857, Blast_Score=225, Evalue=6e-60,
Organism=Escherichia coli, GI87081872, Length=341, Percent_Identity=40.1759530791789, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1788905, Length=328, Percent_Identity=41.1585365853659, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI87082152, Length=333, Percent_Identity=41.4414414414414, Blast_Score=218, Evalue=9e-58,
Organism=Escherichia coli, GI1789233, Length=238, Percent_Identity=43.2773109243698, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1786524, Length=339, Percent_Identity=37.4631268436578, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1787583, Length=232, Percent_Identity=39.6551724137931, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI87081858, Length=314, Percent_Identity=32.1656050955414, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1789828, Length=285, Percent_Identity=33.6842105263158, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR004096
- InterPro:   IPR010523
- ProDom:   PD485437 [H]

Pfam domain/function: PF02954 HTH_8; PF00158 Sigma54_activat; PF02830 V4R; PF06505 XylR_N [H]

EC number: NA

Molecular weight: Translated: 62129; Mature: 61998

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTEKHGLVEQFNLRSRLRFDIDGGNIWLDESRMLLLHAQAMGALRTELFRSLGVERARG
CCCCCCCHHHHHCCCCEEEEEECCCEEEECCCCEEEEEHHHHHHHHHHHHHHHCHHHHCC
LLVRMGFVSGQQDADLARKLFGNGEPYDVFRLGPELHAFEGLVRARIRDAKLDWEKGSFV
EEEEEECCCCCCHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCEE
GDVTWENSWEAESHIREFGVGDDPVCWSLIGYASGYVTRFFNRFVVFRETHCVGKGDEQC
EEECCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCE
VLVGKPAEDWDNADGYLDYFRPGNIEGELAELREELARLRASLGGACRRDDLIGNSPAFE
EEECCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
AAFDLVRKAAGSTINVLLLGETGVGKEVFARWLHAHSDRADKPFIAINCAAIPHDLIEAE
HHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHH
LFGVQKGAYTGAQQSRAGRFERADGGTLFLDEVGDLSPAAQVKLLRVLQSGEVERLGDDQ
HHHCCCCCCCCCHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCHHHCCCCC
TRKVNVRLVAATNVNLPQAIRQGSFRADLYYRLATYPVAIPPLRDRRSDIPLLAQALLGK
CEEEEEEEEEEECCCHHHHHHCCCCCHHHEEEEECCCEECCCCCCCCCCCHHHHHHHHHH
YEAVYHKKLQGITDRAIRALVDYPWPGNVRELENVIERGVLLAPPGGRLEEQHLFAGAES
HHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHHHHHCCEEECCCCCCCCHHHHHCCCHH
VVPAGVELDTGGHLCDARESSHNKLCEALLKEGFNLEAHEARLIELAVQQAKGNLTHAAR
CCCCCEEECCCCCEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHH
ALGITRRQLAYRLKQNTAAAE
HHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GTEKHGLVEQFNLRSRLRFDIDGGNIWLDESRMLLLHAQAMGALRTELFRSLGVERARG
CCCCCCHHHHHCCCCEEEEEECCCEEEECCCCEEEEEHHHHHHHHHHHHHHHCHHHHCC
LLVRMGFVSGQQDADLARKLFGNGEPYDVFRLGPELHAFEGLVRARIRDAKLDWEKGSFV
EEEEEECCCCCCHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCEE
GDVTWENSWEAESHIREFGVGDDPVCWSLIGYASGYVTRFFNRFVVFRETHCVGKGDEQC
EEECCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCE
VLVGKPAEDWDNADGYLDYFRPGNIEGELAELREELARLRASLGGACRRDDLIGNSPAFE
EEECCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
AAFDLVRKAAGSTINVLLLGETGVGKEVFARWLHAHSDRADKPFIAINCAAIPHDLIEAE
HHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHH
LFGVQKGAYTGAQQSRAGRFERADGGTLFLDEVGDLSPAAQVKLLRVLQSGEVERLGDDQ
HHHCCCCCCCCCHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCHHHCCCCC
TRKVNVRLVAATNVNLPQAIRQGSFRADLYYRLATYPVAIPPLRDRRSDIPLLAQALLGK
CEEEEEEEEEEECCCHHHHHHCCCCCHHHEEEEECCCEECCCCCCCCCCCHHHHHHHHHH
YEAVYHKKLQGITDRAIRALVDYPWPGNVRELENVIERGVLLAPPGGRLEEQHLFAGAES
HHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHHHHHCCEEECCCCCCCCHHHHHCCCHH
VVPAGVELDTGGHLCDARESSHNKLCEALLKEGFNLEAHEARLIELAVQQAKGNLTHAAR
CCCCCEEECCCCCEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHH
ALGITRRQLAYRLKQNTAAAE
HHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3169574; 2993247; 2430049 [H]