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The map label for this gene is ygfZ [C]

Identifier: 119897884

GI number: 119897884

Start: 1749321

End: 1750349

Strand: Reverse

Name: ygfZ [C]

Synonym: azo1593

Alternate gene names: 119897884

Gene position: 1750349-1749321 (Counterclockwise)

Preceding gene: 119897891

Following gene: 119897883

Centisome position: 40.0

GC content: 68.8

Gene sequence:

>1029_bases
ATGACCGCCTGGACCGATCACCTCGCGCTGGAAGGCGCCAAGCTCGACACCCTGTCCGTGAGTTTTGCGGACGCCCGATC
CGATGCCGCCGCGCTTGAGCGGGGTACCGTCGTCGTTCCGCTGTTGCACCTGGGGCTGATCCGCAGCGTCGGCCCCGACT
CGACGACCTTCCTGCACAACCTTTTCTCCAACGACGTCGCCAAGCTGCCCGCCGACGGCGCGCAGTGGAGCAGCTTCAAC
AGCCCGAAGGGCCGCATGCTGGCCAGCCTGCTGCTGTGGCCGGAGGCCGAAGGCCATTCACTCGTCATGGCTGCGGACAT
CCTGCCGGCGATGCTGAAGAAGCTGTCGATGTACGTGCTGCGCAGCAAGGTCAAGCTGAACGACGCCGGTGAAACCACGG
TGCTGATCGGCGTGGCCGGCGGCGACGCGGCGGCCGTGCTCCAGGGCGCCGGGTTGCCGGTGCCGGCAGCGCCGATGCAA
CAGGTGTCCGCCGCGGCAGGCCGTGTCGTGCGCGTGGGCGAACAGGCCTTTGTCCTTGCGATCGACAGCGCGGCAGCGAG
CAGCGTGTTTACCGCCCTTGTCGCGGCCGGCGCCCACAAGGCCGGCACCGCCGCCTGGCAACTGGCGATGATCCGCGCGG
GCGTGCCGCTGATCACCGCGCCGACCCAGGAAGAATTCGTCGCCCAGATGCTGAATTACGACCTCATCGGCGGCGTCAAT
TTCAAGAAGGGCTGCTACCCCGGGCAGGAGATCGTGGCCCGCACGCAGTACCTCGGCACCGTCAAGAAGCGCCTCTATCG
CGTGGCGCTCGATGCCGACAGCGTGCCCACGCCCGGCAGCGACCTGTATGCGCCCGACTTCGGCGAGCAGTCCGCCGGCA
AGGTGGTCAACGTGGCGCCGTCGCCCGAAGGCGGCTACGAAGCCCTGGCGGTACTGCAGAACACCAGCGTCGAAGGCGGC
CAGCTGCGCTACGGAAGCCCGGCCGGAGCGCCGGTGCGCGTCCTCGACCTGCCCTACCCGCTGCCCTGA

Upstream 100 bases:

>100_bases
CTATAATACTTCATTGCTCCAGTGCCGCCGCGGACGCCGGCGGGCGCTGTGCGTCCCGCGTGTCGCCGCCCCTTCCCTCA
ACCTTTGTGCAGAATCCGAC

Downstream 100 bases:

>100_bases
TCCAGGAGACGCCATGAGCGATGCCACCGCCAGCCTGTCCACGACCGTCCAGTACTTCATCTACTACCGGGTGCGCGAAG
AACTGGACGCCGAGGTTGCC

Product: aminomethyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MTAWTDHLALEGAKLDTLSVSFADARSDAAALERGTVVVPLLHLGLIRSVGPDSTTFLHNLFSNDVAKLPADGAQWSSFN
SPKGRMLASLLLWPEAEGHSLVMAADILPAMLKKLSMYVLRSKVKLNDAGETTVLIGVAGGDAAAVLQGAGLPVPAAPMQ
QVSAAAGRVVRVGEQAFVLAIDSAAASSVFTALVAAGAHKAGTAAWQLAMIRAGVPLITAPTQEEFVAQMLNYDLIGGVN
FKKGCYPGQEIVARTQYLGTVKKRLYRVALDADSVPTPGSDLYAPDFGEQSAGKVVNVAPSPEGGYEALAVLQNTSVEGG
QLRYGSPAGAPVRVLDLPYPLP

Sequences:

