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The map label for this gene is cls2 [H]

Identifier: 119898044

GI number: 119898044

Start: 1919438

End: 1920847

Strand: Direct

Name: cls2 [H]

Synonym: azo1753

Alternate gene names: 119898044

Gene position: 1919438-1920847 (Clockwise)

Preceding gene: 119898043

Following gene: 119898045

Centisome position: 43.86

GC content: 70.35

Gene sequence:

>1410_bases
ATGACATCAGGATCAAGACGGCGCCGTAAGACGCCGCGCCTCGTCCTCTGCCTCGCGGTGCTGCTGGCGGCCTGCGTCAG
CGGTCCGGCGGTCGATCTGCCGGACGCCGGCGCCGCGGTGCCGCTGGCGGCGGTGCGGGTGGAGGACGAACGTGGCCCCT
TGGGCGCTGCGCGCGCACAGGCCGCCATCCAGCGCATCGCGCGCGAGGATGCGTCCGGCCTGCTGCGCCATCACCTCACC
CATGTCGAAGGCGCCGTGACCCAGCCGCTGGTGCTCGGCAACGAAGCCCATCTCCTGATCGACGGCCCGCAGACCGAGGC
CGCCATGTTCGCCGCGATCGGCGCGGCACGCCGCTCGGTGGACATCGAAACCTACATCCTGGAAGCGGAGGGCGCGGGCG
CGAGGCTTGCCGCGCTGCTCGAAAAACGCCGCGCCGAGGGCATCCGGGTGCGCGTGCTGTACGACAGCGTCGGGTCGCTG
GCGACCCCGCGCGAGTACTTCGACCGCCTGCGCGCCGTCGGCGTCGCGGTGTGCGAATTCAACCCGGTCAATCCGCTGCA
ACTGCAGCGCCACAGCCGGCTCAACATCAATAACCGCGACCATCGCAAGCTGATGGTGGTGGACGGCAGCACCGCCTTCA
CCGGCGGCATCAACATCAGCGCGGTGTACAGCTCCGGCTCCTTCAGCCGCAAGAAGCGCGCGCCGCCCGATCCCAAGGCC
GGCTGGCGCGACACCCACGTGATGGCGCGCGGTCCGGTCGCGATGCAGTTCCAGCAGCTGTTCGACGACGCATGGCGCAC
CCAGGGCTGCCACCCGGCGGACTCCGGCCTGCGCGAAGCCGCCGCGCCGGCCCGCGCCGGGAACATGGCGATGCGCACGG
TGGCGGCCGATCCGCTGATGGAGCGCAGCGAACTCTACGTGGCGCTGCTGTCGGCGATCGACCACGCGCGCCGGCGGGTG
TGGCTGACCTACGGCTATTTCGTGCCGGACGAGCGCACCGTGGCGGCGCTGCAGTCGGCCGCCGGCCGCGGCGTGGATGT
GCGCATGGTGCTGCCGGGCTTCAGCGATTTCTGGGCGCCCTTCCATGCCGGGCGCTCGCACTACAGCGCGCTGCTTGAAG
CCGGGGTGCGCATCTACGAGCGCCGCGACGCGCTGCTGCATGCCAAGACCGCGGTCATCGACGGGGTGTGGTCCAGCGTG
GGCTCCACCAATCTCGACTGGCGCAGCTTCGTGCACAACTTCGAGGTGGATCTGCTGGTGCTCGACGGCGACTTCGCCGA
CGAGCTGGAGCAGCTTTTCCGCATGGACATGGACGCCTCGCACGAGGTGAGCGCGCGCGAATGGAAACGGCGCGACATCG
GCACCCGGATGCTGGAGTGGTTCGCGCGGCGCTGGGAGTACCTGCTGTGA

Upstream 100 bases:

>100_bases
CGCGGCACCGTGCAGCTCAGCGGCTTCGTCGCCACCCAGGCCGAATCGCGGGCCGCGGTGGAGGCGGCGCGTTCCGTCAA
GGGCGTGCAGGCCGTGCAGA

Downstream 100 bases:

>100_bases
GGCGTGCGGGCCGCGACGGCCGCGCGCGGATGACCGGAGGCGGGCGCGATGACCTTGCTGAAAGCCTTGCACCTCGGCGC
GCTGATCTGCTGGTGCGGCA

Product: putative cardiolipin synthetase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTSGSRRRRKTPRLVLCLAVLLAACVSGPAVDLPDAGAAVPLAAVRVEDERGPLGAARAQAAIQRIAREDASGLLRHHLT
HVEGAVTQPLVLGNEAHLLIDGPQTEAAMFAAIGAARRSVDIETYILEAEGAGARLAALLEKRRAEGIRVRVLYDSVGSL
ATPREYFDRLRAVGVAVCEFNPVNPLQLQRHSRLNINNRDHRKLMVVDGSTAFTGGINISAVYSSGSFSRKKRAPPDPKA
GWRDTHVMARGPVAMQFQQLFDDAWRTQGCHPADSGLREAAAPARAGNMAMRTVAADPLMERSELYVALLSAIDHARRRV
WLTYGYFVPDERTVAALQSAAGRGVDVRMVLPGFSDFWAPFHAGRSHYSALLEAGVRIYERRDALLHAKTAVIDGVWSSV
GSTNLDWRSFVHNFEVDLLVLDGDFADELEQLFRMDMDASHEVSAREWKRRDIGTRMLEWFARRWEYLL

Sequences:

>Translated_469_residues
MTSGSRRRRKTPRLVLCLAVLLAACVSGPAVDLPDAGAAVPLAAVRVEDERGPLGAARAQAAIQRIAREDASGLLRHHLT
HVEGAVTQPLVLGNEAHLLIDGPQTEAAMFAAIGAARRSVDIETYILEAEGAGARLAALLEKRRAEGIRVRVLYDSVGSL
ATPREYFDRLRAVGVAVCEFNPVNPLQLQRHSRLNINNRDHRKLMVVDGSTAFTGGINISAVYSSGSFSRKKRAPPDPKA
GWRDTHVMARGPVAMQFQQLFDDAWRTQGCHPADSGLREAAAPARAGNMAMRTVAADPLMERSELYVALLSAIDHARRRV
WLTYGYFVPDERTVAALQSAAGRGVDVRMVLPGFSDFWAPFHAGRSHYSALLEAGVRIYERRDALLHAKTAVIDGVWSSV
GSTNLDWRSFVHNFEVDLLVLDGDFADELEQLFRMDMDASHEVSAREWKRRDIGTRMLEWFARRWEYLL
>Mature_468_residues
TSGSRRRRKTPRLVLCLAVLLAACVSGPAVDLPDAGAAVPLAAVRVEDERGPLGAARAQAAIQRIAREDASGLLRHHLTH
VEGAVTQPLVLGNEAHLLIDGPQTEAAMFAAIGAARRSVDIETYILEAEGAGARLAALLEKRRAEGIRVRVLYDSVGSLA
TPREYFDRLRAVGVAVCEFNPVNPLQLQRHSRLNINNRDHRKLMVVDGSTAFTGGINISAVYSSGSFSRKKRAPPDPKAG
WRDTHVMARGPVAMQFQQLFDDAWRTQGCHPADSGLREAAAPARAGNMAMRTVAADPLMERSELYVALLSAIDHARRRVW
LTYGYFVPDERTVAALQSAAGRGVDVRMVLPGFSDFWAPFHAGRSHYSALLEAGVRIYERRDALLHAKTAVIDGVWSSVG
STNLDWRSFVHNFEVDLLVLDGDFADELEQLFRMDMDASHEVSAREWKRRDIGTRMLEWFARRWEYLL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=379, Percent_Identity=29.8153034300792, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1787007, Length=344, Percent_Identity=30.5232558139535, Blast_Score=137, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 51820; Mature: 51688

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00018 EF_HAND_1 ; PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSGSRRRRKTPRLVLCLAVLLAACVSGPAVDLPDAGAAVPLAAVRVEDERGPLGAARAQ
CCCCCCHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHH
AAIQRIAREDASGLLRHHLTHVEGAVTQPLVLGNEAHLLIDGPQTEAAMFAAIGAARRSV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEECCCHHHHHHHHHHHHHHCC
DIETYILEAEGAGARLAALLEKRRAEGIRVRVLYDSVGSLATPREYFDRLRAVGVAVCEF
EEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHEEEEEC
NPVNPLQLQRHSRLNINNRDHRKLMVVDGSTAFTGGINISAVYSSGSFSRKKRAPPDPKA
CCCCHHHHHHCCCCCCCCCCCCEEEEEECCCEEECCEEEEEEECCCCCCCCCCCCCCCCC
GWRDTHVMARGPVAMQFQQLFDDAWRTQGCHPADSGLREAAAPARAGNMAMRTVAADPLM
CCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHCCHH
ERSELYVALLSAIDHARRRVWLTYGYFVPDERTVAALQSAAGRGVDVRMVLPGFSDFWAP
HHHHHHHHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHH
FHAGRSHYSALLEAGVRIYERRDALLHAKTAVIDGVWSSVGSTNLDWRSFVHNFEVDLLV
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEEEE
LDGDFADELEQLFRMDMDASHEVSAREWKRRDIGTRMLEWFARRWEYLL
ECCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TSGSRRRRKTPRLVLCLAVLLAACVSGPAVDLPDAGAAVPLAAVRVEDERGPLGAARAQ
CCCCCHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHH
AAIQRIAREDASGLLRHHLTHVEGAVTQPLVLGNEAHLLIDGPQTEAAMFAAIGAARRSV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEECCCHHHHHHHHHHHHHHCC
DIETYILEAEGAGARLAALLEKRRAEGIRVRVLYDSVGSLATPREYFDRLRAVGVAVCEF
EEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHEEEEEC
NPVNPLQLQRHSRLNINNRDHRKLMVVDGSTAFTGGINISAVYSSGSFSRKKRAPPDPKA
CCCCHHHHHHCCCCCCCCCCCCEEEEEECCCEEECCEEEEEEECCCCCCCCCCCCCCCCC
GWRDTHVMARGPVAMQFQQLFDDAWRTQGCHPADSGLREAAAPARAGNMAMRTVAADPLM
CCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHCCHH
ERSELYVALLSAIDHARRRVWLTYGYFVPDERTVAALQSAAGRGVDVRMVLPGFSDFWAP
HHHHHHHHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHH
FHAGRSHYSALLEAGVRIYERRDALLHAKTAVIDGVWSSVGSTNLDWRSFVHNFEVDLLV
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEEEE
LDGDFADELEQLFRMDMDASHEVSAREWKRRDIGTRMLEWFARRWEYLL
ECCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA