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The map label for this gene is alkA [H]

Identifier: 119898124

GI number: 119898124

Start: 2011690

End: 2012379

Strand: Direct

Name: alkA [H]

Synonym: azo1833

Alternate gene names: 119898124

Gene position: 2011690-2012379 (Clockwise)

Preceding gene: 119898123

Following gene: 119898127

Centisome position: 45.97

GC content: 71.01

Gene sequence:

>690_bases
ATGGATACACCCGACACCCTACCGCCCGCGCAGGCCGAGCTGTACCAGCTGGCCACCGCGCACCTGGCCGGCATCGACGC
GGACTGGGCGCGCCTGGTGACCGCCGTCGGCCCCTGCCTGCTGCAACCCAAGCCGGCCCGCGAACCTTACGAGGCGCTGG
TGCGCGCTGTCGCCTACCAGCAGCTCGCCACCAGCGTGGGCGACCGCATCATTGGCCGCCTGCTGGCGCTCTATCCAGAC
AGCGCGTTTCCGCAACCGGAACAACTGCTCGCGACCGGCTTCGATGCGCTGCGCGGCTGCGGCTTTTCCGCCCGCAAGAT
CGAAACCATCCACGGCATCGCGCAGGGCACGCTGAGCGGCCTGGTGCCGAGCCGGGCGGATGCGGTGTCGATGGACGACG
AGGCGCTGATCGCCCGCCTGGTGGAACTGCGCGGCATTGGCCGCTGGACGGTGGAGATGCTGCTGATCTTCACGCTGGAG
CGGATCGACGTGCTGCCGGTGGACGACTTCGGCGTGCGCGAGGGCTACCGCCACCTGAAGTCGCTGGACGAGATGCCGGG
CCGCAAGGAGATGGCGCGCGCCGGGCTGGTGTGCAGCCCCTATCGCACGGTGGCCGCGTGGTATCTGTGGCGGTCGCTGG
CGCTGCCGGACTACCGCCGGCGGCGCGCCGGCAAACCGGGCGCCCCCTGA

Upstream 100 bases:

>100_bases
GGAAGCCGTTGCAGCCGAATCCGGATTCGACAGCAGGAATCGGGATGCCCACTTCGGCAAGCAGCGGGCATCATGAAGGC
CCGAGTTCGCATCCCGGCTG

Downstream 100 bases:

>100_bases
GCCCGTGCAACCGCGGTGGCGCCCGGCCGCCCGGAGCCGCTGAGCGGGTCGGCCGTGCCGAGCCGACCCTTCGCCGACGC
GGCGAGCGCGGCAGCCTCTC

Product: DNA-3-methyladenine glycosylase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MDTPDTLPPAQAELYQLATAHLAGIDADWARLVTAVGPCLLQPKPAREPYEALVRAVAYQQLATSVGDRIIGRLLALYPD
SAFPQPEQLLATGFDALRGCGFSARKIETIHGIAQGTLSGLVPSRADAVSMDDEALIARLVELRGIGRWTVEMLLIFTLE
RIDVLPVDDFGVREGYRHLKSLDEMPGRKEMARAGLVCSPYRTVAAWYLWRSLALPDYRRRRAGKPGAP

Sequences:

>Translated_229_residues
MDTPDTLPPAQAELYQLATAHLAGIDADWARLVTAVGPCLLQPKPAREPYEALVRAVAYQQLATSVGDRIIGRLLALYPD
SAFPQPEQLLATGFDALRGCGFSARKIETIHGIAQGTLSGLVPSRADAVSMDDEALIARLVELRGIGRWTVEMLLIFTLE
RIDVLPVDDFGVREGYRHLKSLDEMPGRKEMARAGLVCSPYRTVAAWYLWRSLALPDYRRRRAGKPGAP
>Mature_229_residues
MDTPDTLPPAQAELYQLATAHLAGIDADWARLVTAVGPCLLQPKPAREPYEALVRAVAYQQLATSVGDRIIGRLLALYPD
SAFPQPEQLLATGFDALRGCGFSARKIETIHGIAQGTLSGLVPSRADAVSMDDEALIARLVELRGIGRWTVEMLLIFTLE
RIDVLPVDDFGVREGYRHLKSLDEMPGRKEMARAGLVCSPYRTVAAWYLWRSLALPDYRRRRAGKPGAP

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, 3-methylguanine, 7-methylguanine, O2- methylthymine, and O2-methylcytosine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkylbase DNA glycosidase alkA family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00730 HhH-GPD [H]

EC number: =3.2.2.21 [H]

Molecular weight: Translated: 25179; Mature: 25179

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTPDTLPPAQAELYQLATAHLAGIDADWARLVTAVGPCLLQPKPAREPYEALVRAVAYQ
CCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
QLATSVGDRIIGRLLALYPDSAFPQPEQLLATGFDALRGCGFSARKIETIHGIAQGTLSG
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LVPSRADAVSMDDEALIARLVELRGIGRWTVEMLLIFTLERIDVLPVDDFGVREGYRHLK
CCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
SLDEMPGRKEMARAGLVCSPYRTVAAWYLWRSLALPDYRRRRAGKPGAP
HHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCC
>Mature Secondary Structure
MDTPDTLPPAQAELYQLATAHLAGIDADWARLVTAVGPCLLQPKPAREPYEALVRAVAYQ
CCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
QLATSVGDRIIGRLLALYPDSAFPQPEQLLATGFDALRGCGFSARKIETIHGIAQGTLSG
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LVPSRADAVSMDDEALIARLVELRGIGRWTVEMLLIFTLERIDVLPVDDFGVREGYRHLK
CCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
SLDEMPGRKEMARAGLVCSPYRTVAAWYLWRSLALPDYRRRRAGKPGAP
HHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]