>Translated_342_residues
MTAWTDHLALEGAKLDTLSVSFADARSDAAALERGTVVVPLLHLGLIRSVGPDSTTFLHNLFSNDVAKLPADGAQWSSFN
SPKGRMLASLLLWPEAEGHSLVMAADILPAMLKKLSMYVLRSKVKLNDAGETTVLIGVAGGDAAAVLQGAGLPVPAAPMQ
QVSAAAGRVVRVGEQAFVLAIDSAAASSVFTALVAAGAHKAGTAAWQLAMIRAGVPLITAPTQEEFVAQMLNYDLIGGVN
FKKGCYPGQEIVARTQYLGTVKKRLYRVALDADSVPTPGSDLYAPDFGEQSAGKVVNVAPSPEGGYEALAVLQNTSVEGG
QLRYGSPAGAPVRVLDLPYPLP
>Mature_341_residues
TAWTDHLALEGAKLDTLSVSFADARSDAAALERGTVVVPLLHLGLIRSVGPDSTTFLHNLFSNDVAKLPADGAQWSSFNS
PKGRMLASLLLWPEAEGHSLVMAADILPAMLKKLSMYVLRSKVKLNDAGETTVLIGVAGGDAAAVLQGAGLPVPAAPMQQ
VSAAAGRVVRVGEQAFVLAIDSAAASSVFTALVAAGAHKAGTAAWQLAMIRAGVPLITAPTQEEFVAQMLNYDLIGGVNF
KKGCYPGQEIVARTQYLGTVKKRLYRVALDADSVPTPGSDLYAPDFGEQSAGKVVNVAPSPEGGYEALAVLQNTSVEGGQ
LRYGSPAGAPVRVLDLPYPLP

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication [H]

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family [H]

Homologues:

Organism=Escherichia coli, GI1789265, Length=235, Percent_Identity=30.2127659574468, Blast_Score=108, Evalue=6e-25,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017703
- InterPro:   IPR006222 [H]

Pfam domain/function: PF01571 GCV_T [H]

EC number: NA

Molecular weight: Translated: 35570; Mature: 35439

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAWTDHLALEGAKLDTLSVSFADARSDAAALERGTVVVPLLHLGLIRSVGPDSTTFLHN
CCCCCCHHEECCCCEEEEEEEHHHCCCHHHHHHCCCEEHHHHHHHHHHHCCCCHHHHHHH
LFSNDVAKLPADGAQWSSFNSPKGRMLASLLLWPEAEGHSLVMAADILPAMLKKLSMYVL
HHHCHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHH
RSKVKLNDAGETTVLIGVAGGDAAAVLQGAGLPVPAAPMQQVSAAAGRVVRVGEQAFVLA
HHHHEECCCCCEEEEEEECCCCHHHHHCCCCCCCCCCHHHHHHHHHCCEEEECCCEEEEE
IDSAAASSVFTALVAAGAHKAGTAAWQLAMIRAGVPLITAPTQEEFVAQMLNYDLIGGVN
ECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEECCCHHHHHHHHHCCHHCCCCC
FKKGCYPGQEIVARTQYLGTVKKRLYRVALDADSVPTPGSDLYAPDFGEQSAGKVVNVAP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCEEEECC
SPEGGYEALAVLQNTSVEGGQLRYGSPAGAPVRVLDLPYPLP
CCCCCHHEEEEEECCCCCCCEEEECCCCCCCEEEEECCCCCC
>Mature Secondary Structure 
TAWTDHLALEGAKLDTLSVSFADARSDAAALERGTVVVPLLHLGLIRSVGPDSTTFLHN
CCCCCHHEECCCCEEEEEEEHHHCCCHHHHHHCCCEEHHHHHHHHHHHCCCCHHHHHHH
LFSNDVAKLPADGAQWSSFNSPKGRMLASLLLWPEAEGHSLVMAADILPAMLKKLSMYVL
HHHCHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHH
RSKVKLNDAGETTVLIGVAGGDAAAVLQGAGLPVPAAPMQQVSAAAGRVVRVGEQAFVLA
HHHHEECCCCCEEEEEEECCCCHHHHHCCCCCCCCCCHHHHHHHHHCCEEEECCCEEEEE
IDSAAASSVFTALVAAGAHKAGTAAWQLAMIRAGVPLITAPTQEEFVAQMLNYDLIGGVN
ECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEECCCHHHHHHHHHCCHHCCCCC
FKKGCYPGQEIVARTQYLGTVKKRLYRVALDADSVPTPGSDLYAPDFGEQSAGKVVNVAP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCEEEECC
SPEGGYEALAVLQNTSVEGGQLRYGSPAGAPVRVLDLPYPLP
CCCCCHHEEEEEECCCCCCCEEEECCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA