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The map label for this gene is ampD1 [H]

Identifier: 119898070

GI number: 119898070

Start: 1945848

End: 1946708

Strand: Direct

Name: ampD1 [H]

Synonym: azo1779

Alternate gene names: 119898070

Gene position: 1945848-1946708 (Clockwise)

Preceding gene: 119898069

Following gene: 119898071

Centisome position: 44.47

GC content: 60.63

Gene sequence:

>861_bases
ATGGGCGATCTGAGTGTTGCAAATCACCGCTTGGCCGGAAAGGTGAAGGGCAAGAGCATTGGCTTCAGCGCTTCACCCAA
CCACAACGGAATCATCAATCCCAAATTCGTCGTCTTTCACTATACCGCCTGCTCCCATGCCGACGCGTCCGCGGCATTCA
TGAACGCGTCGGGCAATCGGCGAGTGTCAGCCCATCTGCTGGTCGATAGCGATGGCAGCATCACGCAATTCGTCGATTTC
AATCTGCGGGCTTGGCACGCGGGCGTCAGTGAGTGGGAGGACTACCGCGATCTCAATTCGCACTCCATCGGAGTGGAAAT
CGTCAATTACGGCTACCTGCTGAAGAACTCTGCTGGAGGTTTCACCCTGTCCAACGGTAGGAGCAGCCCGTTCACTCCGG
CGGAAGTTGTCGAAGCTCGGCACCGCAAAGAGGCGGTCCGCCACCAGTACTGGCACGCCTTCACGCCCGAACAACTGGAA
ACCTGCGAAGCGCTGGTCGAGGTGTTGTTCGATGCGTATGCACTGAGCGATGTCCTCGGCCACGACGACATCGCCCCCAC
CCGAAAGGTCGACCCCGGCCCGGCATTTCCGATGGATCGAATCAAGAGCCGTGCCGTTGGACGGGATTCCGATATCGAGA
TGGTGGAGGCGGTGTACGTCGCCGTGCCGCGACTGAATATCCGCGAGGGCGCTGGAACAGGCTACCGCCCGGTTCGCGAA
CCGCTGACCAAGCACACCCGGCTGGTTGTGATCCAGCGCAGCGGTGGCTGGATCAACGTGGAGGTGGACGGGCCCGGACA
GGTAAAGGGGTGGGTGTGGGGTGAATACACCCGCAGCTCGCCGCTGCAGGGAGATGGCTGA

Upstream 100 bases:

>100_bases
GCTGAACCGACGCCCGGCGATCGCCAGCAGGTGGCGCCAGCCGCTCCGGCGCCGGACGCGGTTGGTTGATCCACGTAAAT
CGGTAACCCGCGAGGACAAC

Downstream 100 bases:

>100_bases
CGGTCCGCGCCAGCCTTGGGTTTTTCAATCCGGGAACGCGGATCCAGAAGAGAACCGCGGCCCGCATCAGTTGTGCGACG
GGAATTCCCCCGTCGCCATT

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MGDLSVANHRLAGKVKGKSIGFSASPNHNGIINPKFVVFHYTACSHADASAAFMNASGNRRVSAHLLVDSDGSITQFVDF
NLRAWHAGVSEWEDYRDLNSHSIGVEIVNYGYLLKNSAGGFTLSNGRSSPFTPAEVVEARHRKEAVRHQYWHAFTPEQLE
TCEALVEVLFDAYALSDVLGHDDIAPTRKVDPGPAFPMDRIKSRAVGRDSDIEMVEAVYVAVPRLNIREGAGTGYRPVRE
PLTKHTRLVVIQRSGGWINVEVDGPGQVKGWVWGEYTRSSPLQGDG

Sequences:

>Translated_286_residues
MGDLSVANHRLAGKVKGKSIGFSASPNHNGIINPKFVVFHYTACSHADASAAFMNASGNRRVSAHLLVDSDGSITQFVDF
NLRAWHAGVSEWEDYRDLNSHSIGVEIVNYGYLLKNSAGGFTLSNGRSSPFTPAEVVEARHRKEAVRHQYWHAFTPEQLE
TCEALVEVLFDAYALSDVLGHDDIAPTRKVDPGPAFPMDRIKSRAVGRDSDIEMVEAVYVAVPRLNIREGAGTGYRPVRE
PLTKHTRLVVIQRSGGWINVEVDGPGQVKGWVWGEYTRSSPLQGDG
>Mature_285_residues
GDLSVANHRLAGKVKGKSIGFSASPNHNGIINPKFVVFHYTACSHADASAAFMNASGNRRVSAHLLVDSDGSITQFVDFN
LRAWHAGVSEWEDYRDLNSHSIGVEIVNYGYLLKNSAGGFTLSNGRSSPFTPAEVVEARHRKEAVRHQYWHAFTPEQLET
CEALVEVLFDAYALSDVLGHDDIAPTRKVDPGPAFPMDRIKSRAVGRDSDIEMVEAVYVAVPRLNIREGAGTGYRPVREP
LTKHTRLVVIQRSGGWINVEVDGPGQVKGWVWGEYTRSSPLQGDG

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=190, Percent_Identity=30.5263157894737, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 31412; Mature: 31281

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDLSVANHRLAGKVKGKSIGFSASPNHNGIINPKFVVFHYTACSHADASAAFMNASGNR
CCCCCCCCCEEEEEECCCEECCCCCCCCCCCCCCEEEEEEEECCCCCCCCEEEECCCCCC
RVSAHLLVDSDGSITQFVDFNLRAWHAGVSEWEDYRDLNSHSIGVEIVNYGYLLKNSAGG
EEEEEEEEECCCCEEEEEECCEEEECCCHHHHHHHHCCCCCCCEEEEEEEEEEEECCCCC
FTLSNGRSSPFTPAEVVEARHRKEAVRHQYWHAFTPEQLETCEALVEVLFDAYALSDVLG
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
HDDIAPTRKVDPGPAFPMDRIKSRAVGRDSDIEMVEAVYVAVPRLNIREGAGTGYRPVRE
CCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCHHHH
PLTKHTRLVVIQRSGGWINVEVDGPGQVKGWVWGEYTRSSPLQGDG
HHCCCEEEEEEEECCCEEEEEECCCCCEEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
GDLSVANHRLAGKVKGKSIGFSASPNHNGIINPKFVVFHYTACSHADASAAFMNASGNR
CCCCCCCCEEEEEECCCEECCCCCCCCCCCCCCEEEEEEEECCCCCCCCEEEECCCCCC
RVSAHLLVDSDGSITQFVDFNLRAWHAGVSEWEDYRDLNSHSIGVEIVNYGYLLKNSAGG
EEEEEEEEECCCCEEEEEECCEEEECCCHHHHHHHHCCCCCCCEEEEEEEEEEEECCCCC
FTLSNGRSSPFTPAEVVEARHRKEAVRHQYWHAFTPEQLETCEALVEVLFDAYALSDVLG
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
HDDIAPTRKVDPGPAFPMDRIKSRAVGRDSDIEMVEAVYVAVPRLNIREGAGTGYRPVRE
CCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCHHHH
PLTKHTRLVVIQRSGGWINVEVDGPGQVKGWVWGEYTRSSPLQGDG
HHCCCEEEEEEEECCCEEEEEECCCCCEEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]