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The map label for this gene is rpoN2 [H]

Identifier: 119898081

GI number: 119898081

Start: 1957602

End: 1959068

Strand: Reverse

Name: rpoN2 [H]

Synonym: azo1790

Alternate gene names: 119898081

Gene position: 1959068-1957602 (Counterclockwise)

Preceding gene: 119898091

Following gene: 119898080

Centisome position: 44.77

GC content: 69.53

Gene sequence:

>1467_bases
ATGAGCCTCGGAATGGAGCTTCGCCTGCGTCAGCACCTGGCCATCACGCCGCAGATCCAGCAAGCCTTGCGCCTGCTGCA
ACTGTCGTCGCAGGAGTTCACCCAGGAAGTCGAACAAGCGGTCACCAGCAACCCCTTCCTGGAGGAAGATCCAGACGCCC
CGCCCCCCGGCCCCGCGGCCGCGATGCCGCTGGCCCCGGCGGCGTCTTCCAGCGAGCCGCGCGCGGAAACCGTCGATGGC
GGCGACGGAGAAGGCAGCGACGCGCCGGTGTCGCCCGCGCTGCACGAAATCGGCCGCAGCGGCGGCGGCGGGGGCGGCGA
CGAGGAGCACGACTGGACCGAATGGACAGAGGCGCCGATCAGCCTGCGCGACAACCTGCGCAACCAGCTGCTGCTGTCGC
CGATGAGCGACCGCGACCGCACGCTGGCGCACATGGTGATCGACGAACTGACCGACGCCGGCTACCTGGAAACGCCGCTG
GAAGAACTGGCGGACCTGATCTCGCCCGAGGAAGAGGTGGACGTGCACGAGCTGAGCGCCGCGCTGAAGCTGGTGCAGCA
GCTCGACCCGCCCGGCATCGCCGCGCGCTCGCTGCAGGAATGCCTGCTGCTGCAATTGCAGGCGCTGCCCGCCTCCACCC
CCAACCTGTCGCTGGCGCTGGAACTCGTGGGTCAGCACTTCGAACTGCTGGCGCGGCGCGAATTCACCCGTCTGCAGCAG
CTGCTGAGCTGCAACGAGAACGCGCTGCACGGCGCCCGCGCGCTGATCCGCACACTGGACCCGCGCCCCGGGCGCTGCTT
CGCCCCGGACAACACCCGCTACGTGGTGCCCGACATCATCGTCACCAACCAGCGCGGACGCTGGGTGGCGACGCTGAACC
CGGCGGTGCAGCCGCAAGTGCGGATCAACCGCGCCTATGCCGAAATCGCCGCCAGCCGCAGCGGCTGCGGCGCCTCGTTC
TCGCGCCTGCTGCAGGAAGCGCGCTGGCTGCTGCGCAACGTGGAGCAGCGTTTCAACACCATCCAGCGCGTGGCCGACGC
CATCGTCGCGCGCCAGCGGGTGTTCTTCAGCTACGGCGAGGCGGCGATGAAGCCGATGGCGCTGAAGGACATCGCCACCG
AGCTGGGCCTGCACGAATCGACCGTGTGCCGCGTCACCAACGGCAAGTACATGGCCACCCCGCGGGGGCTGTTCGAGTTC
AAGTATTTCTTTTCGCGCCAGCTTGCCACCGACGATGGCGGCAGCTGCTCGGCGACCGCGATCCGCGCCCTCCTCAAAGA
GCTGATCGCCGCCGAGGACCCCCAGACCCCGCTGTCGGACGCCCAGCTCGCGCGCATGCTCGCCGAGCAAGGGCTGCGTC
TGGCGCGCCGCACAGTCACCAAGTACCGCACCCTGATGCGCGTACCGAGCGTGGAGCTGCGTCGCGTTGCGGGTCGCGCA
CCCAACGCGGCCGAAGCGGGGGCCTGA

Upstream 100 bases:

>100_bases
CTTCAGCGCGATGGCATGGACCTTGCGCGCAGGTGGTCGACCCGCCTTTTCCCCAGCGGAGCAGACGAATGTCACTCGAC
CAGAACACGAAGGAGACACC

Downstream 100 bases:

>100_bases
GCGCCCCCGCCGCGTCGCCTACTTGCCGGAGGCTGCCATGGCAACGATCATCGCCGGTCATTTCGACCACGTCGAACATC
TGAACGAAGCGATCAGGCTG

Product: RNA polymerase sigma-54 factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MSLGMELRLRQHLAITPQIQQALRLLQLSSQEFTQEVEQAVTSNPFLEEDPDAPPPGPAAAMPLAPAASSSEPRAETVDG
GDGEGSDAPVSPALHEIGRSGGGGGGDEEHDWTEWTEAPISLRDNLRNQLLLSPMSDRDRTLAHMVIDELTDAGYLETPL
EELADLISPEEEVDVHELSAALKLVQQLDPPGIAARSLQECLLLQLQALPASTPNLSLALELVGQHFELLARREFTRLQQ
LLSCNENALHGARALIRTLDPRPGRCFAPDNTRYVVPDIIVTNQRGRWVATLNPAVQPQVRINRAYAEIAASRSGCGASF
SRLLQEARWLLRNVEQRFNTIQRVADAIVARQRVFFSYGEAAMKPMALKDIATELGLHESTVCRVTNGKYMATPRGLFEF
KYFFSRQLATDDGGSCSATAIRALLKELIAAEDPQTPLSDAQLARMLAEQGLRLARRTVTKYRTLMRVPSVELRRVAGRA
PNAAEAGA

Sequences:

>Translated_488_residues
MSLGMELRLRQHLAITPQIQQALRLLQLSSQEFTQEVEQAVTSNPFLEEDPDAPPPGPAAAMPLAPAASSSEPRAETVDG
GDGEGSDAPVSPALHEIGRSGGGGGGDEEHDWTEWTEAPISLRDNLRNQLLLSPMSDRDRTLAHMVIDELTDAGYLETPL
EELADLISPEEEVDVHELSAALKLVQQLDPPGIAARSLQECLLLQLQALPASTPNLSLALELVGQHFELLARREFTRLQQ
LLSCNENALHGARALIRTLDPRPGRCFAPDNTRYVVPDIIVTNQRGRWVATLNPAVQPQVRINRAYAEIAASRSGCGASF
SRLLQEARWLLRNVEQRFNTIQRVADAIVARQRVFFSYGEAAMKPMALKDIATELGLHESTVCRVTNGKYMATPRGLFEF
KYFFSRQLATDDGGSCSATAIRALLKELIAAEDPQTPLSDAQLARMLAEQGLRLARRTVTKYRTLMRVPSVELRRVAGRA
PNAAEAGA
>Mature_487_residues
SLGMELRLRQHLAITPQIQQALRLLQLSSQEFTQEVEQAVTSNPFLEEDPDAPPPGPAAAMPLAPAASSSEPRAETVDGG
DGEGSDAPVSPALHEIGRSGGGGGGDEEHDWTEWTEAPISLRDNLRNQLLLSPMSDRDRTLAHMVIDELTDAGYLETPLE
ELADLISPEEEVDVHELSAALKLVQQLDPPGIAARSLQECLLLQLQALPASTPNLSLALELVGQHFELLARREFTRLQQL
LSCNENALHGARALIRTLDPRPGRCFAPDNTRYVVPDIIVTNQRGRWVATLNPAVQPQVRINRAYAEIAASRSGCGASFS
RLLQEARWLLRNVEQRFNTIQRVADAIVARQRVFFSYGEAAMKPMALKDIATELGLHESTVCRVTNGKYMATPRGLFEFK
YFFSRQLATDDGGSCSATAIRALLKELIAAEDPQTPLSDAQLARMLAEQGLRLARRTVTKYRTLMRVPSVELRRVAGRAP
NAAEAGA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=491, Percent_Identity=40.9368635437882, Blast_Score=362, Evalue=1e-101,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 53483; Mature: 53352

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLGMELRLRQHLAITPQIQQALRLLQLSSQEFTQEVEQAVTSNPFLEEDPDAPPPGPAA
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
AMPLAPAASSSEPRAETVDGGDGEGSDAPVSPALHEIGRSGGGGGGDEEHDWTEWTEAPI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHCCCH
SLRDNLRNQLLLSPMSDRDRTLAHMVIDELTDAGYLETPLEELADLISPEEEVDVHELSA
HHHHHHHHHEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHCCHHHHHH
ALKLVQQLDPPGIAARSLQECLLLQLQALPASTPNLSLALELVGQHFELLARREFTRLQQ
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLSCNENALHGARALIRTLDPRPGRCFAPDNTRYVVPDIIVTNQRGRWVATLNPAVQPQV
HHCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCEEECCEEEECCCCCEEEEECCCCCCHH
RINRAYAEIAASRSGCGASFSRLLQEARWLLRNVEQRFNTIQRVADAIVARQRVFFSYGE
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAMKPMALKDIATELGLHESTVCRVTNGKYMATPRGLFEFKYFFSRQLATDDGGSCSATA
HHHCCHHHHHHHHHHCCCCCCEEEEECCEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHH
IRALLKELIAAEDPQTPLSDAQLARMLAEQGLRLARRTVTKYRTLMRVPSVELRRVAGRA
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCC
PNAAEAGA
CCCCCCCC
>Mature Secondary Structure 
SLGMELRLRQHLAITPQIQQALRLLQLSSQEFTQEVEQAVTSNPFLEEDPDAPPPGPAA
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
AMPLAPAASSSEPRAETVDGGDGEGSDAPVSPALHEIGRSGGGGGGDEEHDWTEWTEAPI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHCCCH
SLRDNLRNQLLLSPMSDRDRTLAHMVIDELTDAGYLETPLEELADLISPEEEVDVHELSA
HHHHHHHHHEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHCCHHHHHH
ALKLVQQLDPPGIAARSLQECLLLQLQALPASTPNLSLALELVGQHFELLARREFTRLQQ
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLSCNENALHGARALIRTLDPRPGRCFAPDNTRYVVPDIIVTNQRGRWVATLNPAVQPQV
HHCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCEEECCEEEECCCCCEEEEECCCCCCHH
RINRAYAEIAASRSGCGASFSRLLQEARWLLRNVEQRFNTIQRVADAIVARQRVFFSYGE
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAMKPMALKDIATELGLHESTVCRVTNGKYMATPRGLFEFKYFFSRQLATDDGGSCSATA
HHHCCHHHHHHHHHHCCCCCCEEEEECCEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHH
IRALLKELIAAEDPQTPLSDAQLARMLAEQGLRLARRTVTKYRTLMRVPSVELRRVAGRA
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCC
PNAAEAGA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1417413 [H]